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mab 610 human tnf mouse r d systems pc3 188a human cd3 mouse dako af366 human ccl21 goat r d systems 1f8 human cd21 mouse dako l  (R&D Systems)


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    Structured Review

    R&D Systems mab 610 human tnf mouse r d systems pc3 188a human cd3 mouse dako af366 human ccl21 goat r d systems 1f8 human cd21 mouse dako l
    Mab 610 Human Tnf Mouse R D Systems Pc3 188a Human Cd3 Mouse Dako Af366 Human Ccl21 Goat R D Systems 1f8 Human Cd21 Mouse Dako L, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl21/Human+CCL21%2F6Ckine+Antibody/pm11970972-213-79-84
    Average 93 stars, based on 49 article reviews
    mab 610 human tnf mouse r d systems pc3 188a human cd3 mouse dako af366 human ccl21 goat r d systems 1f8 human cd21 mouse dako l - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts.
    Article Snippet: .. Antibodies against human SERPINB9 (ThermoFisher, MA5-17648), CCL21 (R&D, AF366-SP) and MFGE8 (R&D, IC27671A) were used to verify transgene expression in hESCs and detected with secondary antibodies against mouse IgG (a488; ThermoFisher, A10680) or goat IgG (a488; ThermoFisher, A27012). .. Cell suspensions from hESC-derived teratomas or in vitro co-cultures were stained with antibodies against human CD45 (APC; BD Biosciences, 560973) and CD3 (Pe-Cy5; BD Biosciences, 561006).

    Incubation:

    Article Title: Pulmonary hypertension biomarker
    Article Snippet: Briefly, sections were dewaxed using EZprep solution, and high pH8 antigen retrieval was performed using the Ventana cc1 reagent. .. CCL21 was detected using goat anti-human CCL21 antibody (R&D System® AF366, 3.33 ug/ml)—the antibody was incubated for 12 hours at room temperature. .. Secondary antibody was biotinylated rabbit anti-goat (DAKO E0466) diluted to 1/200, 20 minute incubation at 37° C. Biotinylated secondary antibody was detected using the DABMap kit. (Ventana 650-010).

    Recombinant:

    Article Title: Chemokine binding to PSGL-1 is controlled by O-glycosylation and tyrosine sulfation.
    Article Snippet: In brief Using a chemoenzymatically synthesized library of PSGL-1 N-terminus glycosulfopeptides, Goth et al. identified key roles of these posttranslational modifications on interactions between PSGL-1 and chemokines.. Using microarray, fluorescence polarization, and chemotaxis assays, they discovered that tyrosine sulfation provides favorable interactions, while complexity of O-glycans negatively affects these interactions.

    Chromatin Immunoprecipitation:

    Article Title: Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary M arch 2021 Antibodies Antibodies used MPO : Rabbit polyclonal (ab9535) from Abcam CD8a : C8/144B clone from Abcam (ab17147) and clone RPA-T8 from Biolegend (301002) SMA : 1A4 clone from Abcam (ab7817) CCR7 : EPR23192-57 from Abcam (ab253187) HLA-DR : TAL 1B5 clone from Abcam (ab20181) ICOS : D1K2T clone from Cell Signaling (#89601) XBP1 : polyclonal from ThermoFisher (# PA5-27650) Histone H3K9Ac : C5B11 clone from Cell Signaling (#9649) HIF-1a : EP1215Y clone from Abcam (ab51608) CD20 : L26 clone from Abcam (ab9475) CD163 : EDHu-1 clone from Thermofisher (MA1-82342) CD56 : E7X9M clone from Cell Signaling (#99746) Granzyme B : D6E9W clone from Cell Signaling (# 46890) Human IgM : MHM-88 clone from Biolegend (314502) LEF1 : EPR2029Y clone from Abcam (ab137872) CD69 : EPR21814 clone from Abcam (ab233396) MMP9 : D6O3H clone from Cell Signaling (#13667) CD40L : EP462E clone from Abcam (ab210743) PD-L1 : 73-10 clone from Abcam (ab226766) CD45RO : UCHL1 clone from Biolegend (304202) FOXP3 : 236A/E7 clone from Thermofisher (# 14-4777-82) CXCL13 : Goat polyclonal from R&D Systems (AF801) CD9 : D3H4P clone from Cell Signaling (#13403) CD3 : Rabbit polyclonal from Agilent (A0452) GITR : D5V7P clone from Cell Signaling (#10419) CD303 : Goat polyclonal from R&D Systems (AF1376) CD209 : C209/1781 clone from Thermofisher (#30835-MSM1-P1ABX) AICDA : EPR23436 clone from Abcam (ab269457) MX1 : D3W7I clone from Cell Signaling (#37849) CCL21 : Goat polyclonal from R&D Systems (AF366) CD31 : EPR3094 clone from Abcam (ab76533) Cleaved Caspase3 : C92-605 clone from BD Biosciences (559565) CCL19 : 54909 clone from Thermofisher (# MA5-23833) Caveolin-1 : D46G3 clone from Cell Signaling (#3267) Vimentin : EPR3776 clone from Abcam (ab193555) Ki67 : B56 clone from BD (556003) IDO : SP260 clone from Abcam (ab228468) TCF1/TCF7 : C63D9 clone from Cell Signaling (#2203) BTLA : EPR22224-271 clone from Abcam (ab230976) Validation MPO : validated on human B-cell lymphoma (IHC). ..

    Article Title: Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-mouse anti-mouse CD3ε (BD Biosciences 553058) anti-mouse CD28 (BD Biosciences 553295) anti-mouse PD-L1 (alexa488, Novus NBP1-43262AF488) anti-mouse CD47, (BV421, BD Biosciences 740055) anti-mouse CD200 (alexa647, BD Biosciences 565544) anti-mouse FASL (Novus NBP1-97519) anti-mouse H2-M3 (BD Biosciences 551769) anti-mouse SERPINB9 (HycultBiotech HP8035) anti-mouse CCL21 (R&D AF457) anti-mouse MFGE8 (Biolegend 518603) anti-mouse CD31 (Biolegend 102417) anti-mouse Neurofilament (Fisher Scientific MS359R7) anti-mouse SSEA1 (Sigma MAB4301) anti-mouse OCT4 (Santa Cruz sc-8628) anti-mouse Smooth Muscle Actin (Abcam ab5694) anti-mouse FOXA2 (Abcam ab108422) anti-mouse SOX17 (R&D AF1924) anti-mouse Troponin (cTNT, abcam, ab8295) anti-mouse H-2Kb (Biolegend 116511) anti-mouse H-2Kq (Biolegend 115106) Anti-human anti-human PDL1 (APC, Biolegend 329708) anti-human FALSG (PE, Biolegend 306406) anti-human CD200 (APC/Cy7, Biolegend 329220) anti-human CD47 (PerCP/Cy5, Biolegend 323109) anti-human SERPINB9 (ThermoFisher MA5-17648) anti-human CCL21 (R&D AF366-SP) anti-human MFGE8 (R&D IC27671A) anti-human CD45 (APC, BD Biosciences 560973) anti-human CD3 (Pe-Cy5, BD Biosciences 561006) anti-human HLA-A,B,C (APC, Biolegend 311409) anti-human CD31 (Pe-Cy7, Biolegend 303118) anti-human CD144 (PE, Biolegend 138009) anti-human CD16/CD32 Fc blocker (BD Biosciences 553141) anti-human Human TruStain FxC Fc blocker (Biolegend 422301) Secondaries and Others anti mouse IgG (alexa488, ThermoFisher A10680) anti goat IgG (alexa488 ThermoFisher A27012) anti-Rat Fc (alexa568 secondary antibody (ThermoFisher A21112). anti-hamster (Dylight 488 Biolegend 405503) anti-rat IgG (DyLight 549 Novus NBP1-72975) anti-mouse (alexa647 ThermoFisher 21244) Annexin V (BV421, Biolegend 640923) PI (Biolegend, 421301) Validation Antibody performance and validations are available from the vendor and/or manufacturer. ..

