cd19 car detection reagent (Miltenyi Biotec)
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Miltenyi Biotec
cd19 car detection reagent
Cd19 Car Detection Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd19/CD19+CAR+Detection+Reagent%2C+human/pmc13389840-46-5-9
Average 96 stars, based on 122 article reviews
Cd19 Car Detection Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd19/CD19+CAR+Detection+Reagent%2C+human/pmc13389840-46-5-9
Average 96 stars, based on 122 article reviews
cd19 car detection reagent - by Bioz Stars,
2026-11
96/100 stars
Images
1) Product Images from "Coexpression of GITRL confers resistance to Treg cell-mediated immunosuppression to anti-CD19 CAR-NK cells"
Article Title: Coexpression of GITRL confers resistance to Treg cell-mediated immunosuppression to anti-CD19 CAR-NK cells
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2026.1818610
Figure Legend Snippet: Generation of CAR19-NK-92 and CAR19-GITRL-NK-92 cells. (A) SFFV-driven CAR19 and CAR19-GITRL constructs: FMC63 anti-CD19 scFv linked to CD28 transmembrane and CD28/CD3ζ signaling domains; CAR19-GITRL includes a P2A sequence followed by GITRL, whereas CAR19 contains a stop codon. (B) NK-92 cells were transduced with VSV-G–pseudotyped lentiviral particles (MOI 10) in the presence of polybrene (8 μg/mL) and BX795 (16 μM); CAR expression was assessed by flow cytometry. (C) CAR-positive cells were enriched by FACS (FACSAria Fusion), and CAR, GITR, and GITRL expression were quantified by flow cytometry.
Techniques Used: Construct, Sequencing, Transduction, Expressing, Flow Cytometry
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Dynabeads M-280 Streptavidin (Invitrogen, Thermo Scientific, Waltham, MA, USA) were loaded with Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production. Article Snippet: Cells were detached with trypsin-EDTA, washed with PBS, and resuspended in 1 mL of MACSQuant Running Buffer for cytometry (RB, Miltenyi Biotec Cat. 130-092-747). .. For the LV/CAR vector, non-permeabilized cells were stained by sequential incubation with Saline:Article Title: A versatile and GMP-compliant bead-based IFN-γ potency assay for standardized quality control and release testing of CAR-T cell drug products Article Snippet: .. Dynabeads M-280 Streptavidin (Invitrogen, Thermo Scientific, Waltham, MA, USA) were loaded with Control:Article Title: A versatile and GMP-compliant bead-based IFN-γ potency assay for standardized quality control and release testing of CAR-T cell drug products Article Snippet: .. Dynabeads M-280 Streptavidin (Invitrogen, Thermo Scientific, Waltham, MA, USA) were loaded with FACS:Article Title: Safe Redosable Low-Immunogenic In Vivo CAR-T Therapy for B Cell Malignancies and Solid Tumors Article Snippet: .. Cells were analyzed on BD FACS Aria, Accuri, Lyric, or Beckman Coulter CytoFLEX instruments and data analyzed using FlowJo v10 or CytExpert software as described previously., CAR transgene expression was detected using CAR-specific detection reagents: CD19 CAR detection (Miltenyi Biotec #130-129-550), Article Title: Engineered mRNA backbones for gene expression in human T cells Article Snippet: .. Cells were harvested 24–48 h post-electroporation, washed in FACS buffer (PBS with 2% FBS), and stained with the following fluorochrome-conjugated antibodies: Software:Article Title: Safe Redosable Low-Immunogenic In Vivo CAR-T Therapy for B Cell Malignancies and Solid Tumors Article Snippet: .. Cells were analyzed on BD FACS Aria, Accuri, Lyric, or Beckman Coulter CytoFLEX instruments and data analyzed using FlowJo v10 or CytExpert software as described previously., CAR transgene expression was detected using CAR-specific detection reagents: CD19 CAR detection (Miltenyi Biotec #130-129-550), Expressing:Article Title: Safe Redosable Low-Immunogenic In Vivo CAR-T Therapy for B Cell Malignancies and Solid Tumors Article Snippet: .. Cells were analyzed on BD FACS Aria, Accuri, Lyric, or Beckman Coulter CytoFLEX instruments and data analyzed using FlowJo v10 or CytExpert software as described previously., CAR transgene expression was detected using CAR-specific detection reagents: CD19 CAR detection (Miltenyi Biotec #130-129-550), Bioprocessing:Article Title: Safe Redosable Low-Immunogenic In Vivo CAR-T Therapy for B Cell Malignancies and Solid Tumors Article Snippet: .. Cells were analyzed on BD FACS Aria, Accuri, Lyric, or Beckman Coulter CytoFLEX instruments and data analyzed using FlowJo v10 or CytExpert software as described previously., CAR transgene expression was detected using CAR-specific detection reagents: CD19 CAR detection (Miltenyi Biotec #130-129-550), Construct:Article Title: Safe Redosable Low-Immunogenic In Vivo CAR-T Therapy for B Cell Malignancies and Solid Tumors Article Snippet: .. Cells were analyzed on BD FACS Aria, Accuri, Lyric, or Beckman Coulter CytoFLEX instruments and data analyzed using FlowJo v10 or CytExpert software as described previously., CAR transgene expression was detected using CAR-specific detection reagents: CD19 CAR detection (Miltenyi Biotec #130-129-550), Staining:Article Title: Engineered mRNA backbones for gene expression in human T cells Article Snippet: .. Cells were harvested 24–48 h post-electroporation, washed in FACS buffer (PBS with 2% FBS), and stained with the following fluorochrome-conjugated antibodies: Article Title: Coexpression of GITRL confers resistance to Treg cell-mediated immunosuppression to anti-CD19 CAR-NK cells Article Snippet: .. CAR-positive cells were stained with Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production. Article Snippet: Cells were detached with trypsin-EDTA, washed with PBS, and resuspended in 1 mL of MACSQuant Running Buffer for cytometry (RB, Miltenyi Biotec Cat. 130-092-747). .. For the LV/CAR vector, non-permeabilized cells were stained by sequential incubation with Plasmid Preparation:Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production. Article Snippet: Cells were detached with trypsin-EDTA, washed with PBS, and resuspended in 1 mL of MACSQuant Running Buffer for cytometry (RB, Miltenyi Biotec Cat. 130-092-747). .. For the LV/CAR vector, non-permeabilized cells were stained by sequential incubation with |
