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mouse complement c3  (MedChemExpress)


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    Structured Review

    MedChemExpress mouse complement c3
    Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of <t>complement</t> <t>C3</t> proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.
    Mouse Complement C3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+c3/Complement+C3%2C+Mouse/pmc12926576-58-0-6
    Average 94 stars, based on 2 article reviews
    mouse complement c3 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy"

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.018

    Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of complement C3 proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.
    Figure Legend Snippet: Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of complement C3 proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.

    Techniques Used: In Vivo, Injection, Adsorption, Transfection, Immunopeptidomics

    In vivo splenic DC-specific transfection of mRNA/H 18 NPs and in vitro protein corona analysis of mRNA/H 18 NPs. (A) EGFP protein expression in splenic cell subsets of C57BL/6J mice 24 h post intravenous injection of different formulations. (B) The top 5 most abundant plasma proteins adsorbed on mRNA/H 18 NPs (C3: Complement C3; Ighm: Immunoglobulin heavy constant mu; Hbat1: Alpha-globin; Itih4: Inter alpha-trypsin inhibitor, heavy chain 4; Cnn2: Calponin). (C) Heatmap plot of major proteins in the protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. PBS group was used as a negative control. (D) Quantification of major adsorbed protein categories of different formulations. (E) Complement C3 abundance in protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. (F) Bioluminescence images of major organs and (G) Quantification of total bioluminescence flux in the spleen from C57BL/6J mice 6 h after intravenous injection of mLuc/H 18 NPs (mLuc dose of 0.25 mg kg −1 ). Mice were pre-treated with cobra venom factor (CVF) or PBS. (H) Fluorescence quantification of Cy5 mRNA delivered by uncoated or complement C3-coated Cy5-mRNA/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. (I) Bioluminescence intensity of luciferase protein translated from mRNA delivered by uncoated or complement C3-coated mLuc/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. Data were shown as mean ± SD (n = 3).
    Figure Legend Snippet: In vivo splenic DC-specific transfection of mRNA/H 18 NPs and in vitro protein corona analysis of mRNA/H 18 NPs. (A) EGFP protein expression in splenic cell subsets of C57BL/6J mice 24 h post intravenous injection of different formulations. (B) The top 5 most abundant plasma proteins adsorbed on mRNA/H 18 NPs (C3: Complement C3; Ighm: Immunoglobulin heavy constant mu; Hbat1: Alpha-globin; Itih4: Inter alpha-trypsin inhibitor, heavy chain 4; Cnn2: Calponin). (C) Heatmap plot of major proteins in the protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. PBS group was used as a negative control. (D) Quantification of major adsorbed protein categories of different formulations. (E) Complement C3 abundance in protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. (F) Bioluminescence images of major organs and (G) Quantification of total bioluminescence flux in the spleen from C57BL/6J mice 6 h after intravenous injection of mLuc/H 18 NPs (mLuc dose of 0.25 mg kg −1 ). Mice were pre-treated with cobra venom factor (CVF) or PBS. (H) Fluorescence quantification of Cy5 mRNA delivered by uncoated or complement C3-coated Cy5-mRNA/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. (I) Bioluminescence intensity of luciferase protein translated from mRNA delivered by uncoated or complement C3-coated mLuc/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. Data were shown as mean ± SD (n = 3).

    Techniques Used: In Vivo, Transfection, In Vitro, Expressing, Injection, Clinical Proteomics, Negative Control, Combined Bisulfite Restriction Analysis Assay, Fluorescence, Incubation, Blocking Assay, Luciferase

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    Recombinant:

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    Co-Immunoprecipitation Assay:

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    Western Blot:

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    Bicinchoninic Acid Protein Assay:

    Article Title: Complement C3a promotes the formation of osteoclasts by inhibiting Sirt1 to activate the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma
    Article Snippet: .. Reagents Company Catalog no. DMEM Gibco, USA 11965092 Fetal Bovine Serum (FBS) Gibco, USA 10100157c Penicillin/Streptomycin Gibco, USA 15070-063 Phosphate Buffered Saline (PBS) Gibco, USA 20012027 Human RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-093-988 Mouse RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-094-076 Human M-CSF Miltenyi Biotec B.V. & Co. KG 130-093-963 Mouse M-CSF Miltenyi Biotec B.V. & Co. KG 130-094-129 Recombinant Mouse C3a Protein Absin abs04528 Complement C3/C3a , Mouse MedChemExpress P01027 Complement C3/C3a , Human MedChemExpress P01024 SRT1720 MedChemExpress HY-10532 SRT1720 TragetMOI T5096 Selisistat (Synonyms: EX-527) MedChemExpress HY-15452 Human/Rat Osteocalcin Antibody R&D System, Abingdon, USA MAB1419 APC Mouse Anti-Human CD34 BD Biosciences 555824 FITC Mouse Anti-Human CD16 BD Biosciences 335035 PerCP Mouse Anti-Human CD14 BD Biosciences 340660 Rabbit Anti-C3a for Co-IP Abcam ab171080 Rabbit Anti-Sirt1 for WB Cell Signaling Technology, USA # 2493S Rabbit Anti-Sirt1 for Co-IP Abcam ab189494 Rabbit Anti-PI3K for WB Cell Signaling Technology, USA # 4249S Rabbit Anti-PDK1 for WB Cell Signaling Technology, USA # 3062S Rabbit Anti-SGK3 for WB Cell Signaling Technology, USA # 8156S Rabbit Anti-P-SGK for WB ThermoFisher, USA 44-1260G Rabbit Anti-GAPDH for WB Cell Signaling Technology, USA #5174 PVDF membranes Millipore, USA IPVH00010 Enhanced BCA Protein Assay Kit Beyotime, China P0010 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio CA1210 .. DMEM Gibco, USA 11965092 Fetal Bovine Serum (FBS) Gibco, USA 10100157c Penicillin/Streptomycin Gibco, USA 15070-063 Phosphate Buffered Saline (PBS) Gibco, USA 20012027 Human RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-093-988 Mouse RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-094-076 Human M-CSF Miltenyi Biotec B.V. & Co. KG 130-093-963 Mouse M-CSF Miltenyi Biotec B.V. & Co. KG 130-094-129 Recombinant Mouse C3a Protein Absin abs04528 Complement C3/C3a , Mouse MedChemExpress P01027 Complement C3/C3a , Human MedChemExpress P01024 SRT1720 MedChemExpress HY-10532 SRT1720 TragetMOI T5096 Selisistat (Synonyms: EX-527) MedChemExpress HY-15452 Human/Rat Osteocalcin Antibody R&D System, Abingdon, USA MAB1419 APC Mouse Anti-Human CD34 BD Biosciences 555824 FITC Mouse Anti-Human CD16 BD Biosciences 335035 PerCP Mouse Anti-Human CD14 BD Biosciences 340660 Rabbit Anti-C3a for Co-IP Abcam ab171080 Rabbit Anti-Sirt1 for WB Cell Signaling Technology, USA # 2493S Rabbit Anti-Sirt1 for Co-IP Abcam ab189494 Rabbit Anti-PI3K for WB Cell Signaling Technology, USA # 4249S Rabbit Anti-PDK1 for WB Cell Signaling Technology, USA # 3062S Rabbit Anti-SGK3 for WB Cell Signaling Technology, USA # 8156S Rabbit Anti-P-SGK for WB ThermoFisher, USA 44-1260G Rabbit Anti-GAPDH for WB Cell Signaling Technology, USA #5174 PVDF membranes Millipore, USA IPVH00010 Enhanced BCA Protein Assay Kit Beyotime, China P0010 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio CA1210 RIPA buffer(high) Solarbio R0010 PMSF Solarbio P0100 Protein Phosphatase Inhibitor Solarbio p1260 SurePAGETM, Bis-Tris, 4-20%, 12 wells GenScript M00656 Goat Anti-Rabbit IgG Abcam ab6702 TBST buffer 10× Biosharp BL315B ECL chemiluminescent substrate Biosharp BL523A Human C3a ELISA Kit Elabscience China PC091

    CCK-8 Assay:

    Article Title: Complement C3a promotes the formation of osteoclasts by inhibiting Sirt1 to activate the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma
    Article Snippet: .. Reagents Company Catalog no. DMEM Gibco, USA 11965092 Fetal Bovine Serum (FBS) Gibco, USA 10100157c Penicillin/Streptomycin Gibco, USA 15070-063 Phosphate Buffered Saline (PBS) Gibco, USA 20012027 Human RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-093-988 Mouse RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-094-076 Human M-CSF Miltenyi Biotec B.V. & Co. KG 130-093-963 Mouse M-CSF Miltenyi Biotec B.V. & Co. KG 130-094-129 Recombinant Mouse C3a Protein Absin abs04528 Complement C3/C3a , Mouse MedChemExpress P01027 Complement C3/C3a , Human MedChemExpress P01024 SRT1720 MedChemExpress HY-10532 SRT1720 TragetMOI T5096 Selisistat (Synonyms: EX-527) MedChemExpress HY-15452 Human/Rat Osteocalcin Antibody R&D System, Abingdon, USA MAB1419 APC Mouse Anti-Human CD34 BD Biosciences 555824 FITC Mouse Anti-Human CD16 BD Biosciences 335035 PerCP Mouse Anti-Human CD14 BD Biosciences 340660 Rabbit Anti-C3a for Co-IP Abcam ab171080 Rabbit Anti-Sirt1 for WB Cell Signaling Technology, USA # 2493S Rabbit Anti-Sirt1 for Co-IP Abcam ab189494 Rabbit Anti-PI3K for WB Cell Signaling Technology, USA # 4249S Rabbit Anti-PDK1 for WB Cell Signaling Technology, USA # 3062S Rabbit Anti-SGK3 for WB Cell Signaling Technology, USA # 8156S Rabbit Anti-P-SGK for WB ThermoFisher, USA 44-1260G Rabbit Anti-GAPDH for WB Cell Signaling Technology, USA #5174 PVDF membranes Millipore, USA IPVH00010 Enhanced BCA Protein Assay Kit Beyotime, China P0010 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio CA1210 .. DMEM Gibco, USA 11965092 Fetal Bovine Serum (FBS) Gibco, USA 10100157c Penicillin/Streptomycin Gibco, USA 15070-063 Phosphate Buffered Saline (PBS) Gibco, USA 20012027 Human RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-093-988 Mouse RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-094-076 Human M-CSF Miltenyi Biotec B.V. & Co. KG 130-093-963 Mouse M-CSF Miltenyi Biotec B.V. & Co. KG 130-094-129 Recombinant Mouse C3a Protein Absin abs04528 Complement C3/C3a , Mouse MedChemExpress P01027 Complement C3/C3a , Human MedChemExpress P01024 SRT1720 MedChemExpress HY-10532 SRT1720 TragetMOI T5096 Selisistat (Synonyms: EX-527) MedChemExpress HY-15452 Human/Rat Osteocalcin Antibody R&D System, Abingdon, USA MAB1419 APC Mouse Anti-Human CD34 BD Biosciences 555824 FITC Mouse Anti-Human CD16 BD Biosciences 335035 PerCP Mouse Anti-Human CD14 BD Biosciences 340660 Rabbit Anti-C3a for Co-IP Abcam ab171080 Rabbit Anti-Sirt1 for WB Cell Signaling Technology, USA # 2493S Rabbit Anti-Sirt1 for Co-IP Abcam ab189494 Rabbit Anti-PI3K for WB Cell Signaling Technology, USA # 4249S Rabbit Anti-PDK1 for WB Cell Signaling Technology, USA # 3062S Rabbit Anti-SGK3 for WB Cell Signaling Technology, USA # 8156S Rabbit Anti-P-SGK for WB ThermoFisher, USA 44-1260G Rabbit Anti-GAPDH for WB Cell Signaling Technology, USA #5174 PVDF membranes Millipore, USA IPVH00010 Enhanced BCA Protein Assay Kit Beyotime, China P0010 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio CA1210 RIPA buffer(high) Solarbio R0010 PMSF Solarbio P0100 Protein Phosphatase Inhibitor Solarbio p1260 SurePAGETM, Bis-Tris, 4-20%, 12 wells GenScript M00656 Goat Anti-Rabbit IgG Abcam ab6702 TBST buffer 10× Biosharp BL315B ECL chemiluminescent substrate Biosharp BL523A Human C3a ELISA Kit Elabscience China PC091

