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cdna microarray experiment  (Welgene Biotech)

 
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    Welgene Biotech cdna microarray experiment
    Cdna Microarray Experiment, supplied by Welgene Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+experiments/microarray+analysis/pm18309246-40-1-7
    Average 90 stars, based on 1 article reviews
    cdna microarray experiment - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis
    Article Snippet: .. The microarray analysis was performed by Welgene Biotech (Taipei, Taiwan) as in our previous study [ ]. .. In brief, total RNA was extracted by TRIzol reagent (Invitrogen, Carlsbad, CA, USA), then quantified at OD 260nm by an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and quantified using a Bioanalyzer 2100 (Agilent Technology, USA) with an RNA 6000 lab chip kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
    Article Snippet: .. Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

    Article Title: Switched phenotypes of macrophages during the different stages of Schistosoma japonicum infection influenced the subsequent trends of immune responses.
    Article Snippet: .. The microarray experiment was also commissioned to Welgene Biotech, Co., Ltd. to make the experimental analysis of gene arrays. .. This experiment adopted Agilent Technologies, Inc. (CA) and Agilent custom mouse immune arrays.

    Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
    Article Snippet: .. Gene expression microarray and gene set enrichment analysis Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

    Article Title: Ultrarapid Inflammation of the Olfactory Bulb After Spinal Cord Injury: Protective Effects of the Granulocyte Colony-Stimulating Factor on Early Neurodegeneration in the Brain
    Article Snippet: .. Microarray analysis was performed using the Agilent mouse gene expression microarray kit (Welgene Biotech, Taipei, Taiwan), following the manufacturer’s instructions. .. Briefly, 0.2 μg of total RNA extracted from the whole-brain lysates was labeled with Cy3 (CyDye, Agilent Technologies, Santa Clara, CA, United States) using the Low Input Quick-Amp labeling kit (Agilent Technologies, Santa Clara, CA, United States) during the in vitro transcription process.

    Article Title: Increased Expression of Long Noncoding RNA LOC100506314 in T cells from Patients with Nonsegmental Vitiligo and Its Contribution to Vitiligo Pathogenesis
    Article Snippet: Then anti-human CD4-coated magnetic beads and anti-human CD8-coated magnetic beads were used to separate the T cells, CD4+ T cells, or CD8+ T cells by IMag cell separation system (BD Bioscience, Franklin Lakes, NJ, USA). .. The gene expression profiles of T cells obtained from three female vitiligo patients aged 44, 46, and 48 years and three female controls aged 45, 47, and 48 years were investigated using the microarray analysis (Welgene Biotech, Taipei, Taiwan) as previously described [ ]. .. We extracted total RNA by the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).

    Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2
    Article Snippet: .. TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service. .. The labeling of 100 ng of total RNA was performed using the Low Input Quick Amp Labeling Kit (Agilent Technologies).

    Expressing:

    Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
    Article Snippet: .. Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

    Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
    Article Snippet: .. Gene expression microarray and gene set enrichment analysis Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

    Gene Expression:

    Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
    Article Snippet: .. Gene expression microarray and gene set enrichment analysis Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

    Article Title: Ultrarapid Inflammation of the Olfactory Bulb After Spinal Cord Injury: Protective Effects of the Granulocyte Colony-Stimulating Factor on Early Neurodegeneration in the Brain
    Article Snippet: .. Microarray analysis was performed using the Agilent mouse gene expression microarray kit (Welgene Biotech, Taipei, Taiwan), following the manufacturer’s instructions. .. Briefly, 0.2 μg of total RNA extracted from the whole-brain lysates was labeled with Cy3 (CyDye, Agilent Technologies, Santa Clara, CA, United States) using the Low Input Quick-Amp labeling kit (Agilent Technologies, Santa Clara, CA, United States) during the in vitro transcription process.

    Article Title: Increased Expression of Long Noncoding RNA LOC100506314 in T cells from Patients with Nonsegmental Vitiligo and Its Contribution to Vitiligo Pathogenesis
    Article Snippet: Then anti-human CD4-coated magnetic beads and anti-human CD8-coated magnetic beads were used to separate the T cells, CD4+ T cells, or CD8+ T cells by IMag cell separation system (BD Bioscience, Franklin Lakes, NJ, USA). .. The gene expression profiles of T cells obtained from three female vitiligo patients aged 44, 46, and 48 years and three female controls aged 45, 47, and 48 years were investigated using the microarray analysis (Welgene Biotech, Taipei, Taiwan) as previously described [ ]. .. We extracted total RNA by the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).

    Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2
    Article Snippet: .. TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service. .. The labeling of 100 ng of total RNA was performed using the Low Input Quick Amp Labeling Kit (Agilent Technologies).

    other:

    Article Title: The AtERF19 gene regulates meristem activity and flower organ size in plants.
    Article Snippet: Ethylene-responsive factors (ERFs) have diverse functions in the regulation of various plant developmental processes.. Here, we demonstrate the dual role of an Arabidopsis ERF gene, AtERF19, in regulating reproductive meristem activity and flower organ size through the regulation of genes involved in CLAVATA– WUSCHEL (CLV–WUS) and auxin signaling, respectively.. We found that AtERF19 stimulated the formation of flower primordia and controlled the number of flowers produced by activating WUS and was negatively regulated by CLV3.



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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    The RNA samples prepared for Affymetrix <t>microarray</t> experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.
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    Image Search Results


    The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Western Blot, Expressing, Transfection, Electrophoresis

    Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Quantitative RT-PCR, Blocking Assay, Real-time Polymerase Chain Reaction

    Verification of microarray data by quantitative real-time PCR .

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Verification of microarray data by quantitative real-time PCR .

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay