cdna oligonucleotide microarray data analysis (Thermo Fisher)

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Thermo Fisher cdna oligonucleotide microarray data analysis
Cdna Oligonucleotide Microarray Data Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+microarray+data/pmc02771024-127-18-19
Average 90 stars, based on 1 article reviews

cdna oligonucleotide microarray data analysis - by Bioz Stars, 2026-09

90/100 stars

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Labeling:

Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response
Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-κB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-κB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled oligonucleotide (Invitrogen) were incubated at room temperature for 1 h in binding buffer. ..

Article Title: Castanea crenata honey reduces influenza infection by activating the innate immune response.
Article Snippet: EMSA was performed to determine the DNA-binding activity of NF-kB in influenza-infected cells with a DIG-labeled oligonucleotide (NF-kB: 5′-AGT TGA GGG GAC TTT CCC AGG C-3′) using nonradioactive EMSA kit (Roche, Mannheim, Germany). .. The nuclear extracts and labeled oligonucleotide (Invitrogen) were incubated at room temperature for 1 h in binding buffer. ..

Incubation:

Binding Assay:

Plasmid Preparation:

Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents
Article Snippet: The resulting DNA insert was purified using a commercial DNA purification kit (Macherey-Nagel) according to the protocol. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Oligonucleotide (Thermo) for 16 h at 37°C, followed by purification using a commercial DNA purification kit (Macherey-Nagel). .. The solution of linearized plasmid was incubated (50 μl) with DNA Polymerase I Large (Klenow) Fragment (15 U), 10 × buffer 3 (NEB), and 10 mM NTP (Thermo) at 25°C for 15 min, to remove 3′ overhangs and filling in 5′ overhangs to form blunt ends.

Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents.
Article Snippet: The resulting DNA insert was purified using a commercial DNA purification kit (Macherey-Nagel) according to the protocol. .. The circular plasmid pJET1.2_Fluc (of ∼30 adenine nucleotide tail) encoding Firefly Luciferase (2 μg) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Oligonucleotide (Thermo) for 16 h at 37 ◦C, followed by purification using a commercial DNA purification kit (Macherey-Nagel). .. Blunt ends vector formation The solution of linearized plasmid was incubated (50 μl) with DNA Polymerase I Large (Klenow) Fragment (15 U), 10 × buffer 3 (NEB), and 10 mM NTP (Thermo) at 25 ◦C for 15 min, to remove 3 ′ overhangs and filling in 5 ′ overhangs to form blunt ends.

Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents
Article Snippet: The digested DNA was then resolved on 1% agarose gel (1 × TAE) and the band containing the FLuc sequence was excised and purified using a commercial DNA purification kit (Macherey-Nagel) according to the manufacturer protocol affording the pure insert. .. The pJet1.2 plasmid vector (2 μg, Thermo) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Oligonucleotide (Thermo) for 16 h at 37°C, followed by purification using a commercial DNA purification kit (Macherey-Nagel). .. The resulting pJET1.2 vector solution containing 100 ng of DNA was mixed with the insert (FLuc sequence) at a molar ratio of 1:3 (vector:insert).

Luciferase:

Purification:

Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents
Article Snippet: The digested DNA was then resolved on 1% agarose gel (1 × TAE) and the band containing the FLuc sequence was excised and purified using a commercial DNA purification kit (Macherey-Nagel) according to the manufacturer protocol affording the pure insert. .. The pJet1.2 plasmid vector (2 μg, Thermo) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Oligonucleotide (Thermo) for 16 h at 37°C, followed by purification using a commercial DNA purification kit (Macherey-Nagel). .. The resulting pJET1.2 vector solution containing 100 ng of DNA was mixed with the insert (FLuc sequence) at a molar ratio of 1:3 (vector:insert).

DNA Purification:

Article Title: Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents
Article Snippet: The digested DNA was then resolved on 1% agarose gel (1 × TAE) and the band containing the FLuc sequence was excised and purified using a commercial DNA purification kit (Macherey-Nagel) according to the manufacturer protocol affording the pure insert. .. The pJet1.2 plasmid vector (2 μg, Thermo) was linearized (50 μl reaction volume) by digestion with AarI restriction enzyme (6 μl, Thermo) in 10 × AarI buffer (Thermo) and 50 × Oligonucleotide (Thermo) for 16 h at 37°C, followed by purification using a commercial DNA purification kit (Macherey-Nagel). .. The resulting pJET1.2 vector solution containing 100 ng of DNA was mixed with the insert (FLuc sequence) at a molar ratio of 1:3 (vector:insert).

Negative Control:

Article Title: BECN1 promotes the migration of NSCLC cells through regulating the ubiquitination of Vimentin
Article Snippet: .. A nonspecific oligonucleotide from Thermo Fisher Scientific was used as a negative control. ..

Multiplex Assay:

Article Title: Development of cost-effective and fast KIR genotyping by multiplex PCR-SSP.
Article Snippet: .. These samples were then typed using the multiplex PCR-SSP method and a Luminex-based reverse sequence-specific oligonucleotide typing systems from One Lambda, Inc. (Canoga Park, CA) The KIR genotype of these samples determined by the UCLA KIR Exchange program are summarized in Supplementary Figure A. ..

Polymerase Chain Reaction:

Sequencing:

Transfection:

Article Title: β-Defensin-1 Regulates Influenza Virus Infection in Human Bronchial Epithelial Cells through the STAT3 Signaling Pathway
Article Snippet: .. HBEpC were transfected with miRNA inhibitor oligonucleotide or a mimic oligonucleotide (Thermo Fisher Scientific, Carlsbad, CA, USA) using the lipid-based lipofectamine 2000 reagent diluted in Opti-MEM-I reduced serum medium (Thermo Fisher Scientific) according to the suppliers’ protocol. ..

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