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hamster  (Bio-Rad)


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  • 93

    Structured Review

    Bio-Rad hamster
    Hamster, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd81/Hamster+anti+Mouse+CD81/pmc12694724-2-2-4
    Average 93 stars, based on 24 article reviews
    hamster - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Cooperative role of CD81 with GPR56 and collagen III in cortical formation.
    Article Snippet: .. Sections were treated with 3 % H2O2, blocked (10 % donkey serum, 1 % BSA, 0.1 % Triton X-100), and incubated with hamster anti-CD81 antibodies (BioRad; 1:300). .. Signals were amplified using a TSA-TMR kit (PerkinElmer) following incubation with HRP-conjugated anti-hamster IgG.

    Expressing:

    Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development
    Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g . These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), CD81 (MCA1846, Bio-Rad Laboratories, Inc., Hercules, CA, USA), CD63 (BD551458, BD Biosciences, Franklin Lakes, NJ, USA), HSP90 (C45G5, Cell Signaling Technology, DANVERS, MA, USA), and negative marker GM130 (BD610822, BD Biosciences, Franklin Lakes, NJ, USA), as described in previous papers [ , , ]. .. Briefly, 25–30 μg of BDEV was subjected to Western blot under reducing (Hsp70, Flot-1, GM130) and non-reducing (CD81, CD63, Hsp90) conditions using 4–12% Bis-tris gels (Invitrogen, Waltham, MA, USA).

    Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development.
    Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g. These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), CD81 (MCA1846, Bio-Rad Laboratories, Inc., Hercules, CA, USA), CD63 (BD551458, BD Biosciences, Franklin Lakes, NJ, USA), HSP90 (C45G5, Cell Signaling Technology, DANVERS, MA, USA), and negative marker GM130 (BD610822, BD Biosciences, Franklin Lakes, NJ, USA), as described in previous papers [11,14,15]. .. Briefly, 25–30 μg of BDEV was subjected to Western blot under reducing (Hsp70, Flot-1, GM130) and nonreducing (CD81, CD63, Hsp90) conditions using 4–12% Bis-tris gels (Invitrogen, Waltham, MA, USA).

    Marker:

    Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development
    Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g . These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), CD81 (MCA1846, Bio-Rad Laboratories, Inc., Hercules, CA, USA), CD63 (BD551458, BD Biosciences, Franklin Lakes, NJ, USA), HSP90 (C45G5, Cell Signaling Technology, DANVERS, MA, USA), and negative marker GM130 (BD610822, BD Biosciences, Franklin Lakes, NJ, USA), as described in previous papers [ , , ]. .. Briefly, 25–30 μg of BDEV was subjected to Western blot under reducing (Hsp70, Flot-1, GM130) and non-reducing (CD81, CD63, Hsp90) conditions using 4–12% Bis-tris gels (Invitrogen, Waltham, MA, USA).

    Article Title: Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development.
    Article Snippet: To obtain the purified EV pellet, fractions enhanced with EVs were subjected to additional ultracentrifugation at 100,000× g. These EV pellets were then resuspended in particle-free phosphate-buffered saline (PBS), and their protein content was determined using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) per the manufacturer’s manual. .. To establish the purity of the BDEVs, we examined the expression of positive markers: Hsp70 (SAB4200714, Sigma-Aldrich, St. Louis, MO, USA), Flot-1 (abcam41927, Abcam, Cambridge, UK), CD81 (MCA1846, Bio-Rad Laboratories, Inc., Hercules, CA, USA), CD63 (BD551458, BD Biosciences, Franklin Lakes, NJ, USA), HSP90 (C45G5, Cell Signaling Technology, DANVERS, MA, USA), and negative marker GM130 (BD610822, BD Biosciences, Franklin Lakes, NJ, USA), as described in previous papers [11,14,15]. .. Briefly, 25–30 μg of BDEV was subjected to Western blot under reducing (Hsp70, Flot-1, GM130) and nonreducing (CD81, CD63, Hsp90) conditions using 4–12% Bis-tris gels (Invitrogen, Waltham, MA, USA).

    Negative Control:

    Article Title: Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways
    Article Snippet: Following electrophoresis, proteins were transferred onto nitrocellulose membranes using iBlot2 (Invitrogen) and blocked in 5% non-fat dry milk in TBST. .. The following primary antibodies were used: Alix (C-11, sc-271975, Santa Cruz Biotechnology, USA), Annexin II (ab214486, Abcam, UK), CD81 (MCA1846, Bio-Rad Laboratories, Italy), and GM130 (610822, BD Biosciences, USA) as a negative control for cellular contamination. ..

    Article Title: Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways
    Article Snippet: Following electrophoresis, proteins were transferred onto nitrocellulose membranes using iBlot2 (Invitrogen) and blocked in 5% non-fat dry milk in TBST. .. The following primary antibodies were used: Alix (C-11, sc-271975, Santa Cruz Biotechnology, USA), Annexin II (ab214486, Abcam, UK), CD81 (MCA1846, Bio-Rad Laboratories, Italy), and GM130 (610822, BD Biosciences, USA) as a negative control for cellular contamination. ..



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    miR-1290 is upregulated in breast cancer and BCAF/MB-231-derived exosomes. a Nanoparticle tracking analysis of exosomes isolated from BCAFs and MB-231 cells showing their size distribution. The mean size and standard deviation (SD) are indicated for each sample. b TEM images of exosomes isolated from BCAFs (upper) and MB-231 cells (lower) showing the characteristic morphology of the exosomes. Scale bar, 100 nm. c Western blot analysis of exosome markers (CD63, CD81, and <t>flotillin-1),</t> CAF markers (α-SMA and FAP) and cellular markers (GM130 and H3) in BCAF cells, MB-231 cells and their derived exosomes. d BCAF-derived exosome uptake by TNBC cells. PKH67-labeled BCAF-derived exosomes (green) were incubated with BT-549 and Hs578T cells for 1 hour. The cell nuclei were stained with Hoechst 33342 (blue). Scale bar, 20 μm. e Heatmap showing miRNA expression profiles in exosomes derived from BCAFs and MB-231 cells. Red indicates high expression, and blue indicates low expression. miR-1290 (highlighted in red) is highly expressed in both exosome types. f Volcano plots showing differentially expressed miRNAs between breast cancer or TNBC samples and normal controls in five independent GEO datasets ( GSE37963 , GSE45666 , GSE45498 , GSE38167 , and GSE61438 ). Red and blue dots represent upregulated and downregulated miRNAs, respectively, selected using fold-change and nominal p value cutoffs (|log2(fold change)| > 0.58 and nominal p < 0.05, two-tailed Welch’s t test). g Venn diagram analysis of upregulated miRNAs across the five GEO datasets revealed four commonly upregulated miRNAs. h Violin plot showing miR-1290 expression levels in serum samples from normal individuals ( N , n = 2686) and breast cancer patients ( P , n = 1280) from the GSE73002 dataset. The expression of miR-1290 was significantly upregulated in the breast cancer patient samples (*** p < 0.001, Mann‒Whitney U test). i Analysis of serum exosomal miR-1290 levels in normal ( N , n = 20) and TNBC patient ( P , n = 39) samples collected at Keimyung University Dongsan Hospital. The results support the potential of miR-1290 as a candidate diagnostic marker in an independent cohort (*** p < 0.001, Mann‒Whitney U test). j Kaplan‒Meier plot of overall survival for TNBC patients based on serum exosomal miR-1290 expression levels from Keimyung University Dongsan Hospital ( p = 0.006, log-rank test)
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    Image Search Results


    miR-1290 is upregulated in breast cancer and BCAF/MB-231-derived exosomes. a Nanoparticle tracking analysis of exosomes isolated from BCAFs and MB-231 cells showing their size distribution. The mean size and standard deviation (SD) are indicated for each sample. b TEM images of exosomes isolated from BCAFs (upper) and MB-231 cells (lower) showing the characteristic morphology of the exosomes. Scale bar, 100 nm. c Western blot analysis of exosome markers (CD63, CD81, and flotillin-1), CAF markers (α-SMA and FAP) and cellular markers (GM130 and H3) in BCAF cells, MB-231 cells and their derived exosomes. d BCAF-derived exosome uptake by TNBC cells. PKH67-labeled BCAF-derived exosomes (green) were incubated with BT-549 and Hs578T cells for 1 hour. The cell nuclei were stained with Hoechst 33342 (blue). Scale bar, 20 μm. e Heatmap showing miRNA expression profiles in exosomes derived from BCAFs and MB-231 cells. Red indicates high expression, and blue indicates low expression. miR-1290 (highlighted in red) is highly expressed in both exosome types. f Volcano plots showing differentially expressed miRNAs between breast cancer or TNBC samples and normal controls in five independent GEO datasets ( GSE37963 , GSE45666 , GSE45498 , GSE38167 , and GSE61438 ). Red and blue dots represent upregulated and downregulated miRNAs, respectively, selected using fold-change and nominal p value cutoffs (|log2(fold change)| > 0.58 and nominal p < 0.05, two-tailed Welch’s t test). g Venn diagram analysis of upregulated miRNAs across the five GEO datasets revealed four commonly upregulated miRNAs. h Violin plot showing miR-1290 expression levels in serum samples from normal individuals ( N , n = 2686) and breast cancer patients ( P , n = 1280) from the GSE73002 dataset. The expression of miR-1290 was significantly upregulated in the breast cancer patient samples (*** p < 0.001, Mann‒Whitney U test). i Analysis of serum exosomal miR-1290 levels in normal ( N , n = 20) and TNBC patient ( P , n = 39) samples collected at Keimyung University Dongsan Hospital. The results support the potential of miR-1290 as a candidate diagnostic marker in an independent cohort (*** p < 0.001, Mann‒Whitney U test). j Kaplan‒Meier plot of overall survival for TNBC patients based on serum exosomal miR-1290 expression levels from Keimyung University Dongsan Hospital ( p = 0.006, log-rank test)

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Breast cancer-associated fibroblast-derived exosomal miR-1290 promotes triple-negative breast cancer cell proliferation

    doi: 10.1038/s41392-026-02922-y

    Figure Lengend Snippet: miR-1290 is upregulated in breast cancer and BCAF/MB-231-derived exosomes. a Nanoparticle tracking analysis of exosomes isolated from BCAFs and MB-231 cells showing their size distribution. The mean size and standard deviation (SD) are indicated for each sample. b TEM images of exosomes isolated from BCAFs (upper) and MB-231 cells (lower) showing the characteristic morphology of the exosomes. Scale bar, 100 nm. c Western blot analysis of exosome markers (CD63, CD81, and flotillin-1), CAF markers (α-SMA and FAP) and cellular markers (GM130 and H3) in BCAF cells, MB-231 cells and their derived exosomes. d BCAF-derived exosome uptake by TNBC cells. PKH67-labeled BCAF-derived exosomes (green) were incubated with BT-549 and Hs578T cells for 1 hour. The cell nuclei were stained with Hoechst 33342 (blue). Scale bar, 20 μm. e Heatmap showing miRNA expression profiles in exosomes derived from BCAFs and MB-231 cells. Red indicates high expression, and blue indicates low expression. miR-1290 (highlighted in red) is highly expressed in both exosome types. f Volcano plots showing differentially expressed miRNAs between breast cancer or TNBC samples and normal controls in five independent GEO datasets ( GSE37963 , GSE45666 , GSE45498 , GSE38167 , and GSE61438 ). Red and blue dots represent upregulated and downregulated miRNAs, respectively, selected using fold-change and nominal p value cutoffs (|log2(fold change)| > 0.58 and nominal p < 0.05, two-tailed Welch’s t test). g Venn diagram analysis of upregulated miRNAs across the five GEO datasets revealed four commonly upregulated miRNAs. h Violin plot showing miR-1290 expression levels in serum samples from normal individuals ( N , n = 2686) and breast cancer patients ( P , n = 1280) from the GSE73002 dataset. The expression of miR-1290 was significantly upregulated in the breast cancer patient samples (*** p < 0.001, Mann‒Whitney U test). i Analysis of serum exosomal miR-1290 levels in normal ( N , n = 20) and TNBC patient ( P , n = 39) samples collected at Keimyung University Dongsan Hospital. The results support the potential of miR-1290 as a candidate diagnostic marker in an independent cohort (*** p < 0.001, Mann‒Whitney U test). j Kaplan‒Meier plot of overall survival for TNBC patients based on serum exosomal miR-1290 expression levels from Keimyung University Dongsan Hospital ( p = 0.006, log-rank test)

    Article Snippet: Membranes were incubated with the following primary antibodies: CD63 (1:200; ABclonal, A5271), CD81 (1:200; ABclonal, A5270), flotillin-1 (1:1000; ABclonal, A6220), α-SMA (1:1000; ABclonal, A17910), FAP (1:1000; ABclonal, A23789 ), GM130 (1:1000; Abcam, ab52649), H3 (1:1000; Cell Signaling Technology, 4620S), ACTB (1:20,000; ABclonal, AC026), TBC1D4 (1:200; Cell Signaling Technology, 2670S), P70S6K (1:500; Cell Signaling Technology, 9202S), phospho-P70S6K (1:500; ABclonal, AP0564), and Ki67 (1:1000; Abcam, ab16667).

    Techniques: Derivative Assay, Exosomes, Analysis, Isolation, Standard Deviation, Morphology, Western Blot, Labeling, Incubation, Staining, Expressing, Two Tailed Test, Blood/Plasma/Serum Samples, Diagnostic Assay, Marker