Staining:Article Title: CRISPR/Cas9-mediated t(4;11) translocation in human hematopoietic stem/precursor cells demonstrates plasticity to differentiate into either the myeloid or lymphoid lineage
Article Snippet: The samples were measured with the BD FACSVerseTM instrument and analyzed with the FlowJo software. .. The following antibodies have been used: anti-human CD45-FITC (clone REA747; Miltenyi; #130-110-769), anti-human CD33-PE-Vio770 (clone REA775; Miltenyi; #130-111-138), anti-human CD123-APC-Vio770 (clone AC145; Miltenyi; #130-113-885), anti-human CD56-APC (clone REA196; Miltenyi; #130-113-872), anti-human CD19-BV510 (clone HIB19; BD Biosciences; #740164), anti-human CD20-V450 (clone L27; BD Biosciences; #561164), anti-human lineage cocktail 1(lin 1) [anti-human CD3-FITC (clone SK7), anti-human CD14-FITC (clone 3G8), anti-human CD16-FITC (clone SJ25C1), anti-human CD19-FITC (clone L27), anti-human CD20-FITC (clone MφP9), anti-human CD56-FITC (clone NCAM16.2)] (BD Biosciences; #340546), anti-human CD10-APC-Vio770 (clone 97C5; Miltenyi; #130-128-300), anti-human CD90-APC-Vio770 (clone REA879; Miltenyi; #130-114-905), anti-human CD45RA-BV-510 (clone HI100; BD Biosciences; #563031) and the viability stain 7-AAD (Miltenyi; #130-111-568). .. The CellTraceTM Violet Cell Proliferation Kit (Thermo Scientific; # C34557 ) was used to track cell proliferation and cell division.
Article Title: CRISPR/Cas9-mediated t(4;11) translocation in human hematopoietic stem/precursor cells demonstrates plasticity to differentiate into either the myeloid or lymphoid lineage.
Article Snippet: The samples were measured with the BD FACSVerseTM instrument and analyzed with the FlowJo software. .. The following antibodies have been used: antihuman CD45-FITC (clone REA747; Miltenyi; #130-110-769), anti-human CD33-PE-Vio770 (clone REA775; Miltenyi; #130-111-138), anti-human CD123-APC-Vio770 (clone AC145; Miltenyi; #130-113-885), anti-human CD56-APC (clone REA196; Miltenyi; #130-113-872), anti-human CD19BV510 (clone HIB19; BD Biosciences; #740164), anti-human CD20-V450 (clone L27; BD Biosciences; #561164), anti-human lineage cocktail 1(lin 1) [anti-human CD3-FITC (clone SK7), anti-human CD14-FITC (clone 3G8), antihuman CD16-FITC (clone SJ25C1), anti-human CD19-FITC (clone L27), antihuman CD20-FITC (clone MφP9), anti-human CD56-FITC (clone NCAM16.2)] (BD Biosciences; #340546), anti-human CD10-APC-Vio770 (clone 97C5; Miltenyi; #130-128-300), anti-human CD90-APC-Vio770 (clone REA879; Miltenyi; #130-114-905), anti-human CD45RA-BV-510 (clone HI100; BD Biosciences; #563031) and the viability stain 7-AAD (Miltenyi; #130-111- 568). .. Cell trace proliferation assay The CellTraceTM Violet Cell Proliferation Kit (Thermo Scientific; #C34557) was used to track cell proliferation and cell division.
Recombinant:Article Title: A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue.