    Flow Cytometry:

    Article Title: Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary M arch 2021 Antibodies Antibodies used MPO : Rabbit polyclonal (ab9535) from Abcam CD8a : C8/144B clone from Abcam (ab17147) and clone RPA-T8 from Biolegend (301002) SMA : 1A4 clone from Abcam (ab7817) CCR7 : EPR23192-57 from Abcam (ab253187) HLA-DR : TAL 1B5 clone from Abcam (ab20181) ICOS : D1K2T clone from Cell Signaling (#89601) XBP1 : polyclonal from ThermoFisher (# PA5-27650) Histone H3K9Ac : C5B11 clone from Cell Signaling (#9649) HIF-1a : EP1215Y clone from Abcam (ab51608) CD20 : L26 clone from Abcam (ab9475) CD163 : EDHu-1 clone from Thermofisher (MA1-82342) CD56 : E7X9M clone from Cell Signaling (#99746) Granzyme B : D6E9W clone from Cell Signaling (# 46890) Human IgM : MHM-88 clone from Biolegend (314502) LEF1 : EPR2029Y clone from Abcam (ab137872) CD69 : EPR21814 clone from Abcam (ab233396) MMP9 : D6O3H clone from Cell Signaling (#13667) CD40L : EP462E clone from Abcam (ab210743) PD-L1 : 73-10 clone from Abcam (ab226766) CD45RO : UCHL1 clone from Biolegend (304202) FOXP3 : 236A/E7 clone from Thermofisher (# 14-4777-82) CXCL13 : Goat polyclonal from R&D Systems (AF801) CD9 : D3H4P clone from Cell Signaling (#13403) CD3 : Rabbit polyclonal from Agilent (A0452) GITR : D5V7P clone from Cell Signaling (#10419) CD303 : Goat polyclonal from R&D Systems (AF1376) CD209 : C209/1781 clone from Thermofisher (#30835-MSM1-P1ABX) AICDA : EPR23436 clone from Abcam (ab269457) MX1 : D3W7I clone from Cell Signaling (#37849) CCL21 : Goat polyclonal from R&D Systems (AF366) CD31 : EPR3094 clone from Abcam (ab76533) Cleaved Caspase3 : C92-605 clone from BD Biosciences (559565) CCL19 : 54909 clone from Thermofisher (# MA5-23833) Caveolin-1 : D46G3 clone from Cell Signaling (#3267) Vimentin : EPR3776 clone from Abcam (ab193555) Ki67 : B56 clone from BD (556003) IDO : SP260 clone from Abcam (ab228468) TCF1/TCF7 : C63D9 clone from Cell Signaling (#2203) BTLA : EPR22224-271 clone from Abcam (ab230976) Validation MPO : validated on human B-cell lymphoma (IHC). ..

    Article Title: Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-mouse anti-mouse CD3ε (BD Biosciences 553058) anti-mouse CD28 (BD Biosciences 553295) anti-mouse PD-L1 (alexa488, Novus NBP1-43262AF488) anti-mouse CD47, (BV421, BD Biosciences 740055) anti-mouse CD200 (alexa647, BD Biosciences 565544) anti-mouse FASL (Novus NBP1-97519) anti-mouse H2-M3 (BD Biosciences 551769) anti-mouse SERPINB9 (HycultBiotech HP8035) anti-mouse CCL21 (R&D AF457) anti-mouse MFGE8 (Biolegend 518603) anti-mouse CD31 (Biolegend 102417) anti-mouse Neurofilament (Fisher Scientific MS359R7) anti-mouse SSEA1 (Sigma MAB4301) anti-mouse OCT4 (Santa Cruz sc-8628) anti-mouse Smooth Muscle Actin (Abcam ab5694) anti-mouse FOXA2 (Abcam ab108422) anti-mouse SOX17 (R&D AF1924) anti-mouse Troponin (cTNT, abcam, ab8295) anti-mouse H-2Kb (Biolegend 116511) anti-mouse H-2Kq (Biolegend 115106) Anti-human anti-human PDL1 (APC, Biolegend 329708) anti-human FALSG (PE, Biolegend 306406) anti-human CD200 (APC/Cy7, Biolegend 329220) anti-human CD47 (PerCP/Cy5, Biolegend 323109) anti-human SERPINB9 (ThermoFisher MA5-17648) anti-human CCL21 (R&D AF366-SP) anti-human MFGE8 (R&D IC27671A) anti-human CD45 (APC, BD Biosciences 560973) anti-human CD3 (Pe-Cy5, BD Biosciences 561006) anti-human HLA-A,B,C (APC, Biolegend 311409) anti-human CD31 (Pe-Cy7, Biolegend 303118) anti-human CD144 (PE, Biolegend 138009) anti-human CD16/CD32 Fc blocker (BD Biosciences 553141) anti-human Human TruStain FxC Fc blocker (Biolegend 422301) Secondaries and Others anti mouse IgG (alexa488, ThermoFisher A10680) anti goat IgG (alexa488 ThermoFisher A27012) anti-Rat Fc (alexa568 secondary antibody (ThermoFisher A21112). anti-hamster (Dylight 488 Biolegend 405503) anti-rat IgG (DyLight 549 Novus NBP1-72975) anti-mouse (alexa647 ThermoFisher 21244) Annexin V (BV421, Biolegend 640923) PI (Biolegend, 421301) Validation Antibody performance and validations are available from the vendor and/or manufacturer. ..

    Magnetic Resonance Imaging:

    Article Title: Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary M arch 2021 Antibodies Antibodies used MPO : Rabbit polyclonal (ab9535) from Abcam CD8a : C8/144B clone from Abcam (ab17147) and clone RPA-T8 from Biolegend (301002) SMA : 1A4 clone from Abcam (ab7817) CCR7 : EPR23192-57 from Abcam (ab253187) HLA-DR : TAL 1B5 clone from Abcam (ab20181) ICOS : D1K2T clone from Cell Signaling (#89601) XBP1 : polyclonal from ThermoFisher (# PA5-27650) Histone H3K9Ac : C5B11 clone from Cell Signaling (#9649) HIF-1a : EP1215Y clone from Abcam (ab51608) CD20 : L26 clone from Abcam (ab9475) CD163 : EDHu-1 clone from Thermofisher (MA1-82342) CD56 : E7X9M clone from Cell Signaling (#99746) Granzyme B : D6E9W clone from Cell Signaling (# 46890) Human IgM : MHM-88 clone from Biolegend (314502) LEF1 : EPR2029Y clone from Abcam (ab137872) CD69 : EPR21814 clone from Abcam (ab233396) MMP9 : D6O3H clone from Cell Signaling (#13667) CD40L : EP462E clone from Abcam (ab210743) PD-L1 : 73-10 clone from Abcam (ab226766) CD45RO : UCHL1 clone from Biolegend (304202) FOXP3 : 236A/E7 clone from Thermofisher (# 14-4777-82) CXCL13 : Goat polyclonal from R&D Systems (AF801) CD9 : D3H4P clone from Cell Signaling (#13403) CD3 : Rabbit polyclonal from Agilent (A0452) GITR : D5V7P clone from Cell Signaling (#10419) CD303 : Goat polyclonal from R&D Systems (AF1376) CD209 : C209/1781 clone from Thermofisher (#30835-MSM1-P1ABX) AICDA : EPR23436 clone from Abcam (ab269457) MX1 : D3W7I clone from Cell Signaling (#37849) CCL21 : Goat polyclonal from R&D Systems (AF366) CD31 : EPR3094 clone from Abcam (ab76533) Cleaved Caspase3 : C92-605 clone from BD Biosciences (559565) CCL19 : 54909 clone from Thermofisher (# MA5-23833) Caveolin-1 : D46G3 clone from Cell Signaling (#3267) Vimentin : EPR3776 clone from Abcam (ab193555) Ki67 : B56 clone from BD (556003) IDO : SP260 clone from Abcam (ab228468) TCF1/TCF7 : C63D9 clone from Cell Signaling (#2203) BTLA : EPR22224-271 clone from Abcam (ab230976) Validation MPO : validated on human B-cell lymphoma (IHC). ..

    Article Title: Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-mouse anti-mouse CD3ε (BD Biosciences 553058) anti-mouse CD28 (BD Biosciences 553295) anti-mouse PD-L1 (alexa488, Novus NBP1-43262AF488) anti-mouse CD47, (BV421, BD Biosciences 740055) anti-mouse CD200 (alexa647, BD Biosciences 565544) anti-mouse FASL (Novus NBP1-97519) anti-mouse H2-M3 (BD Biosciences 551769) anti-mouse SERPINB9 (HycultBiotech HP8035) anti-mouse CCL21 (R&D AF457) anti-mouse MFGE8 (Biolegend 518603) anti-mouse CD31 (Biolegend 102417) anti-mouse Neurofilament (Fisher Scientific MS359R7) anti-mouse SSEA1 (Sigma MAB4301) anti-mouse OCT4 (Santa Cruz sc-8628) anti-mouse Smooth Muscle Actin (Abcam ab5694) anti-mouse FOXA2 (Abcam ab108422) anti-mouse SOX17 (R&D AF1924) anti-mouse Troponin (cTNT, abcam, ab8295) anti-mouse H-2Kb (Biolegend 116511) anti-mouse H-2Kq (Biolegend 115106) Anti-human anti-human PDL1 (APC, Biolegend 329708) anti-human FALSG (PE, Biolegend 306406) anti-human CD200 (APC/Cy7, Biolegend 329220) anti-human CD47 (PerCP/Cy5, Biolegend 323109) anti-human SERPINB9 (ThermoFisher MA5-17648) anti-human CCL21 (R&D AF366-SP) anti-human MFGE8 (R&D IC27671A) anti-human CD45 (APC, BD Biosciences 560973) anti-human CD3 (Pe-Cy5, BD Biosciences 561006) anti-human HLA-A,B,C (APC, Biolegend 311409) anti-human CD31 (Pe-Cy7, Biolegend 303118) anti-human CD144 (PE, Biolegend 138009) anti-human CD16/CD32 Fc blocker (BD Biosciences 553141) anti-human Human TruStain FxC Fc blocker (Biolegend 422301) Secondaries and Others anti mouse IgG (alexa488, ThermoFisher A10680) anti goat IgG (alexa488 ThermoFisher A27012) anti-Rat Fc (alexa568 secondary antibody (ThermoFisher A21112). anti-hamster (Dylight 488 Biolegend 405503) anti-rat IgG (DyLight 549 Novus NBP1-72975) anti-mouse (alexa647 ThermoFisher 21244) Annexin V (BV421, Biolegend 640923) PI (Biolegend, 421301) Validation Antibody performance and validations are available from the vendor and/or manufacturer. ..

    Recombinase Polymerase Amplification:

    Article Title: Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary M arch 2021 Antibodies Antibodies used MPO : Rabbit polyclonal (ab9535) from Abcam CD8a : C8/144B clone from Abcam (ab17147) and clone RPA-T8 from Biolegend (301002) SMA : 1A4 clone from Abcam (ab7817) CCR7 : EPR23192-57 from Abcam (ab253187) HLA-DR : TAL 1B5 clone from Abcam (ab20181) ICOS : D1K2T clone from Cell Signaling (#89601) XBP1 : polyclonal from ThermoFisher (# PA5-27650) Histone H3K9Ac : C5B11 clone from Cell Signaling (#9649) HIF-1a : EP1215Y clone from Abcam (ab51608) CD20 : L26 clone from Abcam (ab9475) CD163 : EDHu-1 clone from Thermofisher (MA1-82342) CD56 : E7X9M clone from Cell Signaling (#99746) Granzyme B : D6E9W clone from Cell Signaling (# 46890) Human IgM : MHM-88 clone from Biolegend (314502) LEF1 : EPR2029Y clone from Abcam (ab137872) CD69 : EPR21814 clone from Abcam (ab233396) MMP9 : D6O3H clone from Cell Signaling (#13667) CD40L : EP462E clone from Abcam (ab210743) PD-L1 : 73-10 clone from Abcam (ab226766) CD45RO : UCHL1 clone from Biolegend (304202) FOXP3 : 236A/E7 clone from Thermofisher (# 14-4777-82) CXCL13 : Goat polyclonal from R&D Systems (AF801) CD9 : D3H4P clone from Cell Signaling (#13403) CD3 : Rabbit polyclonal from Agilent (A0452) GITR : D5V7P clone from Cell Signaling (#10419) CD303 : Goat polyclonal from R&D Systems (AF1376) CD209 : C209/1781 clone from Thermofisher (#30835-MSM1-P1ABX) AICDA : EPR23436 clone from Abcam (ab269457) MX1 : D3W7I clone from Cell Signaling (#37849) CCL21 : Goat polyclonal from R&D Systems (AF366) CD31 : EPR3094 clone from Abcam (ab76533) Cleaved Caspase3 : C92-605 clone from BD Biosciences (559565) CCL19 : 54909 clone from Thermofisher (# MA5-23833) Caveolin-1 : D46G3 clone from Cell Signaling (#3267) Vimentin : EPR3776 clone from Abcam (ab193555) Ki67 : B56 clone from BD (556003) IDO : SP260 clone from Abcam (ab228468) TCF1/TCF7 : C63D9 clone from Cell Signaling (#2203) BTLA : EPR22224-271 clone from Abcam (ab230976) Validation MPO : validated on human B-cell lymphoma (IHC). ..

