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human src enzyme  (Carna Inc)


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    Structured Review

    Carna Inc human src enzyme
    Human Src Enzyme, supplied by Carna Inc, used in various techniques. Bioz Stars score: 95/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c-src/SRC/us10479780-2133-23-28
    Average 95 stars, based on 41 article reviews
    human src enzyme - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    In Vitro:

    Article Title: Detecting Protein Higher-Order Structural Changes Using Kinase as a Phospho-Labeler
    Article Snippet: RNA digestion was performed based on the method described by Sindhuja et al . For the digestion, 2 μL of RNase cocktail (Thermo Fisher Scientific) was added per 100 μL of protein solution, followed by incubation at 4 °C for 30 minutes. .. The in vitro kinase reaction was performed using recombinant tyrosine kinase SRC (human, Carna Biosciences) at an enzyme-to-substrate (E:S) ratio of 1/1000 (w/w) in the presence of 1 mM ATP and 20 mM MgCl2 at 37 °C for 15 minutes. ..

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1.
    Article Snippet: After centrifugation, lysates were incubated with the anti-EGFP antibody at 4°C overnight and then rotated with Dynabeads Protein G (Thermo Fisher Scientific) at 4°C for 1.5 h. The beads were washed three times with wash buffer (1:1 mixture of whole-cell lysate buffer and dilution buffer). .. In vitro kinase assay The recombinant human GST-TAB1-C protein (WT and Y481F) derived from Escherichia coli (34) was reacted with the recombinant human active GST-Src kinase derived from insect cells (Carna Bioscience, Japan) at 30°C for 30 min in 30 μl of reaction buffer containing 20 mM HEPES (pH 7.6), 20 mM MgCl2, 0.2 mM ATP, 2 mM DTT, 20 mM β-glycerophosphate, and 0.1 mM sodium orthovanadate. .. Recombinant human 6×His-Src protein derived from E. coli (RayBiotech, GA) was reacted with the recombinant human active GST-p38α kinase derived from E. coli (Carna Bioscience, Japan).

    Recombinant:

    Article Title: Detecting Protein Higher-Order Structural Changes Using Kinase as a Phospho-Labeler
    Article Snippet: RNA digestion was performed based on the method described by Sindhuja et al . For the digestion, 2 μL of RNase cocktail (Thermo Fisher Scientific) was added per 100 μL of protein solution, followed by incubation at 4 °C for 30 minutes. .. The in vitro kinase reaction was performed using recombinant tyrosine kinase SRC (human, Carna Biosciences) at an enzyme-to-substrate (E:S) ratio of 1/1000 (w/w) in the presence of 1 mM ATP and 20 mM MgCl2 at 37 °C for 15 minutes. ..

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1
    Article Snippet: After centrifugation, lysates were incubated with the anti-EGFP antibody at 4 °C overnight and then rotated with Dynabeads Protein G (Thermo Fisher Scientific) at 4 °C for 1.5 h. The beads were washed three times with wash buffer (1:1 mixture of whole-cell lysate buffer and dilution buffer). .. The recombinant human GST-TAB1-C protein (WT and Y481F) derived from Escherichia coli ( ) was reacted with the recombinant human active GST-Src kinase derived from insect cells (Carna Bioscience) at 30 °C for 30 min in 30 μl of reaction buffer containing 20 mM HEPES (pH 7.6), 20 mM MgCl 2 , 0.2 mM ATP, 2 mM DTT, 20 mM β-glycerophosphate, and 0.1 mM sodium orthovanadate. .. Recombinant human 6 × His-Src protein derived from E. coli (RayBiotech, GA) was reacted with the recombinant human active GST-p38α kinase derived from E. coli (Carna Bioscience).

    Article Title: Discovery of Pyridine-2-Carboxamides Derivatives as Potent and Selective HPK1 Inhibitors for the Treatment of Cancer
    Article Snippet: .. The recombinant human SRC protein (final concentration 0.3nM, purchased from Carna Biosciences, Cat No, 08-173) was preincubated with serially diluted compounds (10000-0.50805 nM, 3-fold increments) in a reaction buffer [50 mM HEPES, pH 7.5; 10 mM MgCl2; 2 mM DTT; 0.01% Triton X-100; 0.01% BSA] for 10 min at room temperature. ..

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1.
    Article Snippet: After centrifugation, lysates were incubated with the anti-EGFP antibody at 4°C overnight and then rotated with Dynabeads Protein G (Thermo Fisher Scientific) at 4°C for 1.5 h. The beads were washed three times with wash buffer (1:1 mixture of whole-cell lysate buffer and dilution buffer). .. In vitro kinase assay The recombinant human GST-TAB1-C protein (WT and Y481F) derived from Escherichia coli (34) was reacted with the recombinant human active GST-Src kinase derived from insect cells (Carna Bioscience, Japan) at 30°C for 30 min in 30 μl of reaction buffer containing 20 mM HEPES (pH 7.6), 20 mM MgCl2, 0.2 mM ATP, 2 mM DTT, 20 mM β-glycerophosphate, and 0.1 mM sodium orthovanadate. .. Recombinant human 6×His-Src protein derived from E. coli (RayBiotech, GA) was reacted with the recombinant human active GST-p38α kinase derived from E. coli (Carna Bioscience, Japan).

    Derivative Assay:

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1
    Article Snippet: After centrifugation, lysates were incubated with the anti-EGFP antibody at 4 °C overnight and then rotated with Dynabeads Protein G (Thermo Fisher Scientific) at 4 °C for 1.5 h. The beads were washed three times with wash buffer (1:1 mixture of whole-cell lysate buffer and dilution buffer). .. The recombinant human GST-TAB1-C protein (WT and Y481F) derived from Escherichia coli ( ) was reacted with the recombinant human active GST-Src kinase derived from insect cells (Carna Bioscience) at 30 °C for 30 min in 30 μl of reaction buffer containing 20 mM HEPES (pH 7.6), 20 mM MgCl 2 , 0.2 mM ATP, 2 mM DTT, 20 mM β-glycerophosphate, and 0.1 mM sodium orthovanadate. .. Recombinant human 6 × His-Src protein derived from E. coli (RayBiotech, GA) was reacted with the recombinant human active GST-p38α kinase derived from E. coli (Carna Bioscience).

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1.
    Article Snippet: After centrifugation, lysates were incubated with the anti-EGFP antibody at 4°C overnight and then rotated with Dynabeads Protein G (Thermo Fisher Scientific) at 4°C for 1.5 h. The beads were washed three times with wash buffer (1:1 mixture of whole-cell lysate buffer and dilution buffer). .. In vitro kinase assay The recombinant human GST-TAB1-C protein (WT and Y481F) derived from Escherichia coli (34) was reacted with the recombinant human active GST-Src kinase derived from insect cells (Carna Bioscience, Japan) at 30°C for 30 min in 30 μl of reaction buffer containing 20 mM HEPES (pH 7.6), 20 mM MgCl2, 0.2 mM ATP, 2 mM DTT, 20 mM β-glycerophosphate, and 0.1 mM sodium orthovanadate. .. Recombinant human 6×His-Src protein derived from E. coli (RayBiotech, GA) was reacted with the recombinant human active GST-p38α kinase derived from E. coli (Carna Bioscience, Japan).

    Concentration Assay:

    Article Title: Discovery of Pyridine-2-Carboxamides Derivatives as Potent and Selective HPK1 Inhibitors for the Treatment of Cancer
    Article Snippet: .. The recombinant human SRC protein (final concentration 0.3nM, purchased from Carna Biosciences, Cat No, 08-173) was preincubated with serially diluted compounds (10000-0.50805 nM, 3-fold increments) in a reaction buffer [50 mM HEPES, pH 7.5; 10 mM MgCl2; 2 mM DTT; 0.01% Triton X-100; 0.01% BSA] for 10 min at room temperature. ..

