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MetaSystems inc cometscan image analysis software
Cometscan Image Analysis Software, supplied by MetaSystems inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cometscan+analysis+software/comet+analysis+software/pmc06555940-217-9-13
Average 90 stars, based on 1 article reviews
cometscan image analysis software - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Co-exposure effects of lead and TiO 2 nanoparticles in primary kidney cell culture from the freshwater fish Hoplias malabaricus.
Article Snippet: This study evaluated the effects of Lead (Pb) and titanium dioxide nanoparticles (TiO2 NPs) alone or in combination in anterior kidney macrophages of the freshwater fish Hoplias malabaricus, naïve or stimulated with 1 ng.mL-1 lipopolysaccharide (LPS).. Pb (1 ×10-5 to 1 ×10-1 mg.mL-1) or TiO2 NPs (1.5 ×10-6 to 1.5 ×10-2 mg.mL-1) reduced cell viability despite LPS stimulation, especially Pb 10-1 mg.mL-1.. In combination, lower concentrations of NPs intensified Pb-induced cell viability reduction while higher concentrations restored the cell viability independently of LPS stimulation.

Software:

Article Title: Exposure to arsenic in utero is associated with various types of DNA damage and micronuclei in newborns: a birth cohort study
Article Snippet: A total of 50 cells from each of the duplicate slides were examined randomly under an epi-fluorescence microscope (Axio Imager Z2, Zeiss, Germany). .. The extent of DNA damage was measured quantitatively using CometScan image analysis software (MetaSystems) and expressed as Tail length, Olive tail moment and %DNA in tail. ..

Article Title: No Effect of Caloric Restriction or Exercise on Radiation Repair Capacity
Article Snippet: .. Automated analysis of the comet assay was performed by use of a Metacyte CometScan System based on the slide scanning platform Metafer (Metasystems, Altlussheim, FRG), which consists of a motorized microscope (AxioImager M1, Carl Zeiss, Jena, Germany) with fluorescence illumination, a motorized X/Y scanning stage (Maerzhaeuser, Wetzler, Germany) with a CCD camera (CVM4+CL, JAIAS, Glostrup/Copenhagen, Denmark) and a Windows compatible PC running the Metafer software. ..

Article Title: Determination of global DNA methylation level by methylation-sensitive comet assay in patients with urinary bladder cancer.
Article Snippet: DNA methylation is an important mechanism in the regulation of gene expression and maintenance of genomic integrity.. Aberrant DNA methylation is an early event in carcinogenesis.. DNA methyltransferase inhibitors are used to restore aberrant DNA methylation and inhibit tumor growth.

Article Title: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells.
Article Snippet: .. 76 .1 - 6 /2 1/ 20 15 3 :4 3: 16 A M Gurung et al.: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells m M Tris-HCL, pH 7.4), dehydrated, dried and stained with SYBR Green dye (Trevigen) and scored with Comet Analysis Software (Metasystems, Germany). .. The images were captured using Zeiss Axioplan 2 imaging fluorescence microscope (Carl Zeiss, Germany) equipped with a triple-band filter.

Article Title: Removal of RTF2 from stalled replisomes promotes maintenance of genome integrity
Article Snippet: .. Dried slides were stained with a 1:30000 dilution of SYBR-GOLD and visualized and analyzed by Metasystems CometScan software. .. SILAC labeling: Cells were grown in DMEM F12 medium for SILAC (Pierce) supplemented with 10% (v/v) dialyzed FBS (Pierce), glutamax and 100 units of penicillin/streptomycin per ml (Invitrogen).

Article Title: Differential resistance of human embryonic stem cells and somatic cell types to hydrogen peroxide-induced genotoxicity may be dependent on innate basal intracellular ROS levels
Article Snippet: The slides were dried and then stained with SYBR green dye (Molecular Probes, Eugene, OR, USA) prior to being observed under a Zeiss Axioplan 2 imaging fluorescence microscope (Carl Zeiss, Wetzlar, Germany) equipped with triple band filter. .. Images were captured using Comet Analysis Software (Metasystems, Altlussheim, Germany). ..

Single Cell Gel Electrophoresis:

Article Title: No Effect of Caloric Restriction or Exercise on Radiation Repair Capacity
Article Snippet: .. Automated analysis of the comet assay was performed by use of a Metacyte CometScan System based on the slide scanning platform Metafer (Metasystems, Altlussheim, FRG), which consists of a motorized microscope (AxioImager M1, Carl Zeiss, Jena, Germany) with fluorescence illumination, a motorized X/Y scanning stage (Maerzhaeuser, Wetzler, Germany) with a CCD camera (CVM4+CL, JAIAS, Glostrup/Copenhagen, Denmark) and a Windows compatible PC running the Metafer software. ..

