Review



assay catalog code thpd nfis  (InvivoGen)


Bioz Verified Symbol InvivoGen is a verified supplier
Bioz Manufacturer Symbol InvivoGen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    InvivoGen assay catalog code thpd nfis
    Assay Catalog Code Thpd Nfis, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/THP1-Dual+Cells/us12036231-660-7-4
    Average 98 stars, based on 424 article reviews
    assay catalog code thpd nfis - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: Adsorption-mediated modulation of lipopolysaccharide bioactivity by clinoptilolite zeolite with in vitro immunomodulatory effects and in vivo safety evaluation
    Article Snippet: .. Residual LPS activity in the supernatant was assessed indirectly by measuring NF-κB activation in THP-1 Dual cells using the QUANTI-BlueTM assay (InvivoGen), according to the manufacturer’s protocol. .. Residual LPS activity in the supernatant was assessed indirectly by measuring NF-κB activation in THP-1 Dual cells using the QUANTI-Blue assay (InvivoGen), according to the manufacturer’s protocol.

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: PBMCs were also pretreated with 5 μM CATPB (Medchem Express, Monmouth Junction, NJ, U.S.A., CAS No. 1322598-09-3) and Bifidobacterium fermentation supernatants for 2 h prior to stimulation. .. NF-κB activity was assessed in THP1-Dual Cells (InvivoGen, Toulouse, France), a monocyte-derived reporter cell line stably expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. .. SEAP activity was quantified in cell culture supernatants using QUANTI-Blue (InvivoGen, Toulouse, France) according to the manufacturer's instructions.

    Activation Assay:

    Article Title: Adsorption-mediated modulation of lipopolysaccharide bioactivity by clinoptilolite zeolite with in vitro immunomodulatory effects and in vivo safety evaluation
    Article Snippet: .. Residual LPS activity in the supernatant was assessed indirectly by measuring NF-κB activation in THP-1 Dual cells using the QUANTI-BlueTM assay (InvivoGen), according to the manufacturer’s protocol. .. Residual LPS activity in the supernatant was assessed indirectly by measuring NF-κB activation in THP-1 Dual cells using the QUANTI-Blue assay (InvivoGen), according to the manufacturer’s protocol.

    Stable Transfection:

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: PBMCs were also pretreated with 5 μM CATPB (Medchem Express, Monmouth Junction, NJ, U.S.A., CAS No. 1322598-09-3) and Bifidobacterium fermentation supernatants for 2 h prior to stimulation. .. NF-κB activity was assessed in THP1-Dual Cells (InvivoGen, Toulouse, France), a monocyte-derived reporter cell line stably expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. .. SEAP activity was quantified in cell culture supernatants using QUANTI-Blue (InvivoGen, Toulouse, France) according to the manufacturer's instructions.

    Expressing:

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: PBMCs were also pretreated with 5 μM CATPB (Medchem Express, Monmouth Junction, NJ, U.S.A., CAS No. 1322598-09-3) and Bifidobacterium fermentation supernatants for 2 h prior to stimulation. .. NF-κB activity was assessed in THP1-Dual Cells (InvivoGen, Toulouse, France), a monocyte-derived reporter cell line stably expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. .. SEAP activity was quantified in cell culture supernatants using QUANTI-Blue (InvivoGen, Toulouse, France) according to the manufacturer's instructions.

    Cell Culture:

    Article Title: Herring roe oil exerts immunomodulatory effects on the IL-17/23 signaling axis in immune and skin cells as models for psoriasis
    Article Snippet: .. THP1-Dual NF-κB-SEAP IRF-Luc Reporter monocytes (InvivoGen, thpd-nfis) were cultured in RPMI 1640 (Thermofisher, 61870036) with 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, F0804), 100 U/mL penicillin + 0.1 mg/mL streptomycin (Sigma-Aldrich, P4333). .. The cells were differentiated to MDMs by seeding with 40 ng/mL Phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P8139) and incubation for 3 d, followed by 1.5 d in fresh medium without PMA.

    Article Title: IPO9 modulates histone-dependent inhibition of cGAS activity
    Article Snippet: .. THP-1 Dual (thpd-nfis) and THP-1 Lucia ISG (thpl-isg) cells were purchased from Invivogen and cultured in media consisting of RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% fetal bovine serum, and 1,000 units/mL penicillin, 1,000 μg/mL streptomycin, and 0.25 μg/mL Amphotericin B. .. THP-1 Dual (thpd-nfis) and THP-1 Lucia ISG (thpl-isg) cells were purchased from Invivogen and cultured in media consisting of RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% fetal bovine serum, and 1,000 units/mL penicillin, 1,000 μg/mL streptomycin, and 0.25 μg/mL Amphotericin B.

