Journal: Cellular & molecular biology letters

Article Title: Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler.

doi: 10.1186/s11658-024-00573-x

Figure Lengend Snippet: Fig. 4 Characteristics of tissue layers of the antler growth center and isolated RM cells. A Immunofluorescence assay of CD73, CD90, Nestin, CD34, and Prrx1 (red color) in the RM, PC, and CA layers, respectively. B Third passage of cultured cells from the RM layer were identified using mesenchymal stem cell markers: CD73 and CD90 (red), Nestin and CD34 (green), respectively; nuclei were stained blue with DAPI. C Chondrogenic differentiation of RM cells, Alcian blue, and Col II immunofluorescence staining. D Osteogenic differentiation of RM cells with Alizarin red staining. E Adipogenic differentiation of RM cells with Oil Red O staining (scale bars, 125 μm and magnification ×200)

Article Snippet: The cells and tissues were fixed, permeabilized, blocked, and incubated overnight with primary antibodies: CD90 (1:300, proteintech, USA, 66766-1-lg), CD73 (1:1000, proteintech, USA, 12231- 1-AP), Nestin (1:300, BIOSS, China, bs-0008R), CD34 (1:300, proteintech, USA, 14486- 1-AP), and Prrx1 (1:200, Absin, China, abs134576).

Techniques: Isolation, Immunofluorescence, Cell Culture, Staining

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Purity assessment of the CD34+ cell fraction by flow cytometry . The initial purity of CD34+ cells after separation through single column was 47.5%. The CD34- fraction was 99.4% pure. CD34+ and CD34- cell populations were defined by first gating on forward and side scatter properties excluding platelets and debris. Subsequent gates were set to exclude >99% of control cells labeled with isotype-specific antibody. Percentages indicating the purity of isolated cell fractions are shown for both plots. Abbreviations: SSC, side scatter; PE, phycoerythrin.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Flow Cytometry, Control, Labeling, Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Purity assessment of CD34+ cell fraction after one or two column separations . A) The CD34+ cell fraction was 78% pure after the first column separation. B) A 92% pure CD34+ cell faction was obtained by an additional labeling step in connection with a second column separation. CD34+ cell populations were defined by first gating on forward and side scatter properties excluding platelets and debris. Subsequent gates were set to exclude >99% of control cells labeled with isotype-specific antibody. Percentages indicating the purity of isolated cell fractions are shown for both plots. Abbreviations: SSC, side scatter; PE, phycoerythrin.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Labeling, Control, Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Purity assessment of CD34+/-, CD133+/- and Lin-/+ cell fractions . A) Purities for CD34+ and CD34- cell factions were 97.1% and 99.1%, respectively. B) Purities for CD133+ and CD133- fractions were 93.6% and 99.1%, respectively. C) Purities for Lin- and Lin+ cell factions were 97.0% and 99.5%, respectively. CD34+/-, CD133+/- cell populations were defined by first gating on forward and side scatter properties excluding platelets and debris. Subsequent gates were set to exclude >99% of control cells labeled with isotype-specific antibody. Percentages indicating the purity of isolated cell fractions are shown for both plots. Abbreviations: SSC, side scatter; IgG, immunoglobulin; PE, phycoerythrin.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Control, Labeling, Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: A chart of the optimized protocols to isolate CD34+/-, CD133+/- and Lin-/+ cells from cord blood . Isolation of CD34/- and CD133+/- cells was performed using Direct CD34 Progenitor Cell Isolation Kit (#130-046-702, Miltenyi Biotec) and CD133 Cell Isolation Kit (#130-050-801, Miltenyi Biotec), respectively. Lin-/+ cells were isolated using StemSep Human Progenitor Enrichment Kit (#14056, StemCell Technologies). For all magnetic separations, MACS columns and separators (Miltenyi Biotech) were used. Abbreviations: MNC, mononuclear cells; Buffer, PBS pH 7.2 supplemented with 0.5% bovine serum albumin and 2 mM EDTA or 0.6% ACD/A.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Isolation, Cell Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Frequency of different types of CFU colonies within CD34+, CD133+, Lin- and MNC populations.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: