reverse transcriptase kit (TaKaRa)
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TaKaRa
reverse transcriptase kit
Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+reverse+transcriptase+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/us12600957-257-0-12
Average 99 stars, based on 2331 article reviews
Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+reverse+transcriptase+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/us12600957-257-0-12
Average 99 stars, based on 2331 article reviews
reverse transcriptase kit - by Bioz Stars,
2026-10
99/100 stars
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Reverse Transcription:Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer. Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the Article Title: IL-15 and IL-21 synergy improves anti-tumor efficacy of iPSC-derived cytotoxic T cells in solid tumors. Article Snippet: .. We reverse transcribed mRNA using a Article Title: Exon skipping by peptide nucleic acid derivatives Article Snippet: Then the culture medium was replaced with fresh medium, and treated with “PD-ASO 3” at 0 (negative control), 10, 100 or 1,000 aM for 24 hours. (4 culture dishes per ASO concentration) [RNA Extraction and cDNA Synthesis] Total RNA was extracted using RNeasy mini prep kit (Qiagen, USA) according to the manufacturer's protocol. .. 500 ng of RNA template was subjected to a 25 μL reverse transcription reaction using cDNA Synthesis:Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer. Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the Article Title: Method of alleviating obsessive compulsive disorder symptoms by use of a multi-strain probiotic formulation Article Snippet: Briefly, NucleoSpin® RNA isolation kit (#740955.50, DSS Takara Bio India Private Ltd) was used to extract RNA from the rat brain's amygdala. .. Article Title: Discovery of acyclic purine nucleosides as promising antiviral agents against dengue virus. Article Snippet: Dengue virus (DENV) remains a significant global health challenge as one of the most prevalent mosquito-borne viral infections, with no specific antiviral therapy currently approved.. Acyclic purine nucleosides have demonstrated as broad-spectrum antiviral activity by interfering with viral genome replication.. Therefore, in this study, we designed a new series of D-glucose-derived acyclic purine nucleoside analogues (5a-f, 6a-c, and 8a-b), and evaluated their inhibitory activity against DENV serotype 2 (DENV2) using an in vitro Huh-7 cell-based assay. Article Title: A novel hemizygous missense variant in the BEND2 gene is associated with nonobstructive azoospermia. Article Snippet: After the cells were washed with phosphatebuffered saline (PBS), the sections were incubated with Alexa Fluor 488-conjugated anti-rabbit IgG (A-11008; 1:500; Invitrogen, Carlsbad, CA, USA) and peanut agglutinin (PNA; CL-1073-1; 1:500; Vector Labs, Burlingame, CA, USA) for 2 h at room temperature, after which the nuclei were counterstained with Hoechst 33342 (H3570; 1:5000; Invitrogen). .. Imaging was performed on a Zeiss LSM 980 confocal system (ZEISS, Oberkochen, Germany). mRNA expression analysis Total RNA was isolated from the peripheral blood of the NOA proband and OA control, after which first-strand complementary DNA (cDNA) synthesis was performed using a Takara Article Title: High Fat Diet‐Induced Obesity Alters Cutaneous Immune Cell Function, and These Changes Persist After Weight Loss Article Snippet: Total RNA was extracted from the tissues using ISOGEN (Nippon Gene CO. Ltd., Tokyo, Japan). .. Complementary deoxyribonucleic acid (cDNA) was synthesized using the Article Title: IL-15 and IL-21 synergy improves anti-tumor efficacy of iPSC-derived cytotoxic T cells in solid tumors. Article Snippet: .. We reverse transcribed mRNA using a Article Title: Dual-compartment engagement of STAR-family proteins SAM68 and QKI by LINC00941 sustains oncogenic fitness in RAS-driven lung cancer Article Snippet: RNA quantity and integrity were assessed by NanoDrop spectrophotometry and agarose gel electrophoresis, respectively. .. Complementary DNA (cDNA) was synthesised from 500 ng–1 μ g total RNA using the Article Title: Exon skipping by peptide nucleic acid derivatives Article Snippet: Then the culture medium was replaced with fresh medium, and treated with “PD-ASO 3” at 0 (negative control), 10, 100 or 1,000 aM for 24 hours. (4 culture dishes per ASO concentration) [RNA Extraction and cDNA Synthesis] Total RNA was extracted using RNeasy mini prep kit (Qiagen, USA) according to the manufacturer's protocol. .. 