iscripttm cdna synthesis kit (Bio-Rad)
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iscripttm cdna synthesis kit
Iscripttm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+kit/IScript+C+DNA+Synthesis+Kit/pm42886552-352-327-331
Average 99 stars, based on 6678 article reviews
Iscripttm Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+kit/IScript+C+DNA+Synthesis+Kit/pm42886552-352-327-331
Average 99 stars, based on 6678 article reviews
iscripttm cdna synthesis kit - by Bioz Stars,
2026-10
99/100 stars
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Synthesized:Article Title: Influence of exogenous glucagon-like peptide 2 on mechanisms of enteric neurotransmitter-mediated vasorelaxation in the isolated bovine mesenteric artery and vein. Article Snippet: The RNA integrity (IQ = 7.96 ± 0.627) was measured using the Qubit RNA Integrity and Quality Assay Kit (Q33221; ThermoFisher Scientific, Inc.) with a Qubit fluorometer (version 4.0). .. Complementary DNA was synthesized from 750 ng of RNA from each sample using the Article Title: Myofibroblast Derived Transglutaminase 2 Activity is Upregulated in Stricturing Crohn's Disease and its Inhibition Exerts Anti-Fibrotic Properties. Article Snippet: .. Reverse transcription and qPCR was performed on Article Title: Macrophage-Fibroblast Crosstalk Governs Tumor Mechanics, as Revealed by Spatiotemporal Force Mapping in 3D Spheroids. Article Snippet: The otal RNA was isolated using the GenElute Mammalian Total NA Miniprep Kit (Sigma-Aldrich) and the RNA amount was easured by a Nanodrop ND-1000 Spectrophotometer (Therofisher Scientific). .. Next, cDNA was synthesized with cDNA Synthesis:Article Title: Influence of exogenous glucagon-like peptide 2 on mechanisms of enteric neurotransmitter-mediated vasorelaxation in the isolated bovine mesenteric artery and vein. Article Snippet: The RNA integrity (IQ = 7.96 ± 0.627) was measured using the Qubit RNA Integrity and Quality Assay Kit (Q33221; ThermoFisher Scientific, Inc.) with a Qubit fluorometer (version 4.0). .. Complementary DNA was synthesized from 750 ng of RNA from each sample using the Article Title: Lymph node contraction links sex-biased naive CD8 + T cell decline to compromised antigen recognition during middle age. Article Snippet: mRNA was extracted using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. RNA reverse transcription was performed using an Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells. Article Snippet: Trypsin–phosphate versene glucose (TPVG) solution (HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. TCL031), phosphate-buffered saline (PBS; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. ML023), and bovine serum albumin Fraction V (BSA; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. MB08353) were used for routine cell culture and experimental procedures. .. MTT (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 21795), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; MedChemEx- https://doi.org/10.3390/cells15191826 press, Monmouth Junction, NJ, USA; Cat. No. HYD0940), JC-1 (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 15003), TriXtract (G-Biosciences, St. Louis, MO, USA; Cat. No. 786652), Ethanol (Empure, Sigma -Aldrich/Merk, 100986, Darmstadt, Germany), Article Title: Myofibroblast Derived Transglutaminase 2 Activity is Upregulated in Stricturing Crohn's Disease and its Inhibition Exerts Anti-Fibrotic Properties. Article Snippet: .. Reverse transcription and qPCR was performed on Modification:Article Title: Influence of exogenous glucagon-like peptide 2 on mechanisms of enteric neurotransmitter-mediated vasorelaxation in the isolated bovine mesenteric artery and vein. Article Snippet: The RNA integrity (IQ = 7.96 ± 0.627) was measured using the Qubit RNA Integrity and Quality Assay Kit (Q33221; ThermoFisher Scientific, Inc.) with a Qubit fluorometer (version 4.0). .. Complementary DNA was synthesized from 750 ng of RNA from each sample using the Virus:Article Title: Influence of exogenous glucagon-like peptide 2 on mechanisms of enteric neurotransmitter-mediated vasorelaxation in the isolated bovine mesenteric artery and vein. Article Snippet: The RNA integrity (IQ = 7.96 ± 0.627) was measured using the Qubit RNA Integrity and Quality Assay Kit (Q33221; ThermoFisher Scientific, Inc.) with a Qubit fluorometer (version 4.0). .. Complementary DNA was synthesized from 750 ng of RNA from each sample using the Reverse Transcription:Article Title: Influence of exogenous glucagon-like peptide 2 on mechanisms of enteric neurotransmitter-mediated vasorelaxation in the isolated bovine mesenteric artery and vein. Article Snippet: The RNA integrity (IQ = 7.96 ± 