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mouse tissue sections  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc mouse tissue sections
    Mouse Tissue Sections, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brain+tissue+section/Mouse+Brain+Tissue+Control+Extracts/pm41634038-264-5-27
    Average 94 stars, based on 91 article reviews
    mouse tissue sections - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells.
    Article Snippet: .. The sample was incubated at X-CLARITYTM Mounting Solution for 1 h at room temperature, followed by a rinse with fresh X-CLARITYTM Mounting Solution and additional incubation for 1–2 h. Tissue preparation, immunostaining, and microscopy of brain & spleen tissue Mouse brain and spleen coronal Sect. (4 μm thickness) were prepared with H&E staining; F4/80 mAb (Cell Signaling Technologies, Rabbit mAb #70076) 1:250 to identify macrophages; CD4 mAb (Cell Signaling Technologies, Rabbit mAb #25229) 1:100 to identify CD4 + T-Cells; CD8a mAb (Cell Signaling Technologies, Rabbit mAb # 98941) 1:400 to identify CD8 + T-Cells; and Granzyme B mAb (Cell Signaling Technologies, Rabbit mAb # 44153) 1:100 to identify activated T cells and natural killer cells. ..

    Immunostaining:

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells.
    Article Snippet: .. The sample was incubated at X-CLARITYTM Mounting Solution for 1 h at room temperature, followed by a rinse with fresh X-CLARITYTM Mounting Solution and additional incubation for 1–2 h. Tissue preparation, immunostaining, and microscopy of brain & spleen tissue Mouse brain and spleen coronal Sect. (4 μm thickness) were prepared with H&E staining; F4/80 mAb (Cell Signaling Technologies, Rabbit mAb #70076) 1:250 to identify macrophages; CD4 mAb (Cell Signaling Technologies, Rabbit mAb #25229) 1:100 to identify CD4 + T-Cells; CD8a mAb (Cell Signaling Technologies, Rabbit mAb # 98941) 1:400 to identify CD8 + T-Cells; and Granzyme B mAb (Cell Signaling Technologies, Rabbit mAb # 44153) 1:100 to identify activated T cells and natural killer cells. ..

    Microscopy:

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells.
    Article Snippet: .. The sample was incubated at X-CLARITYTM Mounting Solution for 1 h at room temperature, followed by a rinse with fresh X-CLARITYTM Mounting Solution and additional incubation for 1–2 h. Tissue preparation, immunostaining, and microscopy of brain & spleen tissue Mouse brain and spleen coronal Sect. (4 μm thickness) were prepared with H&E staining; F4/80 mAb (Cell Signaling Technologies, Rabbit mAb #70076) 1:250 to identify macrophages; CD4 mAb (Cell Signaling Technologies, Rabbit mAb #25229) 1:100 to identify CD4 + T-Cells; CD8a mAb (Cell Signaling Technologies, Rabbit mAb # 98941) 1:400 to identify CD8 + T-Cells; and Granzyme B mAb (Cell Signaling Technologies, Rabbit mAb # 44153) 1:100 to identify activated T cells and natural killer cells. ..

    Staining:

    Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells.
    Article Snippet: .. The sample was incubated at X-CLARITYTM Mounting Solution for 1 h at room temperature, followed by a rinse with fresh X-CLARITYTM Mounting Solution and additional incubation for 1–2 h. Tissue preparation, immunostaining, and microscopy of brain & spleen tissue Mouse brain and spleen coronal Sect. (4 μm thickness) were prepared with H&E staining; F4/80 mAb (Cell Signaling Technologies, Rabbit mAb #70076) 1:250 to identify macrophages; CD4 mAb (Cell Signaling Technologies, Rabbit mAb #25229) 1:100 to identify CD4 + T-Cells; CD8a mAb (Cell Signaling Technologies, Rabbit mAb # 98941) 1:400 to identify CD8 + T-Cells; and Granzyme B mAb (Cell Signaling Technologies, Rabbit mAb # 44153) 1:100 to identify activated T cells and natural killer cells. ..

    Article Title: Presymptomatic pharmacological inhibition of mGluR5 improves survival in a mouse model of prion diseases.
    Article Snippet: .. For detection of glial fibrillary acidic protein (GFAP, Dako M0761), ionized calcium binding adaptor molecule 1 (IBA1, Wako Pure Chemical Industries 019-19741) in mouse brain tissue, or mGluR5 (1:200; #55920 Cell Signaling) in mouse and non-human primate brain tissue by immunohistochemical staining, a Ventana Benchmark XT autostainer was used (Ventana, Tuscon, Arizona, USA). .. Deparaffinization and antigen retrieval of brain tissue sections was performed, followed by incubation with primary antibody for 1 h. Anti-rabbit or anti-mouse Histofine Simple Stain MAX PO Universal immunoperoxidase polymer (Nichirei Biosciences, Wedel, Germany) were used as secondary antibodies.

    other:

    Article Title: Mass Spectrometry Imaging Reveals Region-Specific Lipid Alterations in the Mouse Brain in Response to Efavirenz Treatment.
    Article Snippet: Efavirenz (EFV) is a commonly used drug to treat human immunodeficiency virus infection and is known to exert adverse effects on the brain.. Although it is known that EFV is associated with abnormal plasma lipid levels, the changes in the spatial localization of individual lipid molecules in brain tissue following EFV treatment are yet to be explored.. In this study, we employed a matrix-assisted laser desorption/ionization mass spectrometry imaging approach to determine region-specific lipid alterations in mouse brains following EFV treatment.


    Binding Assay:

    Article Title: Presymptomatic pharmacological inhibition of mGluR5 improves survival in a mouse model of prion diseases.
    Article Snippet: .. For detection of glial fibrillary acidic protein (GFAP, Dako M0761), ionized calcium binding adaptor molecule 1 (IBA1, Wako Pure Chemical Industries 019-19741) in mouse brain tissue, or mGluR5 (1:200; #55920 Cell Signaling) in mouse and non-human primate brain tissue by immunohistochemical staining, a Ventana Benchmark XT autostainer was used (Ventana, Tuscon, Arizona, USA). .. Deparaffinization and antigen retrieval of brain tissue sections was performed, followed by incubation with primary antibody for 1 h. Anti-rabbit or anti-mouse Histofine Simple Stain MAX PO Universal immunoperoxidase polymer (Nichirei Biosciences, Wedel, Germany) were used as secondary antibodies.

    Immunohistochemical staining:

    Article Title: Presymptomatic pharmacological inhibition of mGluR5 improves survival in a mouse model of prion diseases.
    Article Snippet: .. For detection of glial fibrillary acidic protein (GFAP, Dako M0761), ionized calcium binding adaptor molecule 1 (IBA1, Wako Pure Chemical Industries 019-19741) in mouse brain tissue, or mGluR5 (1:200; #55920 Cell Signaling) in mouse and non-human primate brain tissue by immunohistochemical staining, a Ventana Benchmark XT autostainer was used (Ventana, Tuscon, Arizona, USA). .. Deparaffinization and antigen retrieval of brain tissue sections was performed, followed by incubation with primary antibody for 1 h. Anti-rabbit or anti-mouse Histofine Simple Stain MAX PO Universal immunoperoxidase polymer (Nichirei Biosciences, Wedel, Germany) were used as secondary antibodies.

    TUNEL Assay:

    Article Title: Neuronal protective effect of Artemisinin in ischemic stroke: Achieved by blocking lysine demethylase 1A-mediated demethylation of sphingosine kinase 2.
    Article Snippet: Artemisinin (ART), a natural product isolated from the traditional Chinese plant Artemisia annua L., has shown neuroprotective properties in addition to its well-established antimalarial activities.. This study investigates the therapeutic effect of ART in ischemic stroke (IS) and delves into its functional mechanism.. Bioinformatics analyses revealed lysine demethylase 1A (KDM1A) as a promising target of ART aberrantly overexpressed in the context of IS.



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    Cell Signaling Technology Inc rat brain tissue sections
    Electroacupuncture exerts analgesic effect by influencing miR-21-5p/Smad7, thereby further regulating the TGF-β/Smads pathway in rats with CPSP. A - B . WB expression of TGF-β1, p-Smad2 and p-Smad3 in the VPL (ventral posterolateral) region of <t>rat</t> <t>brain</t> <t>tissue</t> in different groups ( n = 3). C . Diagram of the mechanism of electroacupuncture exerts analgesic effect on CPSP
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    Electroacupuncture exerts analgesic effect by influencing miR-21-5p/Smad7, thereby further regulating the TGF-β/Smads pathway in rats with CPSP. A - B . WB expression of TGF-β1, p-Smad2 and p-Smad3 in the VPL (ventral posterolateral) region of <t>rat</t> <t>brain</t> <t>tissue</t> in different groups ( n = 3). C . Diagram of the mechanism of electroacupuncture exerts analgesic effect on CPSP
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    Image Search Results


    Electroacupuncture exerts analgesic effect by influencing miR-21-5p/Smad7, thereby further regulating the TGF-β/Smads pathway in rats with CPSP. A - B . WB expression of TGF-β1, p-Smad2 and p-Smad3 in the VPL (ventral posterolateral) region of rat brain tissue in different groups ( n = 3). C . Diagram of the mechanism of electroacupuncture exerts analgesic effect on CPSP

    Journal: The Journal of Headache and Pain

    Article Title: Electroacupuncture alleviates central post-stroke pain in rats by modulating miR-21-5p/TGF-β/Smads pathway

    doi: 10.1186/s10194-025-02247-1

    Figure Lengend Snippet: Electroacupuncture exerts analgesic effect by influencing miR-21-5p/Smad7, thereby further regulating the TGF-β/Smads pathway in rats with CPSP. A - B . WB expression of TGF-β1, p-Smad2 and p-Smad3 in the VPL (ventral posterolateral) region of rat brain tissue in different groups ( n = 3). C . Diagram of the mechanism of electroacupuncture exerts analgesic effect on CPSP

    Article Snippet: Rat brain tissue sections, previously embedded in paraffin, underwent incubation with GFAP (1:250, Abcam, UK), IBA1 (1:250, CST, USA), and Smad7 (1:250, Proteintech, China) antibodies.

    Techniques: Expressing