Review



human ccl21  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    R&D Systems human ccl21
    Human Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/Recombinant+Human+CCL21%2F6Ckine+Protein/pm32665262-206-13-22
    Average 94 stars, based on 16 article reviews
    human ccl21 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: EPIGENETIC CONTROL OF NEURAL CREST DEVELOPMENT AND NEURAL CREST DERIVED TUMORS
    Article Snippet: For ciliation and Hedgehog signaling assays, cultures were transitioned to OptiMEM (Thermo Fisher Scientific, #31985062) and treated with recombinant Sonic Hedgehog 1 μg/ml (1845, R&D Systems, Minneapolis, MN) or vehicle control for 24 h. Subconfluent HSC cultures in 96 well plates were treated with vismodegib (Genentech) for 72 h, and cell proliferation was assayed on a GloMax Discovery plate reader (Promega, #PAGM3000) using the CellTiter 96 NonRadioactive Cell Proliferation kit (Promega, #G4100).

    Concentration Assay:

    Article Title: RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
    Article Snippet: .. Then medium with final FBS concentration 10% and 100 ng/ml CXCL12 (350- NS, R&D Systems) or 100 ng/ ml CCL21 (366- 6C , R&D Systems) was added to the wells. .. After 24 hr, cells were PFA fixed, permeabilized, and stained with Hoechst 33342 (H1399, Thermo Fisher Scientific) and Alexa Fluor 594 phalloidin.

    Blocking Assay:

    Article Title: Pyrrolopyrimidine and pyrrolopyridine derivatives
    Article Snippet: .. RPMI 1640 (Lonza Cat. No. BE12-115F/U1) Dimethyl sulfoxide (DMSO) (Sigma, Cat. No. D2650) Lipopolysaccharide from E. coli (LPS) (Sigma, Cat. No. L4391) Microplate, 96 well, pp, v-bottom, clear (Greiner; Cat. No. 651201) Master block 96 well, 2 ml (Greiner, Cat. No. 780271) Microplate, 96 well, ps, u-bottom, clear, with lid (Greiner, Cat. No. 650180) 50 mL falcon tube (TPP, Cat. No. 91050) Immulon 2HB 96-well plate (Thermo Fisher Scientific, Cat. No. 3455) Sucrose (Kemika, Cat. No. 1800408) Streptavidin-HRP (Calbiochem, Cat. No. OR03L) Sulphuric acid (Kemika, Cat. No. 1816501) anti-hCCL2 antibody (R&D Systems, Cat. No. MAB679), dissolved in 1 ml of PBS anti-hCCL2 detection antibody (R&D Systems, Cat. No. BAF279), dissolved in 1 ml of PBS Recombinant hCCL2 (standard, R&D Systems, Cat. No. 366-6C) Wash buffer (PBS+0.05% Tween-20) PBS (Sigma, Cat. No. P4417) Tween-20 (Sigma, Cat. No. P2287) Substrate for 20 ml: 18 ml H2O+2 ml 1M sodium acetate+200 μl TMB mix+2.5 μl 30% H2O2 Sodium acetate (Kemika, Cat. No. 1441908) TMB mix (Sigma, Cat. No. T2885) Hydrogen peroxide (Merck, Cat. No. 1.08597) Coating buffer (15 mM Na2CO3 (×H2O)+35 mM NaHCO3) Sodium carbonate (Sigma, Cat. No. S-2127) Sodium bicarbonate (Kemika, Cat. No. 1411007) Assay buffer (PBS+0.05% Tween-20+1% BSA) Bovine Serum Albumin (BSA) (Sigma, Cat. No. A2153) ..

