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anti phospho cjun  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho cjun
    Anti Phospho Cjun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cjun/pm41579710-74-36-42
    Average 86 stars, based on 1 article reviews
    anti phospho cjun - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria.
    Article Snippet: The following primary antibodies were used for IHC and WBs: anti–βIII-tubulin (rabbit, IHC, 1:1,000; PRB-435P; Covance), anti– βIII-tubulin (mouse, IHC, 1:1,000; MMS-435P; Covance), anti-caspr, (rabbit, IHC, 1:1,000, provided by E. Peles, Weizmann Institute, Rehovot, Israel), anti–pan Na+ channels (mouse, IHC, 1:1,000; S8809; Sigma-Aldrich); anti-GAP DH (rabbit, WB, 1:1,000; 14C10; Cell Signaling Technology), anti-ERK (rabbit, WB, 1:10,000; K-23; Santa Cruz Biotechnology), anti–phospho-ERK (rabbit, WB, 1:1,000; 9101; Cell Signaling Technology), anti-TrkA (rabbit, WB, 1:500; 2505; Cell Signaling Technology), anti–phospho-TrkA Tyr674/675 (rabbit, WB, 1:500; 4621; Cell Signaling Technology), anti-JNK (rabbit, WB, 1:500; 9251; Cell Signaling Technology), anti-Thr183/Tyr185 phospho-JNK (rabbit, WB, 1:500; 4668; Cell Signaling Technology), anti– cytochrome c (mouse, ICC, 1:550; 612302; BioLegend); anti-MKK4 (rabbit, WB, 1:1,000; 9152; Cell Signaling Technology); anti-Ser257/ Thr261 phospho-MKK4 (rabbit, WB, 1:1,000; 9156; Cell Signaling Technology), anti-Ser63 phospho-c-Jun (rabbit, WB, 1:100; 9164; Cell Signaling Technology), anti-Bax (rabbit, WB, 1:500; 2772; Cell Signaling Technology), anti-(active) BAX 6A7 (mouse, WB, 1:100; B8429; Sigma-Aldrich), anti–spectrin αII chain (nonerythroid; mouse, WB, 1:500; MAB1622; EMD Millipore), anti –α-tubulin (mouse, WB, 1:1,000; T5168; Sigma-Aldrich), anti–cleaved caspase-3 (rabbit, WB, 1:100; IHC, 1:100; AB3623; EMD Millipore), anti-Bim/Bod (rabbit, WB, 1:1,000; ADI-AAP-330-E; Enzo Life Sciences), anti–neurofilament L (rabbit, WB, 1:1,000; 2837; Cell Signaling), anti –α-internexin (rabbit, 1:1,000; AB5354; Chemicon), anti-SCG10 (rabbit, 1:1,000; 10586-1-AP; Proteintech Group), and anti-Opa1 (rabbit, 1:750; 67589; Cell Signaling Technology).

    Modification:

    Article Title: Cirsium setidens Nakai extract and pectolinarin as active compounds promote innate immunity in RAW 264.7 cells and splenocytes
    Article Snippet: .. Dulbecco’s modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI)-1640 medium, penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from HyClone (Logan, UT, USA). p- ERK 1/2 (#4370), ERK 1/2 (#9102), p-JNK 1/2 (#4688), JNK 1/2 (#9252), p-p38 (#4511), p38 (#9212), p-c-Jun (#9164), c-Jun (#9165), p-p65 (#3033), p65 (#8242), p-IKKα/β (#2697), IKKα (#61,294), p-IκBα (#2859), IκBα (#4814), iNOS (#13,120), COX-2 (#12,282), and α/β tubulin (#2148) were procured from Cell Signaling Technologies (Danvers, MA, USA). .. The antibody specific for lamin B1 (ab16048) was acquired from Abcam (Cambridge, Cambridgeshire, UK), and that specific for β-actin (sc-47,778) was purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

    Synthesized:

    Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline
    Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), p–c-JUN (Ser63) (Cell Signaling, #9164, 1:1000), c-JUN (Cell Signaling, #9165, 1:1000), FOSL1 (Cell Signaling, #5281, 1:1000), CEBPB (Cell Signaling, #3087, 1:1000), and GAPDH (Cell Signaling, #2118, 1:5000). qRT‐PCR RNA was extracted using TRIzol (Invitrogen, #15,596,026). cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad, #1,708,891). qPCR was performed using SYBR Green Master Mix (Applied Biosystems, #A25742). ..