Article Snippet: .. Scientific Reports | (2025) 15:15175 7| https://doi.org/10.1038/s41598-025-99877-x Reagent or resource Source Identifier Antibodies FITC anti-human CD33 (clone REA775) Miltenyi Biotec Cat #130-111-027 PE anti-human CD11b (clone REA713) Miltenyi Biotec Cat #130-110-553 PE-Cy7 anti-human HLA-DR (clone REA805) Miltenyi Biotec Cat #130-111-791 Biological samples Human non-small cell lung cancer tumor biopsies Hospital La Ribera, Alzira See Table S1 Human non-small cell lung cancer peripheral blood Hospital La Ribera, Alzira See Table S1 Chemicals, peptides, and recombinant proteins DMEM/F-12 medium Gibco Cat #31,331-028 Antibiotic–antimycotic Biowest Cat #L0010 Phosphate-buffered saline (PBS) Biowest Cat #X0515 RosetteSep DM-M density medium STEMCELL Cat #15725 Carboxyfluorescein diacetate succinimidyl ester (CFSE) Life technologies Cat # C34554 RPMI1640 medium Biowest Cat #L0498 Fetal bovine serum (FBS) Gibco Cat #A3160502 Critical commercial assays Tumor dissociation kit Miltenyi Biotec Cat #130-095-929 Anti-HLA-DR Ab-coated magnetic beads Miltenyi Biotec Cat #130-046-101 Anti-CD14 Ab-coated magnetic beads Miltenyi Biotec Cat #130-050-201 RosetteSep HLA myeloid cell enrichment cocktail STEMCELL Cat #15272HLA E.Z.N.A. total RNA kit Omega Bio-tek Cat #R6934-02 PrimeScript RT reagent kit Takara Cat #RR037A SYBR Premix Ex TaqTM DNA Polymerase Takara Cat #RR420W Anti-CD8 Ab-coated magnetic beads Miltenyi Biotec Cat #130-045-201 T Cell TransAct Miltenyi Biotec Cat #130-111-160 Oligonucleotides Primer: ACTB, forward: A C A G A G C C T C G C C T T T G C Condalab N/A Primer: ARG1, forward: C C G T G C C C A G A T T C T G A C T T Condalab N/A Primer: IDO1, forward: A T G A A G A A G T G G G C T T T G C T C T Condalab N/A Primer: PD-L1, forward: C C T A C T G G C A T T T G C T G A A C G Condalab N/A Primer: NOS2, forward: C T G G A T T T G G C T G G T C C C T Condalab N/A Primer: COX2, forward: C T T G C A G T G A G C G T C A G G A G Condalab N/A Primer: VEGFA, forward: T G A G C T T C C T A C A G C A C A A C A A Condalab N/A Primer: S100A8, forward: A T G C C G T C T A C A G G G A T G A C Condalab N/A Primer: S100A9, forward: C A A T A C T C T G T G A A G C T G G G G Condalab N/A Primer: LOX1, forward: A G G T C T T C A G T T T C T T T A C T C T C C Condalab N/A Software and algorithms BD FACSDiva version 8.0.1 BD Biosciences RRID:SCR_001456 Kaluza analysis version 2.1 Beckman Coulter RRID:SCR_016182 Prism version 9.2.0 GraphPad Software RRID:SCR_002798 ..
Saline:Article Title: A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue.
Article Snippet: .. Scientific Reports | (2025) 15:15175 7| https://doi.org/10.1038/s41598-025-99877-x Reagent or resource Source Identifier Antibodies FITC anti-human CD33 (clone REA775) Miltenyi Biotec Cat #130-111-027 PE anti-human CD11b (clone REA713) Miltenyi Biotec Cat #130-110-553 PE-Cy7 anti-human HLA-DR (clone REA805) Miltenyi Biotec Cat #130-111-791 Biological samples Human non-small cell lung cancer tumor biopsies Hospital La Ribera, Alzira See Table S1 Human non-small cell lung cancer peripheral blood Hospital La Ribera, Alzira See Table S1 Chemicals, peptides, and recombinant proteins DMEM/F-12 medium Gibco Cat #31,331-028 Antibiotic–antimycotic Biowest Cat #L0010 Phosphate-buffered saline (PBS) Biowest Cat #X0515 RosetteSep DM-M density medium STEMCELL Cat #15725 Carboxyfluorescein diacetate succinimidyl ester (CFSE) Life technologies Cat # C34554 RPMI1640 medium Biowest Cat #L0498 Fetal bovine serum (FBS) Gibco Cat #A3160502 Critical commercial assays Tumor dissociation kit Miltenyi Biotec Cat #130-095-929 Anti-HLA-DR Ab-coated magnetic beads Miltenyi Biotec Cat #130-046-101 Anti-CD14 Ab-coated magnetic beads Miltenyi Biotec Cat #130-050-201 RosetteSep HLA myeloid cell enrichment cocktail STEMCELL Cat #15272HLA E.Z.N.A. total RNA kit Omega Bio-tek Cat #R6934-02 PrimeScript RT reagent kit Takara Cat #RR037A SYBR Premix Ex TaqTM DNA Polymerase Takara Cat #RR420W Anti-CD8 Ab-coated magnetic beads Miltenyi Biotec Cat #130-045-201 T Cell TransAct Miltenyi Biotec Cat #130-111-160 Oligonucleotides Primer: ACTB, forward: A C A G A G C C T C G C C T T T G C Condalab N/A Primer: ARG1, forward: C C G T G C C C A G A T T C T G A C T T Condalab N/A Primer: IDO1, forward: A T G A A G A A G T G G G C T T T G C T C T Condalab N/A Primer: PD-L1, forward: C C T A C T G G C A T T T G C T G A A C G Condalab N/A Primer: NOS2, forward: C T G G A T T T G G C T G G T C C C T Condalab N/A Primer: COX2, forward: C T T G C A G T G A G C G T C A G G A G Condalab N/A Primer: VEGFA, forward: T G A G C T T C C T A C A G C A C A A C A A Condalab N/A Primer: S100A8, forward: A T G C C G T C T A C A G G G A T G A C Condalab N/A Primer: S100A9, forward: C A A T A C T C T G T G A A G C T G G G G Condalab N/A Primer: LOX1, forward: A G G T C T T C A G T T T C T T T A C T C T C C Condalab N/A Software and algorithms BD FACSDiva version 8.0.1 BD Biosciences RRID:SCR_001456 Kaluza analysis version 2.1 Beckman Coulter RRID:SCR_016182 Prism version 9.2.0 GraphPad Software RRID:SCR_002798 ..
