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cd14+ monocyte 2w400a  (Lonza)


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    Structured Review

    Lonza cd14+ monocyte 2w400a
    Cd14+ Monocyte 2w400a, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14++monocyte+2w400a/cd14++monocytes/pm39890818-65-0-6
    Average 90 stars, based on 1 article reviews
    cd14+ monocyte 2w400a - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Migration:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Control:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Transfection:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Purification:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    RNA Sequencing:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Irradiation:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Incubation:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Software:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Sequencing:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.

    Concentration Assay:

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: Human peripheral blood CD14+ monocytes were procured from Lonza and maintained in RPMI-1640 supplemented with heat-inactivated 10% fetal bovine serum (Biotechne, screened for MDM maturation and viability), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM glutamine, and 5 ng/mL GM-CSF or 20 ng/mL M-CSF (Peprotech).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: CD14+ monocytes positively selected from large-scale apheresis on healthy human donors (Lonza) were differentiated into macrophages in low-attachment bioprocess bags (Saint-Gobain Performance Plastics) for 9 days in RPMI-1640 medium (Gibco 11875093) supplemented with 10% Fetal Bovine Serum (Gibco 10082139), 10 mM HEPES (Gibco 15630080), 1× Penicillin-Streptomycin (Gibco 15140122), 1× Non-essential amino acids (Gibco 11140050), 1 mM Sodium Pyruvate (Gibco 11360070), 1× GlutaMAX (Gibco 35050-061), and 50 ng/mL M-CSF (Promocell C-60442A).

    Article Title: Therapeutic uses of anti-GITR antibodies
    Article Snippet: Frozen CD14+ cells obtained from Lonza were thawed, resuspended in cell culture media (RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 292 μg/mL L-glutamine, 1% human AB serum, NaPyr (sodium pyruvate), HEPES, NEAA (non-essential amino acids), and 0.01 mM beta-mercaptoethanol) supplemented with 50 ng/mL GM-CSF, and plated at 5×104 cells/well into clear-bottom, collagen-coated 96-well black plates for differentiation into phagocytes over 13 days, with fresh GM-CSF (50 ng/ml) added on day 6 and day 12.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: Human Peripheral Blood CD14+ Monocytes (Lonza, #2W-400C) were seeded in a 6-well culture plate at a concentration 1x106 /well in 2 mL RPMI medium (Life technologies, #31870- 074) containing 10mM, 1mM sodium pyruvate, GlutaMAX, penicillin/streptomycin and 10% FBS supplemented with 50 ng/mL GM-CSF (Peprotech, #300-03-20UG) or 50 ng/mL M-CSF Jo urn l P re- pro of (Peprotech, # 300-25) for IFN-γ/LPS-induced and IL4-induced macrophage polarization, respectively and finally incubated at 37oC and 5% CO2.

    Article Title: Mucosal macrophages govern intestinal regeneration in response to injury.
    Article Snippet: For co-149 culture with IFN-γ/LPS-induced or IL-4-induced macrophages, human peripheral blood CD14+ 150 monocytes (Lonza) was used.

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection.
    Article Snippet: Human peripheral blood CD14+ monocyte (2W400A, Lonza)-derived macrophages were generated as previously described [30].

    Article Title: Local, but not circulating, complement C3 governs immune checkpoint blockade efficacy
    Article Snippet: Primary human CD14 + monocytes were purchased from Lonza (2W-400B, batch numbers 19TL113254 and 22TL108493).

    Article Title: The synthetic opioid fentanyl increases HIV replication in macrophages.
    Article Snippet: PLOS ONE | https://doi.org/10.1371/journal.pone.0298341 February 27, 2025 3 / 17 Human peripheral blood CD14+ monocytes were obtained from Lonza and cultured in RPMI 1640.



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