embryonic fibroblast mef cell line (ATCC)
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embryonic fibroblast mef cell line
Embryonic Fibroblast Mef Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chop/CHOP-KO-DR/pm27977675-59-3-42
Average 93 stars, based on 25 article reviews
Embryonic Fibroblast Mef Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chop/CHOP-KO-DR/pm27977675-59-3-42
Average 93 stars, based on 25 article reviews
embryonic fibroblast mef cell line - by Bioz Stars,
2026-11
93/100 stars
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Control:Article Title: Vitamin K 2 induces non-apoptotic cell death along with autophagosome formation in breast cancer cell lines. Article Snippet: Background Vitamin K2 (VK2) has been reported to induce apoptosis in many types of cancer cells including leukemia.. However, there are no precise reports regarding the breast cancer cells.. From the stand point of clinical implications of VK2 including chemoprevention, we investigated the effects of VK2 on breast cancer cell lines. Article Title: Macrolide Antibiotics Exhibit Cytotoxic Effect under Amino Acid-Depleted Culture Condition by Blocking Autophagy Flux in Head and Neck Squamous Cell Carcinoma Cell Lines. Article Snippet: Cell lines and culture conditions The human oral squamous cell carcinoma cell line CAL 27 and the human pharyngeal carcinoma cell line Detroit 562 obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) were cultured in DMEM plus 10% fetal bovine serum (FBS) (Biowest, Nuaillé, France), and 1% penicillin/streptomycin solution (Wako Pure Chemical Industries). .. A CHOP-/- murine embryonic fibroblast (MEF) cell line (CHOP-KO-DR) established from a 13.5-day-old CHOP-/- mouse embryo by SV-40 immortalization and a CHOP+/+ MEF cell line (DR-wild-type) established by SV-40 immortalization as a Article Title: A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit a-ZDHHC5 Sigma-Aldrich Cat# HPA014670; RRID:AB_2257442 Rabbit a-ZDHHC9 Novus Biologicals Cat# NBP1-84499; RRID:AB_11010738 Mouse a-GOLGA7 Novus Biologicals Cat# H00051125-M01; RRID:AB_2279239 Goat a-FLAG Abcam Cat# ab1257; RRID:AB_299216 Mouse a-HA Biolegend Cat# 901502; RRID:AB_2565007 Rabbit a-HA Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Mouse a-GM130 BD Biosciences Cat# 610822; RRID:AB_398141 Rabbit a-TGN46 Novus Biologicals Cat# NBP1-49643; RRID:AB_10011762 Mouse a-tubulin Millipore Sigma Cat# MS-581; RRID:AB_144075 Goat a-actin Santa Cruz Biotechnology Cat# sc-1616; RRID:AB_630836 Mouse a-actin Santa Cruz Biotechnology Cat# sc-47778; RRID:AB_2714189 Rabbit a-EGFR Cell Signaling Technology Cat# 6627; RRID:AB_10692501 Mouse a-CD71 BioXCell Cat# BE0023; RRID:AB_1107669 Goat polyclonal anti-rabbit Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Life Technologies Cat# A11036; RRID:AB_143011 Goat polyclonal anti-mouse Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A11029; RRID:AB_138404 Donkey polyclonal anti-goat Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Life Technologies Cat# A11057; RRID:AB_142581 Donkey polyclonal anti-rabbit Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A21206; RRID:AB_2535792 Bacterial and Virus Strains IncuCyte NucLight Red Lentivirus Reagent (EF-1a, Puro) Essen BioScience Cat# 4625 Chemicals, Peptides, and