Journal: bioRxiv

Article Title: Synergistic Hypoxia and Apoptosis Conditioning Unleashes Superior Mesenchymal Stem Cells Efficacy in Acute Graft-versus-Host-Disease

doi: 10.1101/2024.04.11.588248

Figure Lengend Snippet: Effect of hypoxia-preconditioned MSCs (MSCs HYP , MSCs HYP→APO , MSCs HYP+APO ) on the proliferation of aGVHD patients derived T-cell. The bar graph represents (A) the percentage of CD3 + CFSE + proliferating T-cell (n=5). (B) the ratio of CD4 + /CD8 + T cell (n=5) in the direct co-culture of MSCs and T-cell. Data shown as Mean±S.D.; Statistical analysis: Tukey’s multiple comparisons test; **≤0.01; ****≤0.0001. Abbreviations: BM: Bone marrow; WJ: Wharton’s Jelly; MSCs: Mesenchymal Stem Cells; HYP: Hypoxia-preconditioned; APO: Apoptosis .

Article Snippet: CD3 + T-cell was isolated from PBMNCs by negative selection using a Pan T cell isolation kit, human (Miltenyi Biotec, USA) as per the manufacturer’s instructions.

Techniques: Derivative Assay, Co-Culture Assay

Journal: bioRxiv

Article Title: Synergistic Hypoxia and Apoptosis Conditioning Unleashes Superior Mesenchymal Stem Cells Efficacy in Acute Graft-versus-Host-Disease

doi: 10.1101/2024.04.11.588248

Figure Lengend Snippet: Dot plots showing the proliferation of CD3 + T-cell in the direct co-culture of aGVHD patients derived T-cell and (A) Untreated (Control). (B) BM-MSCs HYP . (C) BM-MSCs HYP→APO . (D) BM-MSCs HYP+APO . (E) WJ-MSCs HYP . (F) WJ-MSCs HYP→APO . (G) WJ-MSCs HYP+APO . Abbreviations: BM: Bone marrow; WJ: Wharton’s Jelly; MSCs: Mesenchymal Stem Cells; HYP: Hypoxia-preconditioned; APO: Apoptosis .

Article Snippet: CD3 + T-cell was isolated from PBMNCs by negative selection using a Pan T cell isolation kit, human (Miltenyi Biotec, USA) as per the manufacturer’s instructions.

Techniques: Co-Culture Assay, Derivative Assay, Control

Journal: bioRxiv

Article Title: Synergistic Hypoxia and Apoptosis Conditioning Unleashes Superior Mesenchymal Stem Cells Efficacy in Acute Graft-versus-Host-Disease

doi: 10.1101/2024.04.11.588248

Figure Lengend Snippet: Effect of hypoxia-preconditioned MSCs (MSCs HYP , MSCs HYP→APO , MSCs HYP+APO ) on the induction of Tregs and polarization of helper T cell from pro-inflammatory (Th1, Th17) to anti-inflammatory (Th2) phenotype. The bar graph represents (A) the induction of CD3 + CD4 + CD25 + FOXP3 + Tregs (n=5). (B) the ratio of Th1/Th2 (n=5). (C) Th1/Th17 ratio (n=5) in the direct co-culture of MSCs and T-cell. Data shown as Mean±S.D.; Statistical analysis: Tukey’s multiple comparisons test; ***≤0.001; ****≤0.0001. Abbreviations: BM: Bone marrow; WJ: Wharton’s Jelly; MSCs: Mesenchymal stem cells; HYP: Hypoxia-preconditioned; APO: Apoptosis, Tregs: Regulatory helper T-cell, Th: Helper T cell .

Article Snippet: CD3 + T-cell was isolated from PBMNCs by negative selection using a Pan T cell isolation kit, human (Miltenyi Biotec, USA) as per the manufacturer’s instructions.

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Synergistic Hypoxia and Apoptosis Conditioning Unleashes Superior Mesenchymal Stem Cells Efficacy in Acute Graft-versus-Host-Disease

doi: 10.1101/2024.04.11.588248

Figure Lengend Snippet: Dot plots showing the percentage of CD3 + CD4 + CD25 + FOXP3 + in the direct co-culture of aGVHD patients derived CD3 + T-cell and (A) Untreated (Control). (B) BM-MSCs HYP . (C) BM-MSCs HYP→APO . (D) BM-MSCs HYP+APO . (E) WJ-MSCs HYP . (F) WJ-MSCs HYP→APO . (G) WJ-MSCs HYP+APO . Abbreviations: BM: Bone marrow; WJ: Wharton’s Jelly; MSCs: Mesenchymal Stem Cells; HYP: Hypoxia-preconditioned; APO: Apoptosis .

Article Snippet: CD3 + T-cell was isolated from PBMNCs by negative selection using a Pan T cell isolation kit, human (Miltenyi Biotec, USA) as per the manufacturer’s instructions.

Techniques: Co-Culture Assay, Derivative Assay, Control

Journal: Biomarker research

Article Title: Overexpressed immunoglobulin-like transcript (ILT) 4 in lung adenocarcinoma is correlated with immunosuppressive T cell subset infiltration and poor patient outcomes.

doi: 10.1186/s40364-020-00191-7

Figure Lengend Snippet: Fig. 2 Overexpressed ILT4 in tumor cells was associated with decreased T cell infiltration in LUAD patients. a & b ILT4-high group displayed fewer CD3+T lymphocyte infiltration compared with the ILT4-low counterpart by immunohistochemical staining. a showed the typical images of ILT4 expression and infiltrating CD3+T cell density. Each paraffin-embedded tissue was sequentially sectioned for ILT4 or CD3 staining respectively. b showed the statistical result in all patients (p < 0.001). Scale bar:20 μm. c Low CD3+T cell frequency in TILs indicated poorer patient OS (p < 0.001). The cutoff value for high and low CD3 frequency were defined as ≥median and < median respectively. d High ILT4 expression in tumor cells was correlated with reduced CD3+T cell proportion both in the cancer nest (p = 0.003) and stroma (p < 0.001). The cutoff scores for high and low ILT4 expression were the same as in Fig. 1c

Article Snippet: The primary antibodies were as follows: anti-ILT4 antibody (1:25, Origene, Cat#TA349368), anti-CD3 antibody (1100, Proteintech, Cat#17617–1-AP), anti-CD4 antibody (1100, Abcam, Cat#ab133616), anti-CD8 antibody (ready-to-use, Zhongshan Jinqiao Biotechnology Co. Ltd., Cat#ZA0508), anti-FoxP3 antibody (1,25, Abcam, Cat#ab20034).

Techniques: Immunohistochemical staining, Staining, Expressing