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4×44k oligonucleotide two-colour cdna maize microarray slides  (Agilent technologies)


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    Agilent technologies 4×44k oligonucleotide two-colour cdna maize microarray slides
    4×44k Oligonucleotide Two Colour Cdna Maize Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+slide/pmc04085969-139-9-8
    Average 90 stars, based on 1 article reviews
    4×44k oligonucleotide two-colour cdna maize microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: SWISS MADE: Standardized WithIn Class Sum of Squares to Evaluate Methodologies and Dataset Elements
    Article Snippet: , IV b , HNSCC , Agilent 4×44K oligonucleotide arrays , Self hybridization , 16 , Tumor/Normal , .

    Article Title: Lung Adenocarcinoma of Never Smokers and Smokers Harbor Differential Regions of Genetic Alteration and Exhibit Different Levels of Genomic Instability
    Article Snippet: Normalized, lung adenocarcinoma Agilent 4×44 k array comparative genomic hybridization (aCGH) data generated by the Memorial Sloan Kettering Cancer Centre (MSKCC) was obtained from http://cbio.mskcc.org/Public/lung_array_data/ .

    Article Title: SWISS MADE: Standardized WithIn Class Sum of Squares to Evaluate Methodologies and Dataset Elements
    Article Snippet: , III a , HNSCC , Agilent 4×44K oligonucleotide arrays , Reference design , 16 , Tumor/Normal , .

    Article Title: Acidosis Activation of the Proton-Sensing GPR4 Receptor Stimulates Vascular Endothelial Cell Inflammatory Responses Revealed by Transcriptome Analysis
    Article Snippet: Microarray was performed on Agilent 4×44K human whole genome microarray chips at the Genomics and Bioinformatics Core of the University of North Carolina, Chapel Hill.

    Article Title: DC Priming by M. vaccae Inhibits Th2 Responses in Contrast to Specific TLR2 Priming and Is Associated with Selective Activation of the CREB Pathway
    Article Snippet: DC culture lysates collected in RLT buffer (Qiagen) were used to purify total RNA and generate Cy3 or Cy5 labelled cRNA for hybridization with Agilent 4×44K whole human genome cDNA microarrays and data acquisition as previously described .

    Labeling:

    Article Title: Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
    Article Snippet: For each RNA sample, a single microarray was hybridized with 750 ng Cy3 labeled cDNA using Agilent's standard conditions for single-color microarrays at the Whitehead Center for Microarray Technology. .. The Agilent Low-Input QuickAmp Labeling Kit was used for labeling, the samples were hybridized to a the custom Agilent 4 × 44K feature zebrafish microarray using the Agilent In situ Hybridization Kit Plus, and labeled cDNA was combined with the Agilent 10× Control Targets (to identify microarray corners). .. Post-hybridization, microarray slides were washed as per the Agilent In situ Hybridization Kit Plus.

    Microarray:

    Article Title: Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
    Article Snippet: For each RNA sample, a single microarray was hybridized with 750 ng Cy3 labeled cDNA using Agilent's standard conditions for single-color microarrays at the Whitehead Center for Microarray Technology. .. The Agilent Low-Input QuickAmp Labeling Kit was used for labeling, the samples were hybridized to a the custom Agilent 4 × 44K feature zebrafish microarray using the Agilent In situ Hybridization Kit Plus, and labeled cDNA was combined with the Agilent 10× Control Targets (to identify microarray corners). .. Post-hybridization, microarray slides were washed as per the Agilent In situ Hybridization Kit Plus.

    In Situ Hybridization:

    Article Title: Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
    Article Snippet: For each RNA sample, a single microarray was hybridized with 750 ng Cy3 labeled cDNA using Agilent's standard conditions for single-color microarrays at the Whitehead Center for Microarray Technology. .. The Agilent Low-Input QuickAmp Labeling Kit was used for labeling, the samples were hybridized to a the custom Agilent 4 × 44K feature zebrafish microarray using the Agilent In situ Hybridization Kit Plus, and labeled cDNA was combined with the Agilent 10× Control Targets (to identify microarray corners). .. Post-hybridization, microarray slides were washed as per the Agilent In situ Hybridization Kit Plus.

    Control:

    Article Title: Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
    Article Snippet: For each RNA sample, a single microarray was hybridized with 750 ng Cy3 labeled cDNA using Agilent's standard conditions for single-color microarrays at the Whitehead Center for Microarray Technology. .. The Agilent Low-Input QuickAmp Labeling Kit was used for labeling, the samples were hybridized to a the custom Agilent 4 × 44K feature zebrafish microarray using the Agilent In situ Hybridization Kit Plus, and labeled cDNA was combined with the Agilent 10× Control Targets (to identify microarray corners). .. Post-hybridization, microarray slides were washed as per the Agilent In situ Hybridization Kit Plus.



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    Image Search Results


    Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Journal: Scientific Reports

    Article Title: Extracellular matrix stiffness dictates Wnt expression through integrin pathway

    doi: 10.1038/srep20395

    Figure Lengend Snippet: Results from primary chondrocytes 48 hr after seeding on stiff (100 kPa) or soft (0.5–1 kPa) ECM. ( a ) Microarray profiling of Wnt/β-catenin pathway transcripts. Results are normalized by median scaling using Rosetta Resolver System software. ( b ) Wnt1 and Wnt3a levels were analyzed by western blotting. ( c ) Total and phosphorylated ERK1/2 levels were analyzed by western blotting. ( d ) Axin2, CD44, and ( e ) phosphorylated GSK3β levels were analyzed by western blotting. ( f ) Total and phosphorylated β−catenin levels were analyzed by western blotting. ( g ) β−catenin levels in nucleus and cytoplasm were analyzed by western blotting. ( h ) Total and ( i ) activated β-catenin levels and distribution in chondrocytes 2 hr after seeding on stiff or soft ECM were analyzed by in situ fluorescence staining. ( j ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the Matrigel-coated PAAM were analyzed by western blotting. ( k ) β-catenin and wnt1 levels in chondrocytes 48 hr after seeding on the ColII-coated PAAM were analyzed by western blotting. Western results were from 3 independent experiments for each individual protein, with blots exemplifying one experiment and the bar graphs showing the combined results of 3 experiments on stiff matrix expressed as percentages (mean ± SEM) of the corresponding results on the soft matrix. GAPDH was used to normalize for equal loading. *P < 0.05, **P < 0.01. n.s. stands for not statistically significant.

    Article Snippet: Microarray analyses were performed using commercial Mouse cDNA Microarray slides (Phalanx Biotech Group; Hsinchu, Taiwan) according to the manufacturer’s instructions.

    Techniques: Microarray, Software, Western Blot, In Situ, Fluorescence, Staining