macs human cd34 microbead kit (Miltenyi Biotec)

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Miltenyi Biotec macs human cd34 microbead kit
Macs Human Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd34/CD34+MicroBead+Kit%2C+human+-+lyophilized/pm42478052-103-2-7
Average 97 stars, based on 152 article reviews

macs human cd34 microbead kit - by Bioz Stars, 2026-11

97/100 stars

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Purification:

Article Title: Multiplex base editing of BCL11A regulatory elements to treat sickle cell disease.
Article Snippet: .. HSPCs were purified by immunomagnetic selection with a manual magnetic cell separator (Miltenyi Biotec) after immunostaining with the CD34 MicroBead Kit (Miltenyi Biotec). .. Forty-eight hours before transfection, CD34 + cells were thawed and cultured at a concentration of 5x10 5 cells/ml in the ‘‘HSPC medium’’ containing StemSpan (STEMCELL Technologies) supplemented with penicillin/streptomycin (Gibco), 250 nM StemRegenin1 (STEMCELL Technologies), and the following recombinant human cytokines (PeproTech): human stem cell factor (SCF) (300 ng/mL), FMS-like tyrosine kinase 3 ligand (Flt-3L) (300 ng/mL), thrombopoietin (TPO) (100 ng/mL), and interleukin-3 (IL-3) (60 ng/mL).

Selection:

Article Title: Quantifying HLA transcripts by genotype in chimeric mixtures at single-cell resolution
Article Snippet: When viability < 80%, live cells were enriched using magnetic removal of dead cells (Miltenyi Biotec 130-090-101). .. When applicable, at most 10 million cells were enriched for cells with CD34 cell surface markers using either Miltenyi magnetic CD34 Microbead kit (Miltenyi Biotec #130-097-047) or EasySep human CD34 Positive Selection kit (Stemcell #17856) with an expected yield of ∼500,000 CD34-enriched cells. .. For patient ‘AML15’, instead of a CD34+ enrichment, cells with CD117 markers were enriched using Miltenyi magnetic CD117 Microbead kit (Miltenyi Biotec #130-091-332) in a decision based on the patient’s leukemia characteristics.

Immunostaining:

Isolation:

Article Title: Optimised RAG1 deficient gene therapy
Article Snippet: Human bone marrow from children diagnosed with SCID was obtained according to the Medical Ethical Committee and IRB guidelines at Leiden University Medical Center The patient was a compound heterozygote was the following confirmed mutations: RAG1 allele 1 C 256-257 deletion AA, allele 2 C 1677 G>T Mononuclear cells were separated by Ficoll gradient centrifugation, frozen in fetal calf serum (Grenier Bio-one)/10% DMSO (Sigma-Aldrich) and stored in liquid nitrogen. .. After thawing, human CD34+ cells were isolated using CD34 MicroBead UltraPure Kit (Milteny Biotec). .. Enriched CD34+ cells were stimulated overnight in X-VIV015 without Gentamycin and phenolred (Lonza)—1% human albumin (200 g/L; Sanquin)—Pen/Strep medium supplemented with 300 ng/ml huSCF (Milteny Biotec), 100 ng/ml huTPO (Milteny Biotec), 300 ng/ml huFlt3L (Milteny Biotec) and 10 ng/ml huIL3 (Milteny Biotec).

Article Title: Apatinib enhances targeted immunotherapy via the IL-6-gp130-PI3K pathway in HER2-positive gastric cancer.
Article Snippet: Fluorescence intensity was analyzed in ImageJ software as a semi-quantitative measure of protein expression. .. Subsequently, a MACS human CD34 MicroBead Kit (Miltenyi Biotec, GoldBach, Germany) was used to select hCD34+ cells from the isolated mononuclear cells, in accordance with the manufacturer’s instructions. .. In accordance with previously reported protocols for isolation of human CD34+ (hCD34+) cells29, mononuclear cells were isolated from cord blood in a 1:1 ratio with lymphocyte separation solution (Miltenyi Biotec, GoldBach, Germany), then centrifuged at 2,000 rpm for 20 min.

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macs human cd34 microbead kit (Miltenyi Biotec)

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