    Recombinant:

    Article Title: Pyrrolopyrimidine and pyrrolopyridine derivatives
    Article Snippet: .. RPMI 1640 (Lonza Cat. No. BE12-115F/U1) Dimethyl sulfoxide (DMSO) (Sigma, Cat. No. D2650) Lipopolysaccharide from E. coli (LPS) (Sigma, Cat. No. L4391) Microplate, 96 well, pp, v-bottom, clear (Greiner; Cat. No. 651201) Master block 96 well, 2 ml (Greiner, Cat. No. 780271) Microplate, 96 well, ps, u-bottom, clear, with lid (Greiner, Cat. No. 650180) 50 mL falcon tube (TPP, Cat. No. 91050) Immulon 2HB 96-well plate (Thermo Fisher Scientific, Cat. No. 3455) Sucrose (Kemika, Cat. No. 1800408) Streptavidin-HRP (Calbiochem, Cat. No. OR03L) Sulphuric acid (Kemika, Cat. No. 1816501) anti-hCCL2 antibody (R&D Systems, Cat. No. MAB679), dissolved in 1 ml of PBS anti-hCCL2 detection antibody (R&D Systems, Cat. No. BAF279), dissolved in 1 ml of PBS Recombinant hCCL2 (standard, R&D Systems, Cat. No. 366-6C) Wash buffer (PBS+0.05% Tween-20) PBS (Sigma, Cat. No. P4417) Tween-20 (Sigma, Cat. No. P2287) Substrate for 20 ml: 18 ml H2O+2 ml 1M sodium acetate+200 μl TMB mix+2.5 μl 30% H2O2 Sodium acetate (Kemika, Cat. No. 1441908) TMB mix (Sigma, Cat. No. T2885) Hydrogen peroxide (Merck, Cat. No. 1.08597) Coating buffer (15 mM Na2CO3 (×H2O)+35 mM NaHCO3) Sodium carbonate (Sigma, Cat. No. S-2127) Sodium bicarbonate (Kemika, Cat. No. 1411007) Assay buffer (PBS+0.05% Tween-20+1% BSA) Bovine Serum Albumin (BSA) (Sigma, Cat. No. A2153) ..

    Article Title: Epigenetic reprogramming shapes the cellular landscape of schwannoma
    Article Snippet: The QCM Leukocyte Migration Assay (MilliporeSigma) and a GloMax Discovery plate reader (Promega) were used to quantify transwell T cell lymphocyte migration from the apical chamber over 4 hours at 37°C. .. Unconditioned HEI-193 media as a negative control, unconditioned HEI-193 media supplemented with recombinant human CCL21 600 ng/ml (366-6C, R&D Systems) as a positive control, HEI-193 media 5 days after 12.5Gy in 1 fraction, or HEI-193 media without radiation were placed in the basolateral chamber. ..

    Article Title: Immune regulation and lymphangiogenesis by lymphatic endothelial cells in the decidua in severe preeclampsia
    Article Snippet: The lower surface of the upper chamber was coated with 10 mg/mL fibronectin (Sigma-Aldrich, St Louis, MO, USA), and human DCs (1 × 10 5 ) were seeded onto the chemotaxis filters in EBM with 0.5% FBS. .. Conditioned media from PE or normal pregnancies collected after 24 h of culture in EBM with 1% FBS were added to the lower chamber, with or without recombinant human CCL21 (250 ng/mL; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Epigenetic reprogramming shapes the cellular landscape of schwannoma
    Article Snippet: The QCM Leukocyte Migration Assay (MilliporeSigma, # ECM557) and a GloMax Discovery plate reader (Promega, #PAGM3000) were used to quantify transwell T cell lymphocyte migration from the apical chamber over 4 h at 37 °C. .. Unconditioned HEI-193 media served as a negative control, unconditioned HEI-193 media supplemented with recombinant human CCL21 600 ng/ml (366-6C, R&D Systems) served as a positive control, HEI-193 media 5 days after 12.5 Gy in 1 fraction, or HEI-193 media without radiation were placed in the basolateral chamber. ..

    Negative Control:

    Article Title: Epigenetic reprogramming shapes the cellular landscape of schwannoma
    Article Snippet: The QCM Leukocyte Migration Assay (MilliporeSigma) and a GloMax Discovery plate reader (Promega) were used to quantify transwell T cell lymphocyte migration from the apical chamber over 4 hours at 37°C. .. Unconditioned HEI-193 media as a negative control, unconditioned HEI-193 media supplemented with recombinant human CCL21 600 ng/ml (366-6C, R&D Systems) as a positive control, HEI-193 media 5 days after 12.5Gy in 1 fraction, or HEI-193 media without radiation were placed in the basolateral chamber. ..