    cDNA Synthesis:

    Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline
    Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), p–c-JUN (Ser63) (Cell Signaling, #9164, 1:1000), c-JUN (Cell Signaling, #9165, 1:1000), FOSL1 (Cell Signaling, #5281, 1:1000), CEBPB (Cell Signaling, #3087, 1:1000), and GAPDH (Cell Signaling, #2118, 1:5000). qRT‐PCR RNA was extracted using TRIzol (Invitrogen, #15,596,026). cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad, #1,708,891). qPCR was performed using SYBR Green Master Mix (Applied Biosystems, #A25742). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline
    Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), p–c-JUN (Ser63) (Cell Signaling, #9164, 1:1000), c-JUN (Cell Signaling, #9165, 1:1000), FOSL1 (Cell Signaling, #5281, 1:1000), CEBPB (Cell Signaling, #3087, 1:1000), and GAPDH (Cell Signaling, #2118, 1:5000). qRT‐PCR RNA was extracted using TRIzol (Invitrogen, #15,596,026). cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad, #1,708,891). qPCR was performed using SYBR Green Master Mix (Applied Biosystems, #A25742). ..

    SYBR Green Assay:

    Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline
    Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), p–c-JUN (Ser63) (Cell Signaling, #9164, 1:1000), c-JUN (Cell Signaling, #9165, 1:1000), FOSL1 (Cell Signaling, #5281, 1:1000), CEBPB (Cell Signaling, #3087, 1:1000), and GAPDH (Cell Signaling, #2118, 1:5000). qRT‐PCR RNA was extracted using TRIzol (Invitrogen, #15,596,026). cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad, #1,708,891). qPCR was performed using SYBR Green Master Mix (Applied Biosystems, #A25742). ..

    Blocking Assay:

    Article Title: The inhibition of Il1β synthesis mediated by a novel pyridine-sulfonamide compound protects against the progression of metabolic dysfunction-associated steatotic liver disease.
    Article Snippet: .. After blocking, membranes were incubated overnight with primary antibodies at 4◦C: anti-IκBα (sc-371) and anti-JNK (sc7345) from Santa Cruz Biotechnology Inc.; anti-IL1β (AF-401-NA) from Bio-Techne R&D Systems; anti-cleaved caspase-3 (#9661), antiphospho-JNK (#4668), anti-phospho-p38 (#9211), anti-p38 (#9212), anti-phospho-cJun (#9164) and anti-cJun (#9165) from Cell Signaling Technology. .. Finally, membranes were incubated with the corresponding secondary antibody (Santa Cruz Biotechnology Inc.).

    Incubation:

    Article Title: The inhibition of Il1β synthesis mediated by a novel pyridine-sulfonamide compound protects against the progression of metabolic dysfunction-associated steatotic liver disease.
    Article Snippet: .. After blocking, membranes were incubated overnight with primary antibodies at 4◦C: anti-IκBα (sc-371) and anti-JNK (sc7345) from Santa Cruz Biotechnology Inc.; anti-IL1β (AF-401-NA) from Bio-Techne R&D Systems; anti-cleaved caspase-3 (#9661), antiphospho-JNK (#4668), anti-phospho-p38 (#9211), anti-p38 (#9212), anti-phospho-cJun (#9164) and anti-cJun (#9165) from Cell Signaling Technology. .. Finally, membranes were incubated with the corresponding secondary antibody (Santa Cruz Biotechnology Inc.).

    Recombinant:

    Article Title: RAC1P29S Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies phospho-AKT (T308) CST #13038 phospho-AKT (S473) CST #9271 phospho-AKT (S473) CST #4060 AKT CST #2920 phospho-ERK1/2 (T202/T204) CST #9101 ERK1/2 CST #9107 phospho-PAK1/2 (S199/192) CST #2605 phospho-PAK1/2 (T423/402) CST #2601 PAK1/2/3 CST #2604 phospho-MEK1/2 (S217/221) CST #9145 RAC1 (23A8) Merck Millipore 05-389 Vinculin (hVIN-1) Sigma-Aldrich V9131 Myc (9E10) In-house (CRUK LRI) N/A Vimentin (EPR3776) Abcam ab92547 N-cadherin CST #13116 c-Jun CST #9165 phospho-c-Jun (S73) CST #9164 ER (MC-20) Santa Cruz sc-542 Fibronectin Abcam ab2413 phospho-BRAF (S445) CST #2696 phospho-CRAF (S338) CST #9427 S100 (4C4.9) Abcam ab4066 SOX10 (A2) Santa Cruz sc-365692 p16 (EPR1473) Abcam ab108349 p27 (F-8) Santa Cruz sc-1641 B220 (RA3-6B2) BD Biosciences 553086 B220-BV510 Biolegend RA3-6B2 CD19-BV605 Biolegend 6D5 CD38-APC Biolegend 90 CD95-BV421 BD Biosciences Jo2 CD138-BV786 BD Biosciences 281-2 NK1.1-PE Biolegend PK136 CD44-APC Ebioscience IM7 CD62L-PeCy7 Ebioscience MEL-14 CD3e-PerCPEFluor710 Ebioscience 145-2C11 CD4-BV421 Biolegend GK1.5 CD8a-FITC Ebioscience 53-6.7 PTEN CST #9559 Chemicals, Peptides, and Recombinant Proteins 4OH-tamoxifen Sigma-Aldrich H6278 Tamoxifen Sigma-Aldrich T5648 CCG-1423 Selleckchem S7719 CCG-203971 Tocris 5277 (Continued on next page) e1 Cancer Cell 36, 68–83.e1–e9, July 8, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER CCG-257081 In-house (MSU) N/A G-5555 Medchem Express HY-19635 EHT-1864 Tocris 3872 Latrunculin A Tocris 3973 JNK-IN-8 Selleckchem S4901 EHop-016 Selleckchem S7319 CK-666 Sigma-Aldrich SML0006 MK-2206 Selleckchem S1078 CK-869 Sigma-Aldrich C9124 Saracatinib Cambridge Bioscience CAY11497 LGK-974 Selleckchem S7143 Avagacestat Selleckchem S1262 NSC-23766 Tocris 2161 (5Z)-7-Oxozeaenol Tocris 3604 SP-600125 Selleckchem S1460 PF-562271 Abcam ab141360 Obatoclax Selleckchem S1057 Navitoclax (ABT263) Selleckchem S1001 Topotecan Axxora 4100 Etoposide Calbiochem 341205 PLX4720 Plexxicon N.A.



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    Cell Signaling Technology Inc p cjun
    A subpopulation of RGCs express the stress marker phosphorylated c-JUN <t>(p-cJUN).</t> (A) Whole mount image ( Cx3cr1-Gfp/+ ) of e14.5 retina showing that a subset of RGCs (Brn3a+; red) express nuclear p-cJUN (white; yellow arrows). Boxed regions show enlarged region and label for p-cJUN alone or Brn3a alone (A′) . Scale bar = 10 μm. (B) Whole mount image (e14.5 Cx3cr1-Gfp/+ ) showing microglia (GFP) internalizing an RGC (Brn3a + red) labeled with p-cJUN (white) using HCR IHC. Scale bar = 1 μm. (C) Whole mount images showing RGCs (Brn3a+; red) with nuclear p-cJUN (white) in control mice (WT) and Mertk/CR3 dKO mice. Yellow arrows indicate double positive p-cJUN and Brn3a staining. (D) Quantification of double positive p-cJUN + Brn3a + RGCs per mm 2 in e14.5 control and Mertk/CR3 dKO mice. *** p < 0.01 unpaired t -test [ N = 4 (WT), 5 (Mertk CR3 dKO)]. (E) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in e14.5 and e16.5 Mertk/CR3 dKO mice. * p < 0.05 Welch’s t -test [ N = 5 (e14.5), 4 (e16.5)]. (F) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in P0 control, Mertk KO and Mertk/CR3 dKO mice. ns, not significant. Welch’s one-way ANOVA with Brown–Forsythe variance test [ N = 7 (WT), 7 (Mertk KO), 6 (Mertk CR3 dKO)]. (G) Whole mount retina images to show vehicle treated Axl KO mice with RGCs (Brn3a+; red) expressing nuclear p-cJUN (white) and microglia present (IBA1+; green). Scale bar = 500 μm. Boxed regions show enlarged area (G′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100-10 μm. (H) Whole mount retina images to show Axl KO PLX treated mice with depletion of microglia (IBA1; green) and RGCs (Brn3a+; red) with nuclear p-cJUN (white) expression remaining. Boxed regions show enlarged area (H′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100 to 10 μm.
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    Image Search Results


