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ecl chemiluminescent reagents  (Boster Bio)


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    Boster Bio ecl chemiluminescent reagents
    Ecl Chemiluminescent Reagents, supplied by Boster Bio, used in various techniques. Bioz Stars score: 98/100, based on 716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/Exodus-2+Human+Recombinant+Protein/pmc12990274-52-7-11
    Average 98 stars, based on 716 article reviews
    ecl chemiluminescent reagents - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: MCUB restricts mitochondrial calcium to support metabolic adaptation and homeostasis in glioblastoma.
    Article Snippet: .. Immunoreactive bands were visualized using an ECL chemiluminescence detection kit (BOSTER) and captured with a Bio-Rad imaging system. ..

    Article Title: miR-142-3p overexpression targets CLTA and impairs clathrin-mediated endocytosis in HLSECs induced by high glucose.
    Article Snippet: 1 The First Clinical Medical College of Gansu University of Chinese Medicine, Lanzhou, Gansu, China 2 Department of Gerontology, Gansu Provincial Hospital, Lanzhou, Gansu, China 3 The Third Clinical College of Lanzhou University, Lanzhou, Gansu, China Abstract Objective This study aims to explore the effect of miR-142-3p on the endocytosis in human liver sinusoidal endothelial cells (HLSECs) induced by high glucose and to investigate the underlying mechanisms.. Methods Multi-database target prediction was performed to identify potential targets of hsa-miR-142-3p.. HLSECs were cultured under 5.5 mmol/L glucose (normal) or 25 mmol/L glucose (high) conditions.

    Membrane:

    Article Title: Chinese bayberry leaves proanthocyanidins regulate glucose and lipid metabolism associated with the modulation of the gut microbiota-barrier axis
    Article Snippet: .. Apply ECL chemiluminescent substrate solution (BOSTER, China) onto the membrane and incubate in the dark for 2 min to initiate the chemiluminescent reaction. .. Use a chemiluminescence imaging system (Bio-Rad, China) to visualize the protein bands and capture images.

    Article Title: miR-142-3p overexpression targets CLTA and impairs clathrin-mediated endocytosis in HLSECs induced by high glucose.
    Article Snippet: 1 The First Clinical Medical College of Gansu University of Chinese Medicine, Lanzhou, Gansu, China 2 Department of Gerontology, Gansu Provincial Hospital, Lanzhou, Gansu, China 3 The Third Clinical College of Lanzhou University, Lanzhou, Gansu, China Abstract Objective This study aims to explore the effect of miR-142-3p on the endocytosis in human liver sinusoidal endothelial cells (HLSECs) induced by high glucose and to investigate the underlying mechanisms.. Methods Multi-database target prediction was performed to identify potential targets of hsa-miR-142-3p.. HLSECs were cultured under 5.5 mmol/L glucose (normal) or 25 mmol/L glucose (high) conditions.

    Article Title: Tumor-derived extracellular vesicles exert promotional effects on metastasis in renal cell carcinoma through the delivery of microRNA-671-5p.
    Article Snippet: Purpose Tumor-derived extracellular vesicles (EVs) can deliver microRNAs (miRNAs) to promote tumor development.. Herein, this study explored whether tumor-derived EVs carrying miR-671-5p facilitated renal cell carcinoma (RCC) cell growth.. Methods miR-671-5p expression in RCC cells and the collected EVs was assessed.

    Incubation:

    Article Title: Tumor-derived extracellular vesicles exert promotional effects on metastasis in renal cell carcinoma through the delivery of microRNA-671-5p.
    Article Snippet: Purpose Tumor-derived extracellular vesicles (EVs) can deliver microRNAs (miRNAs) to promote tumor development.. Herein, this study explored whether tumor-derived EVs carrying miR-671-5p facilitated renal cell carcinoma (RCC) cell growth.. Methods miR-671-5p expression in RCC cells and the collected EVs was assessed.