Article Title: Determination of global DNA methylation level by methylation-sensitive comet assay in patients with urinary bladder cancer.
Article Snippet: DNA methylation is an important mechanism in the regulation of gene expression and maintenance of genomic integrity.. Aberrant DNA methylation is an early event in carcinogenesis.. DNA methyltransferase inhibitors are used to restore aberrant DNA methylation and inhibit tumor growth.

Microscopy:

Article Title: No Effect of Caloric Restriction or Exercise on Radiation Repair Capacity
Article Snippet: .. Automated analysis of the comet assay was performed by use of a Metacyte CometScan System based on the slide scanning platform Metafer (Metasystems, Altlussheim, FRG), which consists of a motorized microscope (AxioImager M1, Carl Zeiss, Jena, Germany) with fluorescence illumination, a motorized X/Y scanning stage (Maerzhaeuser, Wetzler, Germany) with a CCD camera (CVM4+CL, JAIAS, Glostrup/Copenhagen, Denmark) and a Windows compatible PC running the Metafer software. ..

Fluorescence:

Article Title: No Effect of Caloric Restriction or Exercise on Radiation Repair Capacity
Article Snippet: .. Automated analysis of the comet assay was performed by use of a Metacyte CometScan System based on the slide scanning platform Metafer (Metasystems, Altlussheim, FRG), which consists of a motorized microscope (AxioImager M1, Carl Zeiss, Jena, Germany) with fluorescence illumination, a motorized X/Y scanning stage (Maerzhaeuser, Wetzler, Germany) with a CCD camera (CVM4+CL, JAIAS, Glostrup/Copenhagen, Denmark) and a Windows compatible PC running the Metafer software. ..

Gene Expression:

Article Title: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells.
Article Snippet: .. 76 .1 - 6 /2 1/ 20 15 3 :4 3: 16 A M Gurung et al.: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells m M Tris-HCL, pH 7.4), dehydrated, dried and stained with SYBR Green dye (Trevigen) and scored with Comet Analysis Software (Metasystems, Germany). .. The images were captured using Zeiss Axioplan 2 imaging fluorescence microscope (Carl Zeiss, Germany) equipped with a triple-band filter.

Staining:

Article Title: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells.
Article Snippet: .. 76 .1 - 6 /2 1/ 20 15 3 :4 3: 16 A M Gurung et al.: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells m M Tris-HCL, pH 7.4), dehydrated, dried and stained with SYBR Green dye (Trevigen) and scored with Comet Analysis Software (Metasystems, Germany). .. The images were captured using Zeiss Axioplan 2 imaging fluorescence microscope (Carl Zeiss, Germany) equipped with a triple-band filter.

Article Title: Removal of RTF2 from stalled replisomes promotes maintenance of genome integrity
Article Snippet: .. Dried slides were stained with a 1:30000 dilution of SYBR-GOLD and visualized and analyzed by Metasystems CometScan software. .. SILAC labeling: Cells were grown in DMEM F12 medium for SILAC (Pierce) supplemented with 10% (v/v) dialyzed FBS (Pierce), glutamax and 100 units of penicillin/streptomycin per ml (Invitrogen).

SYBR Green Assay:

Article Title: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells.
Article Snippet: .. 76 .1 - 6 /2 1/ 20 15 3 :4 3: 16 A M Gurung et al.: MST-312 Alters Telomere Dynamics, Gene Expression Profiles and Growth in Human Breast Cancer Cells m M Tris-HCL, pH 7.4), dehydrated, dried and stained with SYBR Green dye (Trevigen) and scored with Comet Analysis Software (Metasystems, Germany). .. The images were captured using Zeiss Axioplan 2 imaging fluorescence microscope (Carl Zeiss, Germany) equipped with a triple-band filter.

Electrophoresis:

Article Title: Mediated effect of ultrasound treated Diclofenac on mussel hemocytes: First evidence for the involvement of respiratory burst enzymes in the induction of DCF-mediated unspecific mode of action.
Article Snippet: The present study investigates the toxic behavior of diclofenac (DCF) before and after its ultrasound (US) treatment, as well as the involvement of intracellular target molecules, such as NADPH oxidase and NO synthase, in the DCF-induced adverse effects on hemocytes of mussel Mytilus galloprovincialis.. In this context, appropriate volumes (350 and 500 mL) of DCF solutions (at concentrations of 2, 2.5, 5 and 10 mg L) were treated under different ultrasound operating conditions (frequency at 582 and 862 kHz, electric power density at 133 and 167 W) for assessing US method efficiency.. In parallel, DCF and US DCF-mediated cytotoxic (in terms of cell viability measured with the use of neutral red uptake/NRU method), oxidative (in terms of superoxide anions/O2, nitric oxides such as NO2 and lipid peroxidation products, such as malondialdehyde/MDA content) and genotoxic (DNA damage measured by the use of Comet assay method) effects were investigated in hemocytes exposed for 1h to 5, 10 and 100 ng L and 1, 10 and 20 μg L of DCF.



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