    Kinase Assay:

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1.
    Article Snippet: After centrifugation, lysates were incubated with the anti-EGFP antibody at 4°C overnight and then rotated with Dynabeads Protein G (Thermo Fisher Scientific) at 4°C for 1.5 h. The beads were washed three times with wash buffer (1:1 mixture of whole-cell lysate buffer and dilution buffer). .. In vitro kinase assay The recombinant human GST-TAB1-C protein (WT and Y481F) derived from Escherichia coli (34) was reacted with the recombinant human active GST-Src kinase derived from insect cells (Carna Bioscience, Japan) at 30°C for 30 min in 30 μl of reaction buffer containing 20 mM HEPES (pH 7.6), 20 mM MgCl2, 0.2 mM ATP, 2 mM DTT, 20 mM β-glycerophosphate, and 0.1 mM sodium orthovanadate. .. Recombinant human 6×His-Src protein derived from E. coli (RayBiotech, GA) was reacted with the recombinant human active GST-p38α kinase derived from E. coli (Carna Bioscience, Japan).



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    Image Search Results


    Cav1 tyrosine (Y)-14 phosphorylation induced by APC–PAR1 is mediated by c-Src. A – C, endothelial EA.hy926 cells were pretreated with dasatinib or DMSO prior to addition of APC. Cell lysates were then immunoblotted to detect Cav1 Y14 and c-Src Y416 phosphorylation as indicated. D – G, endothelial cells transfected with nonspecific (NS) or c-Src-specific siRNA were treated with or without APC, lysed, and immunoblotted as indicated. β-tubulin and GAPDH were used as loading controls. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. ( B ) ∗ p = 0.0136; ( C ) ∗∗ p = 0.0038; ( F ) ∗∗∗ p = 0.0003; and ( G ) ∗ p = 0.0125. Student's unpaired t test, ( E ) ∗∗∗ p = 0.0002. APC, activated protein C; Cav1, caveolin-1; DMSO, dimethyl sulfoxide; PAR1, protease-activated receptor 1.

    Journal: The Journal of Biological Chemistry

    Article Title: Activated protein C drives β-arrestin-2- and c-Src-dependent phosphorylation of Cav1 and modulates Cav1 association with PAR1 and GRK5

    doi: 10.1016/j.jbc.2026.111190

    Figure Lengend Snippet: Cav1 tyrosine (Y)-14 phosphorylation induced by APC–PAR1 is mediated by c-Src. A – C, endothelial EA.hy926 cells were pretreated with dasatinib or DMSO prior to addition of APC. Cell lysates were then immunoblotted to detect Cav1 Y14 and c-Src Y416 phosphorylation as indicated. D – G, endothelial cells transfected with nonspecific (NS) or c-Src-specific siRNA were treated with or without APC, lysed, and immunoblotted as indicated. β-tubulin and GAPDH were used as loading controls. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. ( B ) ∗ p = 0.0136; ( C ) ∗∗ p = 0.0038; ( F ) ∗∗∗ p = 0.0003; and ( G ) ∗ p = 0.0125. Student's unpaired t test, ( E ) ∗∗∗ p = 0.0002. APC, activated protein C; Cav1, caveolin-1; DMSO, dimethyl sulfoxide; PAR1, protease-activated receptor 1.

    Article Snippet: In this study, the following antibodies were used: mouse anti–PAR1 WEDE (Beckman Coulter, #IM2584), anti-Cav1 (CST, #3267S and BD, #610060), anti-Cav1 Y14 phospho antibody (CST, #3251), anti-βarr2 (Abcam, #ab54790), GAPDH (GeneTex, #GT239), c-Src Y416 (CST, #2101), anti-c-Src (CST, #2109), GRK5 (Santa Cruz, #sc-518005), GRK5 polyclonal antibody (Invitrogen, #PA5-96262) anti-GRK4-6 (Millipore, #05-466), anti-HA (CST, #3724S), anti-rabbit IgG (CST, #2729), anti-β-Tubulin (CST, #86298), anti–early endosome antigen-1 (BD Biosciences, #610457), anti-Vinculin (Sigma, #V9131), and anti-HA conjugated to horseradish peroxidase (Roche, #11667475001).