    Biomarker Discovery:

    Article Title: Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary M arch 2021 Antibodies Antibodies used MPO : Rabbit polyclonal (ab9535) from Abcam CD8a : C8/144B clone from Abcam (ab17147) and clone RPA-T8 from Biolegend (301002) SMA : 1A4 clone from Abcam (ab7817) CCR7 : EPR23192-57 from Abcam (ab253187) HLA-DR : TAL 1B5 clone from Abcam (ab20181) ICOS : D1K2T clone from Cell Signaling (#89601) XBP1 : polyclonal from ThermoFisher (# PA5-27650) Histone H3K9Ac : C5B11 clone from Cell Signaling (#9649) HIF-1a : EP1215Y clone from Abcam (ab51608) CD20 : L26 clone from Abcam (ab9475) CD163 : EDHu-1 clone from Thermofisher (MA1-82342) CD56 : E7X9M clone from Cell Signaling (#99746) Granzyme B : D6E9W clone from Cell Signaling (# 46890) Human IgM : MHM-88 clone from Biolegend (314502) LEF1 : EPR2029Y clone from Abcam (ab137872) CD69 : EPR21814 clone from Abcam (ab233396) MMP9 : D6O3H clone from Cell Signaling (#13667) CD40L : EP462E clone from Abcam (ab210743) PD-L1 : 73-10 clone from Abcam (ab226766) CD45RO : UCHL1 clone from Biolegend (304202) FOXP3 : 236A/E7 clone from Thermofisher (# 14-4777-82) CXCL13 : Goat polyclonal from R&D Systems (AF801) CD9 : D3H4P clone from Cell Signaling (#13403) CD3 : Rabbit polyclonal from Agilent (A0452) GITR : D5V7P clone from Cell Signaling (#10419) CD303 : Goat polyclonal from R&D Systems (AF1376) CD209 : C209/1781 clone from Thermofisher (#30835-MSM1-P1ABX) AICDA : EPR23436 clone from Abcam (ab269457) MX1 : D3W7I clone from Cell Signaling (#37849) CCL21 : Goat polyclonal from R&D Systems (AF366) CD31 : EPR3094 clone from Abcam (ab76533) Cleaved Caspase3 : C92-605 clone from BD Biosciences (559565) CCL19 : 54909 clone from Thermofisher (# MA5-23833) Caveolin-1 : D46G3 clone from Cell Signaling (#3267) Vimentin : EPR3776 clone from Abcam (ab193555) Ki67 : B56 clone from BD (556003) IDO : SP260 clone from Abcam (ab228468) TCF1/TCF7 : C63D9 clone from Cell Signaling (#2203) BTLA : EPR22224-271 clone from Abcam (ab230976) Validation MPO : validated on human B-cell lymphoma (IHC). ..

