bugbuster solution (Merck KGaA)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Merck KGaA bugbuster solution
Bugbuster Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bugbuster+solution/bugbuster/pmc08588357-243-12-15
Average 90 stars, based on 1 article reviews

bugbuster solution - by Bioz Stars, 2026-10

90/100 stars

Images

Lysis:

Article Title: Small-scale fed-batch cultivations of Vibrio natriegens: overcoming challenges for early process development.
Article Snippet: .. After thawing, cell lysis was performed using BugBuster® (Merck, KGaA, Darmstadt, Germany), following the manufacturer’s instructions with the addition of Benzonase® (Sigma Aldrich, Steinheim, Germany) and lysozyme (Carl Roth, Karlsruhe, Germany). ..

Centrifugation:

Article Title: Engineered reversible inhibition of SpyCatcher reactivity enables rapid generation of bispecific antibodies
Article Snippet: .. Cells were harvested by centrifugation, frozen, and lysed with BugBuster (Merck KGaA), supplemented with 2 mg/mL lysozyme (Roche). .. Purification was performed using Ni-NTA agarose (Qiagen) as the matrix, with 20 mM sodium phosphate, pH 7.4, 500 mM sodium chloride, and 30 mM (wash) or 250 mM (elution) imidazole as buffers.

Article Title: Engineered reversible inhibition of SpyCatcher reactivity enables rapid generation of bispecific antibodies.
Article Snippet: .. Cells were harvested by centrifugation, frozen, and lysed with BugBuster (Merck KGaA), supplemented with 2mg/mL lysozyme (Roche). .. Cells were harvested by centrifugation, frozen, and lysed with BugBuster (Merck KGaA), supplemented with 2mg/mL lysozyme (Roche).

Recombinant:

Article Title: Characterization of a lipocalin-like molecule from Dermanyssus gallinae as a potential vaccine antigen.
Article Snippet: Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites of chickens that pose a significant threat to the egg-laying industry.. The emergence of acaricide-resistant PRMs raises the demand for alternative control approaches such as vaccination.. However, several vaccine antigens have failed to suppress the growth of PRM populations in field trials due to difficulties in maintaining antibody levels.

Article Title: In Silico and In Vitro Studies of the Approved Antibiotic Ceftaroline Fosamil and Its Metabolites as Inhibitors of SARS-CoV-2 Replication
Article Snippet: .. To purify the recombinant enzymes, cells were harvested, lysed with BugBuster ® (Merck Millipore, Burlington, MA, USA), and centrifuged at 16,000× g for 20 min at 4 °C. .. The supernatant was submitted to affinity chromatography on a nickel–agarose resin (Sigma, Burlington, MA, USA).

Article Title: Seminal vesicle protein ca CA12 in Corydoras aeneus inhibits sperm motility for sperm drinking
Article Snippet: .. The recombinant proteins were extracted by BugBuster (Merck, Darmstadt, Germany) and purified by Glutathione Sepharose TM 4B (GE Healthcare, Tokyo, Japan) and HIS-Select Nickel Affinity Gel (Sigma-Aldrich, St Louis, MO, USA). .. The C-terminal repeat sequence of ca CA12, TAYPTIQPFAKLPIQP, was synthesized by a peptide synthesis service (GenScript Japan KK, Tokyo, Japan).

Chromatography:

Article Title: Biosynthesis of novel cannabigerolic acid derivatives by engineering the substrate specificity of aromatic prenyltransferase
Article Snippet: .. The harvested cells were either directly applied to whole-cell bioconversion for the biosynthesis of CBGA derivatives or lysed by Bugbuster ® (Merck Millipore, Billerica, United States), centrifuged (8,000 g, 4°C, 20 min) for cell debris removal, subjected to Ni-affinity chromatography (Qiagen, Hilden, Germany) for elution with elution buffer (50 mM Tris-HCl, 300 mM NaCl, 250 mM imidazole), and concentrated using Amicon Ultra 15 mL centrifugal filters with a 10 kDa pore size. ..

Pore Size:

Purification:

Protein Extraction:

Article Title: Stable Platform for Mevalonate Bioproduction from CO 2
Article Snippet: Preparation of protein samples for SDS-PAGE analysis The pre- and post-induction cell pellets prepared as described in paragraph 2.6 were lysed using the BugBuster® protein extraction reagent (Merck-Millipore). .. The protein extraction mix was prepared by diluting BugBuster® (1:10); Protease Inhibitor Cocktail Set VII (1:50); rLysozymeTM (1:1000) and benzonase (1:10000) in appropriate volumes of PBS (all these reagents were purchased from Merck-Millipore). ..

Review