Journal: Journal of xenobiotics

Article Title: Assessment of the In Vitro Phosphatidylinositol Glycan Class A (PIG-A) Gene Mutation Assay Using Human TK6 and Mouse Hepa1c1c7 Cell Lines.

doi: 10.3390/jox14030073

Figure Lengend Snippet: Figure 1. Mutant cell enrichment by separation with anti-CD59 microbeads and paramagnetic separator.

Article Snippet: Anti-mouse PE-conjugated CD59 antibody (Clone REA287, Lot no. 5230105732), anti-human PE-conjugated CD59 antibody (Clone REA496, Lot no. 1322120604), lyophilized anti-PE MicroBeads (Lot no. 5181221156), LS columns (Lot no. 5180927085), and a QuadroMACS separator were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).

Techniques: Mutagenesis

Journal: Journal of xenobiotics

Article Title: Assessment of the In Vitro Phosphatidylinositol Glycan Class A (PIG-A) Gene Mutation Assay Using Human TK6 and Mouse Hepa1c1c7 Cell Lines.

doi: 10.3390/jox14030073

Figure Lengend Snippet: Figure 8. PIG-A gating strategy: (a) gating strategies for separating GPI(−) (lower left region) and GPI(+) (lower right region) of TK6 cell line; (b) gating strategies for separating GPI(−) (lower left region) and GPI(+) (lower right region) of Hepa1c1c7 cell line. PIG-A gene mutated cells were not stained with anti-CD59 antibodies and had no PE signal that was conjugated with antibodies.

Article Snippet: Anti-mouse PE-conjugated CD59 antibody (Clone REA287, Lot no. 5230105732), anti-human PE-conjugated CD59 antibody (Clone REA496, Lot no. 1322120604), lyophilized anti-PE MicroBeads (Lot no. 5181221156), LS columns (Lot no. 5180927085), and a QuadroMACS separator were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).

Techniques: Staining

Journal: PLoS ONE

Article Title: Retinal Pre-Conditioning by CD59a Knockout Protects against Light-Induced Photoreceptor Degeneration

doi: 10.1371/journal.pone.0166348

Figure Lengend Snippet: Photomicrograph showing CD59a immunolabeling in all layers of mouse retina, RPE and choroid (B), but not in CD59aKO eyes (C). Graph of qPCR results showing that mRNA levels of CD59a in NSR were more than 60-fold higher than that in isolated RPE cells in WT eyes (** P <0.001) (D). Data are expressed as means ± SD. N = 4. RPE, retinal pigment epithelium; OS, photoreceptor outer segment; IS, photoreceptor inner segment; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.

Article Snippet: Probes used were cd59a (CD59a, Mm01276239_m1), rhodopsin (Rho, Mm01184405_m1), fibroblast growth factor 2 (Fgf2, Mm00433287_m1), vascular endothelial growth factor (Vegf, Mm01281449_m1), glucose regulated protein 78 (Grp78, Mm00517690_g1), and glucose regulated protein 94 (Grp94, Mm00441926_m1).

Techniques: Immunolabeling, Isolation

Journal: Foods

Article Title: Sea Buckthorn Pericarp Flavonoids Improve Diet-Induced Hyperlipidemia via Coordinated Modulation of Hepatic Lipid Metabolism and Gut Microbiota

doi: 10.3390/foods15061049

Figure Lengend Snippet: TFSP modulates the expression of ACC, FAS, CPT-1α, PPARα and ATGL proteins in the livers of HFD mice. n = 6 per group. Data are presented as mean ± SD. Different letters above bars indicate statistically significant differences ( p < 0.05) by one-way ANOVA followed by LSD post hoc test.

Article Snippet: Peroxisome proliferator-activated receptor alpha (PPARα) primary antibody (Cat. No. A00600-2), carnitine palmitoyltransferase-1 alpha (CPT-1α) primary antibody (Cat. No. A00917-3), acetyl-CoA carboxylase (ACC) primary antibody (Cat. No. M01802-2), fatty acid synthase (FAS) primary antibody (Cat. No. BA0484), adipose triglyceride lipase (ATGL) primary antibody (Cat. No. A01800-1), and GAPDH primary antibody (Cat. No. BM1623) were obtained from BOSTER Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing

Journal: Clinical and Experimental Medicine

Article Title: CD59 silencing enhances doxorubicin sensitivity in DLBCL cells and is associated with poor prognosis in patients treated with CHOP regimen

doi: 10.1007/s10238-026-02134-2

Figure Lengend Snippet: Validation of CD59 knock-down in DLBCL cell lines. ( A ) Representative immunoblots showing CD59 protein levels in SU-DHL-4 and U2932 cell lines transfected with three distinct CD59-shRNA constructs or control vectors. β-actin served as the loading control. ( B and C ) Quantitative analysis of relative CD59 mRNA (by qRT-PCR) and protein (by densitometry) levels in SU-DHL-4 (B) and U2932 (C) cells. Data are presented as mean ± SD and normalized to the blank control group.** p < 0.01; *** p < 0.001

Article Snippet: Anti-CD59 antibody (BM5003), RIPA lysis buffer (AR0105), and protease inhibitor (AR1178) were sourced from Boster Biological Technology Co., Ltd.

