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Merck & Co bugbuster solution
Bugbuster Solution, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bugbuster+solution/bugbust/pmc11735211-151-11-13
Average 90 stars, based on 1 article reviews
bugbuster solution - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Centrifugation:

Article Title: Polyelectrolyte mannan from diatoms reshapes sunlit ocean microbiome
Article Snippet: .. For preliminary enzyme screening experiments one spatula of cell pellet were lyzed using BugBuster (Merck) according to manufacturer’s instructions and cell debris was removed by centrifugation. ..

Article Title: T cell receptor for identifying AFP antigen
Article Snippet: The cell pellets were harvested by centrifugation at 5000 rpm for 15 mins, and the cell pellets were lysed with Bugbuster Master Mix (Merck). .. The inclusion bodies were recovered by centrifugation at 6000 rpm for 15 min, followed by washing with Bugbuster (Merck) to remove cell debris and membrane fraction. ..

Membrane:

Article Title: T cell receptor for identifying AFP antigen
Article Snippet: The cell pellets were harvested by centrifugation at 5000 rpm for 15 mins, and the cell pellets were lysed with Bugbuster Master Mix (Merck). .. The inclusion bodies were recovered by centrifugation at 6000 rpm for 15 min, followed by washing with Bugbuster (Merck) to remove cell debris and membrane fraction. ..

other:


Lysis:

Article Title: Shigella flexneri evades LPS ubiquitylation through IpaH1.4-mediated degradation of RNF213
Article Snippet: .. The supernatant, containing the clarified host cell lysate, was saved, and the bacterial pellet was washed once with PBS, followed by bacterial lysis in 35 μL BugBuster (Merck) including 2 mM iodoacetamide for 5 minutes at room temperature with occasional vortexing. ..

Article Title: Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity.
Article Snippet: Bacterial isolation prior to immunoblotting To analyze ubiquitylation of bacteria as previously described,5 infected cells, at 4 h post-invasion for Salmonella and 24 h post-invasion for Burkholderia, were lysed in 5 mL of cold lysis buffer (1% Triton X-100, 30 mM HEPES (pH 7.5), 100 mM NaCl, 10 mM MgCl2, 10 mM Iodoacetamide and complete protease inhibitor cocktail (Roche)) for 5 min. After a sample was collected for analysis by immunoblot with anti-tubulin (or anti-GFP antibodies when required), lysates were centrifuged at 300 3 g for 5 min. .. The bacteria-containing supernatant was collected and centrifuged at 16,100 3 g for 10 min at 4 C. The bacterial pellets were washed with lysis buffer and lysed in 50 mL of BugBuster (Merck) supplemented with 10 mM Iodoacetamide and protease inhibitor cocktail. ..

Incubation:

Article Title: Computational scoring and experimental evaluation of enzymes generated by neural networks.
Article Snippet: .. Cell pellets were suspended in 200 μl BugBuster reagent (cat. no. 70584, Merck) supplemented with 1 μl 2,000 U ml−1 DNase I (cat. no. 79254, Qiagen) and incubated at 37 °C with shaking at 200 rpm for 30 min. After incubation, 10-μl mixtures were aliquoted and kept in −20 °C as the total protein (T) sample for gel electrophoresis. ..

Nucleic Acid Electrophoresis:

Article Title: Computational scoring and experimental evaluation of enzymes generated by neural networks.
Article Snippet: .. Cell pellets were suspended in 200 μl BugBuster reagent (cat. no. 70584, Merck) supplemented with 1 μl 2,000 U ml−1 DNase I (cat. no. 79254, Qiagen) and incubated at 37 °C with shaking at 200 rpm for 30 min. After incubation, 10-μl mixtures were aliquoted and kept in −20 °C as the total protein (T) sample for gel electrophoresis. ..

Recombinant:

Article Title: Potential of histamine release factor for the utilization as a universal vaccine antigen against poultry red mites, tropical fowl mites, and northern fowl mites
Article Snippet: .. Recombinant proteins were extracted from the inclusion body fractions using the BugBuster solution (Merck). .. The proteins were solubilized in a buffer containing 0.3% N-lauroylsarcosine and 50 mM N-cyclohexyl-3-aminopropanesulfonic acid (CAPS; Merck) (pH 11.0).

Bacteria:

Article Title: Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity.
Article Snippet: Bacterial isolation prior to immunoblotting To analyze ubiquitylation of bacteria as previously described,5 infected cells, at 4 h post-invasion for Salmonella and 24 h post-invasion for Burkholderia, were lysed in 5 mL of cold lysis buffer (1% Triton X-100, 30 mM HEPES (pH 7.5), 100 mM NaCl, 10 mM MgCl2, 10 mM Iodoacetamide and complete protease inhibitor cocktail (Roche)) for 5 min. After a sample was collected for analysis by immunoblot with anti-tubulin (or anti-GFP antibodies when required), lysates were centrifuged at 300 3 g for 5 min. .. The bacteria-containing supernatant was collected and centrifuged at 16,100 3 g for 10 min at 4 C. The bacterial pellets were washed with lysis buffer and lysed in 50 mL of BugBuster (Merck) supplemented with 10 mM Iodoacetamide and protease inhibitor cocktail. ..

Protease Inhibitor:

Article Title: Bacterial esterases reverse lipopolysaccharide ubiquitylation to block host immunity.
Article Snippet: Bacterial isolation prior to immunoblotting To analyze ubiquitylation of bacteria as previously described,5 infected cells, at 4 h post-invasion for Salmonella and 24 h post-invasion for Burkholderia, were lysed in 5 mL of cold lysis buffer (1% Triton X-100, 30 mM HEPES (pH 7.5), 100 mM NaCl, 10 mM MgCl2, 10 mM Iodoacetamide and complete protease inhibitor cocktail (Roche)) for 5 min. After a sample was collected for analysis by immunoblot with anti-tubulin (or anti-GFP antibodies when required), lysates were centrifuged at 300 3 g for 5 min. .. The bacteria-containing supernatant was collected and centrifuged at 16,100 3 g for 10 min at 4 C. The bacterial pellets were washed with lysis buffer and lysed in 50 mL of BugBuster (Merck) supplemented with 10 mM Iodoacetamide and protease inhibitor cocktail. ..



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