ccl21 Search Results


mouse ccl21 6ckine kit  (R&D Systems)
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R&D Systems mouse ccl21 6ckine kit
Mouse Ccl21 6ckine Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecl chemiluminescent reagents  (Boster Bio)
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Boster Bio ecl chemiluminescent reagents
Ecl Chemiluminescent Reagents, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ccl21  (R&D Systems)
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Human Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse ccl21 6ckine duoset  (R&D Systems)
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cycloheximide treated hela 229 cell monolayers  (ATCC)
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ATCC cycloheximide treated hela 229 cell monolayers
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ccl21 goat polyclonal  (R&D Systems)
93
R&D Systems ccl21 goat polyclonal
FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and <t>CCL21.</t> (B) Quantification of <t>CCL21</t> immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.
Ccl21 Goat Polyclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse recombinant ccl21  (R&D Systems)
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R&D Systems mouse recombinant ccl21
FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and <t>CCL21.</t> (B) Quantification of <t>CCL21</t> immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.
Mouse Recombinant Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ccl21  (R&D Systems)
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R&D Systems anti ccl21
FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and <t>CCL21.</t> (B) Quantification of <t>CCL21</t> immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.
Anti Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ccl21 6ckine  (R&D Systems)
91
R&D Systems human ccl21 6ckine
FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and <t>CCL21.</t> (B) Quantification of <t>CCL21</t> immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.
Human Ccl21 6ckine, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab 610 human tnf mouse r d systems pc3 188a human cd3 mouse dako af366 human ccl21 goat r d systems 1f8 human cd21 mouse dako l  (R&D Systems)
93
R&D Systems mab 610 human tnf mouse r d systems pc3 188a human cd3 mouse dako af366 human ccl21 goat r d systems 1f8 human cd21 mouse dako l
FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and <t>CCL21.</t> (B) Quantification of <t>CCL21</t> immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.
Mab 610 Human Tnf Mouse R D Systems Pc3 188a Human Cd3 Mouse Dako Af366 Human Ccl21 Goat R D Systems 1f8 Human Cd21 Mouse Dako L, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela cells  (ATCC)
94
ATCC hela cells
FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and <t>CCL21.</t> (B) Quantification of <t>CCL21</t> immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.
Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and CCL21. (B) Quantification of CCL21 immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.

Journal: Frontiers in aging

Article Title: Structural and Functional Changes in Aged Skin Lymphatic Vessels.

doi: 10.3389/fragi.2022.864860

Figure Lengend Snippet: FIGURE 6 | Aging causes decreased chemokine and altered junctional protein expression by LECs. (A) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and CCL21. (B) Quantification of CCL21 immunostaining as a percentage of lymphatic vessel area. (C) qRT-PCR quantification of CCL21 mRNA expression in sorted skin LECs. (D) Representative confocal images of ear skin wholemount stained for nuclei (DAPI), VE-cadherin (upper panel, Initial lymphatics; lower, collecting lymphatics). (E) qRT-PCR quantification of VE-cadherin and (F) ZO-1 mRNA expression in sorted skin LECs. (G) Representative confocal images of ear skin sections stained for lymphatic vessels (LYVE-1) and ICAM-1 (upper panel, immunofluorescence of both; lower, ICAM-1 only). (H) qRT-PCR quantification of mRNA expression of endothelial adhesion molecules ICAM-1 and (I) p-Selectin from sorted skin LECs. n = 5 mice from each group. All quantifications are mean ± SD, unpaired Student’s t test.

Article Snippet: Antigen Antibody type Dilution Catalog number Company LYVE-1 Goat polyclonal 1:400 2125-LY R&D Systems (Minneapolis, MN) VE-cadherin Goat polyclonal 1:500 AF1002 R&D Systems LYVE-1 Rabbit polyclonal 1:200 Ab14917 Abcam (Cambridge, MA) Podoplanin Hamster monoclonal 1:500 Ab11936 Abcam α-SMA Mouse monoclonal Cy3-conjugated 1:1000 C6198 Sigma-Aldrich (Saint Louis, MO) CD31 Rat monoclonal 1:200 553370 BD Biosciences (Franklin Lakes, NJ) CD11b Rat monoclonal 1:300 557395 BD Biosciences VEGFR3/Flt-4 Goat polyclonal 1:100 AF743 R&D Systems pAkt Rabbit monoclonal 1:400 4060s R&D Systems CCL21 Goat polyclonal 1:40 AF457 R&D Systems Cleaved Caspase-3 Rabbit monoclonal 1:100 MAB 835 R&D Systems VE-Cadherin Goat polyclonal 1:500 AF1002 R&D Systems ICAM-1 Rat monoclonal 1:100 YN1/1.7.4 Ab119871 Abcam p-Selectin Rabbit monoclonal 1:500 EPR5047 Ab134047 Abcam iNOS Rabbit polyclonal 1:100 Ab3523 Abcam CD3 Rabbit polyclonal 1:200 A0452 Dako (Santa Clara, CA) Nitrotyrosine (N-Tyr) Rabbit polyclonal 1:200 BS-8551R BIOSS (Woodburn, MA) Collagen I Rabbit polyclonal 1:100 Ab34710 Abcam MacroH2A1 Rabbit polyclonal 1:100 39593 Thermo Fisher RYR1 Rabbit polyclonal 1:200 AB9078 Millipore Frontiers in Aging | www.frontiersin.org April 2022 | Volume 3 | Article 8648603 the dorsal hindlimbs of each mouse.

Techniques: Expressing, Staining, Immunostaining, Quantitative RT-PCR