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oxygen controller  (BioSpherix)


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    BioSpherix oxygen controller
    Oxygen Controller, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c21/ProOx+C21/pmc13157167-264-20-17
    Average 96 stars, based on 236 article reviews
    oxygen controller - by Bioz Stars, 2026-11
    96/100 stars

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    other:

    Article Title: Metformin protects against myocardial ischemia/reperfusion injury and suppresses the Nur77-SPARCL1 inflammatory axis.
    Article Snippet: Myocardial ischemia/reperfusion (I/R) injury triggers sterile inflammation that amplifies tissue damage.. We investigated whether metformin limits this response through the Nur77–SPARCL1 axis.. RNA sequencing of HL-1 cardiomyocytes (n = 3 biological replicates per group) identified Sparcl1 as a metformin-responsive transcript.

    Article Title: Immune‐Responsive Bioinks Enable Vision‐Guided in Situ Robotic Bioprinting to Accelerate Diabetic Wound Healing
    Article Snippet: Chronic diabetic wounds remain amajor clinical challenge because persistent inflammation, hypoxia, and immune dysregulation prevent the transition of macrophages from pro-inflammatory M1 states to pro-regenerative M2 phenotypes.. Here, we report an immunomodulatory colloidal bioink that integrates zein-based oxygen-generating microparticles and human mesenchymal stem cells within a porous, mechanically robust matrix for adaptive in situ bioprinting.. Implemented with the autonomous, intelligent, visually guided in situ robotic bioprinting platform, this bioink enables patient-specific deposition directly onto wound defects with high spatial conformity and stable tissue integration.

    Article Title: Direct interaction between miR-210-5p and HIF-1α regulates HIF-dependent transcription
    Article Snippet: Hypoxic stimulation was performed at 1% O2, 5% CO2, and 94% N2 using a ProOx C21 hypoxia chamber (BioSpherix).

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxia in human brain organoids
    Article Snippet: COs were exposed to low oxygen conditions on day 120 employing a hypoxic chamber (C-Chamber Three Shelf, Biospherix) and an oxygen controller (Pro Ox C21 Oxygen CO2 Single Chamber Controller).

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxic in human brain organoids
    Article Snippet: 611 Hypoxia modelling 612 613 COs were exposed to low oxygen conditions on day 120 employing a hypoxic chamber (C-Chamber 614 Three Shelf, Biospherix) and an oxygen controller (Pro Ox C21 Oxygen CO2 Single Chamber 615 Controller).

    Cell Culture:

    Article Title: EPAS1 knockdown is associated with cell cycle and DNA replication programs and MYC/E2F-related signatures in hemangioma endothelial cells
    Article Snippet: .. Cells were cultured under normoxic (21% O2, 5% CO2) or hypoxic (1% O2, 5% CO2, 94% N2) conditions in CO2 incubators (Thermo Fisher), with hypoxia maintained using a ProOx C21 O2 controller (BioSpherix). ..

    Incubator:

    Article Title: TH1834-Mediated TIP60 Inhibition Protects Against Ischemia/Reperfusion Damage in Human iPSC-Derived Cardiomyocytes and Cardioids
    Article Snippet: .. H/R injury was induced using a hypoxia incubator (Biospherix, ProOX C21) with continuous oxygen monitoring. ..

    Control:

    Article Title: Optimized Ex Vivo Human Liver Slice Culture Maintains Extended Viability and Function for Hepatotoxicity Testing.
    Article Snippet: Slices were subsequently cultured in liver tissue culture medium composed of William’s Medium E (WME) with L-glutamine (Thermo Fisher Scientific, Cat# A1217601), supplemented with D-glucose (14 m M ; Sigma– Aldrich, Cat# G8 769), penicillin (100 U/mL) and streptomycin (100 μg/mL; Gibco, Cat# 15140122), insulin (30 n M ; Sigma– Aldrich, Cat# I9278), glucagon (100 n M ; Sigma–Aldrich, Cat# G2044), corticosterone (1 μM ; Sigma–Aldrich, Cat# C2505), epidermal growth factor (EGF, 1 n M ; PeproTech, Cat# AF-100-15), and human AB serum (5%; Sigma–Aldrich, Cat# H4522). .. All experiments under controlled oxygen conditions were conducted in an O2 /CO2 control chamber (ProOx Model C21; BioSpherix). .. Tisue cores were obtained using a 6-mm Stiefel biopsy punch, transerred to a metal mold, embedded in 3% low-gelling-temperature garose, and placed on ice for 2–5 min. Agarose-embedded cores ere sectioned using a Leica VT1200S vibrating blade vibratome t a speed of 0.3 mm/s, amplitude of 1.3 mm, and thickness anging from 150 to 350 μm.