    Techniques: Phospho-proteomics, Transfection, Control

    βarr2 is required for APC-stimulated c-Src tyrosine (Y)-416 phosphorylation and Cav1 Y14 phosphorylation. Endothelial EA.hy926 cells were transfected with nonspecific (NS) or βarr2-specific siRNA, treated with APC, and c-Src Y416 phosphorylation ( A and B ) and Cav1 Y14 phosphorylation ( C and D ) were detected by immunoblotting as indicated. GAPDH was used as a loading control. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. B, NS siRNA with and without APC, ∗∗ p = 0.0097; ns = not significant. D, NS siRNA with and without APC ∗∗∗ p = 0.0009; βarr2 siRNA with and without APC, ∗∗ p = 0.0061. APC, activated protein C; βarr2, β-arrestin-2; Cav1, caveolin-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Activated protein C drives β-arrestin-2- and c-Src-dependent phosphorylation of Cav1 and modulates Cav1 association with PAR1 and GRK5

    doi: 10.1016/j.jbc.2026.111190

    Figure Lengend Snippet: βarr2 is required for APC-stimulated c-Src tyrosine (Y)-416 phosphorylation and Cav1 Y14 phosphorylation. Endothelial EA.hy926 cells were transfected with nonspecific (NS) or βarr2-specific siRNA, treated with APC, and c-Src Y416 phosphorylation ( A and B ) and Cav1 Y14 phosphorylation ( C and D ) were detected by immunoblotting as indicated. GAPDH was used as a loading control. The data were quantified (mean ± SD) from four independent biological replicates and expressed as the fraction relative to the untreated control and analyzed by two-way ANOVA followed by Šídák's multiple comparisons test. B, NS siRNA with and without APC, ∗∗ p = 0.0097; ns = not significant. D, NS siRNA with and without APC ∗∗∗ p = 0.0009; βarr2 siRNA with and without APC, ∗∗ p = 0.0061. APC, activated protein C; βarr2, β-arrestin-2; Cav1, caveolin-1.

    Article Snippet: In this study, the following antibodies were used: mouse anti–PAR1 WEDE (Beckman Coulter, #IM2584), anti-Cav1 (CST, #3267S and BD, #610060), anti-Cav1 Y14 phospho antibody (CST, #3251), anti-βarr2 (Abcam, #ab54790), GAPDH (GeneTex, #GT239), c-Src Y416 (CST, #2101), anti-c-Src (CST, #2109), GRK5 (Santa Cruz, #sc-518005), GRK5 polyclonal antibody (Invitrogen, #PA5-96262) anti-GRK4-6 (Millipore, #05-466), anti-HA (CST, #3724S), anti-rabbit IgG (CST, #2729), anti-β-Tubulin (CST, #86298), anti–early endosome antigen-1 (BD Biosciences, #610457), anti-Vinculin (Sigma, #V9131), and anti-HA conjugated to horseradish peroxidase (Roche, #11667475001).

    Techniques: Phospho-proteomics, Transfection, Western Blot, Control

    Model of PAR1–GRK5–βarr2–c-Src regulation by Cav1. A substantial population of PAR1–Cav1 and GRK5–Cav1 complexes coexist at the plasma membrane under basal conditions. APC bound to EPCR cleaves and activates PAR1, resulting in GRK5-dependent phosphorylation of the receptor C-terminal tail within 30 min. This may occur during a time when GRK5 dissociates from Cav1. Next, APC-activated and phosphorylated PAR1 recruits βarr2, which promotes activation of c-Src Y416 phosphorylation and c-Src-dependent Cav1 Y14 phosphorylation, a process that occurs after 30 min and is sustained through 90 min. At 30 min, GRK5–Cav1 may reassociate, whereas activated PAR1 and Y14 phosphorylated Cav1 remain dissociated through the 90 min interval. APC, activated protein C; βarr2, βarr2; Cav1, caveolin-1; EPCR, endothelial protein C receptor; GRK5, GPCR kinase 5; PAR1, protease-activated receptor-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Activated protein C drives β-arrestin-2- and c-Src-dependent phosphorylation of Cav1 and modulates Cav1 association with PAR1 and GRK5

