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Image Search Results
Journal: Arthritis and rheumatism
Article Title: Engraftment of PBMC from SLE and APS Donors into BALB-Rag2-/-IL2Rgc-/- Mice: a Promising Model for Studying Human Disease
doi: 10.1002/art.30424
Figure Lengend Snippet: Immunohistochemistry and histology of the Kidneys of SLE–DKO, ND-DKO, and un-engrafted DKO mice. A. Human IgG deposits were present in the glomeruli of P1, P3 and P5 SLE-DKO kidneys, and absent in the ND-DKO and un-engrafted DKO kidneys. B. H & E and PAS stains of kidneys of P5-DKO, ND-DKO, and DKO mice. There was abundant perivascular infiltrate of human cells in SLE-DKO kidney, compared to the less prominent infiltration in ND-DKO kidney. The DKO kidney displayed no infiltrate. In the kidney of P5-DKO mouse, the glomeruli were swollen with cellular necrosis. The hyaline thrombi present in the glomerular capillary loops and capillaries (white arrows) were evident on PAS stain. Glomeruli of both the ND-DKO and DKO mice appeared normal C. CD3+, CD8+ and CD19+ human cells were present in the glomeruli of P5-DKO, scanty in ND-DKO, and absent in DKO kidney.
Article Snippet: Antibody to: CD3 (rabbit monoclonal antibody, Vector Lab, CA), CD8 (mouse monoclonal antibody, Dako, CA) and
Techniques: Immunohistochemistry, Staining