Journal: International Journal of Molecular Sciences

Article Title: Smooth-Muscle-Cell-Specific Deletion of CD38 Protects Mice from AngII-Induced Abdominal Aortic Aneurysm through Inhibiting Vascular Remodeling

doi: 10.3390/ijms25084356

Figure Lengend Snippet: CD38 was upregulated in mice with AngII-induced abdominal aortic aneurysm (AAA) and the generation of smooth-muscle-cell (SMC)-specific deletion of CD38 and conventional deletion of Apoe double-knockout (CD38 SMA /Apoe −/− ) mice. ( A ) The images of CD38 expressions were obtained from immunofluorescence analysis of mice with AngII-induced AAA. ( B ) CD38 expression was quantitatively analyzed in ( A ). N = 6; ** p < 0.01. ( C ) Apoe alleles, WT, CD38 flx/flx , and Cre recombinase transgene were validated using PCR, respectively. ( D ) The deletion of CD38 in aortic smooth muscle cells was confirmed using Western blot analysis in CD38 flx/flx Cre SMA Apoe −/− mice. ( E ) Analysis of ( D ). N = 3; **** p < 0.0001. Note: CD38 flx/flx Apoe −/− and CD38 SMA Apoe −/− are referred to as “CD38 Fl/Fl “ and “CD38 SMA ”, respectively. Scale bar: 40 μm in ( A ).

Article Snippet: CD38 Fl/Fl mice were generated by Cyagen Biosciences Inc. (Suzhou, China).

Techniques: Double Knockout, Immunofluorescence, Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Smooth-Muscle-Cell-Specific Deletion of CD38 Protects Mice from AngII-Induced Abdominal Aortic Aneurysm through Inhibiting Vascular Remodeling

doi: 10.3390/ijms25084356

Figure Lengend Snippet: SMC-specific CD38 deficiency (CD38 SKO ) protected against AngII-induced AAA formation in mice. ( A ) Representative images of the entire aortas were obtained from CD38 Fl/Fl and CD38 SKO mice with or without AngII infusion. ( B ) The incidence of AAA was calculated from CD38 Fl/Fl and CD38 SKO mice. The difference in the incidence rate was analyzed with the χ 2 test; ** p < 0.01. ( C ) The maximal abdominal aortic diameters were quantitatively measured for each group; N = 6–10; *** p < 0.001, **** p < 0.0001. ( D ) The representative images were taken from the ultrasonography of four groups. ( E ) Blood pressures were measured from the four groups after 4-week AngII infusion. N = 6–10; * p < 0.05. Scale bar: 1 cm in ( A ).

Article Snippet: CD38 Fl/Fl mice were generated by Cyagen Biosciences Inc. (Suzhou, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Smooth-Muscle-Cell-Specific Deletion of CD38 Protects Mice from AngII-Induced Abdominal Aortic Aneurysm through Inhibiting Vascular Remodeling

doi: 10.3390/ijms25084356

Figure Lengend Snippet: CD38 deficiency in SMCs protected against AngII-induced vascular remodeling in mice. ( A ) Representative images of HE staining. ( B ) Quantification of aortic media thickness. ( C ) Quantification of aortic inner diameter corrected by perimeter. ( D ) Representative images of Masson staining. ( F ) Representative images of EVG staining. ( E ) Quantification of collagen content in ( D ). ( G ) Quantification of elastin degradation grade in ( F ). N = 6–10; * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001. Scale bar: 300 μm in 100× and 60 μm in 400× of ( A , D , F ).

Article Snippet: CD38 Fl/Fl mice were generated by Cyagen Biosciences Inc. (Suzhou, China).

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Smooth-Muscle-Cell-Specific Deletion of CD38 Protects Mice from AngII-Induced Abdominal Aortic Aneurysm through Inhibiting Vascular Remodeling

doi: 10.3390/ijms25084356

Figure Lengend Snippet: SMC-specific deletion of CD38 inhibited AngII-induced phenotype switch in VSMCs. ( A ) The representative images and quantitative expressions of α-SMA ( A , B ), SM22α ( C , D ), MYH11 ( E , F ), and Vimentin ( G , H ) were analyzed via immunofluorescent staining. N = 6; * p < 0.05, *** p < 0.001, **** p < 0.0001. Scale bar: 60 μm in ( A , C , E , G ).

Article Snippet: CD38 Fl/Fl mice were generated by Cyagen Biosciences Inc. (Suzhou, China).

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Smooth-Muscle-Cell-Specific Deletion of CD38 Protects Mice from AngII-Induced Abdominal Aortic Aneurysm through Inhibiting Vascular Remodeling

doi: 10.3390/ijms25084356

Figure Lengend Snippet: CD38 deficiency in SMCs reversed the AngII-induced decrease in the expression of SMC contractile markers. Primary VSMCs of CD38 Fl/Fl and CD38 SKO mice were starved for 48 h and pretreated with PD123319 (an AT2R inhibitor) for 2 h and Oss-128167 for 1h, and then AngII was added with or without Oss-128167 (a SIRT 6 inhibitor) for 24 h. ( A ) The protein expressions of α-SMA and SM22α were determined by Western blot under AngII stimulation. ( B ) The quantitative analysis in ( A ). ( C ) The protein expression of SM22α was determined by Western blot under AngII or Oss-128167 treatment. ( D ) The quantitative analysis in ( C ). N = 3; * p <0.05, ** p <0.01.

Article Snippet: CD38 Fl/Fl mice were generated by Cyagen Biosciences Inc. (Suzhou, China).

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Smooth-Muscle-Cell-Specific Deletion of CD38 Protects Mice from AngII-Induced Abdominal Aortic Aneurysm through Inhibiting Vascular Remodeling

doi: 10.3390/ijms25084356

Figure Lengend Snippet: CD38 deficiency in SMCs reduced the AngII-induced infiltration of inflammatory cells in the aortas of CD38 SMA mice. The representative images of CD68 ( A ), F4/80 ( B ), and VCAM-1 ( D ) were obtained via immunofluorescent staining in mouse aortas, and the representative images of MMP9 ( C ) were obtained via immunochemical analysis in mouse aortas. MMP9 is shown in brown color pointed by arrow. ( E ) The fluorescent intensities were quantitatively analyzed for VCAM-1. *** p < 0.001, * p < 0.05. Isolated primary VSMCs were used for ( F – J ). Cells were starved for 48 h and pretreated with PD123319 (an AT2R inhibitor) for 2 h and the Ex527 for 1h, then AngII was added with or without Ex527 (a SIRT 1 inhibitor) for 24 h. ( F ) The protein expression of VCAM-1 was determined by Western blot with AngII (1 μM) stimulation. ( G ) The protein expressions of VCAM-1, P65, and acetyl-P65 were determined by Western blot with AngII (1 μM) and Ex527 (10 μM) treatments. ( H – J ) The quantitative analysis of the expressions of VCAM-1 ( H ), Acetl-P65/P65 ( I ) with the treatment of Ex527, and VCAM-1 ( J ) with the treatment of Ex527. N = 3; * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: 60 μm in ( A , D ), 50 μm in ( B ), and 100 μm in ( C ).

Article Snippet: CD38 Fl/Fl mice were generated by Cyagen Biosciences Inc. (Suzhou, China).

Techniques: Staining, Isolation, Expressing, Western Blot

Journal: Cell metabolism

Article Title: A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline

doi: 10.1016/j.cmet.2018.03.016

Figure Lengend Snippet: (A) Hydrolase activity of recombinant human CD38 (rhCD38) in the presence of 78c, using 1,N6-ethenoadenine dinucleotide (ε-NAD+) as substrate (n=3 experiments, IC50 17.7 nM). Inset shows the structure of 78c. R1=H; R2=Me; R3=trans-4- OCH2CH2OMe-cyclohexyl.

Article Snippet: Recombinant human CD38 , R & D Systems , Cat#2404-AC-010.

Techniques: Activity Assay, Recombinant

Journal: Cell metabolism

Article Title: A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline

doi: 10.1016/j.cmet.2018.03.016

Figure Lengend Snippet: (A) Immunofluorescent localization of CD38 (red) expression in mouse liver. Sections were co-stained for the pan-leukocyte marker CD45 (green). Hoechst-stained nuclei are shown in blue. Arrow heads (white) indicate lack of CD38 in hepatocytes. Arrows (yellow) show sinusoidal distribution of CD38. Right image depicts a lobular area with a centrally located immune cells cluster. CV= central vein. Scale bar represents 50 μm.

Article Snippet: Recombinant human CD38 , R & D Systems , Cat#2404-AC-010.

Techniques: Expressing, Staining, Marker

Journal: Cell metabolism

Article Title: A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline

doi: 10.1016/j.cmet.2018.03.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Recombinant human CD38 , R & D Systems , Cat#2404-AC-010.

Techniques: Control, Recombinant, Transfection, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Activity Assay, Colorimetric Assay, Reverse Transcription, Luminescence Assay, Derivative Assay, Plasmid Preparation, Modification, Clone Assay, Mutagenesis, Construct, Software

Journal: Cell metabolism

Article Title: A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline

doi: 10.1016/j.cmet.2018.03.016

Figure Lengend Snippet: CD38 E230Q PCR Assays

Article Snippet: Recombinant human CD38 , R & D Systems , Cat#2404-AC-010.

Techniques:

Journal: Cell metabolism

Article Title: A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD + decline

doi: 10.1016/j.cmet.2018.03.016

Figure Lengend Snippet: TaqMan Gene Expression Assays

Article Snippet: Recombinant human CD38 , R & D Systems , Cat#2404-AC-010.

Techniques: Gene Expression

Journal: International Journal of Nanomedicine

Article Title: GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells

doi: 10.2147/IJN.S256104

Figure Lengend Snippet: Design of Dosages and Study Groups

Article Snippet: Standard SYBR Green PCR kit was taken from Fermentas [Germany], ALDEFLUORTM Kit and Methylcellulose from stem cell technologies [Canada] and CD34 and CD38 MicroBead Kit from Milteny Biotec [USA].

