Review



recombinant human cd38  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    R&D Systems recombinant human cd38
    (A) Hydrolase activity of <t>recombinant</t> human <t>CD38</t> (rhCD38) in the presence of 78c, using 1,N6-ethenoadenine dinucleotide (ε-NAD+) as substrate (n=3 experiments, IC50 17.7 nM). Inset shows the structure of 78c. R1=H; R2=Me; R3=trans-4- OCH2CH2OMe-cyclohexyl.
    Recombinant Human Cd38, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/Recombinant+Human+CD38+Protein%2C+CF/pmc05935140-53-0-4
    Average 94 stars, based on 16 article reviews
    recombinant human cd38 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline"

    Article Title: A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline

    Journal: Cell metabolism

    doi: 10.1016/j.cmet.2018.03.016

    (A) Hydrolase activity of recombinant human CD38 (rhCD38) in the presence of 78c, using 1,N6-ethenoadenine dinucleotide (ε-NAD+) as substrate (n=3 experiments, IC50 17.7 nM). Inset shows the structure of 78c. R1=H; R2=Me; R3=trans-4- OCH2CH2OMe-cyclohexyl.
    Figure Legend Snippet: (A) Hydrolase activity of recombinant human CD38 (rhCD38) in the presence of 78c, using 1,N6-ethenoadenine dinucleotide (ε-NAD+) as substrate (n=3 experiments, IC50 17.7 nM). Inset shows the structure of 78c. R1=H; R2=Me; R3=trans-4- OCH2CH2OMe-cyclohexyl.

    Techniques Used: Activity Assay, Recombinant

    (A) Immunofluorescent localization of CD38 (red) expression in mouse liver. Sections were co-stained for the pan-leukocyte marker CD45 (green). Hoechst-stained nuclei are shown in blue. Arrow heads (white) indicate lack of CD38 in hepatocytes. Arrows (yellow) show sinusoidal distribution of CD38. Right image depicts a lobular area with a centrally located immune cells cluster. CV= central vein. Scale bar represents 50 μm.
    Figure Legend Snippet: (A) Immunofluorescent localization of CD38 (red) expression in mouse liver. Sections were co-stained for the pan-leukocyte marker CD45 (green). Hoechst-stained nuclei are shown in blue. Arrow heads (white) indicate lack of CD38 in hepatocytes. Arrows (yellow) show sinusoidal distribution of CD38. Right image depicts a lobular area with a centrally located immune cells cluster. CV= central vein. Scale bar represents 50 μm.

    Techniques Used: Expressing, Staining, Marker

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Control, Recombinant, Transfection, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Activity Assay, Colorimetric Assay, Reverse Transcription, Luminescence Assay, Derivative Assay, Plasmid Preparation, Modification, Clone Assay, Mutagenesis, Construct, Software

     CD38  E230Q PCR Assays
    Figure Legend Snippet: CD38 E230Q PCR Assays

    Techniques Used:

    TaqMan Gene Expression Assays
    Figure Legend Snippet: TaqMan Gene Expression Assays

    Techniques Used: Gene Expression

    Related Articles

    Recombinant:

    Article Title: BST1 regulates nicotinamide riboside metabolism via its glycohydrolase and base-exchange activities
    Article Snippet: Individual chromatograms were extracted by MassHunter Workstation-Qualitative Analysis (Version B.05.00, Agilent technologies, Santa Clara, CA, USA). .. Recombinant human BST1 and CD38 proteins were purchased from R&D systems (Minneapolis, MN, USA). rBST1 or rCD38 protein (1 ng for NAD + and NMN degradation, 20 ng for NR degradation) were incubated with 0.1 mM NR, 0.2 mM NAD + , or 0.2 mM NMN in 10 μl of reaction buffer containing 25 mM Tris-HCl, pH 7.4 at 37 °C for 10 and 30 min. ..

    Article Title: Methods and materials for assessing response to plasmablast- and plasma cell-depleting therapies
    Article Snippet: The unique scFV clones were then ranked based on off-rates determined by Biacore® analysis. .. 200RU to 500RU of human recombinant CD38 (R&D Systems' cat #2404-AC or equivalent) were immobilized by standard amine coupling chemistry (Biacore®) to a CM5 or equivalent chip. ..

    Incubation:

    Article Title: BST1 regulates nicotinamide riboside metabolism via its glycohydrolase and base-exchange activities
    Article Snippet: Individual chromatograms were extracted by MassHunter Workstation-Qualitative Analysis (Version B.05.00, Agilent technologies, Santa Clara, CA, USA). .. Recombinant human BST1 and CD38 proteins were purchased from R&D systems (Minneapolis, MN, USA). rBST1 or rCD38 protein (1 ng for NAD + and NMN degradation, 20 ng for NR degradation) were incubated with 0.1 mM NR, 0.2 mM NAD + , or 0.2 mM NMN in 10 μl of reaction buffer containing 25 mM Tris-HCl, pH 7.4 at 37 °C for 10 and 30 min. ..