    Article Title: Tuning intracellular immunity by Nodamura virus B2 protein enhances self-amplifying RNA activity
    Article Snippet: HeLa (ATCC CCL-2), HeLa PKR −/−, HEK293 (ATCC CRL-1573), and HEK293T (ATCC CRL-3216) and BHK-21 (ATCC CCL-10) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; 31966, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, FB-1001, Biosera) and 100 U ml −1 penicillin/streptomycin (15140, Gibco) at 37 °C in 5% CO2. .. THP-1-Dual (thpd-nfis, InvivoGen) and THP-1-Dual KO-IFNAR2 cells (InvivoGen) were cultured in Roswell Park Memorial Institute 1640 Medium (RPMI, 21875, Gibco) containing 10% FBS, and100 U ml −1 penicillin/streptomycin. .. E14 mESCs (CRL-1821) were cultured in DMEM supplemented with 10% embryonic stem cell qualified fetal calf serum (FCS, Gibco), 2 mM non-essential amino acids (25-025-CIR, Gibco), 2 mM sodium pyruvate (S8636, Sigma-Aldrich), 2mM L-glutamine (25030, Gibco), 100 U ml −1 penicillin/streptomycin, 0.2% β-mercaptoethanol (31350, Gibco), and 1000 U ml −1 ESGRO recombinant mouse leukaemia inhibitory factor (ESG1107, Sigma-Aldrich), at 37 °C with 5% CO2 in gelatine-coated plates.

    Gene Knockout:

    Article Title: Allosteric capsid inhibitors and their escape mutants drive HIV-1 sensing
    Article Snippet: .. THP-1 Dual cells (cGAS KO and NT control, Invivogen) express Lucia luciferase as a marker of the IRF pathway and were additionally supplemented with 12.5 mM HEPES (Sigma), 10 μg/mL of blasticidin (Invivogen) and 100 μg/mL of zeocin (Invivogen). .. Peripheral blood mononuclear cells (PBMC) were isolated from leukocyte cones from healthy donors (UK NHS Blood and Transplant Service) by density centrifugation using FicollPaque Plus (GE Life Sciences).

    Article Title: Tuning intracellular immunity by Nodamura virus B2 protein enhances self-amplifying RNA activity
    Article Snippet: HeLa (ATCC CCL-2), HeLa PKR −/−, HEK293 (ATCC CRL-1573), and HEK293T (ATCC CRL-3216) and BHK-21 (ATCC CCL-10) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; 31966, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, FB-1001, Biosera) and 100 U ml −1 penicillin/streptomycin (15140, Gibco) at 37 °C in 5% CO2. .. THP-1-Dual (thpd-nfis, InvivoGen) and THP-1-Dual KO-IFNAR2 cells (InvivoGen) were cultured in Roswell Park Memorial Institute 1640 Medium (RPMI, 21875, Gibco) containing 10% FBS, and100 U ml −1 penicillin/streptomycin. .. E14 mESCs (CRL-1821) were cultured in DMEM supplemented with 10% embryonic stem cell qualified fetal calf serum (FCS, Gibco), 2 mM non-essential amino acids (25-025-CIR, Gibco), 2 mM sodium pyruvate (S8636, Sigma-Aldrich), 2mM L-glutamine (25030, Gibco), 100 U ml −1 penicillin/streptomycin, 0.2% β-mercaptoethanol (31350, Gibco), and 1000 U ml −1 ESGRO recombinant mouse leukaemia inhibitory factor (ESG1107, Sigma-Aldrich), at 37 °C with 5% CO2 in gelatine-coated plates.

    Control:

    Article Title: Allosteric capsid inhibitors and their escape mutants drive HIV-1 sensing
    Article Snippet: .. THP-1 Dual cells (cGAS KO and NT control, Invivogen) express Lucia luciferase as a marker of the IRF pathway and were additionally supplemented with 12.5 mM HEPES (Sigma), 10 μg/mL of blasticidin (Invivogen) and 100 μg/mL of zeocin (Invivogen). .. Peripheral blood mononuclear cells (PBMC) were isolated from leukocyte cones from healthy donors (UK NHS Blood and Transplant Service) by density centrifugation using FicollPaque Plus (GE Life Sciences).

    Luciferase:

    Article Title: Allosteric capsid inhibitors and their escape mutants drive HIV-1 sensing
    Article Snippet: .. THP-1 Dual cells (cGAS KO and NT control, Invivogen) express Lucia luciferase as a marker of the IRF pathway and were additionally supplemented with 12.5 mM HEPES (Sigma), 10 μg/mL of blasticidin (Invivogen) and 100 μg/mL of zeocin (Invivogen). .. Peripheral blood mononuclear cells (PBMC) were isolated from leukocyte cones from healthy donors (UK NHS Blood and Transplant Service) by density centrifugation using FicollPaque Plus (GE Life Sciences).