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    Image Search Results


    A Experimental design. Primary human CD8⁺ T cells were co-engineered with a lentiviral construct encoding the NR4A2-responsive synthetic promoter SP73 driving mCherry and constitutive EGFP to identify transduced cells, together with a retroviral NY-ESO-1–specific TCR. Engineered T cells were expanded and co-cultured with NY-ESO-1–expressing SK-MEL23 melanoma cells. B Quantification of mCherry⁺ T cells over time during co-culture. SP73-engineered T cells show progressive reporter induction, whereas control cells transduced with the control promoter SP01 (lacking NR4A2 binding sites) remain at baseline levels. Data represent mean ± SEM of n = 3 independent donors. C Representative IncuCyte® live-cell images showing SP73-driven mCherry fluorescence during co-culture with SK-MEL23 melanoma cells; SP01 controls show negligible signal. D Expression of NR4A2 and activation/dysfunction-associated markers in engineered T cells during co-culture. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. P values: CD39, P < 0.0001 for R0vsR2 and R0vsR4, P = 0.0048 for R2vsR4. NR4A2, P = 0.0375 for R0vsR2. LAG-3, P = 0.0194 for R0vsR2, P = 0.0097 for R0vsR4. TIM-3, P = 0.008 for R0vsR2, P = 0.0433 for R2vsR4. TIGIT, P = 0.0082 for R0vsR4, P = 0.0433 for R2vsR4. E Functional assessment of engineered T cells prior co-culture (R0) and following persistent antigen exposure (R4). Left: Quantification of IL-2⁺, TNFα⁺, CD107a⁺, and IFNγ⁺ cells following Leukocyte Activation Cocktail (LAC) stimulation. Right: Representative flow cytometry histograms illustrating shifts in cytokine-producing populations. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using a two-tailed paired t test comparing R0 vs R4. P values: IL-2, P = 0.0074; TNFα, P = 0.0068; CD107a, P = 0.0401; IFNγ, P = 0.0147. Source data for this figure is available in the file. Created in BioRender.

    Journal: Nature Communications

    Article Title: A modular synthetic promoter toolkit for programmable gene control in activated and chronically stimulated T cells

    doi: 10.1038/s41467-026-77730-7

    Figure Lengend Snippet: A Experimental design. Primary human CD8⁺ T cells were co-engineered with a lentiviral construct encoding the NR4A2-responsive synthetic promoter SP73 driving mCherry and constitutive EGFP to identify transduced cells, together with a retroviral NY-ESO-1–specific TCR. Engineered T cells were expanded and co-cultured with NY-ESO-1–expressing SK-MEL23 melanoma cells. B Quantification of mCherry⁺ T cells over time during co-culture. SP73-engineered T cells show progressive reporter induction, whereas control cells transduced with the control promoter SP01 (lacking NR4A2 binding sites) remain at baseline levels. Data represent mean ± SEM of n = 3 independent donors. C Representative IncuCyte® live-cell images showing SP73-driven mCherry fluorescence during co-culture with SK-MEL23 melanoma cells; SP01 controls show negligible signal. D Expression of NR4A2 and activation/dysfunction-associated markers in engineered T cells during co-culture. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. P values: CD39, P < 0.0001 for R0vsR2 and R0vsR4, P = 0.0048 for R2vsR4. NR4A2, P = 0.0375 for R0vsR2. LAG-3, P = 0.0194 for R0vsR2, P = 0.0097 for R0vsR4. TIM-3, P = 0.008 for R0vsR2, P = 0.0433 for R2vsR4. TIGIT, P = 0.0082 for R0vsR4, P = 0.0433 for R2vsR4. E Functional assessment of engineered T cells prior co-culture (R0) and following persistent antigen exposure (R4). Left: Quantification of IL-2⁺, TNFα⁺, CD107a⁺, and IFNγ⁺ cells following Leukocyte Activation Cocktail (LAC) stimulation. Right: Representative flow cytometry histograms illustrating shifts in cytokine-producing populations. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using a two-tailed paired t test comparing R0 vs R4. P values: IL-2, P = 0.0074; TNFα, P = 0.0068; CD107a, P = 0.0401; IFNγ, P = 0.0147. Source data for this figure is available in the file. Created in BioRender.

    Article Snippet: The sequence of IL12p70 was obtained from pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe) (Addgene #105201 ).

    Techniques: Construct, Retroviral, Cell Culture, Expressing, Co-culture Assay, Control, Transduction, Binding Assay, Fluorescence, Activation Assay, Analysis, Functional Assay, Flow Cytometry, Two Tailed Test