    Concentration Assay:

    Article Title: CD38‐Specific Gallium‐68 Labeled Peptide Radiotracer Enables Pharmacodynamic Monitoring in Multiple Myeloma with PET
    Article Snippet: The experiments were conducted using a Biacore T200 instrument with a CM5 chip at 25 °C. .. The ligands used were His‐Tagged human CD38 (R&D systems, catalog # 2404‐AC, 43 kDa, 0.5 mg mL −1 stock concentration) and mouse CD38 proteins (R&D systems, catalog # 4947‐AC, 40 kDa, 1.81 mg mL −1 stock concentration), which were immobilized onto the CM5 chip. ..

    Article Title: A GALLIUM-68-LABELED PEPTIDE RADIOTRACER FOR CD38-TARGETED IMAGING IN MULTIPLE MYELOMA WITH PET
    Article Snippet: The experiments were conducted using a Biacore T200 instrument with a CM5 chip at 25 °C. .. The ligands used were His-Tagged human CD38 (R&D systems, catalog # 2404-AC, 43 kDa, 0.5 mg/ml stock concentration) and mouse CD38 proteins (R&D systems, catalog # 4947-AC, 40 kDa, 1.81 mg/ml stock concentration), which were immobilized onto the CM5 chip. ..

    Article Title: CD38-Specific Gallium-68 Labeled Peptide Radiotracer Enables Pharmacodynamic Monitoring in Multiple Myeloma with PET.
    Article Snippet: The limited availability of molecularly targeted low-molecular-weight imaging agents for monitoring multiple myeloma (MM)-targeted therapies has been a significant challenge in the field.. In response, a first-in-class peptide-based radiotracer, [68Ga]Ga-AJ206, is developed that can be seamlessly integrated into the standard clinical workflow and is specifically designed to noninvasively quantify CD38 levels and pharmacodynamics by positron emission tomography (PET).. A bicyclic peptide, AJ206, is synthesized and exhibits high affinity to CD38 (KD: 19.1 ± 0.99 × 10−9 m) by surface plasmon resonance.

    Sample Prep:

    Article Title: Compositions, methods and/or kits comprising a recombinant human CD38- extracellular domain
    Article Snippet: .. Sample preparation (inhibition test): 25 μl of anti-CD38 spiked donor plasma were mixed with 2 μl of 33.4 mg/mL CD38ecd-Fc10H or with 0.447 mg/ml of rhCD38 (CD38-6H, R&D Systems, cat #2404-AC-010, lot. .. PEH0417081, Minneapolis, USA) or with PBS, pH 7.4 and incubated for 15 min at 37° C. Antibody Screening: 50 μl of Screen-Cyte 0.8% Cells #1-3, lot 18001 (Medion Grifols Diagnostics, Duedingen, Switzerland) and 25 μl of the pre-treated simulated plasma were pipetted in the incubation chamber of a microcolumn in a DG Gel Coombs Card (lot 17108.01 exp 2018-09; Diagnostic Grifols, Barcelona, Spain) and incubated for 15 min at 37° C. The Card was then centrifuged in a centrifuge for DG Gel cards and the results read.

    Inhibition:

    Article Title: Compositions, methods and/or kits comprising a recombinant human CD38- extracellular domain
    Article Snippet: .. Sample preparation (inhibition test): 25 μl of anti-CD38 spiked donor plasma were mixed with 2 μl of 33.4 mg/mL CD38ecd-Fc10H or with 0.447 mg/ml of rhCD38 (CD38-6H, R&D Systems, cat #2404-AC-010, lot. .. PEH0417081, Minneapolis, USA) or with PBS, pH 7.4 and incubated for 15 min at 37° C. Antibody Screening: 50 μl of Screen-Cyte 0.8% Cells #1-3, lot 18001 (Medion Grifols Diagnostics, Duedingen, Switzerland) and 25 μl of the pre-treated simulated plasma were pipetted in the incubation chamber of a microcolumn in a DG Gel Coombs Card (lot 17108.01 exp 2018-09; Diagnostic Grifols, Barcelona, Spain) and incubated for 15 min at 37° C. The Card was then centrifuged in a centrifuge for DG Gel cards and the results read.