Magnetic Beads:Article Title: A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue.
Article Snippet: .. Scientific Reports | (2025) 15:15175 7| https://doi.org/10.1038/s41598-025-99877-x Reagent or resource Source Identifier Antibodies FITC anti-human CD33 (clone REA775) Miltenyi Biotec Cat #130-111-027 PE anti-human CD11b (clone REA713) Miltenyi Biotec Cat #130-110-553 PE-Cy7 anti-human HLA-DR (clone REA805) Miltenyi Biotec Cat #130-111-791 Biological samples Human non-small cell lung cancer tumor biopsies Hospital La Ribera, Alzira See Table S1 Human non-small cell lung cancer peripheral blood Hospital La Ribera, Alzira See Table S1 Chemicals, peptides, and recombinant proteins DMEM/F-12 medium Gibco Cat #31,331-028 Antibiotic–antimycotic Biowest Cat #L0010 Phosphate-buffered saline (PBS) Biowest Cat #X0515 RosetteSep DM-M density medium STEMCELL Cat #15725 Carboxyfluorescein diacetate succinimidyl ester (CFSE) Life technologies Cat # C34554 RPMI1640 medium Biowest Cat #L0498 Fetal bovine serum (FBS) Gibco Cat #A3160502 Critical commercial assays Tumor dissociation kit Miltenyi Biotec Cat #130-095-929 Anti-HLA-DR Ab-coated magnetic beads Miltenyi Biotec Cat #130-046-101 Anti-CD14 Ab-coated magnetic beads Miltenyi Biotec Cat #130-050-201 RosetteSep HLA myeloid cell enrichment cocktail STEMCELL Cat #15272HLA E.Z.N.A. total RNA kit Omega Bio-tek Cat #R6934-02 PrimeScript RT reagent kit Takara Cat #RR037A SYBR Premix Ex TaqTM DNA Polymerase Takara Cat #RR420W Anti-CD8 Ab-coated magnetic beads Miltenyi Biotec Cat #130-045-201 T Cell TransAct Miltenyi Biotec Cat #130-111-160 Oligonucleotides Primer: ACTB, forward: A C A G A G C C T C G C C T T T G C Condalab N/A Primer: ARG1, forward: C C G T G C C C A G A T T C T G A C T T Condalab N/A Primer: IDO1, forward: A T G A A G A A G T G G G C T T T G C T C T Condalab N/A Primer: PD-L1, forward: C C T A C T G G C A T T T G C T G A A C G Condalab N/A Primer: NOS2, forward: C T G G A T T T G G C T G G T C C C T Condalab N/A Primer: COX2, forward: C T T G C A G T G A G C G T C A G G A G Condalab N/A Primer: VEGFA, forward: T G A G C T T C C T A C A G C A C A A C A A Condalab N/A Primer: S100A8, forward: A T G C C G T C T A C A G G G A T G A C Condalab N/A Primer: S100A9, forward: C A A T A C T C T G T G A A G C T G G G G Condalab N/A Primer: LOX1, forward: A G G T C T T C A G T T T C T T T A C T C T C C Condalab N/A Software and algorithms BD FACSDiva version 8.0.1 BD Biosciences RRID:SCR_001456 Kaluza analysis version 2.1 Beckman Coulter RRID:SCR_016182 Prism version 9.2.0 GraphPad Software RRID:SCR_002798 ..
Software:Article Title: A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue.
Article Snippet: .. Scientific Reports | (2025) 15:15175 7| https://doi.org/10.1038/s41598-025-99877-x Reagent or resource Source Identifier Antibodies FITC anti-human CD33 (clone REA775) Miltenyi Biotec Cat #130-111-027 PE anti-human CD11b (clone REA713) Miltenyi Biotec Cat #130-110-553 PE-Cy7 anti-human HLA-DR (clone REA805) Miltenyi Biotec Cat #130-111-791 Biological samples Human non-small cell lung cancer tumor biopsies Hospital La Ribera, Alzira See Table S1 Human non-small cell lung cancer peripheral blood Hospital La Ribera, Alzira See Table S1 Chemicals, peptides, and recombinant proteins DMEM/F-12 medium Gibco Cat #31,331-028 Antibiotic–antimycotic Biowest Cat #L0010 Phosphate-buffered saline (PBS) Biowest Cat #X0515 RosetteSep DM-M density medium STEMCELL Cat #15725 Carboxyfluorescein diacetate succinimidyl ester (CFSE) Life technologies Cat # C34554 RPMI1640 medium Biowest Cat #L0498 Fetal bovine serum (FBS) Gibco Cat #A3160502 Critical commercial assays Tumor dissociation kit Miltenyi Biotec Cat #130-095-929 Anti-HLA-DR Ab-coated magnetic beads Miltenyi Biotec Cat #130-046-101 Anti-CD14 Ab-coated magnetic beads Miltenyi Biotec Cat #130-050-201 RosetteSep HLA myeloid cell enrichment cocktail STEMCELL Cat #15272HLA E.Z.N.A. total RNA kit Omega Bio-tek Cat #R6934-02 PrimeScript RT reagent kit Takara Cat #RR037A SYBR Premix Ex TaqTM DNA Polymerase Takara Cat #RR420W Anti-CD8 Ab-coated magnetic beads Miltenyi Biotec Cat #130-045-201 T Cell TransAct Miltenyi Biotec Cat #130-111-160 Oligonucleotides Primer: ACTB, forward: A C A G A G C C T C G C C T T T G C Condalab N/A Primer: ARG1, forward: C C G T G C C C A G A T T C T G A C T T Condalab N/A Primer: IDO1, forward: A T G A A G A A G T G G G C T T T G C T C T Condalab N/A Primer: PD-L1, forward: C C T A C T G G C A T T T G C T G A A C G Condalab N/A Primer: NOS2, forward: C T G G A T T T G G C T G G T C C C T Condalab N/A Primer: COX2, forward: C T T G C A G T G A G C G T C A G G A G Condalab N/A Primer: VEGFA, forward: T G A G C T T C C T A C A G C A C A A C A A Condalab N/A Primer: S100A8, forward: A T G C C G T C T A C A G G G A T G A C Condalab N/A Primer: S100A9, forward: C A A T A C T C T G T G A A G C T G G G G Condalab N/A Primer: LOX1, forward: A G G T C T T C A G T T T C T T T A C T C T C C Condalab N/A Software and algorithms BD FACSDiva version 8.0.1 BD Biosciences RRID:SCR_001456 Kaluza analysis version 2.1 Beckman Coulter RRID:SCR_016182 Prism version 9.2.0 GraphPad Software RRID:SCR_002798 ..
other:Article Title: STING activation improves T-cell-engaging immunotherapy for acute myeloid leukemia
Article Snippet: CD33-APC REA775 WM53 Miltenyi Biotec BioLegend 130-111-019 303408 CD45-PE HI30 BioLegend 304008 CD107a-FITC H4A3 BioLegend 328606 CD253 (TRAIL)-PE/Cy7 RIK-2 BioLegend 308215 CD119 (IFNGR1)-PE GIR-208 BioLegend 308606 Granzyme B-PacBlue GB11 BioLegend 515408 ICAM1 (CD54)-APC-Fire750 HA58 BioLegend 353122 CD86-APC IT2.2 Bio Legend 305412 Caspase-3-FITC C92-605 BD Biosciences 559341 Phospho-IκBα (Ser32) rabbit mAb 14D4 Cell Signaling Technology 2859S cGAS rabbit mAb D1D3G Cell Signaling Technology 15102S Viperin MaP.VIP Merck Chemicals #MABF106 OAS1 rabbit mAb D1W3A Cell Signaling Technology 14498 Phospho-Stat2 (Tyr690) rabbit mAb D3P2P Cell Signaling Technology 88410
Activation Assay:Article Title: Early cytokine and chemokine signals shape the anti-AML activity of bispecific engager-secreting T cells
Article Snippet: 41BB.ζ 2022 Janssen Biotech CD3/CD28stimulating microbeads and IL-2 lentivirus Negative selection of pan T cells 27,28 afamitresgene autoleucel afami-cel, TECELRA αMAGEA4 TCR 2024 Adaptimmune CD3/CD28stimulating microbeads lentivirus No 29,30 4 Table S2. .. Antibody list Antibody Fluorophore Reference T-cell activation CD3 Miltenyi: 130-093-387 CD28 BD: 555725 ELISA Goat Anti-Mouse IgG, F(ab’)2 fragment specific Jackson Immunoresearch: 115-006-072 Bovine anti-goat HRP Jackson Immunoresearch 805-035-180 Purity panel – healthy donor day 0 and all patient-derived cells CD3 PerCP-Vio700 Miltenyi: 130-113-132 CD4 APC-Vio770 Miltenyi: 130-113-251 CD8 PE Miltenyi: 130-113-158 CD33 VioBlue Miltenyi: 130-111-141 CD123 BB515 BD: 567715 CD20 BV786 BD: 743611 Purity panel – healthy donor day 7 CD4 PerCP-Cy5.5 BD: 566923 CD8 FITC BD: 347313 CD33 BV421 BD: 744761 CD123 BV786 BD: 564196 CD20 APC BD: 559776 Immunophenotype panel CD4 BV750 BD: 747176 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA PE BD: 555489 LIVE/DEAD ThermoFisher: L34969 Immunophenotype panel with transduction marker CD4 PerCP-Cy5.5 BD: 560650 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA BV510 BD: 563031 CD20 PE BD: 555623 Exhaustion panel PD-1 Alexa 647 BD: 560838 LAG-3 PE BD: 565616 TIM-3 BV421 BD: 565562 CD4 BV750 BD: 747176 CD8 APC-Cy7 BD: 557760 LIVE/DEAD ThermoFisher: L34969 Murine peripheral blood panel hCD45 FITC BD: 555482 hCD20 APC BD: 559776 hCD33 BV421 BD: 744761 5 Table S3. ..
Enzyme-linked Immunosorbent Assay:Article Title: Early cytokine and chemokine signals shape the anti-AML activity of bispecific engager-secreting T cells
Article Snippet: 41BB.ζ 2022 Janssen Biotech CD3/CD28stimulating microbeads and IL-2 lentivirus Negative selection of pan T cells 27,28 afamitresgene autoleucel afami-cel, TECELRA αMAGEA4 TCR 2024 Adaptimmune CD3/CD28stimulating microbeads lentivirus No 29,30 4 Table S2. .. Antibody list Antibody Fluorophore Reference T-cell activation CD3 Miltenyi: 130-093-387 CD28 BD: 555725 ELISA Goat Anti-Mouse IgG, F(ab’)2 fragment specific Jackson Immunoresearch: 115-006-072 Bovine anti-goat HRP Jackson Immunoresearch 805-035-180 Purity panel – healthy donor day 0 and all patient-derived cells CD3 PerCP-Vio700 Miltenyi: 130-113-132 CD4 APC-Vio770 Miltenyi: 130-113-251 CD8 PE Miltenyi: 130-113-158 CD33 VioBlue Miltenyi: 130-111-141 CD123 BB515 BD: 567715 CD20 BV786 BD: 743611 Purity panel – healthy donor day 7 CD4 PerCP-Cy5.5 BD: 566923 CD8 FITC BD: 347313 CD33 BV421 BD: 744761 CD123 BV786 BD: 564196 CD20 APC BD: 559776 Immunophenotype panel CD4 BV750 BD: 747176 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA PE BD: 555489 LIVE/DEAD ThermoFisher: L34969 Immunophenotype panel with transduction marker CD4 PerCP-Cy5.5 BD: 560650 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA BV510 BD: 563031 CD20 PE BD: 555623 Exhaustion panel PD-1 Alexa 647 BD: 560838 LAG-3 PE BD: 565616 TIM-3 BV421 BD: 565562 CD4 BV750 BD: 747176 CD8 APC-Cy7 BD: 557760 LIVE/DEAD ThermoFisher: L34969 Murine peripheral blood panel hCD45 FITC BD: 555482 hCD20 APC BD: 559776 hCD33 BV421 BD: 744761 5 Table S3. ..
Transduction:Article Title: Early cytokine and chemokine signals shape the anti-AML activity of bispecific engager-secreting T cells
Article Snippet: 41BB.ζ 2022 Janssen Biotech CD3/CD28stimulating microbeads and IL-2 lentivirus Negative selection of pan T cells 27,28 afamitresgene autoleucel afami-cel, TECELRA αMAGEA4 TCR 2024 Adaptimmune CD3/CD28stimulating microbeads lentivirus No 29,30 4 Table S2. .. Antibody list Antibody Fluorophore Reference T-cell activation CD3 Miltenyi: 130-093-387 CD28 BD: 555725 ELISA Goat Anti-Mouse IgG, F(ab’)2 fragment specific Jackson Immunoresearch: 115-006-072 Bovine anti-goat HRP Jackson Immunoresearch 805-035-180 Purity panel – healthy donor day 0 and all patient-derived cells CD3 PerCP-Vio700 Miltenyi: 130-113-132 CD4 APC-Vio770 Miltenyi: 130-113-251 CD8 PE Miltenyi: 130-113-158 CD33 VioBlue Miltenyi: 130-111-141 CD123 BB515 BD: 567715 CD20 BV786 BD: 743611 Purity panel – healthy donor day 7 CD4 PerCP-Cy5.5 BD: 566923 CD8 FITC BD: 347313 CD33 BV421 BD: 744761 CD123 BV786 BD: 564196 CD20 APC BD: 559776 Immunophenotype panel CD4 BV750 BD: 747176 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA PE BD: 555489 LIVE/DEAD ThermoFisher: L34969 Immunophenotype panel with transduction marker CD4 PerCP-Cy5.5 BD: 560650 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA BV510 BD: 563031 CD20 PE BD: 555623 Exhaustion panel PD-1 Alexa 647 BD: 560838 LAG-3 PE BD: 565616 TIM-3 BV421 BD: 565562 CD4 BV750 BD: 747176 CD8 APC-Cy7 BD: 557760 LIVE/DEAD ThermoFisher: L34969 Murine peripheral blood panel hCD45 FITC BD: 555482 hCD20 APC BD: 559776 hCD33 BV421 BD: 744761 5 Table S3. ..
Marker:Article Title: Early cytokine and chemokine signals shape the anti-AML activity of bispecific engager-secreting T cells
Article Snippet: 41BB.ζ 2022 Janssen Biotech CD3/CD28stimulating microbeads and IL-2 lentivirus Negative selection of pan T cells 27,28 afamitresgene autoleucel afami-cel, TECELRA αMAGEA4 TCR 2024 Adaptimmune CD3/CD28stimulating microbeads lentivirus No 29,30 4 Table S2. .. Antibody list Antibody Fluorophore Reference T-cell activation CD3 Miltenyi: 130-093-387 CD28 BD: 555725 ELISA Goat Anti-Mouse IgG, F(ab’)2 fragment specific Jackson Immunoresearch: 115-006-072 Bovine anti-goat HRP Jackson Immunoresearch 805-035-180 Purity panel – healthy donor day 0 and all patient-derived cells CD3 PerCP-Vio700 Miltenyi: 130-113-132 CD4 APC-Vio770 Miltenyi: 130-113-251 CD8 PE Miltenyi: 130-113-158 CD33 VioBlue Miltenyi: 130-111-141 CD123 BB515 BD: 567715 CD20 BV786 BD: 743611 Purity panel – healthy donor day 7 CD4 PerCP-Cy5.5 BD: 566923 CD8 FITC BD: 347313 CD33 BV421 BD: 744761 CD123 BV786 BD: 564196 CD20 APC BD: 559776 Immunophenotype panel CD4 BV750 BD: 747176 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA PE BD: 555489 LIVE/DEAD ThermoFisher: L34969 Immunophenotype panel with transduction marker CD4 PerCP-Cy5.5 BD: 560650 CD8 BV421 BD: 562428 CCR7 APC BD: 566762 CD45RA BV510 BD: 563031 CD20 PE BD: 555623 Exhaustion panel PD-1 Alexa 647 BD: 560838 LAG-3 PE BD: 565616 TIM-3 BV421 BD: 565562 CD4 BV750 BD: 747176 CD8 APC-Cy7 BD: 557760 LIVE/DEAD ThermoFisher: L34969 Murine peripheral blood panel hCD45 FITC BD: 555482 hCD20 APC BD: 559776 hCD33 BV421 BD: 744761 5 Table S3. ..
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