    Article Title: Epigenetic reprogramming shapes the cellular landscape of schwannoma
    Article Snippet: The QCM Leukocyte Migration Assay (MilliporeSigma, # ECM557) and a GloMax Discovery plate reader (Promega, #PAGM3000) were used to quantify transwell T cell lymphocyte migration from the apical chamber over 4 h at 37 °C. .. Unconditioned HEI-193 media served as a negative control, unconditioned HEI-193 media supplemented with recombinant human CCL21 600 ng/ml (366-6C, R&D Systems) served as a positive control, HEI-193 media 5 days after 12.5 Gy in 1 fraction, or HEI-193 media without radiation were placed in the basolateral chamber. ..

    Positive Control:

    Article Title: Epigenetic reprogramming shapes the cellular landscape of schwannoma
    Article Snippet: The QCM Leukocyte Migration Assay (MilliporeSigma) and a GloMax Discovery plate reader (Promega) were used to quantify transwell T cell lymphocyte migration from the apical chamber over 4 hours at 37°C. .. Unconditioned HEI-193 media as a negative control, unconditioned HEI-193 media supplemented with recombinant human CCL21 600 ng/ml (366-6C, R&D Systems) as a positive control, HEI-193 media 5 days after 12.5Gy in 1 fraction, or HEI-193 media without radiation were placed in the basolateral chamber. ..

    Article Title: Epigenetic reprogramming shapes the cellular landscape of schwannoma
    Article Snippet: The QCM Leukocyte Migration Assay (MilliporeSigma, # ECM557) and a GloMax Discovery plate reader (Promega, #PAGM3000) were used to quantify transwell T cell lymphocyte migration from the apical chamber over 4 h at 37 °C. .. Unconditioned HEI-193 media served as a negative control, unconditioned HEI-193 media supplemented with recombinant human CCL21 600 ng/ml (366-6C, R&D Systems) served as a positive control, HEI-193 media 5 days after 12.5 Gy in 1 fraction, or HEI-193 media without radiation were placed in the basolateral chamber. ..



    Similar Products

    98
    ABclonal Biotechnology ecl substrate
    Ecl Substrate, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pmc13563566-392-21-25
    Average 98 stars, based on 1 article reviews
    ecl substrate - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    93
    R&D Systems ccl21
    Ccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/Human+CCL21%2F6Ckine+DuoSet+ELISA/pm42775509-140-9-24
    Average 93 stars, based on 1 article reviews
    ccl21 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology ecl super kit
    Ecl Super Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pmc13587703-71-12-16
    Average 98 stars, based on 1 article reviews
    ecl super kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology ecl chemiluminescent substrate kit
    Ecl Chemiluminescent Substrate Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pmc13597843-61-5-9
    Average 98 stars, based on 1 article reviews
    ecl chemiluminescent substrate kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology ecl basic kit
    Ecl Basic Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pm42687121-71-1-7
    Average 98 stars, based on 1 article reviews
    ecl basic kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology enhanced chemiluminescence ecl substrate
    Enhanced Chemiluminescence Ecl Substrate, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pm42725518-125-12-18
    Average 98 stars, based on 1 article reviews
    enhanced chemiluminescence ecl substrate - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology ecl enhanced plus kit
    Ecl Enhanced Plus Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pmc13591283-261-7-11
    Average 98 stars, based on 1 article reviews
    ecl enhanced plus kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology chemiluminescence ecl
    Chemiluminescence Ecl, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pmc13425715-48-1-11
    Average 98 stars, based on 1 article reviews
    chemiluminescence ecl - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology enhanced chemiluminescence ecl kit
    Enhanced Chemiluminescence Ecl Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/CCL21+Rabbit+pAb/pmc13528181-319-5-9
    Average 98 stars, based on 1 article reviews
    enhanced chemiluminescence ecl kit - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    Image Search Results