500 ng of RNA template was subjected to a 25 μL reverse transcription reaction using Quantitative RT-PCR:Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer. Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the Real-time Polymerase Chain Reaction:Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer. Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the Isolation:Article Title: Discovery of acyclic purine nucleosides as promising antiviral agents against dengue virus. Article Snippet: Dengue virus (DENV) remains a significant global health challenge as one of the most prevalent mosquito-borne viral infections, with no specific antiviral therapy currently approved.. Acyclic purine nucleosides have demonstrated as broad-spectrum antiviral activity by interfering with viral genome replication.. Therefore, in this study, we designed a new series of D-glucose-derived acyclic purine nucleoside analogues (5a-f, 6a-c, and 8a-b), and evaluated their inhibitory activity against DENV serotype 2 (DENV2) using an in vitro Huh-7 cell-based assay. Article Title: A novel hemizygous missense variant in the BEND2 gene is associated with nonobstructive azoospermia. Article Snippet: After the cells were washed with phosphatebuffered saline (PBS), the sections were incubated with Alexa Fluor 488-conjugated anti-rabbit IgG (A-11008; 1:500; Invitrogen, Carlsbad, CA, USA) and peanut agglutinin (PNA; CL-1073-1; 1:500; Vector Labs, Burlingame, CA, USA) for 2 h at room temperature, after which the nuclei were counterstained with Hoechst 33342 (H3570; 1:5000; Invitrogen). .. Imaging was performed on a Zeiss LSM 980 confocal system (ZEISS, Oberkochen, Germany). mRNA expression analysis Total RNA was isolated from the peripheral blood of the NOA proband and OA control, after which first-strand complementary DNA (cDNA) synthesis was performed using a Takara Imaging:Article Title: A novel hemizygous missense variant in the BEND2 gene is associated with nonobstructive azoospermia. Article Snippet: After the cells were washed with phosphatebuffered saline (PBS), the sections were incubated with Alexa Fluor 488-conjugated anti-rabbit IgG (A-11008; 1:500; Invitrogen, Carlsbad, CA, USA) and peanut agglutinin (PNA; CL-1073-1; 1:500; Vector Labs, Burlingame, CA, USA) for 2 h at room temperature, after which the nuclei were counterstained with Hoechst 33342 (H3570; 1:5000; Invitrogen). .. Imaging was performed on a Zeiss LSM 980 confocal system (ZEISS, Oberkochen, Germany). mRNA expression analysis Total RNA was isolated from the peripheral blood of the NOA proband and OA control, after which first-strand complementary DNA (cDNA) synthesis was performed using a Takara Expressing:Article Title: A novel hemizygous missense variant in the BEND2 gene is associated with nonobstructive azoospermia. Article Snippet: After the cells were washed with phosphatebuffered saline (PBS), the sections were incubated with Alexa Fluor 488-conjugated anti-rabbit IgG (A-11008; 1:500; Invitrogen, Carlsbad, CA, USA) and peanut agglutinin (PNA; CL-1073-1; 1:500; Vector Labs, Burlingame, CA, USA) for 2 h at room temperature, after which the nuclei were counterstained with Hoechst 33342 (H3570; 1:5000; Invitrogen). .. Imaging was performed on a Zeiss LSM 980 confocal system (ZEISS, Oberkochen, Germany). mRNA expression analysis Total RNA was isolated from the peripheral blood of the NOA proband and OA control, after which first-strand complementary DNA (cDNA) synthesis was performed using a Takara Control:Article Title: A novel hemizygous missense variant in the BEND2 gene is associated with nonobstructive azoospermia. Article Snippet: After the cells were washed with phosphatebuffered saline (PBS), the sections were incubated with Alexa Fluor 488-conjugated anti-rabbit IgG (A-11008; 1:500; Invitrogen, Carlsbad, CA, USA) and peanut agglutinin (PNA; CL-1073-1; 1:500; Vector Labs, Burlingame, CA, USA) for 2 h at room temperature, after which the nuclei were counterstained with Hoechst 33342 (H3570; 1:5000; Invitrogen). .. Imaging was performed on a Zeiss LSM 980 confocal system (ZEISS, Oberkochen, Germany). mRNA expression analysis Total RNA was isolated from the peripheral blood of the NOA proband and OA control, after which first-strand complementary DNA (cDNA) synthesis was performed using a Takara Synthesized:Article Title: High Fat Diet‐Induced Obesity Alters Cutaneous Immune Cell Function, and These Changes Persist After Weight Loss Article Snippet: Total RNA was extracted from the tissues using ISOGEN (Nippon Gene CO. Ltd., Tokyo, Japan). .. Complementary deoxyribonucleic acid (cDNA) was synthesized using the |