    doi: 10.1016/j.jbc.2026.111190

    Figure Lengend Snippet: Model of PAR1–GRK5–βarr2–c-Src regulation by Cav1. A substantial population of PAR1–Cav1 and GRK5–Cav1 complexes coexist at the plasma membrane under basal conditions. APC bound to EPCR cleaves and activates PAR1, resulting in GRK5-dependent phosphorylation of the receptor C-terminal tail within 30 min. This may occur during a time when GRK5 dissociates from Cav1. Next, APC-activated and phosphorylated PAR1 recruits βarr2, which promotes activation of c-Src Y416 phosphorylation and c-Src-dependent Cav1 Y14 phosphorylation, a process that occurs after 30 min and is sustained through 90 min. At 30 min, GRK5–Cav1 may reassociate, whereas activated PAR1 and Y14 phosphorylated Cav1 remain dissociated through the 90 min interval. APC, activated protein C; βarr2, βarr2; Cav1, caveolin-1; EPCR, endothelial protein C receptor; GRK5, GPCR kinase 5; PAR1, protease-activated receptor-1.

    Article Snippet: In this study, the following antibodies were used: mouse anti–PAR1 WEDE (Beckman Coulter, #IM2584), anti-Cav1 (CST, #3267S and BD, #610060), anti-Cav1 Y14 phospho antibody (CST, #3251), anti-βarr2 (Abcam, #ab54790), GAPDH (GeneTex, #GT239), c-Src Y416 (CST, #2101), anti-c-Src (CST, #2109), GRK5 (Santa Cruz, #sc-518005), GRK5 polyclonal antibody (Invitrogen, #PA5-96262) anti-GRK4-6 (Millipore, #05-466), anti-HA (CST, #3724S), anti-rabbit IgG (CST, #2729), anti-β-Tubulin (CST, #86298), anti–early endosome antigen-1 (BD Biosciences, #610457), anti-Vinculin (Sigma, #V9131), and anti-HA conjugated to horseradish peroxidase (Roche, #11667475001).

    Techniques: Clinical Proteomics, Membrane, Phospho-proteomics, Activation Assay

    Experimental validation. ( A , C , E , G , I and K ) Relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via RT-qPCR. ( B , D , F , H , J and L ) Typical pictures and scores of the relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via IHC; 20×, Scale bar: 100 μm; 40×, Scale bar: 50 μm. ** P < 0.01, and *** P < 0.001

    Journal: BMC Pharmacology & Toxicology

    Article Title: Investigating the mechanisms by which bisphenol A affects osteoarthritis through a novel network toxicology framework and experimental validation

    doi: 10.1186/s40360-026-01108-0

    Figure Lengend Snippet: Experimental validation. ( A , C , E , G , I and K ) Relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via RT-qPCR. ( B , D , F , H , J and L ) Typical pictures and scores of the relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via IHC; 20×, Scale bar: 100 μm; 40×, Scale bar: 50 μm. ** P < 0.01, and *** P < 0.001

    Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies (all from Proteintech, diluted in PBS): SRC (1:200, 60315-1-Ig), ESR1 (1:200, 21244-1-AP), EGFR (1:1000, 18986-1-AP), PTGS2 (1:1000, 66351-1-Ig), PPARG (1:400, 16643-1-AP), and HSP90AA1 (1:400000, 13171-1-AP).

    Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR