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anti mouse  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti mouse
    Anti Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31/CD31+Antibody%2C+anti-mouse/pmc13101284-4-2-8
    Average 95 stars, based on 69 article reviews
    anti mouse - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Single Cell:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: Myelin was removed by resuspending cell pellets in 0.9 M sucrose in DPBS with calcium and magnesium (Gibco; 14040) and centrifuging (850 xg, 15 minutes, 4 °C). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/Fire (BioLegend; 105348), 1:50 ACSA2–PE-Vio 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluor506 (Invitrogen eBioscience; 65-0866-14).

    Suspension:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: Myelin was removed by resuspending cell pellets in 0.9 M sucrose in DPBS with calcium and magnesium (Gibco; 14040) and centrifuging (850 xg, 15 minutes, 4 °C). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/Fire (BioLegend; 105348), 1:50 ACSA2–PE-Vio 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluor506 (Invitrogen eBioscience; 65-0866-14).

    Blocking Assay:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: Myelin was removed by resuspending cell pellets in 0.9 M sucrose in DPBS with calcium and magnesium (Gibco; 14040) and centrifuging (850 xg, 15 minutes, 4 °C). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/Fire (BioLegend; 105348), 1:50 ACSA2–PE-Vio 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluor506 (Invitrogen eBioscience; 65-0866-14).

    Staining:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: Myelin was removed by resuspending cell pellets in 0.9 M sucrose in DPBS with calcium and magnesium (Gibco; 14040) and centrifuging (850 xg, 15 minutes, 4 °C). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/Fire (BioLegend; 105348), 1:50 ACSA2–PE-Vio 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluor506 (Invitrogen eBioscience; 65-0866-14).

    Article Title: Fibroadipogenic progenitor-secreted prostaglandin E2 coordinates stem cell fate via autocrine and paracrine crosstalk in healthy and dystrophic muscle.
    Article Snippet: Muscle cells were dissociated by using the gentle MACS dissociator (Miltenyi Biotech). .. After completion of the dissociation program, the mixture was filtered successively through 100 μm and 30 μm cell strainers, and stained for 20min on ice in the dark with the following antibodies: 7-AAD (cat# 420403, 1:40, Biolegend), FITC-conjugated antibodies for anti-CD45 (cat# 130-116-500, clone 30F11, Miltenyi Biotech), anti-CD31 (cat# 130-123-675, clone 390, 1:100, Miltenyi Biotech), anti-CD11b (cat# 130-113-234, M1/ 70.15.11.5, 1:100, Miltenyi Biotech), APC-conjugated anti-Itgb1 (cat# 130- 123-829, clone HMβ1-1, 1:20, Miltenyi Biotech), PE-conjugated anti-Itga7 (cat# 130-120-812, clone 3C12, 1:100, Miltenyi Biotech), APC-Vio 770 antiPdgfra (cat# 130-126-625, clone APA5, 1:60, Miltenyi Biotech) and BV421 anti-Sca-1 (cat# 108128, clone D7, 1:100, Biolegend). .. MuSCs were isolated by FACS (BD FACSAria Fusion) using a gating strategy based on forward scatter and side scatter profiles and viability, negative for CD45, CD11b, CD31, Sca-1, Pdgfra and positive for Itgb1 and Itga7.

    Incubation:

    Article Title: Inhibition of HuR/ELAVL-1 attenuates fibrotic progression in Mdx mice with dilated cardiomyopathy.
    Article Snippet: .. CD45– cells were centrifuged, and the pellet was resuspended and incubated in Wash Buffer with FITC anti-mouse CD31 antibody (Miltenyi Biotec, Cat130-123-675). .. Cell suspension was then incubated in Wash Buffer containing anti-FITC MicroBeads and applied onto the LS column.

    other:

    Article Title: Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq
    Article Snippet: CD31 antibody, anti-mouse, Biotin (Dilutions in 1:50) , Miltenyi Biotec , Cat# 130-119-562, RRID: AB_2751728.



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    Image Search Results


    ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

    Journal: Science Advances

    Article Title: SIK2 antagonizes postinfarction cardiac repair by driving macrophage proinflammatory and impairing efferocytosis

    doi: 10.1126/sciadv.adw6624

    Figure Lengend Snippet: ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

    Article Snippet: Primary antibodies used to specifically detect the proteins of interest were as follows: SIK2 (6919s, CST), GAPDH (G9545, Sigma-Aldrich), α-SMA (A5228, Sigma-Aldrich), Collagen I (AB765P, Sigma-Aldrich), Collagen III (AB7778, Abcam), CD31 (3528s, CST), Phospho-NF-κB P65 (Ser 536 ) (3033s, CST), NF-κB P65 (8242s, CST), Phospho-P38 MAPK (Thr 180 /Tyr 182 ) (4511s, CST), P38 MAPK (8690s, CST), α-Tubulin (66031-1, Proteintech), Histone H3 (68345-1, Proteintech), NOS2 (13120s, CST), FLAG-Tag (14793s, CST), HA-Tag (3724s, CST), TRAF1 (4710s, CST), TRAF2 (4724s, CST), TRAF4 (18527s, CST), TRAF6 (67591s, CST), His-Tag (12698s, CST), and Myc-Tag (2276s, CST).

    Techniques: Analysis, Staining, Immunostaining, Tissue