Recombinant Proteins CIL56 Acme Bioscience N/A Erastin2 (Compound 35MEW28 in Dixon et al., 2014) Dixon et al., 2014 N/A SYTOX Green Life Technologies Cat# S7020 Dimethyl sulfoxide Sigma-Aldrich Cat# 276855; CAS: 67-68-5 5-(Tetradecyloxy)-2 furoic acid Sigma-Aldrich Cat# T6575 Thapsigargin Sigma-Aldrich Cat# T9033; CAS: 67526-95-8 Staurosporine Sigma-Aldrich Cat# S6942 Cycloheximide Sigma-Aldrich Cat# C7698 2-deoxy-D-glucose Sigma-Aldrich Cat# D8375 Ferrostatin-1 Sigma-Aldrich Cat# SML0583; CAS: 347174-05-4 FCCP Cayman Chemicals Cat# 15218 Necrostatin-1s BioVision Cat# 2263 Bortezomib Fisher Scientific Cat# NC0587961; CAS: 179324-69-7 Q-VD-OPh Fisher Scientific Cat# OPH00101M (Continued on next page) e1 Cell Chemical Biology 26, 1–9.e1–e9, December 19, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Triacsin C Cayman Chemicals Cat# 10007448 9-oxo-9H-fluorene-2,7-disulfonyl dichloride (Compound 2) Sigma Aldrich Cat# CDS013683 1509-0124 (Compound 3) ChemDiv 1509-0124 5267-1376 (Compound 4, CIL56i) ChemDiv 5267-1376 4345-0010 (Compound 5) ChemDiv 4345-0010 1869-0039 (Compound 6) ChemDiv 1869-0039 1509-0058 (Compound 7) ChemDiv 1509-0058 1869-0197 (Compound 8) ChemDiv 1869-0197 Critical Commercial Assays Pierce Microplate BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23252 Deposited Data RNA sequencing data for 293T Control and ZDHHC5KO cells treated ± CIL56 (2.5 mM, 1 h) This paper https://data.mendeley.com/datasets/ m6fp39vyd9/1 Experimental Models: Cell Lines Human: HT-1080 ATCC CCL-121 Mouse: DR-Wildtype ATCC CRL-2977 Mouse: Article Title: A Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Article Snippet: A Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Pin-Joe Ko1, Claire Woodrow1, Michael Dubreuil2, Brent R. Martin3, Rachid Skouta4, Michael C. Bassik2, Scott J. Dixon1* 1Department of Biology, Stanford University, Stanford CA 94305, USA 2Department of Genetics, Stanford University School of Medicine, Stanford CA, 94305, USA 3Department of Chemistry, University of Michigan, Ann Arbor MI, 48109, USA 4Department of Biology, University of Massachusetts Amherst, Amherst MA, 01003, USA other:Article Title: Multi-stage synthesis method with synthesis gas Article Snippet: Journal of Biotechnology, Volume 14, Issue 2, pages 187-194, in Li Production of acetic acid from synthesis gas with mixed acetogenic microorganisms, ISSN 0493644938, in Schmidt et al. Production of acetic acid from hydrogen and carbon dioxide by Cell Culture:Article Title: Macrolide Antibiotics Exhibit Cytotoxic Effect under Amino Acid-Depleted Culture Condition by Blocking Autophagy Flux in Head and Neck Squamous Cell Carcinoma Cell Lines. Article Snippet: Cell lines and culture conditions The human oral squamous cell carcinoma cell line CAL 27 and the human pharyngeal carcinoma cell line Detroit 562 obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) were cultured in DMEM plus 10% fetal bovine serum (FBS) (Biowest, Nuaillé, France), and 1% penicillin/streptomycin solution (Wako Pure Chemical Industries). .. A CHOP-/- murine embryonic fibroblast (MEF) cell line (CHOP-KO-DR) established from a 13.5-day-old CHOP-/- mouse embryo by SV-40 immortalization and a CHOP+/+ MEF cell line (DR-wild-type) established by SV-40 immortalization as a Bicinchoninic Acid Protein Assay:Article Title: A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit a-ZDHHC5 Sigma-Aldrich Cat# HPA014670; RRID:AB_2257442 Rabbit a-ZDHHC9 Novus Biologicals Cat# NBP1-84499; RRID:AB_11010738 Mouse a-GOLGA7 Novus Biologicals Cat# H00051125-M01; RRID:AB_2279239 Goat a-FLAG Abcam Cat# ab1257; RRID:AB_299216 Mouse a-HA Biolegend Cat# 901502; RRID:AB_2565007 Rabbit a-HA Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Mouse a-GM130 BD Biosciences Cat# 610822; RRID:AB_398141 Rabbit a-TGN46 Novus Biologicals Cat# NBP1-49643; RRID:AB_10011762 Mouse a-tubulin Millipore Sigma Cat# MS-581; RRID:AB_144075 Goat a-actin Santa Cruz Biotechnology Cat# sc-1616; RRID:AB_630836 Mouse a-actin Santa Cruz Biotechnology Cat# sc-47778; RRID:AB_2714189 Rabbit a-EGFR Cell Signaling Technology Cat# 6627; RRID:AB_10692501 Mouse a-CD71 BioXCell Cat# BE0023; RRID:AB_1107669 Goat polyclonal anti-rabbit Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Life Technologies Cat# A11036; RRID:AB_143011 Goat polyclonal anti-mouse Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A11029; RRID:AB_138404 Donkey polyclonal anti-goat Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Life Technologies Cat# A11057; RRID:AB_142581 Donkey polyclonal anti-rabbit Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A21206; RRID:AB_2535792 Bacterial and Virus Strains IncuCyte NucLight Red Lentivirus Reagent (EF-1a, Puro) Essen BioScience Cat# 4625 Chemicals, Peptides, and Recombinant Proteins CIL56 Acme Bioscience N/A Erastin2 (Compound 35MEW28 in Dixon et al., 2014) Dixon et al., 2014 N/A SYTOX Green Life Technologies Cat# S7020 Dimethyl sulfoxide Sigma-Aldrich Cat# 276855; CAS: 67-68-5 5-(Tetradecyloxy)-2 furoic acid Sigma-Aldrich Cat# T6575 Thapsigargin Sigma-Aldrich Cat# T9033; CAS: 67526-95-8 Staurosporine Sigma-Aldrich Cat# S6942 Cycloheximide Sigma-Aldrich Cat# C7698 2-deoxy-D-glucose Sigma-Aldrich Cat# D8375 Ferrostatin-1 Sigma-Aldrich Cat# SML0583; CAS: 347174-05-4 FCCP Cayman Chemicals Cat# 15218 Necrostatin-1s BioVision Cat# 2263 Bortezomib Fisher Scientific Cat# NC0587961; CAS: 179324-69-7 Q-VD-OPh Fisher Scientific Cat# OPH00101M (Continued on next page) e1 Cell Chemical Biology 26, 1–9.e1–e9, December 19, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Triacsin C Cayman Chemicals Cat# 10007448 9-oxo-9H-fluorene-2,7-disulfonyl dichloride (Compound 2) Sigma Aldrich Cat# CDS013683 1509-0124 (Compound 3) ChemDiv 1509-0124 5267-1376 (Compound 4, CIL56i) ChemDiv 5267-1376 4345-0010 (Compound 5) ChemDiv 4345-0010 1869-0039 (Compound 6) ChemDiv 1869-0039 1509-0058 (Compound 7) ChemDiv 1509-0058 1869-0197 (Compound 8) ChemDiv 1869-0197 Critical Commercial Assays Pierce Microplate BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23252 Deposited Data RNA sequencing data for 293T Control and ZDHHC5KO cells treated ± CIL56 (2.5 mM, 1 h) This paper https://data.mendeley.com/datasets/ m6fp39vyd9/1 Experimental Models: Cell Lines Human: HT-1080 ATCC CCL-121 Mouse: DR-Wildtype ATCC CRL-2977 Mouse: Article Title: A Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Article Snippet: A Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Pin-Joe Ko1, Claire Woodrow1, Michael Dubreuil2, Brent R. Martin3, Rachid Skouta4, Michael C. Bassik2, Scott J. Dixon1* 1Department of Biology, Stanford