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anti cd80  (Bio X Cell)


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    Bio X Cell anti cd80
    Anti Cd80, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd80/InVivoMAb+anti-mouse+CD80/pmc11228532-119-4-7
    Average 93 stars, based on 14 article reviews
    anti cd80 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: PD-1 or CTLA-4 blockade promotes CD86-driven Treg responses upon radiotherapy of lymphocyte-depleted cancer in mice
    Article Snippet: For Treg depletion experiments, mice were injected i.p. with 250 μg depleting mouse IgG2a isotype CD25 mAbs ( ) (modified clone of PC61, Evitria) in 100 μL PBS on day –1 prior to RT and on day 5. .. Blocking mAbs against CTLA-4 (UC10-4F10-11, Bio X Cell), PD-1 (RMP1-14, Bio X Cell), CD80 (1G10, Bio X Cell), and CD86 (GL-1, Bio X Cell) were injected i.p. at either 100 μg (anti–CTLA-4 and anti–PD-1) or 200 μg (anti-CD80 and anti-CD86) per mouse in 100 μL PBS on the day of RT (day 0) and on days 3 and 6 and, in the case of anti–CTLA-4, also on day 9. ..

    Article Title: PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration
    Article Snippet: .. Blocking antibodies against mouse PD-1 (Rmp1-14, BE0146, use at final 5 to 20 ug/ml), mouse PD-L1 (10F.9G2, BE0101, use at final 5-20ug/ml), mouse CD80 (1G10, BE0134, use at final 5 to 20 ug/ml) were purchased from BioXCell; GoInVivo purified anti-human PD-1 (EH12.2H7, 329946, use at final 5 to 20 ug/ml), human PD-L1 (29E.2A3, 329728, use at final 5 to 20 ug/ml), and human PD-L1 (MIH3, 374503, use at final 5 to 20 ug/ml) were purchase from Biolegend. .. All antibodies for flow cytometric analysis were purchased from Biolegend (San Diego, CA) except PE anti-mouse PD-1 (J43, 12-9985-82, 1:400, eBioscience): Alexa Fluor® 647 antihuman PD-1 (EH12.2H7, 329910, 1:300); APC anti-mouse PD-L1 (10F.9G2, 124312, 1:400); Brilliant Violet 421TM anti-human PD-L1 (29E.2A3, 329713, 1:300); FITC anti-mouse CD80 (16-10A1, 104706, 1:300); PE anti-human CD80 (2D10, 305208, 1:300); APC antimouse VCAM-1 (429, 105718, 1:100); Brilliant Violet 421TM (RM4-5, 100544, 1:300) or PerCP/Cyanine5.5 anti-mouse CD4 (RM4-5, 100540, 1:400); APC anti-mouse CD25 (PC61, 102012, 1:500); PE anti-mouse Foxp3 (MF-14, 126404, 1:200); APC anti-mouse IFN-y (XMG1.2, 505810, 1:200); FITC anti-T-bet (4B10, 644812,1:200); APC anti-mouse Ki-67 (16A8, 652406, 1:400); PerCP/Cyanine5.5 antimouse CD8 (53-6.7, 100734, 1:400); APC anti-human/mouse Granzyme B (QA16A02, 372204, 1:200); APC anti-mouse TIM-3 (B8.2C12, 134008, 1:400); PE anti-mouse LAG-3 (C9B7W, 125208, 1:400), except for PE anti-mouse Lyve-1 (ALY7, 12-044-382, 1:200, eBioscience).

    Article Title: Monocyte-derived cells promote transient insult-induced brain injury by enhancing CD8 + T cell response.
    Article Snippet: .. In vivo drug/antibody treatments Anti-CD80/86 To block B7-1 and B7-2, 200 μg anti-mouse CD80 (Cat# BE0134, BioXcell) and 200 μg anti-mouse CD86 (Cat# BE0025, BioXcell) were intraperitoneally injected on the first day after gamma-ray irradiation and then every three days for 8 weeks. ..