    Marker:

    Article Title: Allosteric capsid inhibitors and their escape mutants drive HIV-1 sensing
    Article Snippet: .. THP-1 Dual cells (cGAS KO and NT control, Invivogen) express Lucia luciferase as a marker of the IRF pathway and were additionally supplemented with 12.5 mM HEPES (Sigma), 10 μg/mL of blasticidin (Invivogen) and 100 μg/mL of zeocin (Invivogen). .. Peripheral blood mononuclear cells (PBMC) were isolated from leukocyte cones from healthy donors (UK NHS Blood and Transplant Service) by density centrifugation using FicollPaque Plus (GE Life Sciences).

    other:

    Article Title: The genomic sequence of packaged viral ribonucleic acid predicts mucosal HIV-1 transmission fitness
    Article Snippet: THP-1 Dual reporter cells , InvivoGen - thpd-nfis RRID:CVCL_X599 , THP-1 Dual.

    Reporter Assay:

    Article Title: MAP Crosslinker Chirality Regulates Macrophage Polarization Through MD-2 Engagement and Enables Tunable Immunomodulation Through Novel Peptide Design.
    Article Snippet: Microporous annealed particle (MAP) scaffolds are injectable hydrogel biomaterials that promote tissue regeneration by enabling rapid cell infiltration and presenting reparative cues.. Previous work showed that substituting Lwith D-chiral residues in matrix metalloproteinase (MMP)-degradable crosslinkers induces adaptive immune-mediated skin regeneration and balanced macrophage responses in vivo, but the underlying mechanisms remain unclear.. Here, we identify myeloid differentiation factor 2 (MD-2)-associated TLR4 signaling as a key mechanistic contributor to macrophage polarization driven by crosslinker chirality in MAP scaffolds.



    Similar Products

    e coli k12 invivogen catalog code tlrl kipgn  (InvivoGen)
    94
    InvivoGen e coli k12 invivogen catalog code tlrl kipgn
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    E Coli K12 Invivogen Catalog Code Tlrl Kipgn, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/PGN-ECndi+ultrapure%2C+insoluble/pmc13657326-257-2-5
    Average 94 stars, based on 1 article reviews
    e coli k12 invivogen catalog code tlrl kipgn - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    pgn ecndi ultrapure peptidoglycan catalog code  (InvivoGen)
    94
    InvivoGen pgn ecndi ultrapure peptidoglycan catalog code
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Pgn Ecndi Ultrapure Peptidoglycan Catalog Code, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/PGN-ECndi+ultrapure%2C+insoluble/bio_rxiv__64898__2025__12__22__695905-286-0-6
    Average 94 stars, based on 1 article reviews
    pgn ecndi ultrapure peptidoglycan catalog code - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    catalog code ant pr 1  (InvivoGen)
    99
    InvivoGen catalog code ant pr 1
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Catalog Code Ant Pr 1, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/Puromycin/bio_rxiv__2025__09__04__674188-145-19-22
    Average 99 stars, based on 1 article reviews
    catalog code ant pr 1 - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    sense probe targeting the reverse strand (coding strand- catalog# 425531)  (Advanced Cell Diagnostics Inc)
    90
    Advanced Cell Diagnostics Inc sense probe targeting the reverse strand (coding strand- catalog# 425531)
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Sense Probe Targeting The Reverse Strand (Coding Strand Catalog# 425531), supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/sense+probe+targeting+the+reverse+strand++coding+strand++catalog++425531+/pm40593491-365-9-19
    Average 90 stars, based on 1 article reviews
    sense probe targeting the reverse strand (coding strand- catalog# 425531) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    catalog codes ccl 23  (ATCC)
    99
    ATCC catalog codes ccl 23
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Catalog Codes Ccl 23, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/HEp-2/pm40362414-650-17-11
    Average 99 stars, based on 1 article reviews
    catalog codes ccl 23 - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    assay catalog code thpd nfis  (InvivoGen)
    98
    InvivoGen assay catalog code thpd nfis
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Assay Catalog Code Thpd Nfis, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/THP1-Dual+Cells/us12036231-660-7-4
    Average 98 stars, based on 1 article reviews
    assay catalog code thpd nfis - by Bioz Stars, 2026-11
    98/100 stars
    		  Buy from Supplier
    serum substitutes catalog code no. 10828028  (Thermo Fisher)
    90
    Thermo Fisher serum substitutes catalog code no. 10828028
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Serum Substitutes Catalog Code No. 10828028, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/serum+substitutes+catalog+code+no++10828028/pm38666419-87-17-23
    Average 90 stars, based on 1 article reviews
    serum substitutes catalog code no. 10828028 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    ap catalog code vac phos 250  (InvivoGen)
    95
    InvivoGen ap catalog code vac phos 250
    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli <t>K12</t> (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .
    Ap Catalog Code Vac Phos 250, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/catalog+code/Adju-Phos+adjuvant/pm38250834-83-5-12
    Average 95 stars, based on 1 article reviews
    ap catalog code vac phos 250 - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli K12 (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .

    Journal: PLOS Biology

    Article Title: Uracil-driven ROS signaling and larval TrpA1-B neuron activation are required for priming the adult Drosophila gustatory response to bacteria

    doi: 10.1371/journal.pbio.3004042

    Figure Lengend Snippet: (A) Live L. brevis are necessary and sufficient to prime the larvae. Sterilized eggs were exposed to live or dead bacteria as well as supernatant. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose (Sucrose 1 mM) and sucrose + PGN from E. coli K12 (Sucrose 1mM + PGN200) were obtained. (B) Uracil is necessary and sufficient to prime the larvae. Sterilized eggs were exposed to the different treatments and the resulting adults raised on antibiotics-containing media (ATB). Specifically, eggs were either monoassociated with bacterial strains, or exposed to LB media or to LB media containing uracil or cytosine. Addition of uracil or Ecc 15 24h after sterilized egg deposition was tested. The resulting adults were raised on antibiotics-containing media (ATB). PER index of w − flies to control solutions of sucrose and sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) were obtained. (C) The Duox enzyme is necessary in enterocytes for the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of GAL4 ( mex - GAL4 /+) and UAS (+/UAS-Duox_IR ) control flies as well as animals with the Duox transcript impaired via RNAi in enterocytes ( mex - GAL4 / UAS-Duox_IR ) were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (D) Graphical representation of the life periods during which flies are shifted from 18 °C to 29 °C for a stage-dependent RNAi assay. The ubiquitously expressed Tub-G80 ts , that inhibits the activity of GAL4, is temperature-sensitive: the GAL4 inhibitor is active at 18 °C (green) and inactivated at 29 °C (black), its inactivation allows the expression of any UAS . In the case of mex - GAL4 ; TubG80 ts /UAS-Duox_IR , at 18 °C the Duox transcripts will not be impaired (Duox) while it will be at 29 °C. (E) Duox activity in enterocytes is necessary during the larval life for priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of mex-GAL4; TubG80 ts /UAS-Duox_IR flies with the Duox transcript impaired all-life long, only during the larval stages or only during the adult stage were calculated. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (F) H 2 O 2 is detectable within the larvae 24 h following exposure to uracil 1 mM. Sterilized eggs were exposed to LB media or to LB media containing uracil. Twenty-four h later, the concentration of H 2 O 2 within the animals was quantified. To confirm that H 2 O 2 is the quantified product, catalase (0.01 U/mL and 0.001 U/mL) was applied to the samples from uracil-exposed larvae or overexpressed in enterocytes ( mex-GAL4/UAS-Catalase ). (G) Reducing the amount of ROS impairs the priming. PER index to solutions of sucrose and sucrose + PGN (PGN200) of UAS (+/UAS-Catalase ) control flies as well as animals with the Catalase over-expressed in enterocytes ( mex-GAL4/UAS-Catalase ) were obtained. Larvae were raised on conventional media and the resulting adults raised on antibiotics-containing media. (H) H 2 O 2 supplementation to larvae is not sufficient to prime while vitamin C (VitC) addition during the larval life prevents it. PER index to solutions of sucrose + PGN (PGN200) of w − flies was calculated. Larvae were raised on conventional media with or without vitamin C (0.2 mg/mL) or on monoassociation media with or without H 2 O 2 (1% on eggs or 1% on eggs then 1% on 24h-larvae (2X) or 2% on eggs). All the resulting adults were raised on antibiotics-containing media. PER index of w − flies to solutions of sucrose + PGN from E. coli K12 at 200 µg/mL (PGN200) was obtained. The PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% confidence interval (CI). A PER value of 1 means that 100% of the tested flies extended their proboscis following contact with the mixture, a value of 0.2 means that 20% of the animals extended their proboscis. The number of tested flies ( n ) is indicated on top of each bar. For each condition, at least 3 groups with a minimum of 10 flies per group were used. Each independent group is represented as an open circle. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 Fisher’s exact test. Further details can be found in the detailed lines, conditions and, statistics for the figure section. The data underlying this Figure can be found in . Created in BioRender. Kurz, L. (2026). https://core.local.biorender.dev/api/short-link/9ojribp .

    Article Snippet: PGN is E. coli K12: Invivogen, catalog code #tlrl-kipgn.

    Techniques: Bacteria, Control, RNAi Assay, Activity Assay, Inactivation, Expressing, Concentration Assay