    A subpopulation of RGCs express the stress marker phosphorylated c-JUN (p-cJUN). (A) Whole mount image ( Cx3cr1-Gfp/+ ) of e14.5 retina showing that a subset of RGCs (Brn3a+; red) express nuclear p-cJUN (white; yellow arrows). Boxed regions show enlarged region and label for p-cJUN alone or Brn3a alone (A′) . Scale bar = 10 μm. (B) Whole mount image (e14.5 Cx3cr1-Gfp/+ ) showing microglia (GFP) internalizing an RGC (Brn3a + red) labeled with p-cJUN (white) using HCR IHC. Scale bar = 1 μm. (C) Whole mount images showing RGCs (Brn3a+; red) with nuclear p-cJUN (white) in control mice (WT) and Mertk/CR3 dKO mice. Yellow arrows indicate double positive p-cJUN and Brn3a staining. (D) Quantification of double positive p-cJUN + Brn3a + RGCs per mm 2 in e14.5 control and Mertk/CR3 dKO mice. *** p < 0.01 unpaired t -test [ N = 4 (WT), 5 (Mertk CR3 dKO)]. (E) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in e14.5 and e16.5 Mertk/CR3 dKO mice. * p < 0.05 Welch’s t -test [ N = 5 (e14.5), 4 (e16.5)]. (F) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in P0 control, Mertk KO and Mertk/CR3 dKO mice. ns, not significant. Welch’s one-way ANOVA with Brown–Forsythe variance test [ N = 7 (WT), 7 (Mertk KO), 6 (Mertk CR3 dKO)]. (G) Whole mount retina images to show vehicle treated Axl KO mice with RGCs (Brn3a+; red) expressing nuclear p-cJUN (white) and microglia present (IBA1+; green). Scale bar = 500 μm. Boxed regions show enlarged area (G′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100-10 μm. (H) Whole mount retina images to show Axl KO PLX treated mice with depletion of microglia (IBA1; green) and RGCs (Brn3a+; red) with nuclear p-cJUN (white) expression remaining. Boxed regions show enlarged area (H′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100 to 10 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Microglial mechanisms of viable retinal ganglion cell elimination

    doi: 10.3389/fncel.2025.1719791

    Figure Lengend Snippet: A subpopulation of RGCs express the stress marker phosphorylated c-JUN (p-cJUN). (A) Whole mount image ( Cx3cr1-Gfp/+ ) of e14.5 retina showing that a subset of RGCs (Brn3a+; red) express nuclear p-cJUN (white; yellow arrows). Boxed regions show enlarged region and label for p-cJUN alone or Brn3a alone (A′) . Scale bar = 10 μm. (B) Whole mount image (e14.5 Cx3cr1-Gfp/+ ) showing microglia (GFP) internalizing an RGC (Brn3a + red) labeled with p-cJUN (white) using HCR IHC. Scale bar = 1 μm. (C) Whole mount images showing RGCs (Brn3a+; red) with nuclear p-cJUN (white) in control mice (WT) and Mertk/CR3 dKO mice. Yellow arrows indicate double positive p-cJUN and Brn3a staining. (D) Quantification of double positive p-cJUN + Brn3a + RGCs per mm 2 in e14.5 control and Mertk/CR3 dKO mice. *** p < 0.01 unpaired t -test [ N = 4 (WT), 5 (Mertk CR3 dKO)]. (E) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in e14.5 and e16.5 Mertk/CR3 dKO mice. * p < 0.05 Welch’s t -test [ N = 5 (e14.5), 4 (e16.5)]. (F) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in P0 control, Mertk KO and Mertk/CR3 dKO mice. ns, not significant. Welch’s one-way ANOVA with Brown–Forsythe variance test [ N = 7 (WT), 7 (Mertk KO), 6 (Mertk CR3 dKO)]. (G) Whole mount retina images to show vehicle treated Axl KO mice with RGCs (Brn3a+; red) expressing nuclear p-cJUN (white) and microglia present (IBA1+; green). Scale bar = 500 μm. Boxed regions show enlarged area (G′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100-10 μm. (H) Whole mount retina images to show Axl KO PLX treated mice with depletion of microglia (IBA1; green) and RGCs (Brn3a+; red) with nuclear p-cJUN (white) expression remaining. Boxed regions show enlarged area (H′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100 to 10 μm.

    Article Snippet: Antibody , Rabbit anti p-cJUN , Cell Signaling , 1:500 , 9261S.

    Techniques: Marker, Labeling, Control, Staining, Expressing