ccl21 (R&D Systems)
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ccl21
Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ccl21/Mouse+CCL21%2F6Ckine+Antibody/pmc12343296-253-28-34
Average 93 stars, based on 90 article reviews
Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ccl21/Mouse+CCL21%2F6Ckine+Antibody/pmc12343296-253-28-34
Average 93 stars, based on 90 article reviews
ccl21 - by Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "The thrombin receptor PAR1 orchestrates changes in lymphatic endothelial cell junction morphology to augment lymphatic drainage during lung injury"
Article Title: The thrombin receptor PAR1 orchestrates changes in lymphatic endothelial cell junction morphology to augment lymphatic drainage during lung injury
Journal: Nature Cardiovascular Research
doi: 10.1038/s44161-025-00681-7
Figure Legend Snippet: ( a-d ) Tracing assay for VEGFR3Cre ERT2 using Lox-stop-lox ; TdTomato ; VEGFR3Cre ERT2 mice. ( a ) Merged image of Lox-stop-lox ; TdTomato ; VEGFR3Cre ERT2 mice after treatment with tamoxifen stained for anti-RFP (red, b ) and LEC marker anti-CCL21 (green, c ) demonstrating colocalization of TdTomato (red) and LECs (green) without significant TdTomato ( Cre reporter) expression outside of LECs. ( d ) Merged image of Lox-stop-lox ; TdTomato ; VEGFR3Cre ERT2 mice after treatment with tamoxifen stained for anti-RFP (red) and anti-VEGFR3 (LEC marker) also demonstrating colocalization of TdTomato (red) and LECs (green) without significant TdTomato expression outside of LECs. ( e ) Lung lymphatic endothelial cells (LECs) were isolated by flow cytometry for qPCR to assess knock-down efficiency of F2r (PAR1) in PAR1 iLECKO mice (n = 6) compared to PAR1 fl/fl mice (n = 3) with Gapd (GAPDH) as the reference housekeeping gene. Unpaired 2-tailed t- test; **, p = 0.0093. ( f ) BAL cell counts in room air PAR1 fl/fl (n = 4) and PAR1 iLEC KO (n = 4) mice compared to PAR1 fl/fl mice (n = 8) and PAR1 iLEC KO mice (n = 18) after 84 hours of hyperoxia exposure. One-way ANOVA; *, p = 0.0124; **, p = 0.0022; * p = 0.0332 from left to right. ( g, h ) Representative PCLS images demonstrating accumulation of CD45R + leukocytes (blue) in the peri-interstitial alveolar spaces adjacent to lymphatic collectors ( Prox1-EGFP , green) in PAR1 iLEC KO mice after hyperoxia-induced lung injury. BAL count and imaging data representative of at least 2 independent experiments. All error bars represent SEM. All scale bars = 200 µm.
Techniques Used: Staining, Marker, Expressing, Isolation, Flow Cytometry, Knockdown, Imaging
Related Articles
Blocking Assay:Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells. Article Snippet: In some experiments, DCs were added in the top well together with 20 nM murine plasminogen (Molecular Innovations) or with 8 nM murine plasmin (Molecular Innovations) chemotactic stimuli consisting of 100 ng/ml human CCL21 (PeproTech), 100 ng/ml human CCL21-ΔC (generated as described above), 100 ng/ml murine CXCL12 (PeproTech), 100 ng/ml murine CXCL12 and 20 nM murine plasminogen (Molecular Innovations), 100 ng/ml murine CXCL12, and 8 nM murine plasmin (Molecular Innovations). .. In some cases, 1 μg/ml of Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells. Article Snippet: .. Extracellular CCL21 staining in LN sections Inguinal LNs of adult (6–10 wk) WT and uPAmut mice were embedded in OCT. Unfixed, freshly cut 10-μm sections were blocked with 5% donkey serum (D9663; Sigma-Aldrich) in PBS and stained immediately with Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells Article Snippet: .. Inguinal LNs of adult (6–10 wk) WT and uPA mut mice were embedded in OCT. Unfixed, freshly cut 10-μm sections were blocked with 5% donkey serum (D9663; Sigma-Aldrich) in PBS and stained immediately with 96 Well Plates:Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells Article Snippet: .. Clear flat-bottom Immuno non-sterile 96-well plates (Thermo Fisher Scientific) were coated with Incubation:Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells Article Snippet: .. Clear flat-bottom Immuno non-sterile 96-well plates (Thermo Fisher Scientific) were coated with Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells. Article Snippet: .. Clear flat-bottom Immuno non-sterile 96-well plates (Thermo Fisher Scientific) were coated with other:Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells Article Snippet: In some experiments, DCs were added in the top well together with 20 nM murine plasminogen (Molecular Innovations) or with 8 nM murine plasmin (Molecular Innovations) chemotactic stimuli consisting of 100 ng/ml human CCL21 (PeproTech), 100 ng/ml human CCL21-ΔC (generated as described above), 100 ng/ml murine CXCL12 (PeproTech), 100 ng/ml murine CXCL12 and 20 nM murine plasminogen (Molecular Innovations), 100 ng/ml murine CXCL12, and 8 nM murine plasmin (Molecular Innovations). Control:Article Title: IFNγ-dependent metabolic reprogramming restrains an immature, pro-metastatic lymphatic state in melanoma. Article Snippet: Article Western Blot:Article Title: IFNγ-dependent metabolic reprogramming restrains an immature, pro-metastatic lymphatic state in melanoma. Article Snippet: Article Virus:Article Title: IFNγ-dependent metabolic reprogramming restrains an immature, pro-metastatic lymphatic state in melanoma. Article Snippet: Article Staining:Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells. Article Snippet: .. Extracellular CCL21 staining in LN sections Inguinal LNs of adult (6–10 wk) WT and uPAmut mice were embedded in OCT. Unfixed, freshly cut 10-μm sections were blocked with 5% donkey serum (D9663; Sigma-Aldrich) in PBS and stained immediately with Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells Article Snippet: .. Inguinal LNs of adult (6–10 wk) WT and uPA mut mice were embedded in OCT. Unfixed, freshly cut 10-μm sections were blocked with 5% donkey serum (D9663; Sigma-Aldrich) in PBS and stained immediately with |
