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cd59a  (Miltenyi Biotec)


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    Miltenyi Biotec cd59a
    Cd59a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd59a/CD59+Antibody%2C+anti-rat%2C+REAfinity/pmc12751069-46-36-38
    Average 93 stars, based on 2 article reviews
    cd59a - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Anti-HIV-1 antibodies trigger non-lytic complement deposition on infected cells.
    Article Snippet: .. Seven days after nucleofection, a 2019 Institut Pasteur EMBO reports 21: e49351 | 2020 13 of 16 knockout efficacy was evaluated by flow cytometry using an antiCD59 antibody (Miltenyi, final dilution 1:50). .. Data processing and statistical analysis Calculations were performed and figures were drawn using Excel 365 or GraphPad Prism 8.0.

    Flow Cytometry:

    Article Title: Anti-HIV-1 antibodies trigger non-lytic complement deposition on infected cells.
    Article Snippet: .. Seven days after nucleofection, a 2019 Institut Pasteur EMBO reports 21: e49351 | 2020 13 of 16 knockout efficacy was evaluated by flow cytometry using an antiCD59 antibody (Miltenyi, final dilution 1:50). .. Data processing and statistical analysis Calculations were performed and figures were drawn using Excel 365 or GraphPad Prism 8.0.

    Staining:

    Article Title: Maternal dietary fibre intake results in sex-specific single-cell molecular changes in the heart of the offspring
    Article Snippet: .. Cells were then stained with an antibody cocktail including: I-A/I-E (2G9, BD Biosciences), CD11b (M1/70, BD Biosciences), CD64 (a & b alloantigens, X54-5/7.1, BD Biosciences), CD146 (ME-9F1, BD Biosciences), CD31 (390, BD Biosciences), Ly6C (HK1.4, Biolegend), CD59a (REA287, Miltenyi Biotec), Ly6G (1A8, Biolegend), NK1.1 (PK136, Biolegend), CD39 (Duha59, Biolegend), CD90.2 (30-H12, BD Biosciences) and CD45 (30-F11, BD Biosciences). .. SYTOXTM Green Dead Cell Stain ( S34860 , Invitrogen) and eBioscienceTM Calcein Blue AM Viability Dye (65–0855-39, Invitrogen) were used to identify live and metabolically active cells.



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    Image Search Results


    Gene expression and cytogenetic clusters in MM. (A) Violin plots showing gene expression levels for TNFRSF13B , CD59 , FCGR2B , SLC44A1 , and CD320 in HD, MGUS, SMM, MM, RRMM. Statistical comparisons were conducted using the Kruskal–Wallis test for all pairwise contrasts. (B) Heatmap of the pseudobulk expression profiles showing normalized expression levels for eight putative proxy markers of cytogenetic aberrations across 54 MM patients. (C) Box plots illustrating the differential expression of TNFRSF13B , CD59 , FCGR2B , SLC44A1 , and CD320 across the four cytogenetic patient clusters. Statistical significance was evaluated using two-sided t -tests for all pairwise comparisons p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).

    Journal: Frontiers in Medicine

    Article Title: Identification of CD320, SLC44A1 and TNFRSF13B as potential novel therapeutic targets for CAR T-cell therapy in multiple myeloma

    doi: 10.3389/fmed.2025.1737919

    Figure Lengend Snippet: Gene expression and cytogenetic clusters in MM. (A) Violin plots showing gene expression levels for TNFRSF13B , CD59 , FCGR2B , SLC44A1 , and CD320 in HD, MGUS, SMM, MM, RRMM. Statistical comparisons were conducted using the Kruskal–Wallis test for all pairwise contrasts. (B) Heatmap of the pseudobulk expression profiles showing normalized expression levels for eight putative proxy markers of cytogenetic aberrations across 54 MM patients. (C) Box plots illustrating the differential expression of TNFRSF13B , CD59 , FCGR2B , SLC44A1 , and CD320 across the four cytogenetic patient clusters. Statistical significance was evaluated using two-sided t -tests for all pairwise comparisons p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).

    Article Snippet: Antibodies included: anti-human CD45 V500 (HI30), anti-human CD19 APC-H7 (HIB19), anti-human CD59 FITC (H19), anti-human CD92 BV605 (VIM15), anti-human CD267 BV421 (1A1-K21-M22) (all from BD Biosciences, USA), anti-human CD38 PE (REA572, Miltenyi Biotec, Germany), and anti-human CD320 AlexaFluor 647 (Antibodies Online, Germany).

    Techniques: Gene Expression, Expressing, Quantitative Proteomics

    Kaplan–Meier survival curves showing the prognostic relevance of mRNA expression levels for CD59, SLC44A1, FCGR2B, TNFRSF13B, and CD320 genes. Cell populations with high expression of each gene are shown in blue, while populations with low expression of the same mRNA are shown in red. Reference datasets (GSE, GEO Series) are indicated at the top of each panel. Prognostic significance was assessed using the Cox proportional hazards model (95% confidence interval CI) and log-rank test, with p < 0.05 considered statistically significant.