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    (A) Experimental scheme showing rectal AKP transplantation into Lepr-Cre(+);Chrm3 fl/fl and control mice. IVIS, in vivo imaging. (B and C) Mouse survival (B) and tumor-derived luciferase signals (C). n=6 ( Lepr-Cre(−);Chrm3 fl/fl ) and 9 mice ( Lepr-Cre(+);Chrm3 fl/fl ). (D) Non-phosphorylated YAP/PDGFRA co-IF. Red arrowheads; non-phosphorylated (active) YAP in PDGFRA + CAFs. cKO, conditional KO. n=5 mice. (E) NTN1/PDGFRA co-IF. n=5 mice. (F) VAChT IHC. Red arrowheads; VAChT + nerves. n=5 mice. (G) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist <t>C21</t> (0.03 mg/kg) from Day 8. (H and I) Mouse survival (H) and tumor-derived luciferase signals (I). n=8 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 fl/fl ), and 7 mice ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl ). (J) Non-phosphorylated YAP/PDGFRA co-IF. Red/purple arrowheads; non-phosphorylated YAP in PDGFRA + CAFs in the Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl groups. n=4 mice. (K) NTN1/PDGFRA co-IF. n=4 mice. (L) VAChT IHC. Green/red arrowheads:VAChT + nerves in the Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT groups. n=4 mice. Scale bars, 100 μm (A and G), 50/5 μm (main/insets; D, E, J, and K), 50 μm (F and L). Log-rank test (B and H), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (C and I), two-tailed unpaired Student’s t-test (D-F), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (J-L). Mean±s.e.m. (C-F and I-L). See also Figures S5 .
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    (A) Experimental scheme showing rectal AKP transplantation into Lepr-Cre(+);Chrm3 fl/fl and control mice. IVIS, in vivo imaging. (B and C) Mouse survival (B) and tumor-derived luciferase signals (C). n=6 ( Lepr-Cre(−);Chrm3 fl/fl ) and 9 mice ( Lepr-Cre(+);Chrm3 fl/fl ). (D) Non-phosphorylated YAP/PDGFRA co-IF. Red arrowheads; non-phosphorylated (active) YAP in PDGFRA + CAFs. cKO, conditional KO. n=5 mice. (E) NTN1/PDGFRA co-IF. n=5 mice. (F) VAChT IHC. Red arrowheads; VAChT + nerves. n=5 mice. (G) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist <t>C21</t> (0.03 mg/kg) from Day 8. (H and I) Mouse survival (H) and tumor-derived luciferase signals (I). n=8 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 fl/fl ), and 7 mice ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl ). (J) Non-phosphorylated YAP/PDGFRA co-IF. Red/purple arrowheads; non-phosphorylated YAP in PDGFRA + CAFs in the Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl groups. n=4 mice. (K) NTN1/PDGFRA co-IF. n=4 mice. (L) VAChT IHC. Green/red arrowheads:VAChT + nerves in the Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT groups. n=4 mice. Scale bars, 100 μm (A and G), 50/5 μm (main/insets; D, E, J, and K), 50 μm (F and L). Log-rank test (B and H), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (C and I), two-tailed unpaired Student’s t-test (D-F), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (J-L). Mean±s.e.m. (C-F and I-L). See also Figures S5 .
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    (A) Experimental scheme showing rectal AKP transplantation into Lepr-Cre(+);Chrm3 fl/fl and control mice. IVIS, in vivo imaging. (B and C) Mouse survival (B) and tumor-derived luciferase signals (C). n=6 ( Lepr-Cre(−);Chrm3 fl/fl ) and 9 mice ( Lepr-Cre(+);Chrm3 fl/fl ). (D) Non-phosphorylated YAP/PDGFRA co-IF. Red arrowheads; non-phosphorylated (active) YAP in PDGFRA + CAFs. cKO, conditional KO. n=5 mice. (E) NTN1/PDGFRA co-IF. n=5 mice. (F) VAChT IHC. Red arrowheads; VAChT + nerves. n=5 mice. (G) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist <t>C21</t> (0.03 mg/kg) from Day 8. (H and I) Mouse survival (H) and tumor-derived luciferase signals (I). n=8 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 fl/fl ), and 7 mice ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl ). (J) Non-phosphorylated YAP/PDGFRA co-IF. Red/purple arrowheads; non-phosphorylated YAP in PDGFRA + CAFs in the Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl groups. n=4 mice. (K) NTN1/PDGFRA co-IF. n=4 mice. (L) VAChT IHC. Green/red arrowheads:VAChT + nerves in the Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT groups. n=4 mice. Scale bars, 100 μm (A and G), 50/5 μm (main/insets; D, E, J, and K), 50 μm (F and L). Log-rank test (B and H), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (C and I), two-tailed unpaired Student’s t-test (D-F), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (J-L). Mean±s.e.m. (C-F and I-L). See also Figures S5 .
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    biospherix proox c21 system  (BioSpherix)
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    BioSpherix biospherix proox c21 system
    (A) Experimental scheme showing rectal AKP transplantation into Lepr-Cre(+);Chrm3 fl/fl and control mice. IVIS, in vivo imaging. (B and C) Mouse survival (B) and tumor-derived luciferase signals (C). n=6 ( Lepr-Cre(−);Chrm3 fl/fl ) and 9 mice ( Lepr-Cre(+);Chrm3 fl/fl ). (D) Non-phosphorylated YAP/PDGFRA co-IF. Red arrowheads; non-phosphorylated (active) YAP in PDGFRA + CAFs. cKO, conditional KO. n=5 mice. (E) NTN1/PDGFRA co-IF. n=5 mice. (F) VAChT IHC. Red arrowheads; VAChT + nerves. n=5 mice. (G) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist <t>C21</t> (0.03 mg/kg) from Day 8. (H and I) Mouse survival (H) and tumor-derived luciferase signals (I). n=8 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 fl/fl ), and 7 mice ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl ). (J) Non-phosphorylated YAP/PDGFRA co-IF. Red/purple arrowheads; non-phosphorylated YAP in PDGFRA + CAFs in the Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl groups. n=4 mice. (K) NTN1/PDGFRA co-IF. n=4 mice. (L) VAChT IHC. Green/red arrowheads:VAChT + nerves in the Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT groups. n=4 mice. Scale bars, 100 μm (A and G), 50/5 μm (main/insets; D, E, J, and K), 50 μm (F and L). Log-rank test (B and H), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (C and I), two-tailed unpaired Student’s t-test (D-F), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (J-L). Mean±s.e.m. (C-F and I-L). See also Figures S5 .
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    Image Search Results


