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Image Search Results
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.
doi: 10.1016/j.biopha.2019.109578
Figure Lengend Snippet: Fig. 1. Identification of CA8 expression between normal and RCC tissue. A, CA8 mRNA expression levels, CA8 level were measured by real-time PCR (with nor- malization relative to GAPDH levels). Data shown are fold-change relative to the CA8 levels in normal tissue, mean ± SD. *P < 0.05 B, Representative im- munohistochemical staining are shown for CA8 protein, Scale bars: 50 μm. C, CA8 IHC quantification data measuring average optical density using ImageJ d 1.47 software. D, TCGA database analysis of CA8 mRNA expression level in RCC based on individual cancer stages. * p < 0.05 represents the comparison between adjacent normal and RCC tissue, t-test.
Article Snippet:
Techniques: Expressing, Real-time Polymerase Chain Reaction, Staining, Software, Comparison
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.
doi: 10.1016/j.biopha.2019.109578
Figure Lengend Snippet: Fig. 2. Overexpression of CA8 promoted Caki-1 proliferation and migration. A, CA8 protein level in Caki-1 was increased significantly after being transfected with CA8 plasmids, MMP2 and pAKT protein levels were also analyzed by immunoblotting; GAPDH was employed a loading control. B, CA8-overexpression efficiency was evaluated by RT-PCR (normalization relative to GAPDH levels). Data shown are fold change relative to the CA8 levels in control cells, mean ± SD. *P < 0.01. C, CA8-overexpression could promote Caki-1 proliferation, mean ± SD. *P < 0.05. D, CA8-overexpression could increase Caki-1 migration, mean ± SD. *P < 0.05.
Article Snippet:
Techniques: Over Expression, Migration, Transfection, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.
doi: 10.1016/j.biopha.2019.109578
Figure Lengend Snippet: Fig. 3. CA8-knockdown decreased Caki-1 proliferation and migration. A, CA8 protein level in Caki-1 was reduced significantly after being transfected with CA8- shRNA, MMP2 and pAKT protein levels were also analyzed by immunoblotting; GAPDH was employed a loading control. B, CA8-knockdown efficiency was evaluated by RT-PCR (normalization relative to GAPDH levels). Data shown are fold change relative to the CA8 levels in control cells, mean ± SD. *P < 0.01. C, CA8- knockdown could restrains Caki-1 proliferation, mean ± SD. *P < 0.05. D, CA8-knockdown could inhibit Caki-1 migration, mean ± SD. *P < 0.05.
Article Snippet:
Techniques: Knockdown, Migration, Transfection, shRNA, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.
doi: 10.1016/j.biopha.2019.109578
Figure Lengend Snippet: Fig. 4. Overexpression of CA8 promoted Caki-1 xenograft growth. A, tumors formed by CA8-overexpressed Caki-1 emerged earlier and grown faster than tumors formed by vector control-Caki-1 cells, mean ± SD. *P < 0.05. B, CA8-overexpressed Caki-1 cells expressed more Ki67 protein than the control group, mean ± SD. *P < 0.05.
Article Snippet:
Techniques: Over Expression, Plasmid Preparation, Control