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comstat software  (MathWorks Inc)


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    Structured Review

    MathWorks Inc comstat software
    Comstat Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/comstat/pm37914231-80-9-11
    Average 90 stars, based on 1 article reviews
    comstat software - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Bacteria‐Responsive Multidrug Delivery Nanosystem for Combating Long‐Term Biofilm‐Associated Infections
    Article Snippet: Microbial biofilm formation on implantable devices causes chronic infections that cannot be treated with existing antimicrobials.. Quorum sensing inhibitors (QSIs) have recently emerged as novel antimicrobials for the prevention of biofilm formation.. But blocking QS alone is insufficient to inhibit biofilmassociated chronic infections.

    Software:

    Article Title: Bacteria‐Responsive Multidrug Delivery Nanosystem for Combating Long‐Term Biofilm‐Associated Infections
    Article Snippet: Microbial biofilm formation on implantable devices causes chronic infections that cannot be treated with existing antimicrobials.. Quorum sensing inhibitors (QSIs) have recently emerged as novel antimicrobials for the prevention of biofilm formation.. But blocking QS alone is insufficient to inhibit biofilmassociated chronic infections.

    Article Title: D-Amino Acids Trigger Disassembly of Dual-Species Biofilms by Bacillus subtilis and Escherichia coli
    Article Snippet: In a large part of ecological niches, bacterial species are united in communities and develop together in the form of heterogeneous structures called biofilms, which are formed on surfaces of different nature s In this study, we evaluated effects of the D-alanine in the cultural medium on the biofilm development and architecture of biofilms of the bacterium (B. subtilis) as well as their interactions with Escherichia coli K-12 1655 strain.. Effects of the D-alanine in the cultural medium on bacterial biofilm formation and the mechanisms were analyzed by the crystal violet staining method combined with cultivated microbial analysis, and confocal laser scanning microscopy.. A 24 h mature Bacillus subtilis and Escherichia coli dual-species biofilms were exposed to different concentrations of D-alanine in the cultural medium.

    Article Title: Trade-offs of lipid remodeling in a marine predator–prey interaction in response to phosphorus limitation
    Article Snippet: Finally, cells were stained with DAPI (5 μg/mL) and ConA TRITC conjugate (20 μg/mL; Thermo Fisher Scientific) ( ) and mounted with a drop of Mowiol antifade before observation using a confocal laser scanning microscope (CLSM; Zeiss LSM 880). .. Bacterial biovolumes and the biovolume of ConA-stained glycoconjugates in CLSM images were determined by using five images for each of the three replicates using COMSTAT software developed in MATLAB R2015a (MathWorks), as described ( ). ..

    Article Title: Bioinspired synthesis of gold nanoparticles from Hemidesmus indicus L. root extract and their antibiofilm efficacy against Pseudomonas aeruginosa
    Article Snippet: Pseudomonas aeruginosa, is one the major causative agents for nosocomial and biofilm mediated infections.. The present study is an attempt to disarm the biofilm forming potential of PAO1 using gold nanoparticles biosynthesized from Hemidesmus indicus L. root extract (HiAuNPs).. The HiAuNPs were optimally synthesised using 1 mM HAuCl4 at 70 C for 20 min with a maximum surface plasmon resonance (SPR) at 531 nm.

    Article Title: A novel class of sulfur-containing aminolipids widespread in marine roseobacters
    Article Snippet: At each time point (3, 24, and 48 h), biofilms were washed to remove non-adherent bacteria and fixed using formalin 3.5% (v/v) for 20 min. Bacteria were stained using DAPI (5 μg• mL −1 , Sigma-Aldrich, Darmstadt, Germany) and coverslips were mounted with a drop of Mowiol antifade before observation using confocal laser scanning microscopy (CLSM) (Zeiss LSM 880, Göttingen, Germany). .. The biovolume and the average thickness of the biofilms were determined using COMSTAT software developed in MATLAB R2017a (MathWorks, Natick, MA, United States) as described previously [ , ]. .. To test for statistically significant differences between the wild-type strain and the salA mutant, a t -test was performed using SPSS 13.0 (IBM, Armonk, NY, USA).