Techniques: Control

Journal: International Journal of Nanomedicine

Article Title: GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells

doi: 10.2147/IJN.S256104

Figure Lengend Snippet: ( A ) Dot plot diagram of the analysis of CD34 + CD38 − cells treated with GFc7 growth nanofactor in an FBS-free medium (Group C, Test) on days 1, 4, 7, 10 and 13. ( B ) Dot plot diagram of the analysis of CD34 + CD38 − cells treated without GFc7 growth nanofactor in an FBS-free medium (Group C, Control) on days 1, 4, 7, 10 and 13. ( C ) A comparison between the percentage of CD34 + CD38 − cells treated with and without GFc7 growth nanofactor (Group C, FBS-free medium). ( D ) A comparison between the absolute count of CD34 + CD38 − cells treated with and without GFc7 growth nanofactor (Group C, FBS-free medium).

Article Snippet: Standard SYBR Green PCR kit was taken from Fermentas [Germany], ALDEFLUORTM Kit and Methylcellulose from stem cell technologies [Canada] and CD34 and CD38 MicroBead Kit from Milteny Biotec [USA].

Techniques: Control, Comparison

Journal: International Journal of Nanomedicine

Article Title: GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells

doi: 10.2147/IJN.S256104

Figure Lengend Snippet: ( A ) Dot plot diagram of the analysis of CD34 + CD38 − cells treated with GFc7 growth nanofactor in an FBS medium (Group D, Test) on days 1, 4, 7, 10 and 13. ( B ) Dot plot diagram of the analysis of CD34 + CD38 − cells treated without GFc7 growth nanofactor in an FBS medium (Group D, Control) on days 1, 4, 7, 10 and 13. ( C ) A comparison between the percentage of CD34 + CD38 − cells treated with and without GFc7 growth nanofactor (Group D, FBS medium). ( D ) A comparison between the absolute count of CD34 + CD38 − cells treated with and without GFc7 growth nanofactor (Group D, FBS medium).

Article Snippet: Standard SYBR Green PCR kit was taken from Fermentas [Germany], ALDEFLUORTM Kit and Methylcellulose from stem cell technologies [Canada] and CD34 and CD38 MicroBead Kit from Milteny Biotec [USA].

Techniques: Control, Comparison

Journal: Cell

Article Title: Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in rhesus macaques coincides with anamnestic antibody response in the lung

doi: 10.1016/j.cell.2021.12.002

Figure Lengend Snippet:

Article Snippet: The following antibodies were used (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127, BD Biosciences), IgA Dylight 405 (goat polyclonal, Jackson Immunoresearch Inc), CD20 BV570 (clone 2H7, Biolegend), CD27 BV650 (clone O323, Biolegend), CD14 BV785 (clone M5E2, Biolegend), CD16 BUV496 (clone 3G8, BD Biosciences), IgG Alexa 700 (clone G18-145, BD Biosciences), CD3 APC-Cy7 (clone SP34-2, BD Biosciences), CD38 PE (clone OKT10, Caprico Biotechnologies), CD21 PE-Cy5 (clone B-ly4, BD Biosciences), and CXCR5 PE-Cy7 (clone MU5UBEE, Thermo Fisher Scientific).

Techniques: Labeling, Virus, Neutralization, Recombinant, Transfection, Staining, Multiplex Assay, Diagnostic Assay, Software

Journal: International journal of molecular sciences

Article Title: CRISPR/nCas9-Edited CD34+ Cells Rescue Mucopolysaccharidosis IVA Fibroblasts Phenotype.

doi: 10.3390/ijms26094334

Figure Lengend Snippet: Figure 2. Stemness evaluation of CRISPR/nCas9-edited CD34+ cells. (a) Percentage of positive cells for CD34, CD38, and CD45 (n = 6). (b) Differentiation potential was detected by the expression level of the differentiation markers CD15, CD14, and CD235a (n = 12). Total number of colonies formed per plate (left) and the number of colony types of progenitor cells and mature multilineage colonies (right). (c) Phase-contrast microscopy of representative BFU-E, CFU-G, and CFU-M in CD34+ cells transfected with or without CRISPR/nCas9. Scale bar, 50 µm (d) Proliferation index (n = 5). (e) Cell cycle distribution (n = 5). Mock: Unedited CD34+ cells. ns: no significant differences.

Article Snippet: Cells were stained with antihuman CD34+ APC antibody (1:50, Cat. 130-113-176, Miltenyi Biotec, Bergisch Gladbach, Germany), anti-human CD38+ PE antibody (1:50, Cat. 130-113-431, REAfinityTM, Miltenyi Biotec), and anti-human CD45+ FITC antibody (1:50, Cat. 130-110-631, Miltenyi Biotec).

Techniques: CRISPR, Expressing, Microscopy, Transfection

Journal: Cell Reports Methods

Article Title: Generation, expansion, gene delivery, and single-cell profiling in rhesus macaque plasma B cells

doi: 10.1016/j.crmeth.2024.100878

Figure Lengend Snippet:

Article Snippet: anti-CD38 FITC clone: OKT10 , Caprico Biotechnologies , 100815.

Techniques: Enzyme-linked Immunospot, Isolation, Enzyme-linked Immunosorbent Assay, Recombinant, Software