    Injection:

    Article Title: PD-1 or CTLA-4 blockade promotes CD86-driven Treg responses upon radiotherapy of lymphocyte-depleted cancer in mice
    Article Snippet: For Treg depletion experiments, mice were injected i.p. with 250 μg depleting mouse IgG2a isotype CD25 mAbs ( ) (modified clone of PC61, Evitria) in 100 μL PBS on day –1 prior to RT and on day 5. .. Blocking mAbs against CTLA-4 (UC10-4F10-11, Bio X Cell), PD-1 (RMP1-14, Bio X Cell), CD80 (1G10, Bio X Cell), and CD86 (GL-1, Bio X Cell) were injected i.p. at either 100 μg (anti–CTLA-4 and anti–PD-1) or 200 μg (anti-CD80 and anti-CD86) per mouse in 100 μL PBS on the day of RT (day 0) and on days 3 and 6 and, in the case of anti–CTLA-4, also on day 9. ..

    Article Title: Monocyte-derived cells promote transient insult-induced brain injury by enhancing CD8 + T cell response.
    Article Snippet: .. In vivo drug/antibody treatments Anti-CD80/86 To block B7-1 and B7-2, 200 μg anti-mouse CD80 (Cat# BE0134, BioXcell) and 200 μg anti-mouse CD86 (Cat# BE0025, BioXcell) were intraperitoneally injected on the first day after gamma-ray irradiation and then every three days for 8 weeks. ..

    In Vivo:

    Article Title: A transgenic mouse model for HLA-B*57:01–linked abacavir drug tolerance and reactivity
    Article Snippet: To block PD-1 in vivo, 0.2 mg anti–PD-1 mAb was administered by i.p. injection (clone RPM1-14, BioXcell). .. In vivo neutralization of CD86 and/or CD80 was achieved by treating animals with CTLA-4–Ig (ORENCIA [abatacept], Bristol-Myers Squibb) or anti-CD80 mAb (clone 1G10, BioXcell) as specified in the Supplemental Methods . ..

    Article Title: Monocyte-derived cells promote transient insult-induced brain injury by enhancing CD8 + T cell response.
    Article Snippet: .. In vivo drug/antibody treatments Anti-CD80/86 To block B7-1 and B7-2, 200 μg anti-mouse CD80 (Cat# BE0134, BioXcell) and 200 μg anti-mouse CD86 (Cat# BE0025, BioXcell) were intraperitoneally injected on the first day after gamma-ray irradiation and then every three days for 8 weeks. ..

    Neutralization:

    Article Title: A transgenic mouse model for HLA-B*57:01–linked abacavir drug tolerance and reactivity
    Article Snippet: To block PD-1 in vivo, 0.2 mg anti–PD-1 mAb was administered by i.p. injection (clone RPM1-14, BioXcell). .. In vivo neutralization of CD86 and/or CD80 was achieved by treating animals with CTLA-4–Ig (ORENCIA [abatacept], Bristol-Myers Squibb) or anti-CD80 mAb (clone 1G10, BioXcell) as specified in the Supplemental Methods . ..

    Purification:

    Article Title: PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration
    Article Snippet: .. Blocking antibodies against mouse PD-1 (Rmp1-14, BE0146, use at final 5 to 20 ug/ml), mouse PD-L1 (10F.9G2, BE0101, use at final 5-20ug/ml), mouse CD80 (1G10, BE0134, use at final 5 to 20 ug/ml) were purchased from BioXCell; GoInVivo purified anti-human PD-1 (EH12.2H7, 329946, use at final 5 to 20 ug/ml), human PD-L1 (29E.2A3, 329728, use at final 5 to 20 ug/ml), and human PD-L1 (MIH3, 374503, use at final 5 to 20 ug/ml) were purchase from Biolegend. .. All antibodies for flow cytometric analysis were purchased from Biolegend (San Diego, CA) except PE anti-mouse PD-1 (J43, 12-9985-82, 1:400, eBioscience): Alexa Fluor® 647 antihuman PD-1 (EH12.2H7, 329910, 1:300); APC anti-mouse PD-L1 (10F.9G2, 124312, 1:400); Brilliant Violet 421TM anti-human PD-L1 (29E.2A3, 329713, 1:300); FITC anti-mouse CD80 (16-10A1, 104706, 1:300); PE anti-human CD80 (2D10, 305208, 1:300); APC antimouse VCAM-1 (429, 105718, 1:100); Brilliant Violet 421TM (RM4-5, 100544, 1:300) or PerCP/Cyanine5.5 anti-mouse CD4 (RM4-5, 100540, 1:400); APC anti-mouse CD25 (PC61, 102012, 1:500); PE anti-mouse Foxp3 (MF-14, 126404, 1:200); APC anti-mouse IFN-y (XMG1.2, 505810, 1:200); FITC anti-T-bet (4B10, 644812,1:200); APC anti-mouse Ki-67 (16A8, 652406, 1:400); PerCP/Cyanine5.5 antimouse CD8 (53-6.7, 100734, 1:400); APC anti-human/mouse Granzyme B (QA16A02, 372204, 1:200); APC anti-mouse TIM-3 (B8.2C12, 134008, 1:400); PE anti-mouse LAG-3 (C9B7W, 125208, 1:400), except for PE anti-mouse Lyve-1 (ALY7, 12-044-382, 1:200, eBioscience).

    Article Title: PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration
    Article Snippet: .. Functional grade purified antibodies against mouse PD-1 (Rmp1-14), mouse PD-L1 (10 F.9G2), mouse CD80 (1G10) were purchased from BioXCell; F(ab’)2 and Fab Abs were prepared with Pierce TM F(ab’)2 Micro preparation kit (#44688) and Pierce TM Fab Micro preparation kit (#44685), respectively, the fragmented Abs were further purified and desalted with AminoLink Immobilization Kit (#43426) and polyacrylamide desalting columns (#43426). ..

    Mouse Assay:

    Article Title: Sustained Type I interferon signaling as a mechanism of resistance to PD-1 blockade
    Article Snippet: In parallel, cells were harvested, washed and pre-incubated with anti-CD16/32 Ab (clone 93, eBioscience) for 20 min at 4 °C and then stained to discriminate populations and markers of interest using the antibodies listed in Supplementary information, Table S . In vivo treatments . .. Mice were treated intraperitoneally (i.p.) when tumors became palpable (20 to 25 mm2) with anti-PD-1 mAb (250 μg/mouse; clone RMP1–14, BioXcell, NH, USA), with or without anti-CD80 (500 μg/mouse; clone 1G10, BioXcell, NH, USA), anti-PD-L1 (250 μg/mouse; clone 10 F.9G2, BioXcell, NH, USA), anti-CTLA-4 (100 μg/mouse; clone 9D9, BioXcell, NH, USA) (or the appropriate isotype control) mAb as described in the figures or figure legends. ..

    Control:

    Article Title: Sustained Type I interferon signaling as a mechanism of resistance to PD-1 blockade
    Article Snippet: In parallel, cells were harvested, washed and pre-incubated with anti-CD16/32 Ab (clone 93, eBioscience) for 20 min at 4 °C and then stained to discriminate populations and markers of interest using the antibodies listed in Supplementary information, Table S . In vivo treatments . .. Mice were treated intraperitoneally (i.p.) when tumors became palpable (20 to 25 mm2) with anti-PD-1 mAb (250 μg/mouse; clone RMP1–14, BioXcell, NH, USA), with or without anti-CD80 (500 μg/mouse; clone 1G10, BioXcell, NH, USA), anti-PD-L1 (250 μg/mouse; clone 10 F.9G2, BioXcell, NH, USA), anti-CTLA-4 (100 μg/mouse; clone 9D9, BioXcell, NH, USA) (or the appropriate isotype control) mAb as described in the figures or figure legends. ..

    Functional Assay:

    Article Title: PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration
    Article Snippet: .. Functional grade purified antibodies against mouse PD-1 (Rmp1-14), mouse PD-L1 (10 F.9G2), mouse CD80 (1G10) were purchased from BioXCell; F(ab’)2 and Fab Abs were prepared with Pierce TM F(ab’)2 Micro preparation kit (#44688) and Pierce TM Fab Micro preparation kit (#44685), respectively, the fragmented Abs were further purified and desalted with AminoLink Immobilization Kit (#43426) and polyacrylamide desalting columns (#43426). ..

    Irradiation:

    Article Title: Monocyte-derived cells promote transient insult-induced brain injury by enhancing CD8 + T cell response.
    Article Snippet: .. In vivo drug/antibody treatments Anti-CD80/86 To block B7-1 and B7-2, 200 μg anti-mouse CD80 (Cat# BE0134, BioXcell) and 200 μg anti-mouse CD86 (Cat# BE0025, BioXcell) were intraperitoneally injected on the first day after gamma-ray irradiation and then every three days for 8 weeks. ..



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    Image Search Results


    Proposed model illustrating the effects of EZH2 inhibition across multiple joint compartments in osteoarthritis. In OA fibroblast-like synoviocytes (FLS), increased EZH2 and H3K27me3 levels were associated with inflammatory and catabolic responses, which were reduced by pharmacological EZH2 inhibition with EPZ-6438. In human OA FLS, EPZ-6438 decreased inflammatory, catabolic and pain-associated mediators, including IL1B, MMP13, NGF and NTN1, reduced LDHA expression and increased autophagy-associated responses. Proteomic and ChIP-seq analyses further identified inflammatory, metabolic and axon guidance-related pathways, including inflammation-dependent EZH2 recruitment at the PAX6 locus. EZH2 inhibition also reduced inflammatory activation of macrophages and osteoclastogenesis in OA-derived bone marrow cells. Collectively, these findings support a broader role for EZH2 in inflammatory, catabolic and osteoimmune responses across multiple OA-relevant cellular compartments. The identification of PAX6, NTN1 and axon guidance-related pathways highlights neuro-associated molecular programs that may be relevant to pain-associated responses, although their direct contribution to pain or sensory nerve remodeling was not assessed in this study. The reduced structural joint damage and pain-related behaviors observed following EZH2 inhibition in the MIA-induced OA model are consistent with this multicellular framework

    Journal: Molecular Biomedicine

    Article Title: EZH2 coordinates inflammatory and pain-associated pathways across joint compartments in osteoarthritis

    doi: 10.1186/s43556-026-00643-w

    Figure Lengend Snippet: Proposed model illustrating the effects of EZH2 inhibition across multiple joint compartments in osteoarthritis. In OA fibroblast-like synoviocytes (FLS), increased EZH2 and H3K27me3 levels were associated with inflammatory and catabolic responses, which were reduced by pharmacological EZH2 inhibition with EPZ-6438. In human OA FLS, EPZ-6438 decreased inflammatory, catabolic and pain-associated mediators, including IL1B, MMP13, NGF and NTN1, reduced LDHA expression and increased autophagy-associated responses. Proteomic and ChIP-seq analyses further identified inflammatory, metabolic and axon guidance-related pathways, including inflammation-dependent EZH2 recruitment at the PAX6 locus. EZH2 inhibition also reduced inflammatory activation of macrophages and osteoclastogenesis in OA-derived bone marrow cells. Collectively, these findings support a broader role for EZH2 in inflammatory, catabolic and osteoimmune responses across multiple OA-relevant cellular compartments. The identification of PAX6, NTN1 and axon guidance-related pathways highlights neuro-associated molecular programs that may be relevant to pain-associated responses, although their direct contribution to pain or sensory nerve remodeling was not assessed in this study. The reduced structural joint damage and pain-related behaviors observed following EZH2 inhibition in the MIA-induced OA model are consistent with this multicellular framework

    Article Snippet: The following antibodies were used for immune cell characterization: CD11b (REA592 VioBlue, 130–113–810, Miltenyi Biotec), F4/80 (REA126 PE-Vio770, 130–118–459, Miltenyi Biotec), CD80 (REA983 APC-Vio770, 130–116–463, Miltenyi Biotec), CD86 (REA1190 PE, 130–122–129, Miltenyi Biotec), MHC-II (REA813 FITC, 130–112–386, Miltenyi Biotec), CD38 (REA616 PerCP-Vio700, 130–109–260, Miltenyi Biotec), and iNOS (REA982 APC, 130–116–423, Miltenyi Biotec).

    Techniques: Inhibition, Expressing, ChIP-sequencing, Activation Assay, Derivative Assay