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citrate repair solution  (OriGene)


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    Structured Review

    OriGene citrate repair solution
    Citrate Repair Solution, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/citrate+buffer+solution/Citrate+Buffer+pH+6%2E0/pm42007524-97-24-28
    Average 95 stars, based on 81 article reviews
    citrate repair solution - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Ketogenic diet regulates Uch-L1(C) to improve cerebral energy metabolism and cognitive function in Alzheimer's disease mice
    Article Snippet: Finally, after adding 100 μL sample or standard solution with proper solubility to the opaque white 96-well plate, the fluorescence reaction was carried out immediately and detect the fluorescence intensity by spectrophotometer (SpectraMax Paradigm; Molecular Devices, Cardiff, UK). .. In order to evaluate the morphology and apoptosis of nerve cells, the paraffin sections of mouse brain (coronal section) were dewaxed and repaired with citrate repair solution (ZLI-9065; Beijing Zhongshan Jinqiao, Beijing, China), and then sealed with goat serum for 20 min. Then, incubated with primary antibody as described in , at 4°C overnight, then incubated with the secondary antibody (AP-9001/9002; Beijing Zhongshan Jinqiao,) at 37°C for 30 min, and finally developed color with concentrated DAB kit (K135925C; Beijing Zhongshan Jinqiao). .. TdT-mediated dUTP Nick-End Labeling (TUNEL) (49330900; Roche, Basel, Switzerland) was used to detect apoptosis of nerve cells.

    Article Title: Ketogenic diet regulates Uch-L1(C) to improve cerebral energy metabolism and cognitive function in Alzheimer's disease mice.
    Article Snippet: Finally, after adding 100 μL sample or standard solution with proper solubility to the opaque white 96-well plate, the fluorescence reaction was carried out immediately and detect the fluorescence intensity by spectrophotometer (SpectraMax Paradigm; Molecular Devices, Cardiff, UK). .. In order to evaluate the morphology and apoptosis of nerve cells, the paraffin sections of mouse brain (coronal section) were dewaxed and repaired with citrate repair solution (ZLI-9065; Beijing Zhongshan Jinqiao, Beijing, China), and then sealed with goat serum for 20 min. Then, incubated with primary antibody as described in Table 1, at 4°C overnight, then incubated with the secondary antibody (AP-9001/9002; Beijing Zhongshan Jinqiao,) at 37°C for 30 min, and finally developed color with concentrated DAB kit (K135925C; Beijing Zhongshan Jinqiao). .. TdT-mediated dUTP Nick-End Labeling (TUNEL) (49330900; Roche, Basel, Switzerland) was used to detect apoptosis of nerve cells.

    Activity Assay:

    Article Title: Targeting ATF4-DDIT4/TXNIP induced mitochondrial dysfunction and ferroptosis: ISRIB as novel therapy for septic cardiomyopathy.
    Article Snippet: .. After dewaxing the paraffin sections in water, antigen retrieval was performed using a citrate buffer (Zhongshan Golden Bridge Biotechnology, Beijing, China) to inhibit endogenous peroxidase activity. ..

    Staining:

    Article Title: A progenitor cell population contributes to prenatal injury repair and neonatal antimicrobial defense in the small intestine.
    Article Snippet: .. Before staining, the sections were dried at room temperature for 30 min, then washed with PBS for 10 min to remove OCT. Heat-induced epitope retrieval was performed by incubating sections with citrate buffer (10 mM citrate, 0.05% Tween 20, pH 6.0, OriGene) in 95◦C water bath for 15–30 min. .. Following gradual cooling to room temperature, sections were permeabilized with 0.1% Triton X-100 (Merck) in PBS at 4◦C for 15 min and blocked with 3% BSA (Millipore) and 0.01% Triton X-100 in PBS for 1–2 h at room temperature.

    Article Title: Personalized prediction of chemotherapy efficacy in osteosarcoma through patient-derived organoids: correlation with survival and tumor proliferation potential
    Article Snippet: .. For IF staining, deparaffinized and rehydrated sections were subjected to heat-induced antigen retrieval in citrate buffer (pH 9.0; OriGene, #ZLI-9069; Beijing, China) via a steam pressure cooker. ..

    Article Title: Ultrasound-Triggering Carbon Monoxide Release from Rhodium Nanoparticles for Myocardial Infarction Treatment
    Article Snippet: The TUNEL apoptosis detection kit (chromogenic method) and proteinase K were purchased from Shanghai Biyuntian Biotechnology Co., Ltd. .. The peroxidase blocking solution, PBS powder, citrate buffer, secondary antibody staining kit and DAB chromogenic kit were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. .. The H9c2 cell line was obtained from ACROBiosystems Co., Ltd.

    Serial Time-encoded Amplified Microscopy:

    Article Title: Personalized prediction of chemotherapy efficacy in osteosarcoma through patient-derived organoids: correlation with survival and tumor proliferation potential
    Article Snippet: .. For IF staining, deparaffinized and rehydrated sections were subjected to heat-induced antigen retrieval in citrate buffer (pH 9.0; OriGene, #ZLI-9069; Beijing, China) via a steam pressure cooker. ..

    Blocking Assay:

    Article Title: Ultrasound-Triggering Carbon Monoxide Release from Rhodium Nanoparticles for Myocardial Infarction Treatment
    Article Snippet: The TUNEL apoptosis detection kit (chromogenic method) and proteinase K were purchased from Shanghai Biyuntian Biotechnology Co., Ltd. .. The peroxidase blocking solution, PBS powder, citrate buffer, secondary antibody staining kit and DAB chromogenic kit were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. .. The H9c2 cell line was obtained from ACROBiosystems Co., Ltd.



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