    Journal: Frontiers in Medicine

    Article Title: Identification of CD320, SLC44A1 and TNFRSF13B as potential novel therapeutic targets for CAR T-cell therapy in multiple myeloma

    doi: 10.3389/fmed.2025.1737919

    Figure Lengend Snippet: Kaplan–Meier survival curves showing the prognostic relevance of mRNA expression levels for CD59, SLC44A1, FCGR2B, TNFRSF13B, and CD320 genes. Cell populations with high expression of each gene are shown in blue, while populations with low expression of the same mRNA are shown in red. Reference datasets (GSE, GEO Series) are indicated at the top of each panel. Prognostic significance was assessed using the Cox proportional hazards model (95% confidence interval CI) and log-rank test, with p < 0.05 considered statistically significant.

    Article Snippet: Antibodies included: anti-human CD45 V500 (HI30), anti-human CD19 APC-H7 (HIB19), anti-human CD59 FITC (H19), anti-human CD92 BV605 (VIM15), anti-human CD267 BV421 (1A1-K21-M22) (all from BD Biosciences, USA), anti-human CD38 PE (REA572, Miltenyi Biotec, Germany), and anti-human CD320 AlexaFluor 647 (Antibodies Online, Germany).

    Techniques: Expressing

    Histogram plots showing the cell surface expression of CD59, CD92, CD267, and CD320 molecules on different MM cell lines, including AMO-1, OPM-2, RPMI8226, U-266, and H929 as measured by flow cytometry analysis using fluorochrome-conjugated antibodies in triplicate experiments. Each panel represents the distribution of expression levels for the indicated marker, allowing comparison of protein abundance across the different cell lines compared to unstained control (in blue).

    Journal: Frontiers in Medicine

    Article Title: Identification of CD320, SLC44A1 and TNFRSF13B as potential novel therapeutic targets for CAR T-cell therapy in multiple myeloma

    doi: 10.3389/fmed.2025.1737919

    Figure Lengend Snippet: Histogram plots showing the cell surface expression of CD59, CD92, CD267, and CD320 molecules on different MM cell lines, including AMO-1, OPM-2, RPMI8226, U-266, and H929 as measured by flow cytometry analysis using fluorochrome-conjugated antibodies in triplicate experiments. Each panel represents the distribution of expression levels for the indicated marker, allowing comparison of protein abundance across the different cell lines compared to unstained control (in blue).

    Article Snippet: Antibodies included: anti-human CD45 V500 (HI30), anti-human CD19 APC-H7 (HIB19), anti-human CD59 FITC (H19), anti-human CD92 BV605 (VIM15), anti-human CD267 BV421 (1A1-K21-M22) (all from BD Biosciences, USA), anti-human CD38 PE (REA572, Miltenyi Biotec, Germany), and anti-human CD320 AlexaFluor 647 (Antibodies Online, Germany).

    Techniques: Expressing, Flow Cytometry, Marker, Comparison, Quantitative Proteomics, Control

    Histogram plots showing the cell surface expression of CD59, CD92, CD267, and CD320 molecules on malignant plasma cells (PCs) from eight bone marrow samples of newly diagnosed, untreated MM patients as measured by flow cytometry analysis using fluorochrome-conjugated antibodies in triplicate experiments. Each panel represents the distribution of expression levels for the indicated marker across the eight MM patients samples, allowing comparison of protein abundance compared to unstained control (in blue).

    Journal: Frontiers in Medicine

    Article Title: Identification of CD320, SLC44A1 and TNFRSF13B as potential novel therapeutic targets for CAR T-cell therapy in multiple myeloma

    doi: 10.3389/fmed.2025.1737919

    Figure Lengend Snippet: Histogram plots showing the cell surface expression of CD59, CD92, CD267, and CD320 molecules on malignant plasma cells (PCs) from eight bone marrow samples of newly diagnosed, untreated MM patients as measured by flow cytometry analysis using fluorochrome-conjugated antibodies in triplicate experiments. Each panel represents the distribution of expression levels for the indicated marker across the eight MM patients samples, allowing comparison of protein abundance compared to unstained control (in blue).

    Article Snippet: Antibodies included: anti-human CD45 V500 (HI30), anti-human CD19 APC-H7 (HIB19), anti-human CD59 FITC (H19), anti-human CD92 BV605 (VIM15), anti-human CD267 BV421 (1A1-K21-M22) (all from BD Biosciences, USA), anti-human CD38 PE (REA572, Miltenyi Biotec, Germany), and anti-human CD320 AlexaFluor 647 (Antibodies Online, Germany).

    Techniques: Expressing, Clinical Proteomics, Flow Cytometry, Marker, Comparison, Quantitative Proteomics, Control

    The sensitization rate of different group mice.

    Journal: Ecotoxicology and environmental safety

    Article Title: C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice

    doi: 10.1016/j.ecoenv.2022.114020

    Figure Lengend Snippet: The sensitization rate of different group mice.

    Article Snippet: Mouse CD59 protein (Cat. 50724-M08H) was from Sino Biological.

    Techniques: Control