Techniques: Biomarker Discovery, Knockdown, Western Blot, Transfection, shRNA, Construct, Control, Quantitative RT-PCR

Journal: Clinical and Experimental Medicine

Article Title: CD59 silencing enhances doxorubicin sensitivity in DLBCL cells and is associated with poor prognosis in patients treated with CHOP regimen

doi: 10.1007/s10238-026-02134-2

Figure Lengend Snippet: CD59 knock-down promotes G0/G1 cell-cycle arrest in SU-DHL-4 and U2932 cells. ( A and B ) Representative propidium-iodide histograms for wild-type and shCD59 transduced SU-DHL-4 (A) and U2932 (B) cells under indicated treatments. ( C ) Bar graph summarizing the proportions of cells in G0/G1, S, and G2/M phases for SU-DHL-4 (left) and U2932 (right) cell lines. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Anti-CD59 antibody (BM5003), RIPA lysis buffer (AR0105), and protease inhibitor (AR1178) were sourced from Boster Biological Technology Co., Ltd.

Techniques: Knockdown

Journal: Clinical and Experimental Medicine

Article Title: CD59 silencing enhances doxorubicin sensitivity in DLBCL cells and is associated with poor prognosis in patients treated with CHOP regimen

doi: 10.1007/s10238-026-02134-2

Figure Lengend Snippet: CD59 knock-down enhances drug-induced apoptosis in DLBCL cell lines. (A-B) Representative Annexin V-PE/7-AAD flow-cytometry plots for wild-type and shCD59 transduced SU-DHL-4 ( A ) and U2932 ( B ) cells under the indicated treatments. ( C ) Bar-graph summarizing the percentage of early + late apoptotic cells. ns p > 0.05, * p < 0.05, ** p < 0.01*** p < 0.001

Article Snippet: Anti-CD59 antibody (BM5003), RIPA lysis buffer (AR0105), and protease inhibitor (AR1178) were sourced from Boster Biological Technology Co., Ltd.

Techniques: Knockdown, Flow Cytometry

Journal: Clinical and Experimental Medicine

Article Title: CD59 silencing enhances doxorubicin sensitivity in DLBCL cells and is associated with poor prognosis in patients treated with CHOP regimen

doi: 10.1007/s10238-026-02134-2

Figure Lengend Snippet: Representative immunohistochemical staining of CD59 in DLBCL tissue specimens. Images show CD59 expression at both 200× (upper panels) and 400× (lower panels) magnification. Representative fields were classified as negative, weakly positive, and positive for CD59 expression. Scale bars: 50 μm (200×) and 20 μm (400×)

Article Snippet: Anti-CD59 antibody (BM5003), RIPA lysis buffer (AR0105), and protease inhibitor (AR1178) were sourced from Boster Biological Technology Co., Ltd.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Clinical and Experimental Medicine

Article Title: CD59 silencing enhances doxorubicin sensitivity in DLBCL cells and is associated with poor prognosis in patients treated with CHOP regimen

doi: 10.1007/s10238-026-02134-2

Figure Lengend Snippet: Progression-free (PFS) and overall survival (OS) analyses based on CD59 expression status in DLBCL. (A and B) Kaplan-Meier curves representing PFS ( A ) and OS ( B ) for 36 CHOP-treated patients stratified by CD59 immunostaining. (C and D) Kaplan-Meier curves representing PFS ( C ) and OS ( D ) for 67 patients who received R-CHOP. (E and F) Kaplan-Meier curves representing PFS ( E ) and OS ( F ) for entire cohort according to CD59 expression. P-values were calculated with the log-rank test

Article Snippet: Anti-CD59 antibody (BM5003), RIPA lysis buffer (AR0105), and protease inhibitor (AR1178) were sourced from Boster Biological Technology Co., Ltd.

Techniques: Expressing, Immunostaining

Journal: Frontiers in Molecular Neuroscience

Article Title: The Alternative Complement System Mediates Cell Death in Retinal Ischemia Reperfusion Injury

doi: 10.3389/fnmol.2018.00278

Figure Lengend Snippet: Complement inhibitors gene expression was down-regulated in the IR retina. The mRNA levels of complement inhibitors, Crry (A,D) , Cd55 (B,E) , and Cd59a (C,F) in the retina of wild type control mice (WT), complement factor b-deficient mice ( Fb −/− ) and complement component 3-deficient mice ( C3 −/− ) at 6 h after IR injury were quantified by RT-PCR, and were normalized to sham surgery. N = 4 eyes/group. Data are presented as the mean ± SEM. * P < 0.05 vs. sham, ** P < 0.01 vs. sham, *** P < 0.001 vs. sham.

Article Snippet: Primers to Crry (Mm00787529_s1, ThermoFischer Scientific), Cd55 (Mm00438377_m1, ThermoFischer Scientific), Cd59a (Mm00483149_m1, ThermoFischer Scientific) were used for mouse retinas, and primers to Cd46 (Hs00611257_m1, ThermoFischer Scientific), Cd55 (Hs00892618_m1, ThermoFischer Scientific), and Cd59 (Hs00174141_m1; ThermoFischer Scientific) were used for HRECs with TaqMan Universal PCR Master Mix (4304437, ThermoFischer Scientific).

Techniques: Gene Expression, Control, Reverse Transcription Polymerase Chain Reaction