hamster (Bio-Rad)
Structured Review
Hamster, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd81/Hamster+anti+Mouse+CD81/pmc12694724-2-2-4
Average 93 stars, based on 24 article reviews
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Incubation:Article Title: Cooperative role of CD81 with GPR56 and collagen III in cortical formation. Article Snippet: .. Sections were treated with 3 % H2O2, blocked (10 % donkey serum, 1 % BSA, 0.1 % Triton X-100), and incubated with Expressing:Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g . These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development. Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g. These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), Marker:Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g . These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development. Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g. These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), Negative Control:Article Title: Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways Article Snippet: Following electrophoresis, proteins were transferred onto nitrocellulose membranes using iBlot2 (Invitrogen) and blocked in 5% non-fat dry milk in TBST. .. The following primary antibodies were used: Alix (C-11, sc-271975, Santa Cruz Biotechnology, USA), Annexin II (ab214486, Abcam, UK), Article Title: Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways Article Snippet: Following electrophoresis, proteins were transferred onto nitrocellulose membranes using iBlot2 (Invitrogen) and blocked in 5% non-fat dry milk in TBST. .. The following primary antibodies were used: Alix (C-11, sc-271975, Santa Cruz Biotechnology, USA), Annexin II (ab214486, Abcam, UK), |
