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the bluescript sk1 vector  (Agilent technologies)


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    Structured Review

    Agilent technologies the bluescript sk1 vector
    The Bluescript Sk1 Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript+sk1-vector/pm10828066-57-32-35
    Average 90 stars, based on 1 article reviews
    the bluescript sk1 vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Nested PCR:

    Article Title: Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase.
    Article Snippet: .. The primers for the second nested polymerase chain reaction (PCR) contained XhoI and XbaI endonuclease restriction sites, which allowed the resulting PCR product to be cloned into the corresponding sites of the Bluescript SK1 vector (Stratagene). .. The clone was sequenced using the M13-21 ABI sequencing primer (DNA technology A/S, Aarhus, Denmark).

    Polymerase Chain Reaction:

    Article Title: Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase.
    Article Snippet: .. The primers for the second nested polymerase chain reaction (PCR) contained XhoI and XbaI endonuclease restriction sites, which allowed the resulting PCR product to be cloned into the corresponding sites of the Bluescript SK1 vector (Stratagene). .. The clone was sequenced using the M13-21 ABI sequencing primer (DNA technology A/S, Aarhus, Denmark).

    Clone Assay:

    Article Title: Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase.
    Article Snippet: .. The primers for the second nested polymerase chain reaction (PCR) contained XhoI and XbaI endonuclease restriction sites, which allowed the resulting PCR product to be cloned into the corresponding sites of the Bluescript SK1 vector (Stratagene). .. The clone was sequenced using the M13-21 ABI sequencing primer (DNA technology A/S, Aarhus, Denmark).

    Article Title: Expression of the smooth muscle cell calponin gene marks the early cardiac and smooth muscle cell lineages during mouse embryogenesis.
    Article Snippet: .. These clones were ligated into the EcoRI/HindIII sites of the Bluescript SK1 vector (Stratagene; La Jolla, CA), sequenced on both strands with Sequenase II (U. S. Biochemical Corp., Cleveland, OH), and utilized for RNase protection, in situ hybridization, or genomic screening as described below. ..

    Article Title: Activation of the IL-4 STAT pathway in rheumatoid synovium.
    Article Snippet: .. In brief, cDNA fragments of IL-4 STAT (a generous gift of Dr. U. Schindler, Tularik, Inc., San Francisco, CA, (21)) were cloned into the Bluescript SK1 vector (Stratagene, La Jolla, CA). ..

    Plasmid Preparation:

    Article Title: Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase.
    Article Snippet: .. The primers for the second nested polymerase chain reaction (PCR) contained XhoI and XbaI endonuclease restriction sites, which allowed the resulting PCR product to be cloned into the corresponding sites of the Bluescript SK1 vector (Stratagene). .. The clone was sequenced using the M13-21 ABI sequencing primer (DNA technology A/S, Aarhus, Denmark).

    Article Title: Expression of the smooth muscle cell calponin gene marks the early cardiac and smooth muscle cell lineages during mouse embryogenesis.
    Article Snippet: .. These clones were ligated into the EcoRI/HindIII sites of the Bluescript SK1 vector (Stratagene; La Jolla, CA), sequenced on both strands with Sequenase II (U. S. Biochemical Corp., Cleveland, OH), and utilized for RNase protection, in situ hybridization, or genomic screening as described below. ..

    Article Title: Plasma membrane Ca2+ pump isoforms 2a and 2b are unusually responsive to calmodulin and Ca2+.
    Article Snippet: Construction of the Full-length rPMCA2b—The full-length rPMCA2b isoform in the pBR322 vector was a gift from Dr. G. Shull (University of Cincinnati). .. The full-length rPMCA2b gene was excised from the pBR322 vector with ApaI and ligated into the Bluescript SK1 vector (Stratagene) at the ApaI site. .. The full-length DNA was excised from Bluescript SK1 vector with SalI and KpnI, cloned into the expression vector pMM2 and sequenced using the Applied Biosystems Automatic Sequencer.

    Article Title: Cloning and expression throughout mouse development ofmfat1, a homologue of theDrosophila tumour suppressor genefat
    Article Snippet: Chromogenic reactions to visualise alkaline phosphatase were carried out either using NBT/BCIP or BM purple (BoehringerMannheim Biochemicals, Indianapolis, IN). .. Clone F2, 800 bp (derived from the mfat1 39 untranslated region) in Bluescript SK1 vector (Stratagene) was linearised using Bam H1. ..

    Article Title: Characterization of a vacuolar protease in Neurospora crassa and the use of gene RIPing to generate protease-deficient strains.
    Article Snippet: .. Each of these fragments was subcloned into the Bluescript SK1 vector (Stratagene, La Jolla, CA). ..

    In Situ Hybridization:

    Article Title: Expression of the smooth muscle cell calponin gene marks the early cardiac and smooth muscle cell lineages during mouse embryogenesis.
    Article Snippet: .. These clones were ligated into the EcoRI/HindIII sites of the Bluescript SK1 vector (Stratagene; La Jolla, CA), sequenced on both strands with Sequenase II (U. S. Biochemical Corp., Cleveland, OH), and utilized for RNase protection, in situ hybridization, or genomic screening as described below. ..

    other:

    Article Title: SWM1 , a Developmentally Regulated Gene, Is Required for Spore Wall Assembly in Saccharomyces cerevisiae
    Article Snippet: 2118 on June 21, 2016 by U N IV O F S U S S E X http://m cb.asm .org/ D ow nloaded from otide primers and cloning the PCR fragments in Bluescript SK(1) vector (Stratagene), yielding plasmid pSU3.

    Article Title: Binding of Ca2+ and Zn2+ to Human Nuclear S100A2 and Mutant Proteins
    Article Snippet: Construction of Wild Type and Mutated Expression Vectors—To replace one or both of the functional Ca21 N- or C-terminal domain) of wtS100A2 (GenBankTM/EMBL accession number M87068) by a canonical loop (EF-domain) of human a-PV, the human cDNA (GenBankTM/ EMBL accession number X63070) was amplified by PCR on PerkinElmer and Hybaid Omni-Gene thermal cyclers, using specific oligonucleotides spanning the sequence of the EF-site of PV and specific restriction sites for subsequent cloning into the Bluescript SK1-vector (Stratagene).

    Derivative Assay:

    Article Title: Cloning and expression throughout mouse development ofmfat1, a homologue of theDrosophila tumour suppressor genefat
    Article Snippet: Chromogenic reactions to visualise alkaline phosphatase were carried out either using NBT/BCIP or BM purple (BoehringerMannheim Biochemicals, Indianapolis, IN). .. Clone F2, 800 bp (derived from the mfat1 39 untranslated region) in Bluescript SK1 vector (Stratagene) was linearised using Bam H1. ..



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