0.627) was measured using the Qubit RNA Integrity and Quality Assay Kit (Q33221; ThermoFisher Scientific, Inc.) with a Qubit fluorometer (version 4.0). .. Complementary DNA was synthesized from 750 ng of RNA from each sample using the Article Title: Biopsy-Based Molecular Signatures Predicting Therapeutic Response in Ulcerative Colitis. Article Snippet: The RNA concentration was determined with the NanoDrop ND-2000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Total RNA (1 μg) was reverse transcribed using an Article Title: Lymph node contraction links sex-biased naive CD8 + T cell decline to compromised antigen recognition during middle age. Article Snippet: mRNA was extracted using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. RNA reverse transcription was performed using an Article Title: Myofibroblast Derived Transglutaminase 2 Activity is Upregulated in Stricturing Crohn's Disease and its Inhibition Exerts Anti-Fibrotic Properties. Article Snippet: .. Reverse transcription and qPCR was performed on DNA Synthesis:Article Title: Biopsy-Based Molecular Signatures Predicting Therapeutic Response in Ulcerative Colitis. Article Snippet: The RNA concentration was determined with the NanoDrop ND-2000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Total RNA (1 μg) was reverse transcribed using an Article Title: Macrophage-Fibroblast Crosstalk Governs Tumor Mechanics, as Revealed by Spatiotemporal Force Mapping in 3D Spheroids. Article Snippet: The otal RNA was isolated using the GenElute Mammalian Total NA Miniprep Kit (Sigma-Aldrich) and the RNA amount was easured by a Nanodrop ND-1000 Spectrophotometer (Therofisher Scientific). .. Next, cDNA was synthesized with Chloramphenicol Acetyltransferase Assay:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 Recombinant:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 Generated:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 Software:Article Title: Central relaxin-3/RXFP3 signaling potently drives orexigenic behaviors and attenuates cisplatin-induced anorexia and body weight loss. Article Snippet: Adult wildtype (∼8 weeks old) maleSprague-Dawley rats (Charles River, RRID:RGD_734476) weighing ∼250–275 upon arrival were individually housed under a 12h-light:12h-dark cycle in a temperature and humidity-controlled satellite vivarium and had ad libitum access to water and chow (5001, LabDiet; Calories provided by protein 28.9%, fat 13.6%, carbohydrate 57.5%; 3.35 kcal/g) or a 60% high fat diet (HFD; D12492, Research diets; Calories provided by protein 26.2%, fat 34.9%, carbohydrate 26.3%; 5.24 kcal/g) and when indicated had ad libitum access to kaolin pellets (Research Diets, K50001) to assess pica behavior. .. Rats were exposed to kaolin REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Mouse brain (Rxfp3 fl/fl hsyn-Cre+) In house (University of Pennsylvania) N/A Mouse brain (Rxfp3 fl/fl hsyn-Cre-) In house (University of Pennsylvania) CAT JAX:028543 Mouse brain (hSyn-Cre+) In house (University of Pennsylvania) CAT# JAX:003966 Chemicals, peptides, and recombinant proteins Relaxin-3 Phoenix Pharmaceuticals CAT# 035–36 Cisplatin Sigma Aldrich CAT# 15663–27-1 GDF-15 Abcam ab302451 LiCl DMSO Honeywell CAT# 34869-1L Artificial cerebrospinal fluid (aCSF) Havard Appartus CAT# 59–7316 Angiotensin II (Ang II) Bachem Product #4006473 Critical commercial assays Fast Advanced Master Mix Applied Biosystems CAT# 4444554 other:Article Title: Lymphangiogenesis driven by VEGF-C reshapes tumor immune landscape and enables tumor eradication by viral immunotherapy in mouse melanoma models. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse CD8a Brilliant Violet 510TM, clone 53-6.7 Biolegend Cat# 100751; RRID: AB_2561389 Anti-mouse CD80 V450, clone 16-10A1 BD Biosciences Cat# 560523; RRID: AB_1727515 Anti-mouse CD83 Brilliant Violet 650TM, clone Michel-19 Biolegend Cat# 121515; RRID: AB_11203713 Anti-mouse CD86 FITC, clone GL-1 Biolegend Cat# 105005; RRID: AB_313148 Anti-mouse CD152 APC-R700, clone UC10-4F10-11 BD Biosciences Cat# 565778; RRID: AB_2739350 Anti-mouse CD183 BV650,clone CXCR3-173 BD Biosciences Cat# 740630; RRID: AB_2740325 Anti-mouse F4/80 Brilliant Violet 421TM, clone BM8 Biolegend Cat# 123131; RRID: AB_10901171 Anti-mouse Foxp3 V450, clone MF23 BD Biosciences Cat# 561293; RRID: AB_10611728 Anti-human/mouse Granzyme B FITC, clone GB11 Biolegend Cat# 515403; RRID: AB_2114575 Anti-mouse IFN-γ Brilliant Violet 711TM, clone XMG1.2 Biolegend Cat# 505835; RRID: AB_11219588 Anti-mouse KLRG1 V450, clone 2F1 BD Biosciences Cat# 561625; RRID: AB_10892825 Anti-mouse Ly-6C Alexa Fluor 700, clone HK1.4 Biolegend Cat# 128023; RRID: AB_10640119 Anti-mouse Ly-6G