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computer assisted imaging  (Bio-Rad)


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    Bio-Rad computer assisted imaging
    Computer Assisted Imaging, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer+imaging+software/Image+Lab+Software/pm41241330-100-9-14
    Average 99 stars, based on 35737 article reviews
    computer assisted imaging - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    other:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific).

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad).

    Software:

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution. .. Band intensities were quantified using Image Lab software (Bio-Rad). ..

    Article Title: Curcumin-Induced Vacuolizing Stress Reveals a Vulnerability Associated with NF1 Loss in Schwann Cells.
    Article Snippet: Membranes were probed with the following antibodies: anti-NF1 #sc-376886 (1:200) by Santa Cruz Biotechnology (Dallas, TX, USA), anti-PARP #10872500 (1:1000) by Roche, anti-NRF2 #PA58804 (1:1000) by Life Technologies, anti-TRX #EPR6110 (1:1000) by Abcam (Cambridge, UK), anti-SOD1 #TA50495 (1:1000) by OriGene Technologies, (Rockville, MD, USA), anti-cleaved caspase-3 (Asp175) #9661 (1:1000) by Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH-HRP #MA5-15738-HRP (1:5000) by Life Technologies. .. Bound antibodies were detected by chemiluminescence using WesternBright ECL (#K12045D50 Advansta, San Jose, CA, USA), and band intensities were quantified by densitometry with Image Lab software 5.0 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Staining:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Imaging:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Incubation:

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..



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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay