incubation with anti cd3 (Mabtech Inc)

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Mabtech Inc incubation with anti cd3
Incubation With Anti Cd3, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3/anti+cd3/pm42296188-71-1-5
Average 86 stars, based on 1 article reviews

incubation with anti cd3 - by Bioz Stars, 2026-10

86/100 stars

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Enzyme-linked Immunospot:

Article Title: Integrating complementary approaches reveals antigen-reactive CD4+ T cell states after SARS-CoV-2 vaccination
Article Snippet: .. ELISpot plates (Millipore, MSIPS4510) were coated with anti-human IFNg monoclonal antibody (clone 1-DIK, Mabtech, 3420-3-1000) at 0.5 μg/well overnight at 4◦C. .. Peripheral blood mononuclear cell (PBMC) isolation PBMCs were isolated from citrated peripheral blood by density gradient centrifugation using a BioColl density medium with a density of 1.077 g/mL (BioSell, BS.L 6115).

Article Title: Extensive evolution and T cell escape by SARS-CoV-2 in a 2.5-year persistent infection of an immunocompromised host
Article Snippet: .. ELISpot biotinylated IFNγ detection antibodies clone 7-B6-1 , Mabtech , 3420-6. .. ELISpot coating antibody human anti-IFNγ clone D1K , Mabtech , 3420-3.

Article Title: Extensive evolution and T cell escape by SARS-CoV-2 in a 2.5-year persistent infection of an immunocompromised host
Article Snippet: ELISpot biotinylated IFNγ detection antibodies clone 7-B6-1 , Mabtech , 3420-6. .. ELISpot coating antibody human anti-IFNγ clone D1K , Mabtech , 3420-3. .. goat anti-biotin alkaline phosphatase , Vector Laboratories , SP-3020.

Recombinase Polymerase Amplification:

Article Title: Decoding the human CD4 + T cell epitope repertoire for Lassa fever virus reveals T cell-based pan-mammarenavirus vaccine candidates.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD3 Alexa Fluor 700 (clone SP34-2) BD Biosciences Cat# 557917; RRID: AB_396938 Mouse anti-human CD3 BUV395 (clone UCHT1) BD Biosciences Cat# 563546; RRID: AB_2744387 Mouse anti-human CD4 APC-eFlour 780 (clone RPA-T4) ThermoFisher Cat# 47-0049-42; RRID: AB_1272044 Mouse anti-human CD4 BV605 (clone RPA-T4) BD Biosciences Cat# 562658; RRID: AB_2744420 Mouse anti-human CD8a Brilliant Violet 650 (clone RPA-T8) BioLegend Cat# 301042; RRID: AB_2563505 Mouse anti-human CD8 BUV496 (clone RPA-T8) BD Biosciences Cat# 612942; RRID: AB_2870223 Mouse anti-human CD69 BV786 (clone FN50) BD Biosciences Cat# 563834; RRID: AB_2738441 Mouse anti-human OX40 PE-Cyanine7 (clone Ber-ACT35) BioLegend Cat# 350012; RRID: AB_10901161 Mouse anti-human CD137 APC (clone 4B4-1) BioLegend Cat# 309810; RRID: AB_830672 Mouse anti-human IFN-γ FITC (clone 4S.B3) ThermoFisher Cat# 11-7319-82; RRID: AB_465415 Live/Dead Fixable Near IR (780) Viability Invitrogen Cat# L34994 Fixable Viability Dye eFlour 506 ThermoFisher Cat# 65-0866-18 Anti-human IFN-γ mAb, unconjugated (clone 1-D1K) Mabtech Cat# 3420-3-1000 FluoroSpot: Human IFN-γ FluoroSpot (490) Mabtech Cat# FSP-01A-10 FluoroSpot: Mouse IFN-γ FluoroSpot Flex Set Mabtech Cat# FSX-41A-10 Biological samples LCMV infected blood samples Kotturi et al.52 N/A LASV-GPC vaccinated blood samples Emergent BioSolutions N/A Blood samples from healthy donors LJI Clinical Core, CTRI-La Jolla, CTC-Hillcrest, CSG-Miami, Carter Bloodcare-DAL N/A Chemicals, peptides, and recombinant proteins Synthetic peptides TC Peptide Lab https://tcpeptidelab.com Brilliant Staining Buffer Plus BD Biosciences Cat# 566385; RRID: AB_2869761 Cyto-Fast Fix/Perm Buffer Set BioLegend Cat# 426803 AddaVax adjuvant InvivoGen Cat# vac-adx-10 Critical commercial assays Lymphocytic Choriomeningitis (LCM) Virus Antibody, IFA, Serum Focus Diagnostics Cat# 34345 (Continued on next page) e1 Cell Reports Medicine 7, 102824, June 16, 2026 Article ll OPEN ACCESS ..

Infection:

Recombinant:

Staining:

Adjuvant:

Laser Capture Microdissection:

Virus:

Immunofluorescence:

other:

Article Title: Integrating complementary approaches reveals antigen-reactive CD4 + T cell states after SARS-CoV-2 vaccination
Article Snippet: For the viability staining, ethidium-monoazide-bromide (EMA; ThermoFisher, E1374), propidium iodide (PI; ThermoFisher, P1304MP), or ZombieNIR Fixable Viability Kit (BioLegend, 423106, 1:1000) was used.

Activation Assay:

Article Title: UNICOR-v, a Pan-Coronavirus Subunit Vaccine, Demonstrates Immunogenicity and Efficacy Against MERS-CoV Infection.
Article Snippet: FLUOROSpot Splenocytes (4 × 105 cells/well) were added to 96-well FLUOROSpot plates (MabTech, Nacka Strand, Sweden) pre-coated with IFN-g, IL-2, and TNF-a capture antibodies, as per the manufacturer’s instructions, and incubated with a pool of the 12 peptides (2 mM of each peptide) in complete media (RPMI-1680 media +10% FCS, media alone (negative control), or 5 ug/mL Concanavalin A (Sigma-Aldrich, Gillingham, UK) in complete media. .. Anti-CD28 antibody (MabTech, Nacka Strand, Sweden) was added to provide the necessary co-stimulatory molecules to support T-cell activation. ..

Blocking Assay:

Article Title: Comparing CD8+ T cell ENV epitopes of two HLA class I alleles associated with different outcomes of HIV-1 infection with broadly neutralizing antibodies epitope targets discovered to date
Article Snippet: .. Ninety-six well nitrocellulose plates were coated with anti-IFN-γ monoclonal antibody (mAb; Mabtech, Nacka Strand, Sweden) followed by blocking with R-10 media. ..

Incubation:

Article Title: Comparative Long-Term Immunogenicity and Boosting of 3-Versus 4-Session Intradermal Rabies Post-Exposure Prophylaxis Regimens, Cambodia and Madagascar: The Resist-3 Study.
Article Snippet: [29] Fluorospot IFN-γ/IL-4 Assay Rabies-specific T cell response was evaluated by the FluoroSpot Plus: Human IFN-γ/IL-4 kit (Mabtech) using a peptide pool called GlyRab, which is derived from 103 peptides covering each peptide of the full rabies glycoprotein with an overlap of 10 residues, 1 μg/mL of each 15-mer peptide, purity > 80%, (Mimotopes, Victoria, Australia). .. [13] Incubation with anti-CD3 (CD3-2, Mabtech) was used as a positive control, complete RPMI-160 Medium (SIGMA Life Sciences) only as a negative control (volume matched with peptide added), and complete RPMI-160 Medium (SIGMA Life Sciences) without cells as a background control. ..

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