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mouse ccl21  (R&D Systems)


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    R&D Systems mouse ccl21
    Mouse Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/Recombinant+Mouse+CCL21%2F6Ckine+Protein/pm41917325-386-9-13
    Average 94 stars, based on 23 article reviews
    mouse ccl21 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: The critical role of Rap1-GAPs Rasa3 and Sipa1 in T cells for pulmonary transit and egress from the lymph nodes
    Article Snippet: CFSE (5- or 6-(N-succinimidyloxycarbonyl)-fluorescein 3′,6′-diacetate), CMTMR ((5-(and 6)-(((4-chloromethyl)benzoyl)amino) tetramethylrhodamine), and CMF2HC (4-chloromethyl-6,8-difluoro-7-hydroxycoumarin) dye for cell labeling were obtained from Invitrogen. .. Recombinant CCL21 was purchased from R&D Systems and S1P and FTY720 were obtained from Cayman Chemicals. .. Cells (1−2 × 10 6 ) were stained with fluorescent antibodies in culture medium (Iscove′s Modified Dulbecco′s medium supplemented with 2-mercaptoethanol and 4% fetal calf serum (4% FCS IMDM) and analyzed with an Attune NxT Flow Cytometer (Thermo Fisher) or a FACS Canto II flow cytometer (BD Bioscience).

    Article Title: The critical role of Rap1-GAPs Rasa3 and Sipa1 in T cells for pulmonary transit and egress from the lymph nodes.
    Article Snippet: CFSE (5- or 6-(N-succinimidyloxycarbonyl)-fluorescein 3′,6′-diacetate), CMTMR ((5-(and 6)-(((4-chloromethyl)benzoyl) amino) tetramethylrhodamine), and CMF2HC (4-chloromethyl6,8-difluoro-7-hydroxycoumarin) dye for cell labeling were obtained from Invitrogen. .. Recombinant CCL21 was purchased from R&D Systems and S1P and FTY720 were obtained from Cayman Chemicals. .. Cells (1−2 × 106) were stained with fluorescent antibodies in culture medium (Iscove′s Modified Dulbecco′s medium supplemented with 2-mercaptoethanol and 4% fetal calf serum (4% FCS IMDM) and analyzed with an Attune NxT Flow Cytometer (Thermo Fisher) or a FACS Canto II flow cytometer (BD Bioscience).

    Concentration Assay:

    Article Title: Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation
    Article Snippet: Cell migration assays were performed using an 8.0 μm pore polycarbonate membrane insert plate Transwell® (Corning, Life Science #3422). .. BMDCs were added to the upper chambers and the ligands CCL19 (R&D System #440-M3-025) and CCL21 (R&D System #457-6C-025) were added to the lower chambers at a 50 ng/μL concentration. ..

    other:

    Article Title: The TCR-SUB1-DOCK2 axis promotes autoimmunity by driving pathogenic CD4 + T cell tissue infiltration.
    Article Snippet: Article The TCR-SUB1-DOCK2 axis promotes autoimmunity by driving pathogenic CD4+ T cell tissue infiltration Highlights • SUB1 dictates the tissue infiltration of pathogenic CD4+ T cells • Sub1 expression is directly modulated by the TCR-IRF4 pathway • Sub1 deficiency prevents EAE onset by halving DOCK2 levels • SUB1 undergoes LLPS to open chromatin and drive the JUNB-DOCK2 transcriptional cascade Authors Xiaoxue Li, Wenhua Liang, Weifang Wang, Eilon Sherman, Keling Huang, Feng Wang Correspondence wangfeng16@sjtu.edu.cn In brief Blocking pathogenic T cell migration is crucial for treating autoimmune diseases.. Li et al. reveal SUB1 as a gatekeeper of CD4+ T cell infiltration in autoimmunity.. TCR-induced SUB1 drives Rac- dependent cytoskeletal remodeling via DOCK2 transcriptional activation.

    Sterility:




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    A Experimental design. Primary human CD8⁺ T cells were co-engineered with a lentiviral construct encoding the NR4A2-responsive synthetic promoter SP73 driving mCherry and constitutive EGFP to identify transduced cells, together with a retroviral NY-ESO-1–specific TCR. Engineered T cells were expanded and co-cultured with NY-ESO-1–expressing SK-MEL23 melanoma cells. B Quantification of mCherry⁺ T cells over time during co-culture. SP73-engineered T cells show progressive reporter induction, whereas control cells transduced with the control promoter SP01 (lacking NR4A2 binding sites) remain at baseline levels. Data represent mean ± SEM of n = 3 independent donors. C Representative IncuCyte® live-cell images showing SP73-driven mCherry fluorescence during co-culture with SK-MEL23 melanoma cells; SP01 controls show negligible signal. D Expression of NR4A2 and activation/dysfunction-associated markers in engineered T cells during co-culture. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. P values: CD39, P < 0.0001 for R0vsR2 and R0vsR4, P = 0.0048 for R2vsR4. NR4A2, P = 0.0375 for R0vsR2. LAG-3, P = 0.0194 for R0vsR2, P = 0.0097 for R0vsR4. TIM-3, P = 0.008 for R0vsR2, P = 0.0433 for R2vsR4. TIGIT, P = 0.0082 for R0vsR4, P = 0.0433 for R2vsR4. E Functional assessment of engineered T cells prior co-culture (R0) and following persistent antigen exposure (R4). Left: Quantification of IL-2⁺, TNFα⁺, CD107a⁺, and IFNγ⁺ cells following Leukocyte Activation Cocktail (LAC) stimulation. Right: Representative flow cytometry histograms illustrating shifts in cytokine-producing populations. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using a two-tailed paired t test comparing R0 vs R4. P values: IL-2, P = 0.0074; TNFα, P = 0.0068; CD107a, P = 0.0401; IFNγ, P = 0.0147. Source data for this figure is available in the file. Created in BioRender.

    Journal: Nature Communications

    Article Title: A modular synthetic promoter toolkit for programmable gene control in activated and chronically stimulated T cells

    doi: 10.1038/s41467-026-77730-7

    Figure Lengend Snippet: A Experimental design. Primary human CD8⁺ T cells were co-engineered with a lentiviral construct encoding the NR4A2-responsive synthetic promoter SP73 driving mCherry and constitutive EGFP to identify transduced cells, together with a retroviral NY-ESO-1–specific TCR. Engineered T cells were expanded and co-cultured with NY-ESO-1–expressing SK-MEL23 melanoma cells. B Quantification of mCherry⁺ T cells over time during co-culture. SP73-engineered T cells show progressive reporter induction, whereas control cells transduced with the control promoter SP01 (lacking NR4A2 binding sites) remain at baseline levels. Data represent mean ± SEM of n = 3 independent donors. C Representative IncuCyte® live-cell images showing SP73-driven mCherry fluorescence during co-culture with SK-MEL23 melanoma cells; SP01 controls show negligible signal. D Expression of NR4A2 and activation/dysfunction-associated markers in engineered T cells during co-culture. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. P values: CD39, P < 0.0001 for R0vsR2 and R0vsR4, P = 0.0048 for R2vsR4. NR4A2, P = 0.0375 for R0vsR2. LAG-3, P = 0.0194 for R0vsR2, P = 0.0097 for R0vsR4. TIM-3, P = 0.008 for R0vsR2, P = 0.0433 for R2vsR4. TIGIT, P = 0.0082 for R0vsR4, P = 0.0433 for R2vsR4. E Functional assessment of engineered T cells prior co-culture (R0) and following persistent antigen exposure (R4). Left: Quantification of IL-2⁺, TNFα⁺, CD107a⁺, and IFNγ⁺ cells following Leukocyte Activation Cocktail (LAC) stimulation. Right: Representative flow cytometry histograms illustrating shifts in cytokine-producing populations. Data represent mean ± SEM of n = 3 independent biological donors. Statistical analysis was performed using a two-tailed paired t test comparing R0 vs R4. P values: IL-2, P = 0.0074; TNFα, P = 0.0068; CD107a, P = 0.0401; IFNγ, P = 0.0147. Source data for this figure is available in the file. Created in BioRender.

    Article Snippet: The sequence of IL12p70 was obtained from pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe) (Addgene #105201 ).

    Techniques: Construct, Retroviral, Cell Culture, Expressing, Co-culture Assay, Control, Transduction, Binding Assay, Fluorescence, Activation Assay, Analysis, Functional Assay, Flow Cytometry, Two Tailed Test