University, Stanford CA 94305, USA 2Department of Genetics, Stanford University School of Medicine, Stanford CA, 94305, USA 3Department of Chemistry, University of Michigan, Ann Arbor MI, 48109, USA 4Department of Biology, University of Massachusetts Amherst, Amherst MA, 01003, USA RNA Sequencing:Article Title: A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit a-ZDHHC5 Sigma-Aldrich Cat# HPA014670; RRID:AB_2257442 Rabbit a-ZDHHC9 Novus Biologicals Cat# NBP1-84499; RRID:AB_11010738 Mouse a-GOLGA7 Novus Biologicals Cat# H00051125-M01; RRID:AB_2279239 Goat a-FLAG Abcam Cat# ab1257; RRID:AB_299216 Mouse a-HA Biolegend Cat# 901502; RRID:AB_2565007 Rabbit a-HA Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Mouse a-GM130 BD Biosciences Cat# 610822; RRID:AB_398141 Rabbit a-TGN46 Novus Biologicals Cat# NBP1-49643; RRID:AB_10011762 Mouse a-tubulin Millipore Sigma Cat# MS-581; RRID:AB_144075 Goat a-actin Santa Cruz Biotechnology Cat# sc-1616; RRID:AB_630836 Mouse a-actin Santa Cruz Biotechnology Cat# sc-47778; RRID:AB_2714189 Rabbit a-EGFR Cell Signaling Technology Cat# 6627; RRID:AB_10692501 Mouse a-CD71 BioXCell Cat# BE0023; RRID:AB_1107669 Goat polyclonal anti-rabbit Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Life Technologies Cat# A11036; RRID:AB_143011 Goat polyclonal anti-mouse Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A11029; RRID:AB_138404 Donkey polyclonal anti-goat Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Life Technologies Cat# A11057; RRID:AB_142581 Donkey polyclonal anti-rabbit Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A21206; RRID:AB_2535792 Bacterial and Virus Strains IncuCyte NucLight Red Lentivirus Reagent (EF-1a, Puro) Essen BioScience Cat# 4625 Chemicals, Peptides, and Recombinant Proteins CIL56 Acme Bioscience N/A Erastin2 (Compound 35MEW28 in Dixon et al., 2014) Dixon et al., 2014 N/A SYTOX Green Life Technologies Cat# S7020 Dimethyl sulfoxide Sigma-Aldrich Cat# 276855; CAS: 67-68-5 5-(Tetradecyloxy)-2 furoic acid Sigma-Aldrich Cat# T6575 Thapsigargin Sigma-Aldrich Cat# T9033; CAS: 67526-95-8 Staurosporine Sigma-Aldrich Cat# S6942 Cycloheximide Sigma-Aldrich Cat# C7698 2-deoxy-D-glucose Sigma-Aldrich Cat# D8375 Ferrostatin-1 Sigma-Aldrich Cat# SML0583; CAS: 347174-05-4 FCCP Cayman Chemicals Cat# 15218 Necrostatin-1s BioVision Cat# 2263 Bortezomib Fisher Scientific Cat# NC0587961; CAS: 179324-69-7 Q-VD-OPh Fisher Scientific Cat# OPH00101M (Continued on next page) e1 Cell Chemical Biology 26, 1–9.e1–e9, December 19, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER Triacsin C Cayman Chemicals Cat# 10007448 9-oxo-9H-fluorene-2,7-disulfonyl dichloride (Compound 2) Sigma Aldrich Cat# CDS013683 1509-0124 (Compound 3) ChemDiv 1509-0124 5267-1376 (Compound 4, CIL56i) ChemDiv 5267-1376 4345-0010 (Compound 5) ChemDiv 4345-0010 1869-0039 (Compound 6) ChemDiv 1869-0039 1509-0058 (Compound 7) ChemDiv 1509-0058 1869-0197 (Compound 8) ChemDiv 1869-0197 Critical Commercial Assays Pierce Microplate BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23252 Deposited Data RNA sequencing data for 293T Control and ZDHHC5KO cells treated ± CIL56 (2.5 mM, 1 h) This paper https://data.mendeley.com/datasets/ m6fp39vyd9/1 Experimental Models: Cell Lines Human: HT-1080 ATCC CCL-121 Mouse: DR-Wildtype ATCC CRL-2977 Mouse: |