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Santa Cruz Biotechnology ca i
Ca I, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca/CA+I+Antibody/pmc13039253-6-0-3
Average 93 stars, based on 84 article reviews
ca i - by Bioz Stars, 2026-11
93/100 stars

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Western Blot:

Article Title: The Differentiation of Proteome Analysis of Omental Adipose Tissue, Placenta and Skeletal Muscle in between Pregnant Women with Gestational Diabetes and Type 1 Diabetes Mellitus
Article Snippet: .. The identi ed proteins were validated by western blot analysis using speci c primary antibodies against carbonic anhydrase (1:500, Santa Cruz, sc-393490), superoxide dismutase (1 : 1000, Santa Cruz, sc-271014), glyceraldehyde-3-phosphate dehydrogenase (1:1,000, Santa Cruz, sc-47724), alpha enolase (1 : 5000, Santa Cruz, sc-101513), creatine kinase-M (1 : 5000, Santa Cruz, sc-69848), and β-actin (1:2,500, Santa Cruz, sc-8432). β-Actin was used as an internal control to ensure equal protein loading. .. The membranes were visualized with an ECL detection system (Bio-Rad, USA) using X-ray lms and a VersaDoc imaging system (Bio-Rad, USA).

Control:

Article Title: The Differentiation of Proteome Analysis of Omental Adipose Tissue, Placenta and Skeletal Muscle in between Pregnant Women with Gestational Diabetes and Type 1 Diabetes Mellitus
Article Snippet: .. The identi ed proteins were validated by western blot analysis using speci c primary antibodies against carbonic anhydrase (1:500, Santa Cruz, sc-393490), superoxide dismutase (1 : 1000, Santa Cruz, sc-271014), glyceraldehyde-3-phosphate dehydrogenase (1:1,000, Santa Cruz, sc-47724), alpha enolase (1 : 5000, Santa Cruz, sc-101513), creatine kinase-M (1 : 5000, Santa Cruz, sc-69848), and β-actin (1:2,500, Santa Cruz, sc-8432). β-Actin was used as an internal control to ensure equal protein loading. .. The membranes were visualized with an ECL detection system (Bio-Rad, USA) using X-ray lms and a VersaDoc imaging system (Bio-Rad, USA).

Incubation:

Article Title: Microbiota shape the colon epithelium controlling inter-crypt absorptive goblet cells via butyrate–GP R109A signalling
Article Snippet: .. After sodium citrate antigen retrieval, primary antibodies against CHGA (ThermoFisher, #MA5-42925), CA1 (Santa Cruz Biotechnology #sc-393490), FABP2 (R&D Systems #AF1486), CLCA1 (Abcam #ab180851), LY6G (BioLegend #108408), MCT1 (Santa Cruz Biotechnology #sc365501), MCT4 (Santa Cruz Biotechnology #sc-376465), OATP (Santa Cruz Biotechnology #sc-271157), or KI67 (Abcam # ab15580) were diluted in antibody diluent (Dako #S0809) at 1:200 dilution for most antibodies (1:2000 for KI67), and the tissue sections were incubated with the antibody dilutions overnight. ..

Article Title: Sepsis prevents the development of experimental type 1 diabetes
Article Snippet: Endogenous peroxidase was blocked with commercial blocker (Envision FLEX, Dako, Glostrup, Denmark). .. Slides were then incubated overnight with primary antibodies (anti-insulin (H-86) antibody 1:500; anti-glucagon (FL-180) antibody 1:500; both from Santa Cruz, CA), followed by incubation with peroxidase-conjugated secondary antibodies (Envision FLEX HRP, Dako) according to the manufacturer’s instructions. .. Staining was developed with diaminobenzidine (Envision FLEX, Dako).

Article Title: Cerium doping of 45S5 bioactive glass improves redox potential and cellular bioactivity.
Article Snippet: The fixed cells were rinsed with PBS, permeabilized with 0.1% Triton X-100 (Sigma–Aldrich, St. Louis, MO, United States), and blocked using 1% bovine serum albumin for 30 min at 25 °C. .. After blocking, the cells were incubated for overnight at 4 °C with COL-I primary antibody (1:500, Santa Cruz Biotechnology, Santa Cruz, CA, United States) in the dark. .. After incubation, the cells were rinsed with PBS and incubated in a secondary antibody (Alexa Fluor 488 conjugated Goat and anti-mouse IgG H&L, ThermoFisher Scientific, Waltham, MA, United States).

Formalin-fixed Paraffin-Embedded:

Article Title: Microbiota shape the colon epithelium controlling inter-crypt absorptive goblet cells via butyrate–GP R109A signalling
Article Snippet: .. Sections from formalin-fixed paraffin-embedded (FFPE) samples were used for immunofluorescence with primary antibodies against MUC2 (Santa Cruz Biotechnology #sc-7314, 1:50) and CA1 (Santa Cruz Biotechnology #sc-393490, 1:200). ..

Immunofluorescence:

Article Title: Microbiota shape the colon epithelium controlling inter-crypt absorptive goblet cells via butyrate–GP R109A signalling
Article Snippet: .. Sections from formalin-fixed paraffin-embedded (FFPE) samples were used for immunofluorescence with primary antibodies against MUC2 (Santa Cruz Biotechnology #sc-7314, 1:50) and CA1 (Santa Cruz Biotechnology #sc-393490, 1:200). ..

Blocking Assay:

Article Title: Cerium doping of 45S5 bioactive glass improves redox potential and cellular bioactivity.
Article Snippet: The fixed cells were rinsed with PBS, permeabilized with 0.1% Triton X-100 (Sigma–Aldrich, St. Louis, MO, United States), and blocked using 1% bovine serum albumin for 30 min at 25 °C. .. After blocking, the cells were incubated for overnight at 4 °C with COL-I primary antibody (1:500, Santa Cruz Biotechnology, Santa Cruz, CA, United States) in the dark. .. After incubation, the cells were rinsed with PBS and incubated in a secondary antibody (Alexa Fluor 488 conjugated Goat and anti-mouse IgG H&L, ThermoFisher Scientific, Waltham, MA, United States).



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