casein blocking buffer (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher casein blocking buffer
Casein Blocking Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/casein+blocking+buffer/Casein+blocking+buffer/us12600767-508-25-28
Average 96 stars, based on 1 article reviews

casein blocking buffer - by Bioz Stars, 2026-10

96/100 stars

Images

Incubation:

Article Title: Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model
Article Snippet: The sections were allowed to acclimate to RT and fixed with 4% paraformaldehyde for 15 min (Electron Microscopy Sciences, 15710-S). .. The next day, they were washed three times with the wash buffer (0.1% Triton X-100 in PBS) and incubated with a blocking solution [50% Cas-Block (Thermo Fisher Scientific, 008120) and 50% PBS-T] for 15 min. Then, the sections were incubated with AF-488 (Thermo Fisher Scientific, A11039)–, AF-594 (Thermo Fisher Scientific, A11012)–, and/or AF-647 (Thermo Fisher Scientific, A32728)–conjugated secondary antibodies specific for the host species of the primary antibodies for 1 hour at RT in the dark. .. The samples were then incubated with a blocking solution for 1 hour at RT, and they were then incubated with primary antibodies [SOX9 (Merck, AB5535) and TWIST1 (Abcam, ab50887)] diluted in a blocking buffer overnight at 4°C.

Article Title: Knockout of Perilipin-2 in Microglia Alters Lipid Droplet Accumulation and Response to Alzheimer’s Disease Stimuli
Article Snippet: Equal protein (15 μg) was denatured in 4× Laemmli buffer with 5% β-mercaptoethanol (95 °C, 5 min), separated on 4–20% Criterion TGX precast gels (Bio-Rad Laboratories, Hercules, CA, USA; 5671094) at 180 V for 50–65 min, and transferred to nitrocellulose using Trans-Blot Turbo midi transfer packs (Bio-Rad Laboratories, Hercules, CA, USA; 17041590) with the manufacturer’s rapid program (~7 min). .. Membranes were blocked in casein blocking buffer (Thermo Fisher Scientific, Waltham, MA, USA; PI37528) for 1 h at room temperature and incubated overnight at 4 °C with rabbit anti-PLIN2/ADRP (Novus Biologicals, Centennial, CO, USA; NB110-40877, 1:800) diluted in blocking buffer; after three 5-min washes in PBS-T, membranes were incubated for 1 h at room temperature (protected from light) with StarBright Blue 700 goat anti-rabbit IgG (Bio-Rad, 12004161; 1:1000), washed, and imaged on a ChemiDoc system using the near-IR 700-nm channel with exposures set within the linear range. ..

Article Title: Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model
Article Snippet: The samples were stored at 4°C in the dark (up to 2 weeks) before imaging on a Leica SP8 inverted confocal microscope. .. This was followed by incubating budoids with 1× PBS for 10 min. To permeabilize samples, budoids were incubated with PBS-T for 3 × 10 min. Budoids were then incubated in a blocking solution [50% Cas-Block (Thermo Fisher Scientific, 008120) and 50% PBS-T] for 30 min. Budoids were then incubated with primary antibodies [TP63 (Abcam, ab735), SOX9 (Merck, AB5535), and E-CADHERIN (BD, 610405)] diluted in a blocking buffer overnight at 4°C. .. For whole-mount budoid immunostaining, budoids were collected from 96-well plates using low binding tips (Sigma-Aldrich, Z719668), with a cut tip, and handled with low binding tips throughout the protocol.

Article Title: βc receptor antagonism mitigates sarcoidosis granuloma formation by targeting inflammatory signals and aberrant lipid metabolism
Article Snippet: .. Sections were blocked in normal goat serum in CAS-Block (blocking buffer, Thermo Fisher Scientific, USA) for at least 30min in a humidified chamber at room temperature before mouse anti-CD68 antibodies (Invitrogen USA, KP1, 1:200 in blocking buffer) were added to the section and incubated overnight at 4°C. .. Sections were washed in distilled water, and Alexa Fluor ® -555 goat anti-mouse secondary antibody (Thermo Fisher Scientific USA, 1:400 in blocking buffer) was added to the section and incubated for 1h at room temperature in the humidified chamber in the dark.

Article Title: Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model
Article Snippet: The samples were stained with 1× PBS with Hoechst 33342 (Sigma-Aldrich, 2261) and washed in 1× PBS for 10 min. Stained budoids were transferred to imaging plates and stored at 4°C in the dark (up to 2 weeks) before imaging on a Leica SP8 inverted confocal microscope. .. Then, the samples were incubated with a blocking solution [50% Cas-Block (Thermo Fisher Scientific, 008120) and 50% PBS-T] for 30 min. .. The samples were then incubated with a TP63 primary antibody (Abcam, ab735) diluted in blocking buffer overnight at 4°C.

Blocking Assay:

Article Title: Molecular insights into treatment-resistant erectile dysfunction: staining patterns of human corpora cavernosa—a preliminary study
Article Snippet: Antigen retrieval was performed in citrate buffer, pH 6.0 (Novus, Centennial, CO, USA; NB900-62075). .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide (VWR ® , Radnor, PA, USA; BDH7690-1), and nonspecific binding was minimized using CAS blocking solution (Life Technologies, Carlsbad, CA, USA; 008120). ..

Article Title: Compounds and methods targeting epiregulin
Article Snippet: Mono Fc EREG fusions (see above) were diluted to 1 μg/mL in PBS, and 100 μL was added to each well to coat an ELISA plate (Greiner Cat #655061) overnight at 5° C. Variants were grouped by column on the plate with wild type coated in 3 replicate columns and the point mutants coated in single replicate columns. .. Following the coat, the plate was washed 3×200 μL per well with PBST, then blocked at RT for 1 hour on a plate shaker using casein blocking buffer (Thermo Cat #37528). ..

Activity Assay:

Binding Assay:

other:

Article Title: Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model
Article Snippet: For immunostaining in 2D cultures, the induction protocol was performed in 12-well glass-like polymer coverslip-bottom plates (Cellvis P12-1.5P).

Review