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computer assisted analysis  (Olympus)


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    Structured Review

    Olympus computer assisted analysis
    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Computer Assisted Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 10108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer+imaging+software/cellSens+Imaging+Software/pmc12939340-97-8-16
    Average 99 stars, based on 10108 article reviews
    computer assisted analysis - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model"

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    Journal: Cells

    doi: 10.3390/cells15040360

    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Figure Legend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Techniques Used: Immunodetection, TUNEL Assay

    Related Articles

    Imaging:

    Article Title: Excessive ER-mitochondria coupling: A DRP1-driven mechanism underlying mitochondrial dysfunction and impaired autophagy in stress-induced depression-like behavior in mice
    Article Snippet: .. Nuclei were counterstained with DAPI before imaging with a confocal microscope (Olympus FV4000, Japan) and subsequent analysis using ImageJ or cellSens software. .. Brain sections were processed for PLA using the Duolink® system (Sigma-Aldrich, USA).

    Article Title: Systems and methods for training a statistical model to predict tissue characteristics for a pathology image
    Article Snippet: .. In some embodiments, a suitable imaging instrument includes an Olympus BX41 microscope in combination with a DP21 2 MP camera and CellSens software all available from Olympus Corporation (Center Valley, PA). .. Another suitable imaging instrument includes the Aperio ScanScope CS-0, Aperio AT2, and Aperio eSlide manager and web browser all available from Leica Biosystems, Inc. (Buffalo Grove, IL).

    Article Title: Multifaceted role of POU5F1P1 in regulating its parental stem cell gene, POU5F1
    Article Snippet: Hoechst (H342-Cellstain®- Hoechst 33342 solution; DOJINDO) was used as a nuclear marker when required. .. Cell images were acquired using the APX-100 Digital Imaging System (EVIDENT/OLYMPUS) and subsequently processed and analyzed using the OLYMPUS cellSens APEX4.2 software. ..

    Article Title: Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilization
    Article Snippet: .. The quantitative analysis of the sections was performed with a BX53 microscope and the imaging software cellSens Dimension (version 1.11; Olympus, Hamburg, Germany). ..

    Microscopy:

    Article Title: Excessive ER-mitochondria coupling: A DRP1-driven mechanism underlying mitochondrial dysfunction and impaired autophagy in stress-induced depression-like behavior in mice
    Article Snippet: .. Nuclei were counterstained with DAPI before imaging with a confocal microscope (Olympus FV4000, Japan) and subsequent analysis using ImageJ or cellSens software. .. Brain sections were processed for PLA using the Duolink® system (Sigma-Aldrich, USA).

    Article Title: Systems and methods for training a statistical model to predict tissue characteristics for a pathology image
    Article Snippet: .. In some embodiments, a suitable imaging instrument includes an Olympus BX41 microscope in combination with a DP21 2 MP camera and CellSens software all available from Olympus Corporation (Center Valley, PA). .. Another suitable imaging instrument includes the Aperio ScanScope CS-0, Aperio AT2, and Aperio eSlide manager and web browser all available from Leica Biosystems, Inc. (Buffalo Grove, IL).

    Article Title: Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilization
    Article Snippet: .. The quantitative analysis of the sections was performed with a BX53 microscope and the imaging software cellSens Dimension (version 1.11; Olympus, Hamburg, Germany). ..

    Software:

    Article Title: Excessive ER-mitochondria coupling: A DRP1-driven mechanism underlying mitochondrial dysfunction and impaired autophagy in stress-induced depression-like behavior in mice
    Article Snippet: .. Nuclei were counterstained with DAPI before imaging with a confocal microscope (Olympus FV4000, Japan) and subsequent analysis using ImageJ or cellSens software. .. Brain sections were processed for PLA using the Duolink® system (Sigma-Aldrich, USA).

    Article Title: Systems and methods for training a statistical model to predict tissue characteristics for a pathology image
    Article Snippet: .. In some embodiments, a suitable imaging instrument includes an Olympus BX41 microscope in combination with a DP21 2 MP camera and CellSens software all available from Olympus Corporation (Center Valley, PA). .. Another suitable imaging instrument includes the Aperio ScanScope CS-0, Aperio AT2, and Aperio eSlide manager and web browser all available from Leica Biosystems, Inc. (Buffalo Grove, IL).

    Article Title: ANKRD9 negatively regulates chicken myogenesis through ubiquitin-mediated regulation of IMPDH2
    Article Snippet: Images were captured using a fluorescence microscope (Olympus, Tokyo, Japan, IX73) for cell proliferation analysis. .. Image capture was controlled by the cellSens Dimension software (v5.1, Olympus). ..

    Article Title: Multifaceted role of POU5F1P1 in regulating its parental stem cell gene, POU5F1
    Article Snippet: .. Subsequent positive cell detection, cell counting, and fluorescence intensity measurements were performed using OLYMPUS CellSens APEX 4.2 software. .. Densitometric quantification of western blotting images was performed using ImageJ software (1.53q; (NIH, Bethesda, MD, USA).

    Article Title: Multifaceted role of POU5F1P1 in regulating its parental stem cell gene, POU5F1
    Article Snippet: Hoechst (H342-Cellstain®- Hoechst 33342 solution; DOJINDO) was used as a nuclear marker when required. .. Cell images were acquired using the APX-100 Digital Imaging System (EVIDENT/OLYMPUS) and subsequently processed and analyzed using the OLYMPUS cellSens APEX4.2 software. ..

    Article Title: Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilization
    Article Snippet: .. The quantitative analysis of the sections was performed with a BX53 microscope and the imaging software cellSens Dimension (version 1.11; Olympus, Hamburg, Germany). ..

    Cell Counting:

    Article Title: Multifaceted role of POU5F1P1 in regulating its parental stem cell gene, POU5F1
    Article Snippet: .. Subsequent positive cell detection, cell counting, and fluorescence intensity measurements were performed using OLYMPUS CellSens APEX 4.2 software. .. Densitometric quantification of western blotting images was performed using ImageJ software (1.53q; (NIH, Bethesda, MD, USA).

    Fluorescence:

    Article Title: Multifaceted role of POU5F1P1 in regulating its parental stem cell gene, POU5F1
    Article Snippet: .. Subsequent positive cell detection, cell counting, and fluorescence intensity measurements were performed using OLYMPUS CellSens APEX 4.2 software. .. Densitometric quantification of western blotting images was performed using ImageJ software (1.53q; (NIH, Bethesda, MD, USA).



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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay