Journal:

Article Title: CTLA-4 regulates allergen response by modulating GATA-3 protein level per cell

doi: 10.1111/j.1365-2567.2007.02537.x

Figure Lengend Snippet: Effects of in vitro CTLA-4 blockade on GATA-3 protein level/cell and frequency of IL-4-producing cells. Naive CD4+ T cells purified from the spleens of normal mice were induced to differentiate with anti-CD3 mAb, a recombinant form of CD80-Fc, IL-4 and anti-IFN-γ mAb for 3 days. Anti-CTLA-4 mAb was added in soluble form at increasing concentrations (0·1–10 μg/ml). (a) Mean fluorescence intensity for GATA-3 staining, (b) percentage of IL-4+ cells.

Article Snippet: 18 CD4 cell culture For in vitro experiments, splenic naive CD4 cells were purified from non-immunized BALB/c mice by immuno-magnetic cell sorting as previously described 19 and cultured in 24-well plates precoated with anti-CD3ε mAb (clone 145-2C11; 10 μg/ml) and/or recombinant mouse B7-1(CD80)/Fc chimeric protein (R & D Systems, cat. 740-B1; 0·33 μg/ml).

Techniques: In Vitro, Purification, Recombinant, Fluorescence, Staining

Journal: Arthritis Research & Therapy

Article Title: Interferon-induced protein IFIT4 is associated with systemic lupus erythematosus and promotes differentiation of monocytes into dendritic cell-like cells

doi: 10.1186/ar2475

Figure Lengend Snippet: Comparison of the phenotypic profiles of DCLCs primed with or without IFIT4 over-expression. (a) Surface antigens of normal THP-1 cells were examined by flow cytometry. (b) To analyze the effect of IFIT4 on phenotypic changes of dendritic cell-like cells (DCLCs) during the process of differentiation, THP-1 cells were transfected with pEGFP-IFIT4 or pEGFP-C1; 36 hours later, cells were stimulated with granulocyte-macrophage colony-stimulating factor (GM-CSF; 50 ng/ml) and IL-4 (20 ng/ml) for 90 hours to generate DCLCs. These DCLCs primed with pEGFP-IFIT4 or pEGFP-C1 transfection were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) and the antigens of CD40, CD80, CD86, CD83, HLA-DR, CD14 and CD1a on the surface of those DCLCs were analyzed by flow cytometry. Appropriate fluorochrome or isotype control mAbs of each antibody species were used as negative controls. Shaded histograms represent isotype control antibodies. The thick line represents DCLCs primed with pEGFP-IFIT4 transfection, whereas the slender lines represent DCLCs primed with pEGFP-C1 transfection. All experiments were performed three times and a set of representative histograms was presented. IFIT4, interferon-induced protein with tetratricopeptide repeats 4.

Article Snippet: FITC anti-human CD40 (Catalogue no: FAB617F) and PE anti-human CD80 (Catalogue no: FAB140P) were from R&D Systems, Minneapolis, MN, USA.

Techniques: Comparison, Over Expression, Flow Cytometry, Transfection, Incubation, Bioprocessing, Control

Journal: Frontiers in Immunology

Article Title: Impact of alcohol consumption on outcomes and potential of immune biomarkers for postoperative complications in trauma patients

doi: 10.3389/fimmu.2025.1492288

Figure Lengend Snippet: Comparison of patient demographics, comorbidities, and postoperative complications between alcohol-negative and alcohol-positive groups included in the ELISA analysis.

Article Snippet: ELISA kits for CD28 (#DY342-05), B7-1 (#DY140) (R&D Systems, Minneapolis, MN, USA), Interleukin 13 (IL-13; #900-K23), Eotaxin-3 (#900-K167), and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1; #900-K438) (PeproTech, Hamburg, Germany) were used according to the manufacturer’s protocols.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Control, Infection

Journal: Frontiers in Immunology

Article Title: Impact of alcohol consumption on outcomes and potential of immune biomarkers for postoperative complications in trauma patients

doi: 10.3389/fimmu.2025.1492288

Figure Lengend Snippet: ELISA quantification of key inflammatory and T/B cell activation markers in patient serum samples. Patients were categorized as negative for alcohol risk (Neg.) or positive for alcohol risk (Pos.). Each group is subdivided into a control group (Ctr.) and a complication group (Comp.). The levels of (A) CD28, (B) B7-1, (C) Interleukin 13 (IL-13), (D) Eotaxin-3, and (E) Tissue Inhibitor of Metalloproteinase 1 (TIMP-1). The data presented as Tukey’s boxplots of the 4 groups Neg. Ctr. (N=31), Neg. Comp. (N=17), Pos. Ctr. (N=46), and Pos. Comp. (N=16). Statistical analysis was performed using 2-way ANOVA followed by Tukey’s multiple comparisons test if the effect of alcohol and/or complication was significant, with significance indicated by *p < 0.05.

Article Snippet: ELISA kits for CD28 (#DY342-05), B7-1 (#DY140) (R&D Systems, Minneapolis, MN, USA), Interleukin 13 (IL-13; #900-K23), Eotaxin-3 (#900-K167), and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1; #900-K438) (PeproTech, Hamburg, Germany) were used according to the manufacturer’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay, Activation Assay, Control