Journal: Journal of Medicine and Life

Article Title: Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT/PI3K expression

doi: 10.25122/jml-2022-0299

Figure Lengend Snippet: Renal tissue P-AKT staining scores of all groups measured by IHC; C21-treated group shows significantly improved P-AKT results compared to the sepsis control group (#P 0.05 vs. control).

Article Snippet: C21 was purchased from Medchem Express MCE in the USA and made in dimethyl sulfoxide (DMSO) as the standard vehicle before being diluted in N.S. at a ratio of 1:1 and prepared just before usage.

Techniques: Staining, Control

Journal: The Tohoku journal of experimental medicine

Article Title: Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer.

doi: 10.1620/tjem.241.239

Figure Lengend Snippet: Fig. 1. Expression of TBL1XR1 in serous EOC tissues. (A) Representative high expression of TBL1XR1 in EOC tissues, with the IHC score 9. (B) Representative low expres- sion of TBL1XR1 in EOC tissues, with the IHC score 0. (C) Western blot analysis showed the protein levels of TBL1XR1 in 8 pairs of EOC tissues (T) and adjacent non-tumorous tissues (ANT). (D) Quantification of the protein levels of TBL1XR1 with Graphpad prism 5.0 software. (E) RT-qPCR results demonstrated that the mRNA level of TBL1XR1 was higher in tumor tissues than that in adjacent non-tumorous tissues (n = 8). Magnification: 200X.

Article Snippet: The Myc-tagged human TBL1XR1 cDNA was obtained from Origene (NM_024665) and cloned into pcDNA3.0 vector for trans- fection.

Techniques: Expressing, Western Blot, Software, Quantitative RT-PCR

Journal: The Tohoku journal of experimental medicine

Article Title: Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer.

doi: 10.1620/tjem.241.239

Figure Lengend Snippet: Fig. 2. Correlations between TBL1XR1 and clinical characteristics of serous EOC patients. (A) EOC patients with advanced FIGO stages showed higher immunoreactivity of TBL1XR1 (P = 0.009). (B) Expres- sion levels of TBL1XR1 were higher in patients with positive lymph node metastasis than in those with negative lymph node metastasis (P = 0.002). (C) Kaplan-Meier survival curve showed that higher expression of TBL1XR1 was corre- lated with poorer overall survival of EOC patients. (D) The VEGF-C mRNA levels were positively correlated with TBL1XR1 mRNA expression in tumor tissues (The data was generated by RT-qPCR from 8 paired EOC tissues and adjacent non-tumorous tissues. X axis showed the fold changes of TBL1XR1 while Y axis showed the fold changes of VEGF-C in corresponding tissues).

Article Snippet: The Myc-tagged human TBL1XR1 cDNA was obtained from Origene (NM_024665) and cloned into pcDNA3.0 vector for trans- fection.

Techniques: Expressing, Generated, Quantitative RT-PCR

Journal: The Tohoku journal of experimental medicine

Article Title: Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer.

doi: 10.1620/tjem.241.239

Figure Lengend Snippet: Fig. 3. TBL1XR1 modulates the expression of VEGF-C in EOC cells. TBL1XR1 showed highest protein expression in OVCAR3 cells among Hs832, SKOV3, and OVCAR3 cell lines, whereas no signifi- cant difference was observed between Hs832 and SKOV3 cells (A, B). TBL1XR1 overexpression increased the protein level of VEGF-C (C, D). In contrast, TBL1XR1 silencing by siRNAs down-regulated the protein expression of VEGF-C (E, F). Each experi- ment was performed in triplicate.

Article Snippet: The Myc-tagged human TBL1XR1 cDNA was obtained from Origene (NM_024665) and cloned into pcDNA3.0 vector for trans- fection.

Techniques: Expressing, Over Expression

Journal: The Tohoku journal of experimental medicine

Article Title: Over-Expression of TBL1XR1 Indicates Poor Prognosis of Serous Epithelial Ovarian Cancer.

doi: 10.1620/tjem.241.239

Figure Lengend Snippet: Fig. 4. Role of TBL1XR1 in regulating proliferation and invasion of EOC cells. TBL1XR1 overexpression enhanced the proliferation ability of SKOV3 cells (A), and TBL1XR1 knock-down impaired the viability of OVCAR3 cells (B). Similarly, the invasion capacity of EOC cells was also positively correlated with the TBL1XR1 expression levels (C, D). Each experiment was performed in triplicate.

Article Snippet: The Myc-tagged human TBL1XR1 cDNA was obtained from Origene (NM_024665) and cloned into pcDNA3.0 vector for trans- fection.

Techniques: Over Expression, Knockdown, Expressing

Journal: iScience

Article Title: Elabela inhibits TRAF1/NF-κB induced oxidative DNA damage to promote diabetic foot ulcer wound healing

doi: 10.1016/j.isci.2023.107601

Figure Lengend Snippet:

Article Snippet: rabbit anti-p21 , Proteintech , Cat# 10335-1-AP.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software, Extraction

Journal: Russian Journal of Physical Chemistry

Article Title: Effect of Et2Zn on Binding Spike Protein of SARS-Cov-2 and ACE2 from Cell Membrane

doi: 10.1134/S0036024423050163

Figure Lengend Snippet: Donor–acceptor results, E2 (resonance energy), occupancy, hybrid for RBD/Gly-Phe/hAP and RBD/Cys-Glu/hAPN

Article Snippet: RBD/Cys-Glu/hAPN , 0.8923 , BD (1) Zn9–C21 s 1 p 0.43 d 0.18 , BD (1) Zn9–C2 (Donor) BD*(1) C21–O22 (Acceptor) , 0.86.

Techniques: