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ultrascript 2 0 cdna synthesis kit  (PCR Biosystems Ltd)


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    Structured Review

    PCR Biosystems Ltd ultrascript 2 0 cdna synthesis kit
    Ultrascript 2 0 Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+reverse+transcriptase+kit/UltraScript+2%2E0+Reverse+Transcriptase/pm42854383-58-0-14
    Average 94 stars, based on 89 article reviews
    ultrascript 2 0 cdna synthesis kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    SYBR Green Assay:

    Article Title: Integrated Phenomic and Transcriptomic Network Analysis Reveals Key Regulators of Drought Tolerance in OsC2H2-Edited Rice
    Article Snippet: Libraries were sequenced on an Illumina NovaSeq X platform (Macrogen, Seoul, Korea) as paired-end 150-bp reads. .. First-strand cDNA synthesis and subsequent quantitative PCR (QRT-PCR) were performed using an UltraScript 2.0 cDNA Synthesis Kit (PCRBIOSYSTEM, Wayne, PA, USA) and TOPreal SYBR Green qPCR PreMIX (Enzynomics, Daejeon, Republic of Korea) following the manufacturers’ instructions. .. Amplification was carried out on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 35 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s after initial denaturation at 95°C for 10 min.

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).

    Reverse Transcription:

    Article Title: Brief, repeated isoflurane anaesthesia elicits sex-dependent behavioural, cerebrovascular, and microglial alterations in mice
    Article Snippet: .. Total RNA was prepared using TRIzol reagent (Life Technologies Ltd.) and then reverse-transcribed with Ultrascript 2.0 reverse transcriptase (PCRBiosystems Ltd., London, UK) according to the manufacturer’s protocols. .. Resultant cDNA was then analysed by real-time PCR in duplicate, using the Quantitect primer system (primer sets: Cldn5 QT00254905, Dlg4 QT00121695, Il1b QT01048355, Il10 QT00106169, Ocln QT00111055, Pecam1 QT01052044, Syp QT01042314, Tjp1 QT00493899 and Tnfa QT00029162; all Qiagen Ltd.) and SyGreen (Blue) Lo-ROX master mix (PCRBiosystems Ltd., UK), according to conditions consisting of 95°C for 2 minutes, then 40 cycles of 95°C for 5 seconds, 55°C for 25 seconds, and 72°C for 20 seconds.

    Polymerase Chain Reaction:

    Article Title: Ent-7β-hydroxy-15-oxokaur-16-en-18-yl acetate suppresses RANKL-induced and LPS-enhanced osteoclastogenesis through NF-κB/MAPK signaling with involvement of the ACE-AT1R axis.
    Article Snippet: TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). .. UltraScript 2.0 cDNA synthesis kit and SYBR Green PCR Master Mix were obtained from PCR Biosystems Ltd. (London, UK). .. Amersham Protran Premium Western blotting membranes were purchased from Cytiva (Marlborough, MA, USA).



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