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Sangon Biotech citrate buffer solution
Citrate Buffer Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/citrate+buffer+solution/buffer+citrate+solution/pm42107063-274-7-11
Average 86 stars, based on 1 article reviews
citrate buffer solution - by Bioz Stars, 2026-09
86/100 stars

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Activity Assay:

Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
Article Snippet: .. Antigen retrieval was subsequently performed sing a citrate buffer solution (from Sangon Biotech), and ndogenous peroxidase activity was blocked at 37◦C for 30 min. hereafter, the slides were blocked for 10 min, followed by an vernight 4◦C incubation with primary antibodies, and then a h incubation at room temperature with secondary antibodies. taining was then achieved using the GTVisionTM III Detecion System/Mo&Rb (GeneTech, Shanghai, China) following the anufacturer’s protocol. .. The primary antibodies employed in the HC analysis are listed as follows: UGDH (AG3528, diluted 1:200, eyotime), p-UGDH (diluted 1:200), UXS1 (GTX120038, diluted :200, Genetex). dvanced Science, 2026 To quantify the IHC results, four random fields of view per slide were assessed for staining intensity and coverage.

Incubation:

Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
Article Snippet: .. Antigen retrieval was subsequently performed sing a citrate buffer solution (from Sangon Biotech), and ndogenous peroxidase activity was blocked at 37◦C for 30 min. hereafter, the slides were blocked for 10 min, followed by an vernight 4◦C incubation with primary antibodies, and then a h incubation at room temperature with secondary antibodies. taining was then achieved using the GTVisionTM III Detecion System/Mo&Rb (GeneTech, Shanghai, China) following the anufacturer’s protocol. .. The primary antibodies employed in the HC analysis are listed as follows: UGDH (AG3528, diluted 1:200, eyotime), p-UGDH (diluted 1:200), UXS1 (GTX120038, diluted :200, Genetex). dvanced Science, 2026 To quantify the IHC results, four random fields of view per slide were assessed for staining intensity and coverage.

Article Title: Divergent Virulence Traits in Klebsiella Pneumoniae Strains of Capsular Serotype K2 and ST25: Insights from Whole-Genome Sequencing
Article Snippet: .. The bacterial cells were then mixed with 200 μL of 1% Zwittergent (Sangon Biotech) in 100 mM citric acid buffer (Sangon Biotech) at 55°C for 30 min. After centrifugation at 10000 g for 10 min, 0.4 mL of the cell-free supernatant was combined with 1.6 mL of absolute ethanol and incubated for 30 min at 4°C. .. The samples were then centrifuged, rehydrated with 125 μL of water overnight, and mixed with 0.5 mL of 12.5 mM sodium tetraborate (Sigma) in concentrated sulfuric acid (Sinopharm).

Blocking Assay:

Article Title: Luteolin Attenuates Asthma Via Inhibiting ALOX15 Mediated Bronchial Epithelial Cell Ferroptosis
Article Snippet: Paraffin Sect. (5 μm) of lung tissue were stained with hematoxylin (Sangon Biotech, Shanghai, China) and eosin (Sangon Biotech, Shanghai, China) for 8 min and 1 min, respectively, and then visualized with BioTek cytting 5 (BioTek, USA) fixed neutral gel (Solarbio, Beijing, China, cat#G8590). .. Paraffin sections of lung tissues were repaired with sodium citrate antigen repair solution (Sangon Biotech, Shanghai, China), followed by inactivation of endogenous catalase with hydrogen peroxide solution and blocking with droplets of goat serum (Sangon Biotech, Shanghai, China). .. The primary antibodies were stored at 4 °C during incubation and included ALOX15 (ABclonal, cat#A6864), a-SMA (ABclonal, cat#A2235), GPX4 (ABclonal, cat#A11243), SLC7A11 (ABclonal, cat#A25302), ACSL4 (ABclonal, cat#A6826), IL-1β (CST, #12242), IL6 (CST, #944070), TNF-α(ABclonal, cat#A21265), and MDA (abcam, #ab27642).

Article Title: Cellular transcriptomics reveals evolutionary adaptation and rumination of vertebrate stomachs
Article Snippet: .. The sections in 1× citrate-based antigen retrieval solution (Sangon Biotech) were heated in a microwave oven, and stood still for 5 min, before being at 100°C for 5 min. After antigen retrieval, tissue sections were blocked with the Immunostaining Blocking Dilution Buffer (Sangon Biotech) for 1 h at room temperature. .. Subsequently, samples were incubated with primary antibody (1:500, Abcam, ab93279) overnight at 4°C.

Centrifugation:

Article Title: Divergent Virulence Traits in Klebsiella Pneumoniae Strains of Capsular Serotype K2 and ST25: Insights from Whole-Genome Sequencing
Article Snippet: .. The bacterial cells were then mixed with 200 μL of 1% Zwittergent (Sangon Biotech) in 100 mM citric acid buffer (Sangon Biotech) at 55°C for 30 min. After centrifugation at 10000 g for 10 min, 0.4 mL of the cell-free supernatant was combined with 1.6 mL of absolute ethanol and incubated for 30 min at 4°C. .. The samples were then centrifuged, rehydrated with 125 μL of water overnight, and mixed with 0.5 mL of 12.5 mM sodium tetraborate (Sigma) in concentrated sulfuric acid (Sinopharm).

Immunostaining:

Article Title: Cellular transcriptomics reveals evolutionary adaptation and rumination of vertebrate stomachs
Article Snippet: .. The sections in 1× citrate-based antigen retrieval solution (Sangon Biotech) were heated in a microwave oven, and stood still for 5 min, before being at 100°C for 5 min. After antigen retrieval, tissue sections were blocked with the Immunostaining Blocking Dilution Buffer (Sangon Biotech) for 1 h at room temperature. .. Subsequently, samples were incubated with primary antibody (1:500, Abcam, ab93279) overnight at 4°C.



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