    Clinical Proteomics:

    Article Title: Compositions, methods and/or kits comprising a recombinant human CD38- extracellular domain
    Article Snippet: .. Sample preparation (inhibition test): 25 μl of anti-CD38 spiked donor plasma were mixed with 2 μl of 33.4 mg/mL CD38ecd-Fc10H or with 0.447 mg/ml of rhCD38 (CD38-6H, R&D Systems, cat #2404-AC-010, lot. .. PEH0417081, Minneapolis, USA) or with PBS, pH 7.4 and incubated for 15 min at 37° C. Antibody Screening: 50 μl of Screen-Cyte 0.8% Cells #1-3, lot 18001 (Medion Grifols Diagnostics, Duedingen, Switzerland) and 25 μl of the pre-treated simulated plasma were pipetted in the incubation chamber of a microcolumn in a DG Gel Coombs Card (lot 17108.01 exp 2018-09; Diagnostic Grifols, Barcelona, Spain) and incubated for 15 min at 37° C. The Card was then centrifuged in a centrifuge for DG Gel cards and the results read.



    Similar Products

    94
    MedChemExpress 78c
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    78c, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+inhibitor+1/pmc13669683-309-7-8
    Average 94 stars, based on 1 article reviews
    78c - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    Miltenyi Biotec anti mouse cd38 pe vio770
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Anti Mouse Cd38 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+Antibody%2C+anti-mouse/pmc13337242-96-3-7
    Average 93 stars, based on 1 article reviews
    anti mouse cd38 pe vio770 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    MedChemExpress cd38 inhibitor 78c
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Cd38 Inhibitor 78c, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+inhibitor+1/pm42814413-242-21-26
    Average 94 stars, based on 1 article reviews
    cd38 inhibitor 78c - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    MedChemExpress anti cd38 antibody
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Anti Cd38 Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+Antibody/pm42814413-313-4-7
    Average 93 stars, based on 1 article reviews
    anti cd38 antibody - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Proteintech anti cd38 antibody
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Anti Cd38 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+Antibody/pm42814413-315-14-17
    Average 94 stars, based on 1 article reviews
    anti cd38 antibody - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Proteintech anti cd38
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Anti Cd38, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+Antibody/pm42775811-424-64-67
    Average 94 stars, based on 1 article reviews
    anti cd38 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    MedChemExpress cd38 inhibitor 1
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Cd38 Inhibitor 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/CD38+inhibitor+1/10__1186_slash_s12964___026___03195___2-242-28-36
    Average 94 stars, based on 1 article reviews
    cd38 inhibitor 1 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    Elabscience Biotechnology apc anti human cd38 antibody
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Apc Anti Human Cd38 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/APC+Anti-Human+CD38+Antibody/10__1007_slash_s00262___026___04553___2-119-53-57
    Average 93 stars, based on 1 article reviews
    apc anti human cd38 antibody - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    OriGene ggtccaagtgatgctcaatggg
    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or <t>78c</t> from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.
    Ggtccaagtgatgctcaatggg, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd38/Cd38+Mouse+qPCR+Primer+Pair/pm42244013-162-8-13
    Average 94 stars, based on 1 article reviews
    ggtccaagtgatgctcaatggg - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or 78c from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.

    Journal: Advanced Science

    Article Title: Bone Marrow Macrophage STING Signaling is a Direct Nexus Linking Marrow Adipose Expansion to NAD + Depletion and Osteogenic Decline in Skeletal Aging

    doi: 10.1002/advs.77571

    Figure Lengend Snippet: Enhanced CD38 ecto‐NADase activity in BMMs during skeletal aging impairs NMN availability to BMSCs. (a) qPCR analysis of Cd38 , Cdkn2a , Cdkn1a , Nos2 , Tnfα , IL1b , IL6 , Ym1 , Fizz1 , and Arg1 mRNA levels in bone marrow from 3‐ or 18‐month‐old WT and 18‐month‐old Sting ΔMφ mice ( n = 6 biological replicates). (b—d) Representative micro‐CT images (b) and quantitative analysis of BV/TV, Tb. N, Tb.Th, and Tb.Sp (c,d) from AAV9‐BMM‐shNC‐treated or AAV9‐BMM‐sh Cd38 ‐treated male mice ( n = 6 biological replicates). (e) WST‐8 analysis of NAD + levels in 3, 10, 18, and 24 M‐BMSCs ( n = 6 biological replicates). (f) WST‐8 analysis of NAD + levels in BMSCs under co‐culture conditions ( n = 6 biological replicates). (g) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice treated with PBS or 78c from 16‐ to 18‐month‐old ( n = 6 biological replicates). (h) WST‐8 analysis of NAD + levels in BMSCs from the bone marrow of mice injected with AAV9 for BMM‐specific overexpression of WT or Δ49 mouse Cd38 ( n = 6 biological replicates). Data are shown as mean ± SD.

    Article Snippet: In parallel, 16‐month‐old male C57BL/6J mice received 78c (MedChemExpress, HY‐123999; 10 mg/kg) or PBS intraperitoneally 5 days per week for 8 weeks.

    Techniques: Activity Assay, Analysis, Micro-CT, Co-culture Assay, Injection, Over Expression