    Article Title: Combined effect of apigenin and reduced graphene oxide against Enterococcus faecalis biofilms.
    Article Snippet: Images were taken randomly by confocal laser scanning microscopy (CLSM) (n = 9) using an LSM 510 META microscope (Carl Zeiss, Jena, Germany). .. The bio-volume of live/dead E. faecalis in the biofilms was analyzed using the COMSTAT software (MATLAB; The MathWorks Inc., Natick, MA, USA). ..

    Article Title: Combined effect of electrical energy and graphene oxide on Enterococcus faecalis biofilms.
    Article Snippet: Then, images of live (green) and dead (red) E. faecalis were obtained by CLSM (3 images/specimen, n=9) using an LSM 510 META microscope (Carl Zeiss, Jena, Germany). .. The bio-volume of live/dead E. faecalis was analyzed using COMSTAT software (MATLAB, MathWorks, Natick, MA, USA). .. Scanning electron microscopy (SEM) assay The 7-day-old E. faecalis biofilms were washed 3 times with PBS after treatment and were fixed with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences) in 0.05 M sodium carcodylate The bacteria in the biofilms were stained with live (green) and dead (red) dyes. (a) Control, (b) 0.1% NaOCl, (c) 0.2% NaOCl, (d) 0.5% NaOCl, (e) 0.2% NaOCl combined with electrical energy, and (f) 0.2% NaOCl combined with electrical energy and GO. (g) The live bio-volume (black bar) and dead bio-volume (white bar) are presented.

    Article Title: Role of Iron Homeostasis in the Multispecies Biofilm Formation
    Article Snippet: Iron acquisition is of fundamental importance for microorganisms, since this metal is generally poorly bioavailable under natural conditions.. Fe is mostly present as a ferric form in soils, which strongly limits its bioavailability, while most soil bacteria are tightly packed within multicellular communities named biofilms.. This research showed that biofilm formation by a gram-positive bacterium Bacillus subtilis during the interactions with other microbial species was both essential to ensure Fe uptake from the environment and to maintain the cellular Fe homeostasis.

    Confocal Laser Scanning Microscopy:

    Article Title: D-Amino Acids Trigger Disassembly of Dual-Species Biofilms by Bacillus subtilis and Escherichia coli
    Article Snippet: In a large part of ecological niches, bacterial species are united in communities and develop together in the form of heterogeneous structures called biofilms, which are formed on surfaces of different nature s In this study, we evaluated effects of the D-alanine in the cultural medium on the biofilm development and architecture of biofilms of the bacterium (B. subtilis) as well as their interactions with Escherichia coli K-12 1655 strain.. Effects of the D-alanine in the cultural medium on bacterial biofilm formation and the mechanisms were analyzed by the crystal violet staining method combined with cultivated microbial analysis, and confocal laser scanning microscopy.. A 24 h mature Bacillus subtilis and Escherichia coli dual-species biofilms were exposed to different concentrations of D-alanine in the cultural medium.

    Article Title: Trade-offs of lipid remodeling in a marine predator–prey interaction in response to phosphorus limitation
    Article Snippet: Finally, cells were stained with DAPI (5 μg/mL) and ConA TRITC conjugate (20 μg/mL; Thermo Fisher Scientific) ( ) and mounted with a drop of Mowiol antifade before observation using a confocal laser scanning microscope (CLSM; Zeiss LSM 880). .. Bacterial biovolumes and the biovolume of ConA-stained glycoconjugates in CLSM images were determined by using five images for each of the three replicates using COMSTAT software developed in MATLAB R2015a (MathWorks), as described ( ). ..

    Article Title: Role of Iron Homeostasis in the Multispecies Biofilm Formation
    Article Snippet: Iron acquisition is of fundamental importance for microorganisms, since this metal is generally poorly bioavailable under natural conditions.. Fe is mostly present as a ferric form in soils, which strongly limits its bioavailability, while most soil bacteria are tightly packed within multicellular communities named biofilms.. This research showed that biofilm formation by a gram-positive bacterium Bacillus subtilis during the interactions with other microbial species was both essential to ensure Fe uptake from the environment and to maintain the cellular Fe homeostasis.



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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    Image Search Results


    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.

    Journal: Journal of Bacteriology

    Article Title: FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance by Pseudomonas aeruginosa biofilms

    doi: 10.1128/jb.00503-24

    Figure Lengend Snippet: FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.

    Article Snippet: Quantitative analysis of the images was performed using COMSTAT MATLAB package and COMSTAT2 ( , ).

    Techniques: Mutagenesis, Expressing