    Cytotoxicity Assay:

    Article Title: Complement C3a promotes the formation of osteoclasts by inhibiting Sirt1 to activate the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma
    Article Snippet: .. Reagents Company Catalog no. DMEM Gibco, USA 11965092 Fetal Bovine Serum (FBS) Gibco, USA 10100157c Penicillin/Streptomycin Gibco, USA 15070-063 Phosphate Buffered Saline (PBS) Gibco, USA 20012027 Human RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-093-988 Mouse RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-094-076 Human M-CSF Miltenyi Biotec B.V. & Co. KG 130-093-963 Mouse M-CSF Miltenyi Biotec B.V. & Co. KG 130-094-129 Recombinant Mouse C3a Protein Absin abs04528 Complement C3/C3a , Mouse MedChemExpress P01027 Complement C3/C3a , Human MedChemExpress P01024 SRT1720 MedChemExpress HY-10532 SRT1720 TragetMOI T5096 Selisistat (Synonyms: EX-527) MedChemExpress HY-15452 Human/Rat Osteocalcin Antibody R&D System, Abingdon, USA MAB1419 APC Mouse Anti-Human CD34 BD Biosciences 555824 FITC Mouse Anti-Human CD16 BD Biosciences 335035 PerCP Mouse Anti-Human CD14 BD Biosciences 340660 Rabbit Anti-C3a for Co-IP Abcam ab171080 Rabbit Anti-Sirt1 for WB Cell Signaling Technology, USA # 2493S Rabbit Anti-Sirt1 for Co-IP Abcam ab189494 Rabbit Anti-PI3K for WB Cell Signaling Technology, USA # 4249S Rabbit Anti-PDK1 for WB Cell Signaling Technology, USA # 3062S Rabbit Anti-SGK3 for WB Cell Signaling Technology, USA # 8156S Rabbit Anti-P-SGK for WB ThermoFisher, USA 44-1260G Rabbit Anti-GAPDH for WB Cell Signaling Technology, USA #5174 PVDF membranes Millipore, USA IPVH00010 Enhanced BCA Protein Assay Kit Beyotime, China P0010 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio CA1210 .. DMEM Gibco, USA 11965092 Fetal Bovine Serum (FBS) Gibco, USA 10100157c Penicillin/Streptomycin Gibco, USA 15070-063 Phosphate Buffered Saline (PBS) Gibco, USA 20012027 Human RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-093-988 Mouse RANK-Ligand Miltenyi Biotec B.V. & Co. KG 130-094-076 Human M-CSF Miltenyi Biotec B.V. & Co. KG 130-093-963 Mouse M-CSF Miltenyi Biotec B.V. & Co. KG 130-094-129 Recombinant Mouse C3a Protein Absin abs04528 Complement C3/C3a , Mouse MedChemExpress P01027 Complement C3/C3a , Human MedChemExpress P01024 SRT1720 MedChemExpress HY-10532 SRT1720 TragetMOI T5096 Selisistat (Synonyms: EX-527) MedChemExpress HY-15452 Human/Rat Osteocalcin Antibody R&D System, Abingdon, USA MAB1419 APC Mouse Anti-Human CD34 BD Biosciences 555824 FITC Mouse Anti-Human CD16 BD Biosciences 335035 PerCP Mouse Anti-Human CD14 BD Biosciences 340660 Rabbit Anti-C3a for Co-IP Abcam ab171080 Rabbit Anti-Sirt1 for WB Cell Signaling Technology, USA # 2493S Rabbit Anti-Sirt1 for Co-IP Abcam ab189494 Rabbit Anti-PI3K for WB Cell Signaling Technology, USA # 4249S Rabbit Anti-PDK1 for WB Cell Signaling Technology, USA # 3062S Rabbit Anti-SGK3 for WB Cell Signaling Technology, USA # 8156S Rabbit Anti-P-SGK for WB ThermoFisher, USA 44-1260G Rabbit Anti-GAPDH for WB Cell Signaling Technology, USA #5174 PVDF membranes Millipore, USA IPVH00010 Enhanced BCA Protein Assay Kit Beyotime, China P0010 CCK-8 Cell Proliferation and Cytotoxicity Assay Kit Solarbio CA1210 RIPA buffer(high) Solarbio R0010 PMSF Solarbio P0100 Protein Phosphatase Inhibitor Solarbio p1260 SurePAGETM, Bis-Tris, 4-20%, 12 wells GenScript M00656 Goat Anti-Rabbit IgG Abcam ab6702 TBST buffer 10× Biosharp BL315B ECL chemiluminescent substrate Biosharp BL523A Human C3a ELISA Kit Elabscience China PC091

    other:

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy.
    Article Snippet: Mouse IL-4 ELISA Kit, Mouse IFN-γ ELISA Kit, Mouse IL-12 ELISA kit, Mouse IL-1β ELISA kit, Mouse IL-6 ELISA kit and Mouse TNF-α ELISA Kit were purchased from Multisciences Biotech (Hangzhou, China).



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    Image Search Results


    Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of complement C3 proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of complement C3 proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.

    Article Snippet: Mouse Complement C3 was purchased from MedChemExpress.

    Techniques: In Vivo, Injection, Adsorption, Transfection, Immunopeptidomics

    In vivo splenic DC-specific transfection of mRNA/H 18 NPs and in vitro protein corona analysis of mRNA/H 18 NPs. (A) EGFP protein expression in splenic cell subsets of C57BL/6J mice 24 h post intravenous injection of different formulations. (B) The top 5 most abundant plasma proteins adsorbed on mRNA/H 18 NPs (C3: Complement C3; Ighm: Immunoglobulin heavy constant mu; Hbat1: Alpha-globin; Itih4: Inter alpha-trypsin inhibitor, heavy chain 4; Cnn2: Calponin). (C) Heatmap plot of major proteins in the protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. PBS group was used as a negative control. (D) Quantification of major adsorbed protein categories of different formulations. (E) Complement C3 abundance in protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. (F) Bioluminescence images of major organs and (G) Quantification of total bioluminescence flux in the spleen from C57BL/6J mice 6 h after intravenous injection of mLuc/H 18 NPs (mLuc dose of 0.25 mg kg −1 ). Mice were pre-treated with cobra venom factor (CVF) or PBS. (H) Fluorescence quantification of Cy5 mRNA delivered by uncoated or complement C3-coated Cy5-mRNA/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. (I) Bioluminescence intensity of luciferase protein translated from mRNA delivered by uncoated or complement C3-coated mLuc/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. Data were shown as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: In vivo splenic DC-specific transfection of mRNA/H 18 NPs and in vitro protein corona analysis of mRNA/H 18 NPs. (A) EGFP protein expression in splenic cell subsets of C57BL/6J mice 24 h post intravenous injection of different formulations. (B) The top 5 most abundant plasma proteins adsorbed on mRNA/H 18 NPs (C3: Complement C3; Ighm: Immunoglobulin heavy constant mu; Hbat1: Alpha-globin; Itih4: Inter alpha-trypsin inhibitor, heavy chain 4; Cnn2: Calponin). (C) Heatmap plot of major proteins in the protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. PBS group was used as a negative control. (D) Quantification of major adsorbed protein categories of different formulations. (E) Complement C3 abundance in protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. (F) Bioluminescence images of major organs and (G) Quantification of total bioluminescence flux in the spleen from C57BL/6J mice 6 h after intravenous injection of mLuc/H 18 NPs (mLuc dose of 0.25 mg kg −1 ). Mice were pre-treated with cobra venom factor (CVF) or PBS. (H) Fluorescence quantification of Cy5 mRNA delivered by uncoated or complement C3-coated Cy5-mRNA/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. (I) Bioluminescence intensity of luciferase protein translated from mRNA delivered by uncoated or complement C3-coated mLuc/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. Data were shown as mean ± SD (n = 3).

    Article Snippet: Mouse Complement C3 was purchased from MedChemExpress.

    Techniques: In Vivo, Transfection, In Vitro, Expressing, Injection, Clinical Proteomics, Negative Control, Combined Bisulfite Restriction Analysis Assay, Fluorescence, Incubation, Blocking Assay, Luciferase