Journal: Acta biomaterialia

Article Title: Peptide-dendrimer-reinforced bioinks for 3D bioprinting of heterogeneous and biomimetic in vitro models.

doi: 10.1016/j.actbio.2023.08.008

Figure Lengend Snippet: Fig. 10. (a) Immunostaining of the hepatic models against CD31 (green), F-actin (red), and DAPI (blue) at day 7 post bioprinting. (b) Albumin secretion. (c) Urea production. (d) Relative expression. (e) The enzyme activity of 3D bioprinted hepatic models. (f). Live/Dead staining of the hepatic models with drug treatment.

Article Snippet: Then, the samples were soaked with 0.1% riton-X 100 for 15 min and blocked with 10% goat serum alumin in PBST solution for 1 h. After that, the samples were ncubated with primary antibodies against CD31 (1:100, 6 60 65- -Ig, Proteintech) at 4 °C overnight.

Techniques: Immunostaining, Expressing, Activity Assay, Staining

Journal: Molecular medicine reports

Article Title: Swimming training promotes angiogenesis of endothelial progenitor cells by upregulating IGF1 expression and activating the PI3K/AKT pathway in type 2 diabetic rats.

doi: 10.3892/mmr.2024.13361

Figure Lengend Snippet: Figure 2. Morphology and identification of EPCs. (A) Characterization of EPCs from rats grown in vitro (magnification, x100). (B) Immunofluorescence staining with Dil‑ac‑LDL and/or FITC‑UEA‑I (scale bar, 20 µm). (C) Fluorescence microscopy detection of phenotypes CD31, CD34 and CD133 (scale bar, 20 µm). EPC, endothelial progenitor cell; Dil‑ac‑LDL, 1,1'‑dioctadecyl‑3,3,3', 3'‑tetramethylindocarbocyanine perchlorate‑labeled acetylated low‑density lipoprotein; FITC‑UEA‑I, fluorescein isothiocyanate‑conjugated Ulex europaeus agglutinin‑I.

Article Snippet: The primary antibodies were: CD31 (1:100; Proteintech Group; cat. no. CL488‐66065), CD34 (1:100; BIOSS; cat. no. bsm‐41197M) and CD133 (1:100; Proteintech Group; cat. no. 18470‐1‐AP).

Techniques: In Vitro, Immunofluorescence, Staining, Fluorescence, Microscopy

Journal: The Journal of Experimental Medicine

Article Title: The Semiconserved Head Structure of Plasmodium falciparum Erythrocyte Membrane Protein 1 Mediates Binding to Multiple Independent Host Receptors

doi:

Figure Lengend Snippet: Binding of Different Receptors and RBCs to var Fragment–transfected COS-7 Cells

Article Snippet: Soluble PECAM-1/CD31 purified from Chinese hamster ovary (CHO) cells (cat. no. ADP6) was purchased from R&D Systems.

Techniques: Binding Assay, Transfection