    Article Title: Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-mouse anti-mouse CD3ε (BD Biosciences 553058) anti-mouse CD28 (BD Biosciences 553295) anti-mouse PD-L1 (alexa488, Novus NBP1-43262AF488) anti-mouse CD47, (BV421, BD Biosciences 740055) anti-mouse CD200 (alexa647, BD Biosciences 565544) anti-mouse FASL (Novus NBP1-97519) anti-mouse H2-M3 (BD Biosciences 551769) anti-mouse SERPINB9 (HycultBiotech HP8035) anti-mouse CCL21 (R&D AF457) anti-mouse MFGE8 (Biolegend 518603) anti-mouse CD31 (Biolegend 102417) anti-mouse Neurofilament (Fisher Scientific MS359R7) anti-mouse SSEA1 (Sigma MAB4301) anti-mouse OCT4 (Santa Cruz sc-8628) anti-mouse Smooth Muscle Actin (Abcam ab5694) anti-mouse FOXA2 (Abcam ab108422) anti-mouse SOX17 (R&D AF1924) anti-mouse Troponin (cTNT, abcam, ab8295) anti-mouse H-2Kb (Biolegend 116511) anti-mouse H-2Kq (Biolegend 115106) Anti-human anti-human PDL1 (APC, Biolegend 329708) anti-human FALSG (PE, Biolegend 306406) anti-human CD200 (APC/Cy7, Biolegend 329220) anti-human CD47 (PerCP/Cy5, Biolegend 323109) anti-human SERPINB9 (ThermoFisher MA5-17648) anti-human CCL21 (R&D AF366-SP) anti-human MFGE8 (R&D IC27671A) anti-human CD45 (APC, BD Biosciences 560973) anti-human CD3 (Pe-Cy5, BD Biosciences 561006) anti-human HLA-A,B,C (APC, Biolegend 311409) anti-human CD31 (Pe-Cy7, Biolegend 303118) anti-human CD144 (PE, Biolegend 138009) anti-human CD16/CD32 Fc blocker (BD Biosciences 553141) anti-human Human TruStain FxC Fc blocker (Biolegend 422301) Secondaries and Others anti mouse IgG (alexa488, ThermoFisher A10680) anti goat IgG (alexa488 ThermoFisher A27012) anti-Rat Fc (alexa568 secondary antibody (ThermoFisher A21112). anti-hamster (Dylight 488 Biolegend 405503) anti-rat IgG (DyLight 549 Novus NBP1-72975) anti-mouse (alexa647 ThermoFisher 21244) Annexin V (BV421, Biolegend 640923) PI (Biolegend, 421301) Validation Antibody performance and validations are available from the vendor and/or manufacturer. ..

    Immunohistochemistry:

    Article Title: Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging ary M arch 2021 Antibodies Antibodies used MPO : Rabbit polyclonal (ab9535) from Abcam CD8a : C8/144B clone from Abcam (ab17147) and clone RPA-T8 from Biolegend (301002) SMA : 1A4 clone from Abcam (ab7817) CCR7 : EPR23192-57 from Abcam (ab253187) HLA-DR : TAL 1B5 clone from Abcam (ab20181) ICOS : D1K2T clone from Cell Signaling (#89601) XBP1 : polyclonal from ThermoFisher (# PA5-27650) Histone H3K9Ac : C5B11 clone from Cell Signaling (#9649) HIF-1a : EP1215Y clone from Abcam (ab51608) CD20 : L26 clone from Abcam (ab9475) CD163 : EDHu-1 clone from Thermofisher (MA1-82342) CD56 : E7X9M clone from Cell Signaling (#99746) Granzyme B : D6E9W clone from Cell Signaling (# 46890) Human IgM : MHM-88 clone from Biolegend (314502) LEF1 : EPR2029Y clone from Abcam (ab137872) CD69 : EPR21814 clone from Abcam (ab233396) MMP9 : D6O3H clone from Cell Signaling (#13667) CD40L : EP462E clone from Abcam (ab210743) PD-L1 : 73-10 clone from Abcam (ab226766) CD45RO : UCHL1 clone from Biolegend (304202) FOXP3 : 236A/E7 clone from Thermofisher (# 14-4777-82) CXCL13 : Goat polyclonal from R&D Systems (AF801) CD9 : D3H4P clone from Cell Signaling (#13403) CD3 : Rabbit polyclonal from Agilent (A0452) GITR : D5V7P clone from Cell Signaling (#10419) CD303 : Goat polyclonal from R&D Systems (AF1376) CD209 : C209/1781 clone from Thermofisher (#30835-MSM1-P1ABX) AICDA : EPR23436 clone from Abcam (ab269457) MX1 : D3W7I clone from Cell Signaling (#37849) CCL21 : Goat polyclonal from R&D Systems (AF366) CD31 : EPR3094 clone from Abcam (ab76533) Cleaved Caspase3 : C92-605 clone from BD Biosciences (559565) CCL19 : 54909 clone from Thermofisher (# MA5-23833) Caveolin-1 : D46G3 clone from Cell Signaling (#3267) Vimentin : EPR3776 clone from Abcam (ab193555) Ki67 : B56 clone from BD (556003) IDO : SP260 clone from Abcam (ab228468) TCF1/TCF7 : C63D9 clone from Cell Signaling (#2203) BTLA : EPR22224-271 clone from Abcam (ab230976) Validation MPO : validated on human B-cell lymphoma (IHC). ..



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