    (A) Experimental scheme showing rectal AKP transplantation into Lepr-Cre(+);Chrm3 fl/fl and control mice. IVIS, in vivo imaging. (B and C) Mouse survival (B) and tumor-derived luciferase signals (C). n=6 ( Lepr-Cre(−);Chrm3 fl/fl ) and 9 mice ( Lepr-Cre(+);Chrm3 fl/fl ). (D) Non-phosphorylated YAP/PDGFRA co-IF. Red arrowheads; non-phosphorylated (active) YAP in PDGFRA + CAFs. cKO, conditional KO. n=5 mice. (E) NTN1/PDGFRA co-IF. n=5 mice. (F) VAChT IHC. Red arrowheads; VAChT + nerves. n=5 mice. (G) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist C21 (0.03 mg/kg) from Day 8. (H and I) Mouse survival (H) and tumor-derived luciferase signals (I). n=8 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 fl/fl ), and 7 mice ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl ). (J) Non-phosphorylated YAP/PDGFRA co-IF. Red/purple arrowheads; non-phosphorylated YAP in PDGFRA + CAFs in the Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl groups. n=4 mice. (K) NTN1/PDGFRA co-IF. n=4 mice. (L) VAChT IHC. Green/red arrowheads:VAChT + nerves in the Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT groups. n=4 mice. Scale bars, 100 μm (A and G), 50/5 μm (main/insets; D, E, J, and K), 50 μm (F and L). Log-rank test (B and H), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (C and I), two-tailed unpaired Student’s t-test (D-F), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (J-L). Mean±s.e.m. (C-F and I-L). See also Figures S5 .

    Journal: Cancer cell

    Article Title: Cholinergic signaling via muscarinic receptor 3 promotes colorectal cancer progression directly and indirectly through stroma-derived Netrin-1

    doi: 10.1016/j.ccell.2026.06.018

    Figure Lengend Snippet: (A) Experimental scheme showing rectal AKP transplantation into Lepr-Cre(+);Chrm3 fl/fl and control mice. IVIS, in vivo imaging. (B and C) Mouse survival (B) and tumor-derived luciferase signals (C). n=6 ( Lepr-Cre(−);Chrm3 fl/fl ) and 9 mice ( Lepr-Cre(+);Chrm3 fl/fl ). (D) Non-phosphorylated YAP/PDGFRA co-IF. Red arrowheads; non-phosphorylated (active) YAP in PDGFRA + CAFs. cKO, conditional KO. n=5 mice. (E) NTN1/PDGFRA co-IF. n=5 mice. (F) VAChT IHC. Red arrowheads; VAChT + nerves. n=5 mice. (G) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist C21 (0.03 mg/kg) from Day 8. (H and I) Mouse survival (H) and tumor-derived luciferase signals (I). n=8 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT ), 7 ( Lepr-Cre;LSL-hM3Dq(−);Ntn1 fl/fl ), and 7 mice ( Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl ). (J) Non-phosphorylated YAP/PDGFRA co-IF. Red/purple arrowheads; non-phosphorylated YAP in PDGFRA + CAFs in the Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 fl/fl groups. n=4 mice. (K) NTN1/PDGFRA co-IF. n=4 mice. (L) VAChT IHC. Green/red arrowheads:VAChT + nerves in the Lepr-Cre;LSL-hM3Dq(−);Ntn1 WT / Lepr-Cre;LSL-hM3Dq(+);Ntn1 WT groups. n=4 mice. Scale bars, 100 μm (A and G), 50/5 μm (main/insets; D, E, J, and K), 50 μm (F and L). Log-rank test (B and H), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (C and I), two-tailed unpaired Student’s t-test (D-F), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (J-L). Mean±s.e.m. (C-F and I-L). See also Figures S5 .

    Article Snippet: A Hamilton syringe holding a 33-gauge needle was used to inject C21 (0.03 mg/kg; HY-100234A, MedChemExpress; a volume of 10 μL, dissolved in DPBS) into the rectal tumor percutaneously through the anal skin.

    Techniques: Expressing, Transplantation Assay, Control, In Vivo Imaging, Derivative Assay, Luciferase, Injection, Comparison, Two Tailed Test, Immunohistochemistry

    (A) Luciferase (luc)-expressing AKP tumor organoids were co-treated with ACh and an AChR antagonist for 72 h. AKP tumor-derived luc signals were measured as a surrogate for viable cell numbers. Drug concentration as in Figure 2A . n=4. (B) Luc signals from control gRNA/g Chrm3 -expressing luc + AKP organoids treated (72 h). Bethanechol (Beth), 10 μM. n=4. (C) Gene set enrichment analysis of the YAP gene signature 53 using bulk RNA-seq from Beth-/vehicle-treated AKP organoids. The normalized enrichment score (NES) and false discovery rate (FDR)-adjusted P value are shown. n=3. (D) Western blotting of control gRNA/g Chrm3 -expressing AKP tumor cells treated (20 min). Red dotted rectangles: dephosphorylation of LATS1/YAP by ACh/Beth treatment. FR900359 (FR, Gq inhibitor), 1 μM. (E) Luc signals from luc + AKP organoids treated (72 h). VP, verteporfin (YAP inhibitor, 0.5 μM). n=5. (F) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist C21 (0.03 mg/kg) from Day 8. (G and H) Mouse survival (G) and tumor-derived luc signals (H). n=10 ( Chat-Cre(−);hM3Dq mice; control AKP), 9 ( Chat-Cre(+);hM3Dq mice; control AKP), 9 ( Chat-Cre(−);hM3Dq mice; g Chrm3 AKP), and 9 mice ( Chat-Cre(+);hM3Dq mice; g Chrm3 AKP). (I) Non-phosphorylated YAP/luc (a marker for luc + AKP cancer cells) co-IF. Red arrowheads; non-phosphorylated YAP in luc + cancer cells. n=5 mice. Scale bars, 100 μm (A and F) and 50/5 μm (main/insets; I). One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (A, B, and E), gene set permutation tests (C), log-rank test (G), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (H), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (I). Mean±s.e.m. (A, B, E, H, and I). See also Figures S6 and S7 ; Tables S3 and S4 .

    Journal: Cancer cell

    Article Title: Cholinergic signaling via muscarinic receptor 3 promotes colorectal cancer progression directly and indirectly through stroma-derived Netrin-1

    doi: 10.1016/j.ccell.2026.06.018

    Figure Lengend Snippet: (A) Luciferase (luc)-expressing AKP tumor organoids were co-treated with ACh and an AChR antagonist for 72 h. AKP tumor-derived luc signals were measured as a surrogate for viable cell numbers. Drug concentration as in Figure 2A . n=4. (B) Luc signals from control gRNA/g Chrm3 -expressing luc + AKP organoids treated (72 h). Bethanechol (Beth), 10 μM. n=4. (C) Gene set enrichment analysis of the YAP gene signature 53 using bulk RNA-seq from Beth-/vehicle-treated AKP organoids. The normalized enrichment score (NES) and false discovery rate (FDR)-adjusted P value are shown. n=3. (D) Western blotting of control gRNA/g Chrm3 -expressing AKP tumor cells treated (20 min). Red dotted rectangles: dephosphorylation of LATS1/YAP by ACh/Beth treatment. FR900359 (FR, Gq inhibitor), 1 μM. (E) Luc signals from luc + AKP organoids treated (72 h). VP, verteporfin (YAP inhibitor, 0.5 μM). n=5. (F) Experimental scheme showing rectal AKP transplantation with daily intratumor injection of the hM3Dq agonist C21 (0.03 mg/kg) from Day 8. (G and H) Mouse survival (G) and tumor-derived luc signals (H). n=10 ( Chat-Cre(−);hM3Dq mice; control AKP), 9 ( Chat-Cre(+);hM3Dq mice; control AKP), 9 ( Chat-Cre(−);hM3Dq mice; g Chrm3 AKP), and 9 mice ( Chat-Cre(+);hM3Dq mice; g Chrm3 AKP). (I) Non-phosphorylated YAP/luc (a marker for luc + AKP cancer cells) co-IF. Red arrowheads; non-phosphorylated YAP in luc + cancer cells. n=5 mice. Scale bars, 100 μm (A and F) and 50/5 μm (main/insets; I). One-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (A, B, and E), gene set permutation tests (C), log-rank test (G), two-way repeated-measures ANOVA with post-hoc Sidak’s multiple comparison test at week 2 (H), and two-way ANOVA followed by Tukey’s post-hoc multiple comparison tests (I). Mean±s.e.m. (A, B, E, H, and I). See also Figures S6 and S7 ; Tables S3 and S4 .

    Article Snippet: A Hamilton syringe holding a 33-gauge needle was used to inject C21 (0.03 mg/kg; HY-100234A, MedChemExpress; a volume of 10 μL, dissolved in DPBS) into the rectal tumor percutaneously through the anal skin.

    Techniques: Activation Assay, Luciferase, Expressing, Organoids, Derivative Assay, Concentration Assay, Control, Analysis, RNA Sequencing, Western Blot, De-Phosphorylation Assay, Transplantation Assay, Injection, Marker, Comparison