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bluescript ii sk1 vector  (Agilent technologies)


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    Structured Review

    Agilent technologies bluescript ii sk1 vector
    Bluescript Ii Sk1 Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript+sk1+vector/pm09593731-36-9-13
    Average 90 stars, based on 1 article reviews
    bluescript ii sk1 vector - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Generation of precise point mutation mice by footprintless genome modification.
    Article Snippet: Point mutation mice are a key tool in the study of biological functions of genomic DNA sequences and the creation of human disease models.. These mice are produced by homologous recombination combined with site-specific recombinase, which allows removal of drug selection cassettes.. However, the methods currently available leave ectopic sequences in the “inactive” intron region of the targeted genome in addition to the desired mutation.

    Article Title: Induction of protective immune response against Streptococcus suis serotype 2 infection by the surface antigen HP0245.
    Article Snippet: The PCR product was then cloned into pBluescript II SK (1) (Stratagene, La Jolla, CA) and subjected to sequencing.

    Article Title: Identification and functional characterization of the promoter of the mouse sodium-activated sodium channel Na(x) gene (Scn7a).
    Article Snippet: Nax is a sodium channel, thought to be a descendant of the voltage-gated sodium channel family.. Nevertheless, Nax is not activated by voltage but rather by augmentation of extracellular sodium over 150 mM.. In the brain, it is localized to the circumventricular organs, important regions for salt and water homeostasis in mammals, where it operates as a sodium-level sensor of body fluid.

    Article Title: Use of novel selenomethionine-resistant yeast to produce selenomethionyl protein suitable for structural analysis.
    Article Snippet: The EcoRV/NaeI fragment containing HIS4 from pPIC9 (Invitrogen) was ligated into the corresponding sites of pBluescript II SK(1) (Stratagene, La Jolla, CA), yielding plasmid pBS-HIS4.

    Article Title: Preprodynorphin-expressing neurons constitute a large subgroup of somatostatin-expressing GABAergic interneurons in the mouse neocortex.
    Article Snippet: Combined labeling of immunofluorescence staining and fluorescent in situ hybridization The complementary DNA fragment corresponding to a region of glutamic acid decarboxylase 67 kDa isoform (GAD67; nucleotides 43–661 in GenBank accession no. Y12257; Tamamaki et al., 2003) or vesicular glutamate transporter 1 (VGluT1; nucleotides 855-1788 in GenBank XM_133432.2; Nakamura et al., 2007) was cloned into pBluescript II SK (1) vector (Stratagene, La Jolla, CA).

    Article Title: Identification of Streptococcus suis genes preferentially expressed under iron starvation by selective capture of transcribed sequences.
    Article Snippet: Escherichia coli DH5a was used as the host strain for the construction and maintenance of the 16S and 23S rRNA genes prepared in pBluescript II SK(1) (Stratagene) and all SCOTS clones prepared in pMD-19T vector (TaKaRa, Dalian, China).

    Cloning:

    Article Title: Isolation and characterization of bacteriophage PhiBP from Paenibacillus polymyxa CCM 7400.
    Article Snippet: .. Escherichia coli XL1 Blue (Stratagene) was used as a host for cloning experiments and plasmid pBluescript II SK1 (Stratagene) was used as a cloning vector. ..

    Plasmid Preparation:

    Article Title: Isolation and characterization of bacteriophage PhiBP from Paenibacillus polymyxa CCM 7400.
    Article Snippet: .. Escherichia coli XL1 Blue (Stratagene) was used as a host for cloning experiments and plasmid pBluescript II SK1 (Stratagene) was used as a cloning vector. ..

    Article Title: A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme.
    Article Snippet: .. The gfp-modified hup-operon PCR product was cloned into the pBluescript II SK1 plasmid (Stratagene) before subcloning into pSUN119 (Argueta et al., 2004) using SmaI and SacI (Fermentas), generating plasmid pSHG. ..

    Polymerase Chain Reaction:

    Article Title: A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme.
    Article Snippet: .. The gfp-modified hup-operon PCR product was cloned into the pBluescript II SK1 plasmid (Stratagene) before subcloning into pSUN119 (Argueta et al., 2004) using SmaI and SacI (Fermentas), generating plasmid pSHG. ..

    Clone Assay:

    Article Title: A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme.
    Article Snippet: .. The gfp-modified hup-operon PCR product was cloned into the pBluescript II SK1 plasmid (Stratagene) before subcloning into pSUN119 (Argueta et al., 2004) using SmaI and SacI (Fermentas), generating plasmid pSHG. ..

    Subcloning:

    Article Title: A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme.
    Article Snippet: .. The gfp-modified hup-operon PCR product was cloned into the pBluescript II SK1 plasmid (Stratagene) before subcloning into pSUN119 (Argueta et al., 2004) using SmaI and SacI (Fermentas), generating plasmid pSHG. ..



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