anti phospho cjun (Cell Signaling Technology Inc)
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Anti Phospho Cjun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cjun/pm41579710-74-36-42
Average 86 stars, based on 1 article reviews
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other:Article Title: A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria. Article Snippet: The following primary antibodies were used for IHC and WBs: anti–βIII-tubulin (rabbit, IHC, 1:1,000; PRB-435P; Covance), anti– βIII-tubulin (mouse, IHC, 1:1,000; MMS-435P; Covance), anti-caspr, (rabbit, IHC, 1:1,000, provided by E. Peles, Weizmann Institute, Rehovot, Israel), anti–pan Na+ channels (mouse, IHC, 1:1,000; S8809; Sigma-Aldrich); Modification:Article Title: Cirsium setidens Nakai extract and pectolinarin as active compounds promote innate immunity in RAW 264.7 cells and splenocytes Article Snippet: .. Dulbecco’s modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI)-1640 medium, penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from HyClone (Logan, UT, USA). p- ERK 1/2 (#4370), ERK 1/2 (#9102), p-JNK 1/2 (#4688), JNK 1/2 (#9252), p-p38 (#4511), p38 (#9212), Synthesized:Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), cDNA Synthesis:Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), Real-time Polymerase Chain Reaction:Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), SYBR Green Assay:Article Title: Targeting a master inflammatory switch in the aging cochlea attenuates sensory decline Article Snippet: .. Primary antibodies used were: p-RELA (Ser536) (Cell Signaling, #3033, 1:1000), RELA (Cell Signaling, #8242, 1:1000), Blocking Assay:Article Title: The inhibition of Il1β synthesis mediated by a novel pyridine-sulfonamide compound protects against the progression of metabolic dysfunction-associated steatotic liver disease. Article Snippet: .. After blocking, membranes were incubated overnight with primary antibodies at 4◦C: anti-IκBα (sc-371) and anti-JNK (sc7345) from Santa Cruz Biotechnology Inc.; anti-IL1β (AF-401-NA) from Bio-Techne R&D Systems; anti-cleaved caspase-3 (#9661), antiphospho-JNK (#4668), anti-phospho-p38 (#9211), anti-p38 (#9212), Incubation:Article Title: The inhibition of Il1β synthesis mediated by a novel pyridine-sulfonamide compound protects against the progression of metabolic dysfunction-associated steatotic liver disease. Article Snippet: .. After blocking, membranes were incubated overnight with primary antibodies at 4◦C: anti-IκBα (sc-371) and anti-JNK (sc7345) from Santa Cruz Biotechnology Inc.; anti-IL1β (AF-401-NA) from Bio-Techne R&D Systems; anti-cleaved caspase-3 (#9661), antiphospho-JNK (#4668), anti-phospho-p38 (#9211), anti-p38 (#9212), Recombinant:Article Title: RAC1P29S Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies phospho-AKT (T308) CST #13038 phospho-AKT (S473) CST #9271 phospho-AKT (S473) CST #4060 AKT CST #2920 phospho-ERK1/2 (T202/T204) CST #9101 ERK1/2 CST #9107 phospho-PAK1/2 (S199/192) CST #2605 phospho-PAK1/2 (T423/402) CST #2601 PAK1/2/3 CST #2604 phospho-MEK1/2 (S217/221) CST #9145 RAC1 (23A8) Merck Millipore 05-389 Vinculin (hVIN-1) Sigma-Aldrich V9131 Myc (9E10) In-house (CRUK LRI) N/A Vimentin (EPR3776) Abcam ab92547 N-cadherin CST #13116 c-Jun CST #9165 |
![A subpopulation of RGCs express the stress marker phosphorylated c-JUN <t>(p-cJUN).</t> (A) Whole mount image ( Cx3cr1-Gfp/+ ) of e14.5 retina showing that a subset of RGCs (Brn3a+; red) express nuclear p-cJUN (white; yellow arrows). Boxed regions show enlarged region and label for p-cJUN alone or Brn3a alone (A′) . Scale bar = 10 μm. (B) Whole mount image (e14.5 Cx3cr1-Gfp/+ ) showing microglia (GFP) internalizing an RGC (Brn3a + red) labeled with p-cJUN (white) using HCR IHC. Scale bar = 1 μm. (C) Whole mount images showing RGCs (Brn3a+; red) with nuclear p-cJUN (white) in control mice (WT) and Mertk/CR3 dKO mice. Yellow arrows indicate double positive p-cJUN and Brn3a staining. (D) Quantification of double positive p-cJUN + Brn3a + RGCs per mm 2 in e14.5 control and Mertk/CR3 dKO mice. *** p < 0.01 unpaired t -test [ N = 4 (WT), 5 (Mertk CR3 dKO)]. (E) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in e14.5 and e16.5 Mertk/CR3 dKO mice. * p < 0.05 Welch’s t -test [ N = 5 (e14.5), 4 (e16.5)]. (F) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in P0 control, Mertk KO and Mertk/CR3 dKO mice. ns, not significant. Welch’s one-way ANOVA with Brown–Forsythe variance test [ N = 7 (WT), 7 (Mertk KO), 6 (Mertk CR3 dKO)]. (G) Whole mount retina images to show vehicle treated Axl KO mice with RGCs (Brn3a+; red) expressing nuclear p-cJUN (white) and microglia present (IBA1+; green). Scale bar = 500 μm. Boxed regions show enlarged area (G′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100-10 μm. (H) Whole mount retina images to show Axl KO PLX treated mice with depletion of microglia (IBA1; green) and RGCs (Brn3a+; red) with nuclear p-cJUN (white) expression remaining. Boxed regions show enlarged area (H′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100 to 10 μm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5010/pmc12715010/pmc12715010__fncel-19-1719791-g002.jpg)