PE/Cy7, clone 1A8 Biolegend Cat# 127617; RRID: AB_1877262 Anti-mouse MHCII Brilliant Violet 785TM, clone M5/114.15.2 Biolegend Cat# 107645; RRID: AB_2565977 Anti-mouse NK-1.1 Brilliant Violet 650TM, clone PK136 Biolegend Cat# 108735; RRID: AB_11147949 Anti-mouse CD274 (PD-L1) BV711, clone 10F.9G2 BD Biosciences Cat# 568562; RRID: AB_3684373 Anti-mouse CD279 (PD-1) Brilliant Violet 605TM, clone 29F.1A12 Biolegend Cat# 135219; RRID: AB_11125371 Anti-mouse TNF BB700, clone MP6-XT22 BD Biosciences Cat# 566510; RRID: AB_2869775 Anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2354; RRID: AB_628490 Anti-mouse CD11c PerCP-Cy5.5 Biolegend Cat# 117328; RRID: AB_2129641 Anti-mouse CD11b PE BD Biosciences Cat# 553311; RRID: AB_394775 Anti-mouse NKp46 PE-Dazzle594 Biolegend Cat# 137630; RRID: AB_2616666 Anti-mouse Ly6G PE-Cy7 Biolegend Cat# 127618; RRID: AB_1877261 Anti-mouse MHC-II eFluor450 Thermo Fisher Scientific Cat# 48–5321-82; RRID: AB_1272204 Anti-mouse CD4 BV510 BD Biosciences Cat# 563106; RRID: AB_2687550 Anti-mouse Ly6C BV605 BD Biosciences Cat# 563011; RRID: AB_2737949 Anti-mouse CD3 BV650 BD Biosciences Cat# 564378; RRID: AB_2738779 Anti-mouse CD45 BV750 BD Biosciences Cat# 746947; RRID: AB_2871734 Bacterial and virus strains NDV LaSota-L289A Adolfo Garcia-Sastre Lab N/A APMV-4 Duck/Hong Kong/D3/1975 Adolfo Garcia-Sastre Lab N/A Critical commercial assays Amicon Ultra-15 Centrifugal Filter Unit Sigma Millipore Cat# UFC900324 MTT Assay:Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells. Article Snippet: Trypsin–phosphate versene glucose (TPVG) solution (HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. TCL031), phosphate-buffered saline (PBS; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. ML023), and bovine serum albumin Fraction V (BSA; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. MB08353) were used for routine cell culture and experimental procedures. .. MTT (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 21795), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; MedChemEx- https://doi.org/10.3390/cells15191826 press, Monmouth Junction, NJ, USA; Cat. No. HYD0940), JC-1 (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 15003), TriXtract (G-Biosciences, St. Louis, MO, USA; Cat. No. 786652), Ethanol (Empure, Sigma -Aldrich/Merk, 100986, Darmstadt, Germany), SYBR Green Assay:Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells. Article Snippet: Trypsin–phosphate versene glucose (TPVG) solution (HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. TCL031), phosphate-buffered saline (PBS; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. ML023), and bovine serum albumin Fraction V (BSA; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. MB08353) were used for routine cell culture and experimental procedures. .. MTT (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 21795), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; MedChemEx- https://doi.org/10.3390/cells15191826 press, Monmouth Junction, NJ, USA; Cat. No. HYD0940), JC-1 (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 15003), TriXtract (G-Biosciences, St. Louis, MO, USA; Cat. No. 786652), Ethanol (Empure, Sigma -Aldrich/Merk, 100986, Darmstadt, Germany), Article Title: Myofibroblast Derived Transglutaminase 2 Activity is Upregulated in Stricturing Crohn's Disease and its Inhibition Exerts Anti-Fibrotic Properties. Article Snippet: .. Reverse transcription and qPCR was performed on ATP Assay:Article Title: Radiofrequency Radiation Alters Redox Homeostasis, ATP Status, and Apoptosis-Associated Responses in Human Ovarian Epithelial Cells. Article Snippet: Trypsin–phosphate versene glucose (TPVG) solution (HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. TCL031), phosphate-buffered saline (PBS; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. ML023), and bovine serum albumin Fraction V (BSA; HiMedia Laboratories, Thane, Maharashtra, India; Cat. No. MB08353) were used for routine cell culture and experimental procedures. .. MTT (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 21795), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA; MedChemEx- https://doi.org/10.3390/cells15191826 press, Monmouth Junction, NJ, USA; Cat. No. HYD0940), JC-1 (Cayman Chemical, Ann Arbor, MI, USA; Cat. No. 15003), TriXtract (G-Biosciences, St. Louis, MO, USA; Cat. No. 786652), Ethanol (Empure, Sigma -Aldrich/Merk, 100986, Darmstadt, Germany), Real-time Polymerase Chain Reaction:Article Title: Myofibroblast Derived Transglutaminase 2 Activity is Upregulated in Stricturing Crohn's Disease and its Inhibition Exerts Anti-Fibrotic Properties. Article Snippet: .. Reverse transcription and qPCR was performed on |